scholarly journals Acute toxicity of Galium odoratum to the freshwater cladoceran Moina macrocopa

2021 ◽  
Vol 22 (3) ◽  
pp. 49-55
Author(s):  
Mariam Bozhilova
Keyword(s):  
Experimental Data ◽  
Acute Toxicity ◽  
Water Extract ◽  
Modern Medicine ◽  
Tribulus Terrestris ◽  
Matricaria Chamomilla ◽  
Moina Macrocopa ◽  
Lc50 Value ◽  
Galium Odoratum ◽  
Toxicity Effects

Galium odoratum (L.) is a medicinal plant with a number of health benefits, used in traditional and modern medicine. The toxicity of the coumarin in Galium odoratum is classified as high, however, no experimental data or data on toxic effects in humans following intake of Galium odoratum are available. The potential toxic effect can be estimated based on the content of coumarin and knowledge of its toxicity. The aim of the current study is to evaluate the acute toxicity effects of a range of concentrations of Galium odoratum water extract on Moina macrocopa and calculate the LC50 within 24 hours. In order to compare the toxicity with those of other, well-known and widely used medicinal plants, extracts of Matricaria chamomilla and Tribulus terrestris are also tested.  The results show that LC50 value of Galium odoratum is comparable with those of Matricaria chamomilla and Tribulus terrestris, and Galium odoratum has intermediate toxicity between the two other studied species. 

Acute Toxicity of Monochloramine to Juvenile Chinook Salmon (Oncorhynchus tshawytscha Walbaum) and Ceriodaphnia dubia

2001 ◽  
Vol 36 (1) ◽  
pp. 133-149 ◽  
Author(s):  
Anthony P. Farrell ◽  
Christopher Kennedy ◽  
Winnie Cheng ◽  
M. Anna Lemke
Keyword(s):  
Experimental Data ◽  
Acute Toxicity ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Ceriodaphnia Dubia ◽  
Aquatic Invertebrate ◽  
Data Set ◽  
Juvenile Chinook Salmon ◽  
Lc50 Value ◽  
Juvenile Chinook

Abstract A comprehensive data set for the acute toxicity of monochloramine to juvenile chinook salmon, Oncorhynchus tshawytscha, and the aquatic invertebrate Ceriodaphnia dubia are presented. For exposures up to 10 days, the equation LC50 = 7244t-0.4525 (where LC50 = monochloramine concentration in μg/L for 50% lethality and t = time in minutes) can predict the LC50 value for monochloramine in juvenile chinook salmon and accounts for 94.4% of the variability in the experimental data. Predictions of acute toxicity were less reliable for concentrations in the range 0.5 mg/L to 0.8 mg/L since the time to 50% mortality (LT50) value varied substantially in this range. Post-exposure mortality occurred only with exposures of >0.67 mg/L monochloramine. Feeding fish immediately before and during acute monochloramine exposure created a large chloramine demand in test chambers. In all tests where fish were fed, measured monochloramine concentrations were below detectable levels (<10 μg/L) and no fish mortality occurred. Monochloramine toxicity to the freshwater invertebrate Ceriodaphnia dubia was studied with time-to-lethality tests at 26 different concentrations. The equation LC50 = 61600t-1.0748 predicted the LC50 value for monochloramine in C. dubia and accounted for 94.8% of the variability in the experimental data. The mathematical equations that describe the LC50 for juvenile chinook salmon and C. dubia were used as reference lines for the evaluation of relative monochloramine toxicity. Both juvenile chinook salmon and C. dubia were among the most sensitive fish and aquatic invertebrate species, respectively, C. dubia being more sensitive than juvenile chinook salmon for exposure durations >30 minutes.

Toxic effects of sub lethal concentration of dioctyl phthalate on the histology of liver of Clarias batrachus (Linn.).

2016 ◽  
Vol 5 (09) ◽  
pp. 4874
Author(s):  
Manisha Satpathi* ◽  
Ravinder Singh
Keyword(s):  
Acute Toxicity ◽  
Light Microscopy ◽  
Dioctyl Phthalate ◽  
Clarias Batrachus ◽  
Fresh Water Fish ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Water Fish ◽  
Toxicity Effects ◽  
Plastic Products

Dioctyl phthalate (DOP) commonly used as plasticizer enters into aquatic environment from the industries manufacturing plastic products, PVC resins, cosmetics and many other commercial products. Therefore, this study was designed to evaluate the acute toxicity effects of Dioctyl phthalate on fresh water fish Clarias batrachus. The 96 hour LC50 of Dioctyl phthalate in Clarias batrachus was estimated as 14.5ml/L. Histopathological changes in liver of Clarias batrachus were determined by exposing them to a fraction of LC50(1/5th) dose with every change of water for 30 days.The liver of Clarias batrachus was excised at every interval of 30 days and histological changes of liver were studied under light microscopy. Liver tissues showed abnormalities. Centrilobular vacuolation, necrosis, eccentric nuclei and enlarged nuclei, Centrilobular degeneration of hepatocytes were observed in liver tissue of fish.

scholarly journals How much do the conventional parameters contribute to the biological toxicity of surface water in different types of villages?

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Ying Shao ◽  
Yueting Fan ◽  
Yinjie Yang ◽  
Min Zhou ◽  
Tingting Li ◽  
...  
Keyword(s):  
Water Quality ◽  
Surface Water ◽  
Acute Toxicity ◽  
Correlation Analysis ◽  
Surface Water Quality ◽  
Rural Environment ◽  
Environment Management ◽  
Biological Toxicity ◽  
Monitoring Programs ◽  
Toxicity Effects

Abstract Background In rural areas, the surface water quality is seriously threatened by pollution from agriculture, breeding, industrial and tourism activities. Even though many strategies and programs were launched for rural environment management, it is challenging to tackle the serious surface water pollution in villages. Since pollution status varies with the type of villages, there is no single parameter that defines environment quality completely. Until recently, most of rural surface water quality monitoring programs focus only on the conventional parameters, while can these conventional parameters reflect comprehensive status of water quality? To study how much the conventional parameters contribute to biological toxicity of surface water in villages, the in situ parameters of pH, DO, EC, ORP, laboratorial parameters of COD, TN, NO3−-N, NO2−-N, NH4+-N, TP, and PO43−-P and acute toxicity were analyzed for the surface water samples those were collected from six types of villages, named breeding, agricultural, handicraft, industrial, agricultural and breeding, and tourism. The correlation analysis was applied to establish the linkages and contributions of each parameter. Results The results showed that all detected surface waters were alkaline, and the concentration of TN and most of COD concentrations in all six types of villages were higher than the Class V limits specified in environmental quality standards for surface water (GB3838-2002). Pearson’s correlation analysis showed that significantly positive correlations were found for the acute toxicity effects and laboratorial parameters of NO2−-N, NH4+-N and PO43−-P in agricultural villages, and negative correlations were found for the acute toxicity effects and laboratorial parameters of TN and NO3−-N. No significant correlation was observed in breeding villages and industrial villages. Conclusion The problems of nitrogen pollution and aerobic pollution are still serious in villages, and more attention should be paid in further rural environment management. NH4+-N could contribute to the acute toxicity of surface water in the most of investigated villages, while no significant correlation was observed between acute toxicity and conventional parameters in industrial villages and tourism villages. Environmental monitoring programs focusing just on the classical conventional parameters are far from sufficient, since the main toxic contributors are quite different in diverse villages. The outcomes of the present study contribute to demonstrate the performance and usefulness of bioanalytical techniques for water quality assessment.

scholarly journals UJI TOKSISITAS AKUT MINUMAN PROBIOTIK SIRSAK GUNUNG (Annona montana Macf.) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 1 (5) ◽  
pp. 941-948
Author(s):  
Ambar Fidyasari ◽  
Sentot Joko Raharjo ◽  
Melani Setyowati
Keyword(s):  
Acute Toxicity ◽  
Traditional Medicine ◽  
Brine Shrimp ◽  
Experimental Methods ◽  
Test Method ◽  
Toxic Effects ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
A Value ◽  
Acute Toxic

Soursop fruit (Annona montana Macf.) is one of the plants can be used as as traditional medicine. This plant contains terpenoid and acetogenin which can cause toxicity. The fruit has a flavor that is tasteless so the innovation becomes probiotic drinks. This drink has been proven as an antioxidant, antibacterial, antihyperuricemia and antidiarrheal. The aim of this study was to know about acute toxicity of probiotic drink of soursop juice using brine shrimp lethality test method which will be indicated by LC50 value. This study used experimental methods conducted in the Laboratory of Farmakoknosi. There are several variations in concentration in this study, namely 10000 ppm, 20000 ppm, 30000 ppm, 40000 ppm, 50000 ppm, 60000 ppm, 70000 ppm, 80000 ppm and replication was done 3 times with total number of test animals used was 270. The results showed that probiotic drink of soursop juice can provide acute toxic effects on test animals with LC50 value of 29717,23 ppm. LC50 values ​​indicate that the mountain soursop probiotic drink is not potentially toxic because it has a value of >1000 ppm.

scholarly journals Toxicity Effects of Selected Heavy Metals on Lactuca sativa and Hydra viridissima used for Sustainable Crop Production

2018 ◽  
Vol 7 (3) ◽  
pp. 82
Author(s):  
Lina Maria Caceres Diaz ◽  
Claudia Campos ◽  
Gideon Oron
Keyword(s):  
Heavy Metals ◽  
Acute Toxicity ◽  
Lactuca Sativa ◽  
Toxicity Test ◽  
Crop Production ◽  
Arid Regions ◽  
Toxicity Assessment ◽  
Agricultural Irrigation ◽  
Lactuca Sativa L ◽  
Toxicity Effects

This study examines the prospects for using Hydra viridissima toxicity test 96-h LC50, served as a model invertebrate, Lactuca sativa L. was applied for seeds toxicity test (120-h IC50) and a model plant for an acute toxicity assessment of heavy metals content in water. The heavy metals used to assess the acute toxicity of the water utilized for agricultural irrigation in arid regions includes cadmium (CdCl2.2H2O), chromium (K2Cr2O7), zinc (ZnSO4. 7H2O), and boron (H3BO3). A grading of the substances was conducted, and it was found that the toxicity levels for H. viridissima and L. sativa were, with the least harmful first: B < Cr < Zn < Cd and Zn < B < Cr < Cd, respectively. Results indicate that H. viridissima was a more sensitive indicator of toxicity for all of the evaluated substances. However, L. sativa could also be used successfully to rank toxicants in order of their potential hazards.

scholarly journals Acute and Subchronic Toxicity Profile of a Polyherbal Drug Used in Sri Lankan Traditional Medicine

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Donisha Shani Niharika Keembiya Liyanagamage ◽  
Susanthi Jayasinghe ◽  
Anoja Priyadarshani Attanayake ◽  
Veranja Karunaratne
Keyword(s):  
Acute Toxicity ◽  
Glucose Concentration ◽  
Wistar Rats ◽  
Water Extract ◽  
Diabetic Rats ◽  
Acetone Extract ◽  
Hot Water ◽  
Piper Nigrum ◽  
Hot Water Extract ◽  
Acetone Extracts

A polyherbal drug composed of leaves of Murraya koenigii L. Spreng, cloves of Allium sativum L., fruits of Garcinia quaesita Pierre, and seeds of Piper nigrum L. is a popular drug which has been used by indigenous practitioners in Sri Lanka for the treatment of diabetes mellitus and dyslipidemia. The acute toxicity assessment was conducted, following a single oral dose of 0.25–2.0 g/kg in healthy rats, and rats were observed up to 14 days. The hot water extract (1.0 g/kg) and the water : acetone extract (0.5, 1.0, and 1.5 g/kg) were administered to Wistar rats for 28 days in the subchronic study. Hypoglycemic and antihyperglycemic activities (dose response studies) of cold water, hot water, and water : acetone extracts of the polyherbal mixture were evaluated at the doses of 0.5, 1.0, and 1.5 g/kg in healthy and streptozotocin-induced diabetic rats (70 mg/kg, ip), respectively. Acute toxicity study showed that the polyherbal drug did not cause any change in animals throughout the experimental period of 14 days. The administration of the hot water extract and the water : acetone extract of the polyherbal drug for 28 days did not produce changes in the selected biochemical and hematological parameters in Wistar rats (p > 0.05). The histological assessment corroborated the biochemical findings with no significant treatment-related changes in the kidney and liver. The treatment of polyherbal drug significantly lowered the serum glucose concentration compared to the diabetic control rats (p < 0.05) while it did not lead to a severe reduction of glucose concentration in healthy rats. The hot water and water : acetone extracts of the polyherbal drug showed a statistically significant improvement on total area under the glucose tolerance curve in diabetic rats (p < 0.05), reflecting dose-dependent antihyperglycemic effects of the drug. Based on the results, we conclude that the aforementioned antidiabetic polyherbal remedy is free of toxic/adverse effects at the equivalent human therapeutic dose in healthy Wistar rats and would be a safe therapeutic agent for long-term treatments.

scholarly journals Analisis Toksisitas Akut Ekstrak Etanol Kulit Buah Cempedak pada Tikus Galur Wistar

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Ali Rakhman Hakim ◽  
Rina Saputri
Keyword(s):  
Public Health ◽  
Heart Rate ◽  
Phenolic Compounds ◽  
Acute Toxicity ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Plant Parts ◽  
Fruit Skin ◽  
Public Health Protection ◽  
Toxicity Effects

Kulit buah Cempedak yang digoreng (Manday) merupakan salah satu makanan yang umum dikonsumsi masyarakat di Kalimantan Selatan. Berdasarkan pengalaman dari beberapa masyarakat, keluhan yang sering terjadi adalah perut terasa perih dan kepala pusing. Evaluasi sifat toksik dari bagian tumbuhan yang dikonsumsi oleh masyarakat sangat penting untuk dilakukan dengan mempertimbangkan perlindungan kesehatan masyarakat karena paparan senyawa kimia dari bagian tumbuhan yang dapat menyebabkan efek yang tidak diinginkan pada masyarakat. Penelitian ini bertujuan untuk mengidentifikasi senyawa kimia dan efek toksisitas akut dari ekstrak etanol kulit buah cempedak. Kulit buah cempedak dikeringkan dengan menggunakan oven. Ekstraksi kulit buah cempedak menggunakan cara maserasi dengan cairan penyari etanol. Tikus uji diberi larutan kontrol Na CMC dan ekstrak dengan dosis 300 mg/kgBB, 1000 mg/kgBB, 2000 mg/kgBB, dan 5000 mg/kgBB. Hasil identifikasi senyawa kimia didapatkan positif mengandung triterpenoid, flavonoid, tanin, dan senyawa fenolik. Tikus uji yang menerima ekstrak dengan dosis 1000 mg/kgBB, 2000 mg/kgBB, dan 5000 mg/kgBB terjadi perubahan pada tikus uji berdasarkan pengamatan fisik yaitu mengalami peningkatan detak jantung atau takikardi, pernapasan cepat, tremor, dan agresif. Kata kunci: Kulit buah cempedak, senyawa kimia, toksisitas akut Rind of Cempedak fruit (Manday) is one of the foods commonly consumed by people in South Kalimantan. Based on the experience of several communities, complaints that often occur are sore stomach and headache. Evaluation of the toxic properties of plant parts consumed by the community is very important to be done by considering public health protection due to exposure to chemical compounds from parts of plants that can cause undesirable effects on the community. This study aims to identify chemical compounds and acute toxicity effects of ethanol extract rind of cempedak fruit. Rind of Cempedak fruit has dried using an oven. Extraction of cempedak fruit skin using maceration method with ethanol. Test rats were given Na CMC as a control and extract with dose 300 mg / kgBB, 1000 mg / kgBB, 2000 mg / kgBB, and 5000 mg / kgBB. The results of the identification of chemical compounds were obtained positively containing triterpenoids, flavonoids, tannins, and phenolic compounds. The test rats that received extracts at a dose of 1000 mg / kgBW, 2000 mg / kgBB, and 5000 mg / kgBW were changed in the test rats based on physical observations that experienced an increase in heart rate or tachycardia, rapid breathing, tremor, and aggressive. Keywords: acute toxicity, chemistry compounds, rind of cempedak fruit

scholarly journals Acute Toxicity of NH4Cl to Tambra Fish (Tor Tambroides) Fry

2021 ◽  
Vol 2 (2) ◽  
pp. 49-55
Author(s):  
Gunawan Pratama Yoga ◽  
Kristin Noni Riyanti Buraen ◽  
Sutanto Sutanto
Keyword(s):  
Acute Toxicity ◽  
Preliminary Test ◽  
Ammonia Nitrogen ◽  
Test Method ◽  
Ammonia Toxicity ◽  
Lc50 Value ◽  
Total Ammonia Nitrogen ◽  
Test Kit ◽  
Effect Concentration ◽  
Total Ammonia

Ammonia is one of the nutrient wastes products which can be toxic to all vertebrates, including fish. Usually, ammonia toxicity is expressed as Total Ammonia Nitrogen (TAN, mgN/L). Tambra fish (Tor tambroides) is freshwater fish with important economic value in some areas in Indonesia. According to overfishing and forest degradation, the fish has become threatened. This research aimed to calculate the LC50 value of ammonia to Tambra fish (Tor tambroides) and estimate  NOEC,  LOEC, and MATC values of ammonia to Tambra fish (Tor tambroides). This study was conducted using the static test method with two steps. The first stage is a preliminary test, where the preliminary test is observed for 24 hours With five concentrations excluding control. Whereas in the second stage that is carried out, observation for 96 hours with four concentrations excluding control. The Probit test is Performed to obtain LC50 value results, then proceed with ammonia analysis using the Salicylate Test Kit method. The results showed that ammonia had acute toxicity with LC50-96 hour value of NH4Cl against Tambra fish fry (Tor tambroides) in the amount of 354.615 mg/L. Ammonia caused acute toxicity to Tambra fish fry (Tor tambroides).The LC50-96 hour value was 354.615 mg/L in the form of Total Ammonia Nitrogen (TAN) or it was equivalent to un-ionized Ammonia (NH3) of 9.8937 mg/L, while values of the Lowest Observed Effect Concentration (LOEC) and No Observed Effect Concentration (NOEC) of ammonia on Tambra fish (Tor tambroides) were 578.24 mg/L and 280.18 mg/L, respectively.

scholarly journals Guanicid and PHMG Toxicity Tests on Aquatic Organisms

2016 ◽  
Vol 64 (1) ◽  
pp. 129-134
Author(s):  
Eva Poštulková ◽  
Radovan Kopp
Keyword(s):  
Acute Toxicity ◽  
Green Algae ◽  
Danio Rerio ◽  
Guanidine Hydrochloride ◽  
Aquatic Organisms ◽  
Toxicity Tests ◽  
Freshwater Algae ◽  
Lc50 Value ◽  
Desmodesmus Communis ◽  
Cyanobacteria And Algae

The emergence and development of new algicidal products is caused by the ever increasing popularity of garden ponds as well as the use of these products in the fisheries sector, especially for disposal of cyanobacteria and algae. Most frequent means of combating cyanobacteria and algae are applications of algicidal substances. Newly developed algaecides include Guanicid and polyhexamethylene guanidine hydrochloride (PHMG). The aim of the study was to identify toxic effects of Guanicid and PHMG on zebrafish (Danio rerio) and green algae (Desmodesmus communis). We determined the acute toxicity in fish according to ČSN EN ISO 7346-1, and conducted the freshwater algae growth inhibition test according to ČSN ISO 8692 methodology. For inhibition tests with green algae we chose Guanicid and PHMG concentrations of 0.001, 0.005, and 0.010 ml/L. For fish short-term acute toxicity tests we chose Guanicid concentrations of 0.010, 0.050, 0.150, 0.200, 0.250, and 0.300 ml/L and PHMG concentrations of 0.010, 0.025, 0.050, 0.075, 0.100, and 0.125 ml/L. In case of zebrafish (Danio rerio), the LC50 value for Guanicid is 0.086 ml/L, while the LC50 value for PHMG is 0.043 ml/L. Effects of Guanicid on inhibition of green algae (Desmodesmus communis) appear highly significant (p < 0.010) at a concentration of 0.010 ml/L. For PHMG, these effects are highly significant (p < 0.001) at concentrations of 0.005 and 0.010 ml/L in 48 hours.

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