Antibacterial Activity and Probiotic Potential of Lactobacillus plantarum HKN01: A New Insight into the Morphological Changes of Antibacterial Compound-Treated Escherichia coli by Electron Microscopy

Treatment of pandrug resistant (PDR) Escherichia coli strain is the leading causative agent of bovine mastitis worldwide. Hence, becoming a potential threat to veterinary and public health. Therefore, to control the infection new nontoxic, biocompatible antimicrobial formulation with enhanced antibacterial activity is massively required. Current study was planned to synthesize chitosan coated titanium dioxide nanoparticles (CS-NPs coated TiO2). Coating was being done by chitosan nanoparticles (CS-NPs) using ionic gelation method. Aqueous solution of Moringa concanensis leaf extract was used to synthesize titanium dioxide nanoparticles (TiO2 NPs). The synthesized nanoformulations were characterized by using XRD, SEM, and FTIR. X-ray diffraction (XRD) analysis indicated the crystalline phase of TiO2 NPs and CS-NPs coated TiO2 NPs. Scanning Electron Microscopy (SEM) confirmed spherical shaped nanoparticles size of chitosan NPs ranging from 19–25 nm and TiO2 NPs 35–50 nm. Thesize of CS-NPs coated TiO2 NPs was in the range of 65–75 nm. The UV-Vis Spectra and band gap values illustrated the red shift in CS-NPs coated TiO2 NPs. Fourier transform infrared (FTIR) spectroscopy confirmed the linkages between TiO2 NPs and chitosan biopolymer, Zeta potential confirmed the stability of CS-NPs coated TiO2 NPs by showing 95 mV peak value. In-vitro antibacterial activity of CS-NPs coated TiO2 NPs and Uncoated TiO2 NPs was evaluated by disc diffusion method against PDR strain of E. coli isolated from mastitic milk samples. The antibacterial activity of all the synthesized nanoformulations were noted and highest antibacterial activity was shown by CS-NPs coated TiO2-NPs against pandrug resistant (PDR) E. coli strain with the prominent zone of inhibition of 23 mm. Morphological changes of E. coli cells after the treatment with MIC concentration (0.78 μg/ml) of CS-NPs coated TiO2 NPs were studied by transmission electron microscopy TEM showedrigorous morphological defectand has distorted the general appearance of the E. coli cells. Cytotoxicity (HepG2 cell line) and hemolytic (human blood) studies confirmed nontoxic/biocompatible nature of CS-NPs coated biologically synthesized TiO2 NPs. The results suggested that biologically synthesized and surface modified TiO2 NPs by mucoadhesive polysaccharides (e.g. chitosan) coating would be an effective and non-toxic alternative therapeutic agent to be used in livestock industry to control drug resistant veterinary pathogens.

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In Vitro Antibacterial Activity and Mechanism of Monocaprylin against Escherichia coli and Staphylococcus aureus

Journal of Food Protection ◽

10.4315/0362-028x.jfp-18-248 ◽

2018 ◽

Vol 81 (12) ◽

pp. 1988-1996 ◽

Cited By ~ 6

Author(s):

JIANYU WANG ◽

MAOMAO MA ◽

JUN YANG ◽

LONG CHEN ◽

PING YU ◽

...

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Membrane Potential ◽

Cell Membrane ◽

Sodium Benzoate ◽

Rapid Change ◽

Potassium Sorbate ◽

E Coli

ABSTRACT In the present study, the antibacterial activity of monocaprylin in comparison with sodium benzoate and potassium sorbate against Staphylococcus aureus and Escherichia coli was assessed by measuring MIC, MBC, effect of pH on MIC, and incubation temperature on bactericidal efficacy. Results showed that monocaprylin exhibited an excellent antibacterial activity against both strains, with the lowest MIC and MBC of 1.28 mg/mL. A MIC of monocaprylin remained unchanged despite the pH values of culture medium, ranging from 5 to 9, unlike that of potassium sorbate or sodium benzoate. Furthermore, monocaprylin at MBC effectively reduced the population of E. coli and S. aureus by >5.5 log CFU/mL at 25°C within 6 h and decreased E. coli by approximately 5.0 log CFU/mL and S. aureus by 2.9 log CFU/mL at 12 h. The underlying mechanism of monocaprylin was then investigated by measuring β-galactosidase activity, membrane potential, release of cellular contents, scanning electron microscopy, and transmission electron microscopy observations. Results indicated that monocaprylin killed E. coli by the rapid change in permeability and integrity of cell membrane, leading to decline of membrane potential, leakage of nucleic acids and proteins, and ultimately cell membrane disintegration and lysis. On the other hand, monocaprylin might exert its antibacterial activity against S. aureus mainly by diffusing across the cell wall, collapsing the cell membrane, and disturbing the order of intracellular contents. These findings indicated that monocaprylin had better antibacterial ability compared with traditional synthetic preservatives and might be a potential antibacterial additive independent of pH.

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Structure of plantaricin locus of Lactobacillus plantarum 8P-A3

Beneficial Microbes ◽

10.3920/bm2011.0030 ◽

2011 ◽

Vol 2 (4) ◽

pp. 255-261 ◽

Cited By ~ 13

Author(s):

A. Tsapieva ◽

N. Duplik ◽

A. Suvorov

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Sequence Analysis ◽

Lactobacillus Plantarum ◽

Streptococcus Pyogenes ◽

Antagonistic Activity ◽

Wide Range ◽

Encoding Genes

Lactobacillus plantarum 8P-A3 is a strain which is well known on the Russian pharmaceutical market and it is included in several probiotic products. The strain has been widely used since 1973 but the mechanisms of its antibacterial activity were unknown. L. plantarum 8P-A3 expressed high antagonistic activity against the wide range of bacterial pathogens including Streptococcus agalactiae, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae strains. DNA sequence analysis of L. plantarum 8P-A3 genome revealed the presence of a complete plantaricin locus of about 20,000 bp encoding genes of at least two bacteriocins – plantaricins EF and NC8. The plantaricin locus found in L. plantarum 8P-A3 is homologous to the plantaricin cluster in L. plantarum J51.

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UJI POTENSI PROBIOTIK Lactobacillus plantarium SECARA IN-VITRO

ALCHEMY ◽

10.18860/al.v0i0.2298 ◽

2013 ◽

Cited By ~ 1

Author(s):

Anik Maunatin ◽

Khanifa Khanifa

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bile Salt ◽

Lactobacillus Plantarum ◽

Pathogenic Bacteria ◽

Growth Efficiency ◽

Salmonella Typhi ◽

Probiotic Potential ◽

Number Of Cells

Probiotic is a product containing non-pathogenic microbes live, which was given to animals or humans to fix the rate of growth, efficiency, and increase conversion ration of animal or human health by affecting positively the balance of the gut microbes and microbial pathogen control in the digestive tract. In order to potentially lactic acid bacteria as probiotic candidates must pass the test selection among others such as probiotic test resistance to acid pH, the bile salts, against pathogenic bacteria. This experimental research methods for descriptive use that aim to provide information about the probiotic potential of Lactobacillus plantarum was isolated from the small intestine Mojosari duck (Anasplathyrinchos) with the tests in vitro i.e. the resistance of Lactobacillus plantarum on the condition of the acidicpH (2, 3, and 4), bile salt concentration of 0.3% (b/v), inhibition of pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Salmonella typhi) The results showed that on pH 2 the number of cells of bacteria that lives was 4.3. 107 CFU/ml, pH 3 was 3.8. 109 CFU/ml and pH 4 was 2.7. 1010 CFU/ml, the resistance of Lactobacillus plantarum on bile salt showed the number of cells of bacteria that lives was 1.2. 109 CFU/ml.inhibition of the pathogenic bacteria showed thatLactobacillus plantarumcould inhibited strong on Escherichia coli and Staphylococcus aureus with diameter of zone was 12.7 mm and 13.3 mm but notSalmonellatyphi with diameter of zone was 9.3 mm.

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Structure-Function Relationship of Antibacterial Synthetic Peptides Homologous to a Helical Surface Region on Human Lactoferrin against Escherichia coli Serotype O111

Infection and Immunity ◽

10.1128/iai.66.6.2434-2440.1998 ◽

1998 ◽

Vol 66 (6) ◽

pp. 2434-2440 ◽

Cited By ~ 72

Author(s):

Daniel S. Chapple ◽

David J. Mason ◽

Christopher L. Joannou ◽

Edward W. Odell ◽

Vanya Gant ◽

...

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Transmission Electron Microscopy ◽

Flow Cytometry ◽

Antibacterial Activity ◽

Synthetic Peptides ◽

Membrane Integrity ◽

E Coli ◽

Transmission Electron ◽

Helical Region

ABSTRACT Lactoferricin includes an 11-amino-acid amphipathic alpha-helical region which is exhibited on the outer surface of the amino-terminal lobe of lactoferrin. Synthetic peptides homologous to this region exhibited potent antibacterial activity against a selected range of both gram-negative and gram-positive bacteria. An analog synthesized with methionine substituted for proline at position 26, which is predicted to disrupt the helical region, abolished antibacterial activity against Escherichia coli and considerably reduced antibacterial activity against Staphylococcus aureus and anAcinetobacter strain. The mode of action of human lactoferrin peptide (HLP) 2 against E. coli serotype O111 (NCTC 8007) was established by using flow cytometry, surface plasmon resonance, and transmission electron microscopy. Flow cytometry was used to monitor membrane potential, membrane integrity, and metabolic processes by using the fluorescent probes bis-1,3-(dibutylbarbituric acid)-trimethine oxonol, propidium iodide, and carbonyl cyanide m-chlorophenylhydrazone, respectively. HLP 2 was found to act at the cell membrane, causing complete loss of membrane potential after 10 min and of membrane integrity within 30 min, with irreversible damage to the cell as shown by rapid loss of viability. The number of particles, measured by light scatter on the flow cytometer, dropped significantly, showing that bacterial lysis resulted. The peptide was shown to bind toE. coli O111 lipopolysaccharide by using surface plasmon resonance. Transmission electron microscopy revealed bacterial distortion, with the outer membrane becoming detached from the inner cytoplasmic membrane. We conclude that HLP 2 causes membrane disruption of the outer membrane, resulting in lysis, and that structural considerations are important for antibacterial activity.

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Evaluation of the Antibacterial Activity and Probiotic Potential of Lactobacillus plantarum Isolated from Chinese Homemade Pickles

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2020/8818989 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Y. Zeng ◽

Y. Li ◽

Q. P. Wu ◽

J. M. Zhang ◽

X. Q. Xie ◽

...

Keyword(s):

Antibacterial Activity ◽

Lactic Acid ◽

Lactobacillus Plantarum ◽

Antibiotic Resistance Genes ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Bile Salt Hydrolase ◽

Rrna Gene ◽

Probiotic Potential

This study investigated the antipathogenic activity and probiotic potential of indigenous lactic acid bacteria (LAB) isolated from Chinese homemade pickles. In total, 27 samples were collected from different sites in China. Fifty-nine yielded pure colonies were identified by 16S rRNA gene sequencing as LAB and were initially evaluated for the antibacterial activity in vitro. Initial screening yielded Lactobacillus plantarum GS083, GS086, and GS090, which showed a broad-spectrum antibacterial activity against food-borne pathogens, especially multidrug-resistant pathogens. Meanwhile, organic acids were mainly responsible for the antimicrobial activity of the LAB strains, and the most abundant of these was lactic acid (19.32 ± 0.95 to 24.79 ± 0.40 g/l). Additionally, three L. plantarum strains demonstrated several basic probiotic characteristics including cell surface hydrophobicity, autoaggregation, and survival under gastrointestinal (GI) tract conditions. The safety of these isolates was also evaluated based on their antibiotic susceptibility, hemolytic risk, bile salt hydrolase activity, and existence of virulence or antibiotic resistance genes. All strains were safe at both the genomic and phenotypic levels. Therefore, L. plantarum GS083, GS086, and GS090 are fairly promising probiotic candidates and may be favorable for use as preservatives in the food industry.

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Characterization of an inositol-producing Lactobacillus plantarum strain and the assessment of its probiotic potential and antibacterial activity

LWT ◽

10.1016/j.lwt.2021.112553 ◽

2021 ◽

pp. 112553

Author(s):

Bing Fu ◽

Xingxing Huang ◽

Jian Ma ◽

Qingwei Chen ◽

Qili Zhang ◽

...

Keyword(s):

Antibacterial Activity ◽

Lactobacillus Plantarum ◽

Probiotic Potential

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SINTESIS DAN KARAKTERISASI NANOPARTIKEL CuFe2O4 SERTA APLIKASINYA SEBAGAI ANTIBAKTERI

CHEMISTRY PROGRESS ◽

10.35799/cp.12.2.2019.27929 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Almendo G. Lasera ◽

Henry Aritonang ◽

Harry Koleangan

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Gram Negative Bacteria ◽

E Coli ◽

Activity Test ◽

Sem Imaging ◽

Scanning Electron

ABSTRAKNanopartikel CuFe2O4 disintesis menggunakan metode kopresipitasi. Material yang telah disintesis, kemudian dikarakterisasi dengan menggunakan Scanning Electron Microscopy (SEM) dan Xray-Diffraction (XRD) serta di uji aktifitasnya sebagai antibakteri dengan menggunakan bakteri Staphylococcus aureus (Gram positif) dan Escherichia coli (Gram negatif). Hasil citra SEM memperlihatkan morfologi nanopartikel CuFe2O4 berbentuk persegi yang tidak beraturan. Difraktogram XRD menunjukkan bahwa ukuran sampel CuFe2O4 yang diperoleh adalah sebesar 20,136 nm. Uji aktifitas antibakteri yang telah dilakukan menunjukkan bahwa, nanopartikel CuFe2O4 jauh lebih baik dalam menghambat pertumbuhan bakteri E. coli dibandingkan dengan bakteri S.aureus, masing-masing 24 mm dan 6,5 mm. ABSTRACTCuFe2O4 nanoparticles were synthesized using the coprecipitation method. The synthesized material was then characterized using Scanning Electron Microscopy (SEM) and Xray-Difraction (XRD) and tested as antibacterial activity using Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) bacteria. SEM imaging showed morphology of CuFe2O4 nanoparticles shaped like a rod. XRD diffractogram showed that the size of CuFe2O4 nanoparticles that obtained at 20.13 nm. The antibacterial activity test that has been done shows, CuFe2O4 nanoparticles are much better in inhibiting the growth of E. coli bacteria compared with S. aureus bacteria, 24 mm and 6,5 respertivelly.

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Pengaruh Perbedaan Suhu Pemanasan terhadap Aktivitas Antibakteri Susu Kambing Fermentasi Menggunakan Lactobacillus Plantarum

Jurnal Agripet ◽

10.17969/agripet.v19i2.14330 ◽

2019 ◽

Vol 19 (2) ◽

pp. 142-148

Author(s):

Zuraida Hanum ◽

Yurliasni Yurliasni ◽

Sitti Wajizah ◽

Juanda Juanda

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Lactobacillus Plantarum ◽

Heating Temperature ◽

Goat Milk ◽

Factor B ◽

E Coli ◽

Randomized Design ◽

Completely Randomized Design ◽

Inhibitory Power

ABSTRAK. Penelitian ini bertujuan untuk melihat pengaruh suhu pemanasan yang berbeda terhadap aktivitas antibakteri susu kambing fermentasi menggunakan starter Lactobacillus plantarum. Bakteri uji yang digunakan berasal dari golongan Enterobacteriaceae yaitu Escherichia coli dan Salmonella.sp. Rancangan penelitian yang digunakan adalah RAL (Rancangan Acak Lengkap) Faktorial yang terdiri dari 2 faktor yaitu faktor A adalah suhu pemanasan, yaitu a1 (suhu pemanasan 72°C), a2 (suhu pemanasan 121°C) dan faktor B adalah level penambahan Lactobacillus plantarum yaitu perlakuan b1 (Lactobacillus plantarum 2,5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7,5%), masing-masing perlakuan diulang sebanyak 3 kali sehingga diperoleh 18 unit percobaan. Apabila terdapat perbedaan yang nyata maka dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan adanya interaksi dari suhu pemanasan yang berbeda dan penambahan L. plantarum pada susu kambing berpengaruh nyata terhadap kadar asam laktat dan daya hambat pada Salmonella sp, akan tetapi tidak berpengaruh nyata terhadap E. coli. Kesimpulan dari penelitian ini diperoleh suhu pasteurisasi dengan penambahan 7.5 % L.plantarum merupakan nilai terbaik dalam menghambat Salmonella dan E..coli. (Effect of different heating temperature on antibacterial activity of goat milk fermented with Lactobacillus Plantarum) ABSTRACT. This study aimed to determine the effect of the different heating temperatures on inhibitory activity of fermented goat's milk with Lactobacillus plantarum. The test bacteria used were Enterobacteriaceae, namely Escherichia coli and Salmonella.sp. The design applied is CFD (Completely Randomized Design) consisting of 2 factors, namely factor A heating temperature with two levels a1 (72°C), a2 (121°C) and factor B is the level of Lactobacillus plantarum addition by three levels, namely b1 (Lactobacillus plantarum 2.5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7.5%), and the treatments were repeated 3 times. The results showed that different heating temperature and level of L. plantarum added in fermented goat milk significantly affected the levels of lactic acid and inhibitory power on Salmonella sp, but not on E. coli. In conclusion is different heating temperatures and addition of L. plantarum on goat milk at significantly affected on inhibitory power on Salmonella sp, but not on E. coli. The results obtained pasteurisasi with 7.5% L.plantarum could be applied.

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UJI ANTIBAKTERI RIMPANG GANDASULI (HEDYCHIUM CORONARIUM) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI DENGAN PERBANDINGAN METODE EKSTRAKSI

Journal of Pharmacopolium ◽

10.36465/jop.v1i2.326 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Aris Suhardiman

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Sulfuric Acid ◽

Acid Reflux ◽

Diffusion Method ◽

Hedychium Coronarium ◽

Antibacterial Compound ◽

Inhibition Zones ◽

Positive Effect

Indonesia has various types of plants those have potential as medicine to overcome health problems in line with the expanding of traditional medicine industry. One example of plants from the Zingiberaceae family is Hedychium coronarium, which is not widely known by the community despite it has efficacy as an antibacterial which is not inferior to other Zingiberaceae family. The research was conducted to determine whether the Hedychium coronarium rhizome shown to have antibacterial activity against the growth of Staphylococcus aureus and Escherichia coli, knowing antibacterial compound that contained in Hedychium coronarium rhizome, also knowing the most effective extraction method to take the antibacterial compound from Hedychium coronarium rhizome. The gandasuli rhizome was extracted by maceration and reflux with 96% ethanol. Antibacterial activity test carried out by the agar diffusion method, and the analysis of antibacterial compounds with bioautography, and the results are monitored with vanillin-sulfuric acid. Maceration and reflux extract at a concentration of 90% has antibacterial activity with inhibition zones, each for 8 and 10 mm against Escherichia coli as well as 9 and 10 mm against Staphylococcus aureus. Bioautography results show a positive effect on the appearance of vanillin-sulfuric acid. Reflux extract has better antibacterial activity, while the antibacterial compound is an essential oil group. Keywords: Hedychium coronarium, antibacterial, Staphylococcus aureus, Escherichia coli, bioautography.

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