Stability and Antioxidant Activity of Polyphenols in Methanolic Extracts of Sonneratia caseolaris Seeds

Polyphenols in methanolic extracts from Sonneratia caseolaris seeds were determined by HPLC coupled with electrospray mass spectrometry and quantified by HPLC coupled with ultraviolet/visible detection in order to evaluate their stability of the extracts during 6 months of storage. Antioxidant activity was measured by using TEAC assay, and the free radical scavenging activity was monitored in the initial 3 months of the stability evaluation. Study on the effect of temperature on the stability of the methanolic extract indicated that gallic acid, luteolin-7-O-glucoside, and luteolin were unstable at 25 and 45°C during storage time. At -80 and 4°C the quantities of luteolin-7-O-glucoside reduced while those of luteolin increased at the same time. The results suggested that luteolin-7-O-glucoside was hydrolyzed to luteolin. Therefore the polyphenols should be kept at low temperature. Antioxidant activity of methanolic extract was preserved in all temperatures during the first 3 months.

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In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn.

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20181424 ◽

2018 ◽

Vol 7 (5) ◽

pp. 849

Author(s):

Bindhu R. Kamath ◽

Sabeena Kizhedath

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Methanolic Extract ◽

Heart Diseases ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

In Vitro Study ◽

Cassia Fistula ◽

Methanolic Extracts

Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.

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Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

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ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i9.11954 ◽

2016 ◽

Vol 8 (9) ◽

pp. 293 ◽

Cited By ~ 3

Author(s):

Dasharath B. Shinde ◽

Santosh S. Koratkar ◽

Neeti Sharma ◽

Ajinkya A. Shitole

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Hepg2 Cells ◽

Phenolic Content ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Glycyrrhiza Glabra ◽

Total Phenolic ◽

Hepg2 Cell Line

Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2 induced oxidative stress in HepG2 cell line.Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glabra was investigated by measuring total phenolic content using folin-ciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of Glycyrrhiza glabra against oxidative stress induced by H2O2 in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H2O2, ascorbic acid, H2O2+liquorice and H2O2+ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels.Results: The total phenolic content estimated in Glycyrrhiza glabra extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H2O2 treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.Conclusion: Our results thus conclude that, the methanolic extract of Glycyrrhiza glabra can be used as natural supplements in various disease conditions where oxidative stress has been reported.

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Total Phenolic Content and DPPH Free Radical Scavenging Activity of Young Turmeric Grown in Southern Thailand

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.886.61 ◽

2019 ◽

Vol 886 ◽

pp. 61-69

Author(s):

Suriyan Sukati ◽

Warachate Khobjai

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Scavenging Activity ◽

Methanolic Extracts ◽

Southern Thailand

Turmeric (Curcuma longa L.), a member of Zingiberacaeae, is used for Thai traditional medicine, flavoring, preservative, and coloring agent. In Southern Thailand, turmeric is a famous ingredient for food recipes. Not only is the ripened rhizome used for cuisine, but also the young rhizomes. Previous studies have indicated that a variety of turmeric products from the ripened rhizome are valuable source of antioxidant compounds. However, there are ambiguous data regarding the level of antioxidant activity of the young rhizome. The aim of this study was to investigate the level of total phenolic content (TPC) and antioxidant activity of the young rhizomes by comparing to the ripened rhizome. TPC and antioxidant activity of aqueous and 80% methanolic extracts from the young turmeric grown in Southern Thailand were estimated using the Folin-Ciocalteu method and DPPH free radical scavenging activity (DPPH-RSA) assay, respectively. Results showed that the concentration of TPC determined in methanolic extract (19.80±0.25 mg GAE/ g extract) from the young rhizome was significantly higher than the corresponding aqueous extract (18.38±0.41 mg GAE/ g extract). The aqueous and methanolic extracts exhibited significant inhibition in DPPH-RSA with the half-maximal inhibitory concentration (IC50) value of 5.88±0.70 mg/mL and 3.00±0.31 mg/mL, respectively. However, in comparison with the ripened rhizome, TPC and DPPH-RSA of the young rhizome were lower. These results indicate that the young rhizome grown in Southern Thailand could be a source of antioxidant compounds, but the ripened rhizome provides better antioxidant properties than the young rhizome.

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Enhancement of Chemical Stability and Dermal Delivery of Cordyceps militaris Extracts by Nanoemulsion

Nanomaterials ◽

10.3390/nano10081565 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1565 ◽

Cited By ~ 1

Author(s):

Pachabadee Marsup ◽

Kankanit Yeerong ◽

Waranya Neimkhum ◽

Jakkapan Sirithunyalug ◽

Songyot Anuchapreeda ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Stability ◽

Radical Scavenging Activity ◽

Skin Irritation ◽

Radical Scavenging ◽

Water Extract ◽

Cordyceps Militaris ◽

Alpha Tocopherol ◽

Dermal Delivery ◽

The Stability

This study aimed to develop nanoemulsions for enhancing chemical stability and dermal delivery of Cordyceps militaris extracts. C. militaris was extracted by maceration and infusion. The extracts were investigated for cordycepin, phenolic, and flavonoid content. The antioxidant activity was investigated by in vitro spectrophotometric methods. The irritation profile was investigated by hen’s egg-chorioallantoic membrane test. Nanoemulsions were developed using high-pressure homogenizer. C. militaris extract was incorporated into the nanoemulsion and investigated for safety, release profile, permeation, and skin retention. The results demonstrated that water extract (CW) contained the significantly highest content of cordycepin, phenolics, and flavonoids, which were responsible for antioxidant activity. CW was the most potent antioxidant. CW possessed comparable 2,2′-diphenyl-1-picrylhydrazyl radical scavenging activity and lipid peroxidation inhibition to l-ascorbic acid (96.9 ± 3.1%) and alpha-tocopherol (87.2 ± 1.0%). Consequently, ten mg/mL of CW was incorporated into nanoemulsions composing of sugar squalene, Tween® 85, and deionized water. Nanoemulsion, which had the smallest internal droplet size (157.1 ± 2.6 nm), enhanced the stability of CW, had no cytotoxicity effect and no skin irritation, released the most CW (0.9 ± 0.0% w/w after 24 h), and delivered the highest CW into the skin layer (33.5 ± 0.7% w/w). Therefore, nanoemulsion was suggested for enhancing the stability and dermal delivery of CW.

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Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains

Journal of Food Quality ◽

10.1155/2017/2924508 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 46

Author(s):

Idaira Jerez-Martel ◽

Sara García-Poza ◽

Gara Rodríguez-Martel ◽

Milagros Rico ◽

Cristina Afonso-Olivares ◽

...

Keyword(s):

Antioxidant Activity ◽

High Performance ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Arthrospira Platensis ◽

Chlorogenic Acids ◽

Nostoc Commune ◽

Reverse Phase ◽

Phenolic Profile ◽

Methanolic Extracts

Aqueous and methanolic extracts of several microalgae (Ankistrodesmus sp., Spirogyra sp., Euglena cantabrica, and Caespitella pascheri) and cyanobacteria (Nostoc sp., Nostoc commune, Nodularia spumigena, Leptolyngbya protospira, Phormidiochaete sp., and Arthrospira platensis) were screened for their radical scavenging activity against the stable radical 1,1-diphenyl-2-picrylhydrazyl. Despite the fact that water was a more efficient solvent to extract greater amount of extractable substances, it seems that methanol was more efficient to extract a selected group of compounds with a higher antioxidant activity. In addition, the identification of 4 simple phenolics (gallic, syringic, protocatechuic, and chlorogenic acids) and the flavonoids (+) catechin and (-) epicatechin was carried out by using reverse phase high performance liquid chromatography. The strain Euglena cantabrica showed the highest concentration of phenolic compounds, particularly gallic and protocatechuic acids (5.87 and 2.97 mg per gram of dried biomass, resp.). Aqueous and methanolic extracts of microalgae Euglena cantabrica also exhibited the highest antioxidant activity, probably due to the presence of the high contents of phenolics.

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Degradation kinetics during storage of vitamins and antioxidants in coconut powder

DYNA ◽

10.15446/dyna.v87n213.80681 ◽

2020 ◽

Vol 87 (213) ◽

pp. 61-68

Author(s):

Juan Carlos Lucas Aguirre ◽

German Antonio Giraldo Giraldo ◽

Misael Cortés Rodríguez

Keyword(s):

Antioxidant Activity ◽

Storage Temperature ◽

Degradation Kinetics ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Storage Conditions ◽

Treatment Temperature ◽

Effect Of Temperature ◽

Order Kinetic ◽

Degradation Kinetic

Degradation kinetic parameters in vitamins and antioxidants in coconut powder fortified with functionally active compounds (CP+FAC) during storage represents a valuable tool to predict the product’s shelf life. The aim of this research was to evaluate the degradation kinetics during storage of vitamins (C, D3, and E), total phenols, and properties associated to antioxidant activity. Vitamins were quantified via high-resolution liquid chromatography and the antioxidant activity through DPPH and ABTS free radical scavenging activity. An experimental design was used with two-factor factorial design: 1) storage time (tS) and 2) treatment: temperature (TA) - type of packaging (N2 and atmospheric air), where the dependent variables were adjusted to zero-, first-, and second-order kinetic models. In general, losses of FAC were significant with respect to the factors evaluated and their interactions, with minor changes at lower TA and packaged with N2, indicating that the degradation kinetics of the FAC is slower under these storage conditions. Increased storage temperature reduced FACstability, where the Arrhenius equation was able to quantify the effect of temperature of the rate of deterioration reactions of the FAC.

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Comparative Study of the Antioxidant Activity and Phytochemical Composition of Leaves Extract between Three Varieties of Date Palm Tree

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.14.162 ◽

2013 ◽

Vol 14 ◽

pp. 162-171

Author(s):

Laouini Salah Eddine ◽

Ladjel Segni ◽

Ouahrani Mohamed Ridha

Keyword(s):

Antioxidant Activity ◽

Date Palm ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Point Of View ◽

Palm Tree ◽

Phytochemical Composition ◽

Methanolic Extracts ◽

The People ◽

Antioxidant And Antimicrobial Activity

The date palm (Phoenyx dactylifera) consisted, for the people of southern Algeria, as tree of providence. Dates and their extracts are also used for many centuries as a medicine against allergy, inflammation, constipation and gastro-protective; they also have a high antibacterial and antioxidant activity. However, no studies are conducted to evaluate the extract from the leaves of date palm (Phoenyx dactylifera) in point of view of phytochemical composition, antimicrobial and the antioxidant activity. In this study, we have determined the phenolic compounds, antioxidant and antimicrobial activity of methanolic extracts from three varieties of leaves tree. According to the results the leaves extracts have very important values for polyphenols (215.24 to 156.46 mg GAE/gDW) and high antioxidant activity (324. 45 to 206.21 mg GAE/gDW), Diphenyl-1 picrylhydrazyl radical-scavenging activity (IC50 = 2.98 to 4.83 μg/ml); also the three extracts reveal a considerable antimicrobial potency and antifungal considerable activity, the diameter of inhibition is 35.2 to 39.5 mm (concentration 50 mg/ml) for Candida albicans ATCC 90026.

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Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves

The Scientific World JOURNAL ◽

10.1155/2019/7549620 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Sabine Adeline Fanta Yadang ◽

Germain Taiwe Sotoing ◽

Katoucha Sidoine Ngatcha Zouakeu ◽

Muhammad Ahsan Khan ◽

Gabriel Agbor Agbor ◽

...

Keyword(s):

Antioxidant Capacity ◽

Bioactive Compounds ◽

Total Phenolic Content ◽

Hplc Analysis ◽

Phenolic Content ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Methanolic Extracts

Carissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases. The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compounds present in the various extracts of C. edulis (HEC, MEC, AEC, and PC). An HPLC analysis determined the different compounds present in the extracts. High concentration of total phenolic content was observed in aqueous and methanolic extracts more than in the hydroethanolic extract though not significantly different. Flavonoids were higher in the hydroethanolic and methanolic extracts, respectively, with 14.84 mg RE/g extract and 12.02 mg RE/g extract. Tannins were also found in large amounts in the same two extracts with 26.76 mg TAE/g extract and 34.67 mg TEE/g extract. The percentage radical scavenging activity DPPH ranged between 58.63% and 94.67% for aqueous extract and for ABTS between 51.39% and 94.12% for the methanolic extract. The highest FRAP was obtained in the methanolic extract (6.73 g AAE/100 g extract). HPLC analysis revealed the presence of quercetin, rutin, and gallic acid in the different extracts. C. edulis represents a potential source of bioactive components with antioxidant capacity.

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PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i5.22138 ◽

2017 ◽

pp. 59-63

Author(s):

Abdul Sadat ◽

Mayukh Hore ◽

Kaushik Chakraborty ◽

Subhrajyoti Roy

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Phytochemical Analysis ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

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