The Rehabilitation Project of Highland Road Tunnel

2011 ◽  
Vol 255-260 ◽  
pp. 1048-1051
Author(s):  
Hai Jing Liu ◽  
Jia Yan Zheng ◽  
Zhi Lin

This paper presents the rehabilitation project of Dabanshan highland road tunnel. Cause analysis is carried by insight into the information of climate, geological, hydrological condition, tunnel defects type and parameters. Water leakage, low temperature and poor rock condition are considered to be source of tunnel defects, and the essential of rehabilitation of highland road tunnel are water leakage treatment and heat preservation system reinstatement. Measures taken to solve the problems of cracks, water leakage and frost are introduced in detail, and results show that measures taken are proved to be effective. Knowledge and experience gained are helpful for similar projects in future.

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 43
Author(s):  
Marco Mangiagalli ◽  
Marina Lotti

β-galactosidases (EC 3.2.1.23) catalyze the hydrolysis of β-galactosidic bonds in oligosaccharides and, under certain conditions, transfer a sugar moiety from a glycosyl donor to an acceptor. Cold-active β-galactosidases are identified in microorganisms endemic to permanently low-temperature environments. While mesophilic β-galactosidases are broadly studied and employed for biotechnological purposes, the cold-active enzymes are still scarcely explored, although they may prove very useful in biotechnological processes at low temperature. This review covers several issues related to cold-active β-galactosidases, including their classification, structure and molecular mechanisms of cold adaptation. Moreover, their applications are discussed, focusing on the production of lactose-free dairy products as well as on the valorization of cheese whey and the synthesis of glycosyl building blocks for the food, cosmetic and pharmaceutical industries.


2014 ◽  
Vol 14 (3) ◽  
pp. 479-488 ◽  
Author(s):  
T. Backhaus ◽  
R. de la Torre ◽  
K. Lyhme ◽  
J.-P. de Vera ◽  
J. Meeßen

AbstractSeveral investigations on lichen photobionts (PBs) after exposure to simulated or real-space parameters consistently reported high viability and recovery of photosynthetic activity. These studies focused on PBs within lichen thalli, mostly exposed in a metabolically inactive state. In contrast, a recent study exposed isolated and metabolically active PBs to the non-terrestrial stressor UVC254 nm and found strong impairment of photosynthetic activity and photo-protective mechanisms (Meeßen et al. in 2014b). Under space and Mars conditions, UVC is accompanied by other stressors as extreme desiccation and low temperatures. The present study exposed the PBs of Buellia frigida and Circinaria gyrosa, to UVC in combination with desiccation and subzero temperatures to gain better insight into the combined stressors' effect and the PBs' inherent potential of resistance. These effects were examined by chlorophyll a fluorescence which is a good indicator of photosynthetic activity (Lüttge & Büdel in 2010) and widely used to test the viability of PBs after (simulated) space exposure. The present results reveal fast recovery of photosynthetic activity after desiccation and subzero temperatures. Moreover, they demonstrate that desiccation and cold confer an additional protective effect on the investigated PBs and attenuate the PBs' reaction to another stressor – even if it is a non-terrestrial one such as UVC. Besides other protective mechanisms (anhydrobiosis, morphological–anatomical traits and secondary lichen compounds), these findings may help to explain the high resistance of lichens observed in astrobiological studies.


2017 ◽  
Vol 2 (3) ◽  
pp. 390-396 ◽  
Author(s):  
Vinzent Strobel ◽  
Julian Jonathan Schuster ◽  
Andreas Siegfried Braeuer ◽  
Lydia Katharina Vogt ◽  
Henrik Junge ◽  
...  

A combination of operando Raman spectroscopy with online GC and volume-flow monitoring allows rapid insight into low-temperature methanol reforming.


Author(s):  
Marian Sikora ◽  
Janusz Gołdasz

The aim of this work is to provide an insight into the rattle noise phenomena occurring in double-tube (twin-tube) vehicle suspension dampers. In the dampers the particular phenomenon results from interactions between the valve(s) and the fluid passing through them. The rattling noise phenomena is known to degrade the vehicle passenger’s perception of ride comfort as well as to influence the performance of the dampers at low and medium speeds in particular. In the paper the authors reveal the results of a DOE (Design of Experiment) study involving several design parameters known to affect rattling occurrence. By running a series of purpose-designed tests the authors investigate not only the contribution of each particular parameter but the interactions between them. The results are presented in the form of pareto charts, main effect plots as well as interaction plots. It is expected the outcome of the analysis will aid in a better comprehension of the phenomena as well the definition of valve configurations to minimize their performance degradation.


Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3419
Author(s):  
He Zhou ◽  
Yuqing Sun ◽  
Xin Li ◽  
Ziyu Zhou ◽  
Kexin Ma ◽  
...  

The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of Takifugu rubripes, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of T. rubripes (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (LOC101066759, dgat1, limk1, fbxl3, col6a3, fgfr3, dusp22b, svil, abhd17b, srgap3, tmem88b, bud4, and mustn10) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for T. rubripes and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.


2021 ◽  
pp. 117529
Author(s):  
Akihiro Ishii ◽  
Hibiki Ishijima ◽  
Kosei Kobayashi ◽  
Itaru Oikawa ◽  
Hitoshi Takamura

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