The Use of 15N to Measure N2-Fixing Effectiveness of Rhizobium leguminosarum Strains

2011 ◽  
Vol 287-290 ◽  
pp. 2023-2027
Author(s):  
Ya Hui He ◽  
Gao Bao Huang ◽  
Li Zhuo Guo
Keyword(s):  
Plant Tissue ◽  
Rhizobium Leguminosarum ◽  
Plant Biomass ◽  
Biomass Accumulation ◽  
N Fixation ◽  
Total N ◽  
Whole Plant ◽  
Biomass N ◽  
Different Strains ◽  
Plant Biomass Production

Biomass, N derived from BNF, total N and Ndfa % of different part tissues of pea plants inoculated with various Rhizobium. leguminosarum strains were determined. Particularly identified were whole plant biomass correlated with N derived from BNF. The significant direct correlations between biomass and N derived from BNF indicated that N-fixation efficiency of strains are important factors influencing biomass accumulation of plants, but not the sole factors determine the promoted capacity of strain on plant biomass production. Different strains show various performances on accumulation of biomass in different parts of plant tissue, R. leguminosarum SY12 performed best on promotion of kernel production. In order to obtain aimed strain with particular property such as promotion of kernel production, the15N tracing technique can be used in R. Leguminosarum strains screening of R. Leguminosarum, but the analysis both separate part and whole plant tissue are necessary.

scholarly journals The Effect of Nutrient Regime on Biomass Allocation and Nutrient Accumulation in Red Oak Seedlings

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 859F-859
Author(s):  
Jill C. Larimer ◽  
Dan Struve
Keyword(s):  
Biomass Allocation ◽  
Plant Biomass ◽  
N Fertilizer ◽  
Water Soluble ◽  
Fertilizer Treatment ◽  
Total N ◽  
Whole Plant ◽  
Total Plant Biomass ◽  
Total Plant ◽  
Stems And Leaves

ln Spring 1993, red oaks (Quercus rubra) were propagated from seed. From June through October, plants were fertilized twice daily with 1.4 liters of 20N–10P–20K water-soluble fertilizer solution at concentrations of 0, 25, 50, 100, 200, or 400 ppm N. Destructive harvests were conducted six times at intervals from June through Dec. 1993. Leaf area, stem height, root length, root area, and dry weights of roots, stem, and leaves of harvested plants were measured and tissue nutrient concentrations were analyzed. There was no relationship between whole-plant N concentration and total plant biomass (r = 0). However, there were some linear relationships between total plant N and total plant biomass for an individual fertilizer treatment. Biomass allocation between root, stems, and leaves was very consistent across all fertilizer levels at any one harvest. Percent total N in roots, stems, and leaves also was fairly consistent across fertilizer levels. This was true at each harvest, except the first two, in which a greater percentage of total N was partitioned to the leaves and a smaller percentage was partitioned to the roots in the high (100, 200, 400 ppm N) fertilizer treatments. Whole-plant K concentrations increased with increasing fertilizer level, but decreased over time. Whole-plant P concentrations increased linearly with whole-plant dry weight in the higher (100, 200, 400 ppm N) fertilizer treatments.

Evaluation of early maturing cowpea (Vigna unguiculata) germplasm for variation in phosphorus use efficiency and biological nitrogen fixation potential with indigenous rhizobial populations

2016 ◽  
Vol 155 (1) ◽  
pp. 102-116 ◽  
Author(s):  
R. ABAIDOO ◽  
M. O. DARE ◽  
S. KILLANI ◽  
A. OPOKU
Keyword(s):  
Plant Biomass ◽  
Cropping Systems ◽  
High Capacity ◽  
Principal Component ◽  
N Fixation ◽  
Phosphorus Use Efficiency ◽  
Total N ◽  
Early Maturing ◽  
Use Efficiency ◽  
Biological Nitrogen

SUMMARYCowpea genotypes that efficiently utilize phosphorus (P) with high potential for biological nitrogen (N) fixation (BNF) are vital to sustainable cropping systems in West Africa. A total of 175 early maturing cowpea genotypes were evaluated in 2010 and 2011 for P use efficiency (PUE) and BNF with an indigenous rhizobial population at Shika in the Northern Guinea savanna of Nigeria. There were significant genotypic variations for all 11 variables measured. The P utilization index, percentage N derived from the atmosphere and total N fixed ranged between 2·10–4·67, 31·3–61·86% and 11·86–50 kg/ha, respectively. The 175 early maturing cowpea genotypes were divided into five categories using principal component analysis (PCA), whereby total N fixed was associated with N and P uptake and plant biomass yield. Complete linkage cluster analysis revealed a total of three distinctive clusters having remarkable correspondence with the PCA. Some genotypes were identified as potential candidates for further breeding programmes using high PUE genotypes with relatively high capacity for BNF and indigenous rhizobial populations.

scholarly journals Using Constructed Floating Wetlands to Remove Nutrients from a Waste Stabilization Pond

Water ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 1746
Author(s):  
Isaac Huth ◽  
Chris Walker ◽  
Ramraj Kulkarni ◽  
Terry Lucke
Keyword(s):  
Nutrient Uptake ◽  
Plant Species ◽  
Plant Tissue ◽  
Nutrient Concentration ◽  
Plant Biomass ◽  
Sewage Treatment ◽  
Biomass Accumulation ◽  
Uptake Rates ◽  
The Mean ◽  
Tissue Nutrient Concentration

This study reports the biomass accumulation, plant nutrient concentration, and nutrient uptake rates of plants in a constructed floating wetland (CFW) installed for a sewage treatment application in Australia. Plant biomass accumulation was estimated based on field samplings throughout the duration of the study. Analysis of samples of each plant species was also completed to estimate the mean plant tissue nutrient content. The plant biomass accumulation estimate and the mean plant tissue nutrient concentration were then used to estimate the total nutrient uptake for each species. Each of the species were found to differ in biomass accumulation and plant tissue nutrient concentration and the distribution of biomass and nutrients between the shoots and roots. The nutrient uptake rates varied between the species, with B. articulata having the greatest nutrient uptake rates (shoots: N, 104 ± 31.5 g/m2, P, 12.9 ± 3.87 g/m2; roots: N, 23.9 ± 7.23 g/m2, P, 5.54 ± 1.67 g/m2). Harvesting of the four CFW islands after 375 days of growth removed an estimated 23.2 kg of N and 2.97 kg of P. The results of this study indicate that the use of CFWs with carefully selected plant species can successfully remove significant amounts of nutrients from domestic wastewater.

scholarly journals Nitrogen Requirements of Drip-irrigated Processing Tomatoes

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1988-1993 ◽  
Author(s):  
Timothy K. Hartz ◽  
Thomas G. Bottoms
Keyword(s):  
N Uptake ◽  
N Fertilization ◽  
Fruit Yield ◽  
N Availability ◽  
Total N ◽  
Whole Plant ◽  
Biomass N ◽  
Highly Correlated ◽  
Whole Plants ◽  
Processing Tomatoes

As growers of processing tomato (Lycopersicon esculentum Mill.) adopt drip irrigation, plant vigor and fruit yield typically increase, suggesting a need for re-evaluation of established nitrogen (N) fertilization practices. Trials were conducted in California in 2007–2008 to evaluate growth and N uptake dynamics of drip-irrigated processing tomatoes across N fertigation regimes ranging from deficient to excessive. Whole plants were collected at 2-week intervals for determination of biomass and N content, recently matured whole leaves for total N and petioles for NO3-N. Additionally, six commercial fields were sampled at 3- to 4-week intervals to document N uptake and crop N status under conditions representative of the industry. A seasonal N rate of ≈200 kg·ha−1 appeared adequate to maximize fruit yield across the range of field conditions encountered. The four highest-yielding fields (143 Mg·ha−1 mean fresh fruit mass) averaged 14 Mg·ha−1 of above-ground biomass with fruit representing 62%; these fields averaged 296 kg·ha−1 biomass N, of which 71% was in fruit. The rate of biomass development and N uptake peaked during the period between early fruit setting and early red fruit development (a period of ≈6 weeks) during which N uptake averaged 4 to 5 kg·ha−1·d−1. Leaf N concentration was highly correlated with whole plant N (r2 = 0.83) and provided a reliable indicator of plant N sufficiency throughout the season. Petiole NO3-N did not reliably discriminate between crops with adequate or deficient N availability; current petiole NO3-N sufficiency guidelines are unrealistically high.

scholarly journals Diversity of Rhizobium leguminosarum from Pea Fields in Washington State

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Rita Abi-Ghanem ◽  
Jeffrey L. Smith ◽  
George J. Vandemark
Keyword(s):  
Genetic Diversity ◽  
Rhizobium Leguminosarum ◽  
Plant Biomass ◽  
Washington State ◽  
Yield Potential ◽  
Bootstrap Support ◽  
N Fixation ◽  
Plant Variety ◽  
Distinct Cluster ◽  
Biological Nitrogen

Rhizobia-mediated biological nitrogen (N) fixation in legumes contributes to yield potential in these crops and also provides residual fertilizer to subsequent cereals. Our objectives were to collect isolates of Rhizobium leguminosarum from several pea fields in Washington, examine genetic diversity among these isolates and several commercial isolates of R. leguminosarum, and compare genetically distinct isolates for their ability to fix N in a range of pea hosts. Seventy-nine isolates were collected from pea root from four noninoculated pea fields. Sequence-related amplified polymorphism (SRAP) markers generated by PCR were used to discriminate among isolates. Isolates fell into 17 clusters with robust bootstrap support values. Nearly half of the isolates fell into a single large cluster, but smaller clusters were also detected for isolates from all four field locations. The majority of commercial isolates fell into a distinct cluster. Four genetically distinct isolates were compared for their efficiency in fixing N in a greenhouse experiment. Host plant variety effects were significant for plant biomass due to N fixation and also for the quantity of N fixed per variety. Significant effects of R. leguminosarum isolates were observed for the quantity of N fixed per isolate, plant biomass, and the quantity of N per plant.

scholarly journals Effects of two wood-based biochars on the fate of added fertilizer nitrogen—a 15N tracing study

2021 ◽  
Author(s):  
Subin Kalu ◽  
Gboyega Nathaniel Oyekoya ◽  
Per Ambus ◽  
Priit Tammeorg ◽  
Asko Simojoki ◽  
...  
Keyword(s):  
Plant Uptake ◽  
Plant Biomass ◽  
Application Rate ◽  
Control Treatment ◽  
Organic N ◽  
N Leaching ◽  
Soil N ◽  
Mineral N ◽  
Total N ◽  
Application Rates

AbstractA 15N tracing pot experiment was conducted using two types of wood-based biochars: a regular biochar and a Kon-Tiki-produced nutrient-enriched biochar, at two application rates (1% and 5% (w/w)), in addition to a fertilizer only and a control treatment. Ryegrass was sown in pots, all of which except controls received 15N-labelled fertilizer as either 15NH4NO3 or NH415NO3. We quantified the effect of biochar application on soil N2O emissions, as well as the fate of fertilizer-derived ammonium (NH4+) and nitrate (NO3−) in terms of their leaching from the soil, uptake into plant biomass, and recovery in the soil. We found that application of biochars reduced soil mineral N leaching and N2O emissions. Similarly, the higher biochar application rate of 5% significantly increased aboveground ryegrass biomass yield. However, no differences in N2O emissions and ryegrass biomass yields were observed between regular and nutrient-enriched biochar treatments, although mineral N leaching tended to be lower in the nutrient-enriched biochar treatment than in the regular biochar treatment. The 15N analysis revealed that biochar application increased the plant uptake of added nitrate, but reduced the plant uptake of added ammonium compared to the fertilizer only treatment. Thus, the uptake of total N derived from added NH4NO3 fertilizer was not affected by the biochar addition, and cannot explain the increase in plant biomass in biochar treatments. Instead, the increased plant biomass at the higher biochar application rate was attributed to the enhanced uptake of N derived from soil. This suggests that the interactions between biochar and native soil organic N may be important determinants of the availability of soil N to plant growth.

scholarly journals Mangrove diversity enhances plant biomass production and carbon storage in Hainan island, China

2021 ◽  
Vol 35 (3) ◽  
pp. 774-786
Author(s):  
Jiankun Bai ◽  
Yuchen Meng ◽  
Ruikun Gou ◽  
Jiacheng Lyu ◽  
Zheng Dai ◽  
...  
Keyword(s):  
Carbon Storage ◽  
Biomass Production ◽  
Plant Biomass ◽  
Hainan Island ◽  
Plant Biomass Production

Can repeated soil amendment with biogas digestates increase soil suppressiveness toward non-specific soil-borne pathogens in agricultural lands?

2020 ◽  
pp. 1-12
Author(s):  
L. M. Manici ◽  
F. Caputo ◽  
G. A. Cappelli ◽  
E. Ceotto
Keyword(s):  
Plant Growth ◽  
Biomass Production ◽  
Soil Amendment ◽  
Plant Biomass ◽  
Amended Soils ◽  
Soil Suppressiveness ◽  
Soil Microbial ◽  
Soil Borne Pathogens ◽  
The Impact ◽  
Plant Biomass Production

Abstract Soil suppressiveness which is the natural ability of soil to support optimal plant growth and health is the resultant of multiple soil microbial components; which implies many difficulties when estimating this soil condition. Microbial benefits for plant health from repeated digestate applications were assessed in three experimental sites surrounding anaerobic biogas plants in an intensively cultivated area of northern Italy. A 2-yr trial was performed in 2017 and 2018 by performing an in-pot plant growth assay, using soil samples taken from two fields for each experimental site, of which one had been repeatedly amended with anaerobic biogas digestate and the other had not. These fields were similar in management and crop sequences (maize was the recurrent crop) for the last 10 yr. Plant growth response in the bioassay was expressed as plant biomass production, root colonization frequency by soil-borne fungi were estimated to evaluate the impact of soil-borne pathogens on plant growth, abundance of Pseudomonas and actinomycetes populations in rhizosphere were estimated as beneficial soil microbial indicators. Repeated soil amendment with digestate increased significantly soil capacity to support plant biomass production as compared to unamended control in both the years. Findings supported evidence that this increase was principally attributable to a higher natural ability of digestate-amended soils to reduce root infection by saprophytic soil-borne pathogens whose inoculum was increased by the recurrent maize cultivation. Pseudomonas and actinomycetes were always more abundant in digestate-amended soils suggesting that both these large bacterial groups were involved in the increase of their natural capacity to control soil-borne pathogens (soil suppressiveness).

scholarly journals Application of municipal biowaste derived products in Hibiscus cultivation: Effect on leaf gaseous exchange activity, and plant biomass accumulation and quality

2016 ◽  
Vol 205 ◽  
pp. 59-69 ◽  
Author(s):  
Daniele Massa ◽  
Domenico Prisa ◽  
Enzo Montoneri ◽  
Daniele Battaglini ◽  
Marco Ginepro ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Biomass Accumulation ◽  
Gaseous Exchange ◽  
Exchange Activity

scholarly journals The soil N cycle: new insights and key challenges

SOIL ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 235-256 ◽  
Author(s):  
J. W. van Groenigen ◽  
D. Huygens ◽  
P. Boeckx ◽  
Th. W. Kuyper ◽  
I. M. Lubbers ◽  
...  
Keyword(s):  
N Cycling ◽  
Greenhouse Gas Mitigation ◽  
Future Research ◽  
N Fixation ◽  
Gaseous Emissions ◽  
Soil N ◽  
Personal View ◽  
Total N ◽  
N Cycle ◽  
Soil N Cycle

Abstract. The study of soil N cycling processes has been, is, and will be at the centre of attention in soil science research. The importance of N as a nutrient for all biota; the ever-increasing rates of its anthropogenic input in terrestrial (agro)ecosystems; its resultant losses to the environment; and the complexity of the biological, physical, and chemical factors that regulate N cycling processes all contribute to the necessity of further understanding, measuring, and altering the soil N cycle. Here, we review important insights with respect to the soil N cycle that have been made over the last decade, and present a personal view on the key challenges of future research. We identify three key challenges with respect to basic N cycling processes producing gaseous emissions: 1. quantifying the importance of nitrifier denitrification and its main controlling factors; 2. characterizing the greenhouse gas mitigation potential and microbiological basis for N2O consumption; 3. characterizing hotspots and hot moments of denitrification Furthermore, we identified a key challenge with respect to modelling: 1. disentangling gross N transformation rates using advanced 15N / 18O tracing models Finally, we propose four key challenges related to how ecological interactions control N cycling processes: 1. linking functional diversity of soil fauna to N cycling processes beyond mineralization; 2. determining the functional relationship between root traits and soil N cycling; 3. characterizing the control that different types of mycorrhizal symbioses exert on N cycling; 4. quantifying the contribution of non-symbiotic pathways to total N fixation fluxes in natural systems We postulate that addressing these challenges will constitute a comprehensive research agenda with respect to the N cycle for the next decade. Such an agenda would help us to meet future challenges on food and energy security, biodiversity conservation, water and air quality, and climate stability.

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