Thermal Alkaline Method to Prepare Geotrichuam Candidum Protein Foaming Agent

2013 ◽  
Vol 641-642 ◽  
pp. 845-849
Author(s):  
Jie Jie Zheng ◽  
Ji Chuan Huo ◽  
Hong Lei ◽  
Wei Ai
Keyword(s):  
Mass Concentration ◽  
Geotrichum Candidum ◽  
Raw Material ◽  
Impact Factors ◽  
Optimization Strategy ◽  
Hydrolysis Time ◽  
Foam Expansion ◽  
Foaming Agent ◽  
Subsequent Response ◽  
Hydrolysis Temperature

Geotrichum Candidum strain numbered 2.0498 was used as raw material. The Geotrichum Candidum thallus were collected through inoculation and shaking cultivation. Thermal alkaline method was applied for hydrolyzing Geotrichum Candidum mycoprotein to prepare protein foaming agent. The effect of bacteria concentration, Ca(OH)2mass concentration, hydrolysis temperature and hydrolysis time on foam expansion were studied. The optimization strategy was single factor test and subsequent response surface analysis based on Box-Behnken design. The results showed that the optimized condition were a bacteria concentration of 5.4%(w/v) and a Ca(OH)2mass concentration of 1.5%(w/v), at a hydrolysis temperature of 95°C for 4.1 h. Under these optimal conditions, the foam expansion was 28.1. Compared with the model theory prediction, the relative error was 0.46%. At the same time, a multiple quadratic regression equation between Geotrichum Candidum mycoprotein foam expansion and impact factors was setted up and it played an important role in predicting the foam expansion.

Hydrolysis of S. platensis Using Sulfuric Acid for Ethanol Production

2022 ◽  
Vol 1048 ◽  
pp. 451-458
Author(s):  
Megawati ◽  
Astrilia Damayanti ◽  
Radenrara Dewi Artanti Putri ◽  
Zuhriyan Ash Shiddieqy Bahlawan ◽  
Astika Arum Dwi Mastuti ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Ethanol Production ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Second Step ◽  
Carbohydrate Composition ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Temperature ◽  
Hydrolysis Of

S. platensis is a microalga that contains carbohydrate composition of 30.21% which makes it potential to be used as raw material for ethanol production. Hydrolysis of S. platensis is the first step for converting its carbohydrates into monosaccharides. The second step is fermentation of monosaccharides into ethanol. This research aims to study the effect of temperature and microalgae concentration on the hydrolysis of S. platensis using sulfuric acid as catalyst. This research was conducted using 300 mL sulfuric acid of 2 mol/L, hydrolysis temperatures of 70, 80 and 90 °C, and microalgae concentrations of 20, 26.7, and 33.3 g/L. The effect of temperature is significant in the hydrolysis of S. platensis using sulfuric acid. At microalgae concentration of 20 g/L and hydrolysis time of 35 minutes, the higher the temperatures (70, 80, and 90 °C), the more the glucose yields would be (8.9, 13.5, and 22.9%). This temperature effect got stronger when the hydrolysis was running for 15 minutes. Every time the hydrolysis temperature increased by 10 °C, the glucose yield increased by 13.0% at microalgae concentration of 33.3 g/L. At temperature of 90 °C and time of 35 minutes, the higher the microalgae concentrations (20, 26.7, and 33.3 g/L), the higher the glucose yields would be (25.5, 27.7, and 28.2%). The highest glucose concentration obtained was 2.82 g/L at microalgae concentration of 33.3 g/L, temperature of 90 °C, and time of 35 minutes.

Technology Optimization on Preparation of Peanut Polypeptide Using Alcalase

2011 ◽  
Vol 236-238 ◽  
pp. 2586-2593 ◽  
Author(s):  
Li Na Yu ◽  
Qing Xuan Gong ◽  
Qing Li Yang ◽  
Jie Sun ◽  
Jie Bi ◽  
...  
Keyword(s):  
Influencing Factors ◽  
Mass Fraction ◽  
Mass Concentration ◽  
Ph Value ◽  
Protein Isolate ◽  
Peanut Protein ◽  
Soluble Nitrogen ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Enzyme Dosage

In order to exploit and utilize the peanut protein resource and produce high additional value protein products, the preparation technology of peanut polypeptide from peanut protein isolate was investigated using Alcalase. The effects of influencing factors, enzyme dosage, substrate mass fraction, hydrolysis temperature, hydrolysis time and pH value, on hydrolysis was studied during the process of Alcalase hydrolyzing peanut protein isolate by single factor experiment and response surface methodology central composite design. The regression model for the relationship between the influencing factors and the soluble nitrogen mass concentration was established. The optimum parameters for Alcalase enzymatic reaction were obtained as follows: pH value 8.0, enzyme dosage 4762U/g substrate, substrate mass fraction 5%, hydrolysis temperature 50°C, hydrolysis time 180min, and then the soluble nitrogen mass concentration reached to 16.28mg/mL.

scholarly journals Optimization and Quality Analysis of Different Extraction Methods of Palm Seed Oil

Complexity ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Qi-Zhao Li ◽  
Zheng-Qun Cai
Keyword(s):  
Enzymatic Hydrolysis ◽  
Seed Oil ◽  
Extraction Process ◽  
Extraction Methods ◽  
Raw Material ◽  
Quality Analysis ◽  
Enzymatic Extraction ◽  
Hydrolysis Time ◽  
Ultrasonic Assisted ◽  
Hydrolysis Temperature

The extraction process of palm seed oil was optimized. Using palm seed as raw material, oil extraction rate was used as an index. The effects of flash extraction, ultrasonic-assisted extraction, supercritical extraction, and aqueous enzymatic extraction on the yield of palm seed oil were investigated. The extraction methods and technological conditions of palm seed oil were optimized by the orthogonal method on the basis of single factor. The seed oil was analyzed and detected. The results showed that the water enzymatic extraction method was the best, and the optimal extraction conditions were as follows: enzymatic hydrolysis time 16 h, enzymatic hydrolysis temperature 50°C, and enzymatic content 2.0%. The oil yield of palm seed was 16.48%. Conclusion. Water enzymatic extraction process of palm seed oil is reasonable, the active ingredients are rich, and the quality of seed oil is better, providing reference for the development and research of palm seed oil.

USE OF THE MICROWAVE ENERGY FOR ALUMINUM WASTE FOAMING

2019 ◽  
Vol 25 (4) ◽  
pp. 43-49
Author(s):  
LUCIAN PAUNESCU ◽  
MARIUS FLORIN DRAGOESCU ◽  
SORIN MIRCEA AXINTE ◽  
ANA CASANDRA SEBE
Keyword(s):  
Thermal Conductivity ◽  
Compressive Strength ◽  
Porous Structure ◽  
Powder Mixture ◽  
Aluminum Foam ◽  
Fine Powder ◽  
Microwave Energy ◽  
Raw Material ◽  
Foaming Agent ◽  
Foaming Process

The paper presents an aluminum foam experimental technique using the microwave energy. The raw material was recycling aluminum waste processed by ecological melting and gas atomizing to obtain the fine powder required in the foaming process. The powder mixture was completed with dolomite as a foaming agent. The products had a fine and homogeneous porous structure (pore size between 0.4-0.9 mm). The density (1.17-1.19 g/cm3), the compressive strength (6.83-7.01 MPa) and the thermal conductivity (5.71-5.84 W/m·K) had values almost similar to the foams made by conventional methods.

Effect of Control Technology on Properties of Quartz Porous Brick

2018 ◽  
Vol 777 ◽  
pp. 564-568 ◽  
Author(s):  
Long He ◽  
Jin Shi Li ◽  
Mei Hua Chen ◽  
Yan Yang ◽  
Xin Peng Lou ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Quartz Sand ◽  
High Performance ◽  
Performance Test ◽  
Holding Time ◽  
Raw Material ◽  
Process Conditions ◽  
Low Grade ◽  
Sintering Process ◽  
Foaming Agent

A high-performance quartz sand insulation brick was prepared by using low grade quartz sand under different sintering process conditions. The optimum sintering process conditions were obtained by analyzing the relationship between microstructure and sintering process. Through the compounding, pulping, forming, drying and sintering processes, and the performance test of the porous brick, the following conclusions can be drawn, the comprehensive performance in all aspects, the porosity is similar, the preferred high compressive strength conditions, in order to get a best The bonding point, brick body sintering temperature of 1150 °C, porosity of 74.56%, compressive strength of 2.1 MPa of porous brick, and the pores are smooth, more uniform distribution. With the prolonging of the holding time, the porosity of the porous brick is reduced, and the performance is 1h, the porosity is 77.22% and the compressive strength is 2.05 MPa. When the raw material ratio is 60% quartz sand, 30wt% kaolin, calcium carbonate 9.6wt%, foaming agent 0.4wt%, water ratio 0.9 holding time at 1h sintering at 1150°C can get better porosity and compressive strength of the insulation brick. The porous material was sintered at 1150 °C, the content of foaming agent was 0.2wt%, the ratio of water to material was 0.9, and the compressive pressure and porosity were the better.

Research on the Performance of Plaster Material by Chemical Foaming Method

2013 ◽  
Vol 830 ◽  
pp. 435-438
Author(s):  
Hong Xia Wang ◽  
Bo Liu ◽  
Kuang Ping Yuan ◽  
Wu Biao Duan ◽  
Fei Hua Yang ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Flexural Strength ◽  
Water Temperature ◽  
Chemical Reaction ◽  
Raw Material ◽  
Foaming Agent ◽  
Cement Ratio ◽  
Water Cement Ratio ◽  
Chemical Foaming

The paper introduces a kind of foaming plaster with main raw material of α- gypsum, which is produced by utilizing the chemical reaction principles H2O2 was decomposed. Meanwhile，study the changes of density，flexural strength and compressive strength of plaster material performance with the additives in different ratio. The result showed that water-cement ratio, H2O2 foaming agent and water temperature all affect the performance of the foaming plaster.

scholarly journals Bioethanol production from mixed culture microalgae biomass with temperature hydrolysis variation

2018 ◽  
Vol 197 ◽  
pp. 13010 ◽  
Author(s):  
I Gede Aditya Juliarnita ◽  
Rositayanti Hadisoebroto ◽  
Astri Rinanti
Keyword(s):  
Mixed Culture ◽  
Alternative Fuels ◽  
Color Change ◽  
Scenedesmus Obliquus ◽  
Raw Material ◽  
Microalgae Biomass ◽  
Hydrolysis Temperature ◽  
Last Stage ◽  
Preliminary Study ◽  
Carbohydrate Levels

This preliminary study aims to exploit the biomass of microalgae Chlorella vulgaris; Scenedesmus obliquus; and Chlorococcum sp. in the form of a mixed culture as raw material of alternative fuels. Microalgae were cultivated in the artificial growth medium of PHM (Provasoli Haematococcus Media) for 9 days to reach the exponential phase. Hydrolysis was carried out at a temperature variation of (oC) 25; 80; 100; 120; 140; 160 within 30 minutes by adding hydrochloric acid. Biomass fermentation by adding 50% (v/v) Saccharomyces cerevisiae for 5 day to produce alcohol compounds. The last stage is separation of the alcohol compounds from another compounds by distillation. The result showed that carbohydrate levels with color change indicator in luff schoorl solution at hydrolysis and fermentation stages severely were 12.20 mg/L. Carbohydrate levels in fermentation stage produced 17% (v/v) alcohol compounds while in the distillation stage, 98% (v/v) alcohol will be separated into intermediate compounds such as linoleic acid and methyl alcohol as a characteristic of bioethanol. At 80oC of hydrolysis temperature, 58% bioethanol was produced. Further research is needed, since the preliminary study proves the mixed culture of microalgae is potentially to be utilized in producing bioethanol.

Study on the BTSPS Silane Film Prepared on the Surface of the 2A12 Aluminum Alloy

2014 ◽  
Vol 936 ◽  
pp. 1071-1076
Author(s):  
Long Jiang Peng ◽  
Shao Hui Chen ◽  
Ying Ning Feng ◽  
Tian Yi Li
Keyword(s):  
Aluminum Alloy ◽  
Volume Fraction ◽  
Orthogonal Experiment ◽  
Influence Factors ◽  
Dip Coating ◽  
Hydrolysis Time ◽  
Electrochemical Test ◽  
Coating Method ◽  
Hydrolysis Temperature ◽  
Dip Coating Method

The protective silane film BTSPS was prepared on the surface of 2A12 aluminum alloys by dip-coating method in this paper. The influence factors such as the volume fraction, hydrolysis time, hydrolysis temperature and dip-coating time of BTSPS were studied through orthogonal experiment. The optimum conditions is: the volume fraction is 5%; hydrolysis time is 180min; Hydrolysis temperature is 20°C; Dipping time is 10 min. The silane film performance was investigated by electrochemical test, sodium chloride solution immersion and scanning electron microscope (SEM). The results show that the corrosion resistance of the 2A12 aluminum alloy coated with BTSPS is improved greatly.

scholarly journals Effect of Temperature, pH, Enzyme to Substrate Ratio, Substrate Concentration and Time on the Antioxidative Activity of Hydrolysates from Goat Milk Casein by Alcalase

2016 ◽  
Vol 20 (2) ◽  
pp. 29-38 ◽  
Author(s):  
Guowei Shu ◽  
Bowen Zhang ◽  
Qian Zhang ◽  
Hongchang Wan ◽  
Hong Li
Keyword(s):  
Substrate Concentration ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Goat Milk ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Milk Casein

Abstract The effect of hydrolysis temperature (45, 50, 55, 60 and 65°C), pH (7.0, 7.5, 8.0, 8.5 and 9.0), enzyme to substrate (E/S) ratio (1.0, 1.5, 2.0, 2.5 and 3.0%), substrate concentration (2, 3, 4, 5 and 6%) and hydrolysis time (30-240min) on antioxidant peptides hydrolysated from goat’s milk casein by Alcalase was investigated using single factor experiment. In order to obtain high DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity, the optimal conditions were hydrolysis time of 150 min, temperature of 50°C, pH 8.0, E/S ratio of 2.0% and substrate concentration of 4.0%. The hydrolysis time, hydrolysis temperature, pH, E/S ratio and substrate concentration had a significant influence on degree of hydrolysis, metal-chelating activity, DPPH and superoxide radical scavenging activity on casein hydrolysate of goat milk by Alcalase, the results were beneficial for further provide theoretical basis for production of antioxidant peptides.

Research on Influencing Factors of Wood Residual Fiber Foaming Cushion Material

2012 ◽  
Vol 511 ◽  
pp. 46-50
Author(s):  
Bin Cao ◽  
Sheng Ling Xiao ◽  
Xiao Qing Pan
Keyword(s):  
Mechanical Property ◽  
Surface Quality ◽  
Influencing Factors ◽  
Compression Strength ◽  
Wood Fiber ◽  
Compression Modulus ◽  
Hot Compression ◽  
Raw Material ◽  
Single Factor ◽  
Foaming Agent

A wood residual fiber foaming cushion material was prepared by using gathered wood fiber as main raw material, starch/PVA adhesive as matrix, adding a certain amount of foaming agent and related additives, hot compression molding to shape up. This paper makes an investigation of single factor on the product’s surface quality and related mechanical property. Results show that with the amount of wood residual fiber increases, the rebound resilience of the product first increases then decreases. The density decreases along with the amount of foaming agent increases. The compression strength and compression modulus of the product increases along with the amount of wood residual fiber increases, it decreases along with the amount of foaming agent increases.

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