Study on Extracting TB from Liubao Tea with Cellulase Auxiliary Method

2013 ◽  
Vol 781-784 ◽  
pp. 1870-1874
Author(s):  
Ying Zi He ◽  
Xiao Wang ◽  
Xue Hong Zhang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Influence Factors ◽  
Orthogonal Test ◽  
Test Results ◽  
Single Factor ◽  
Extraction Condition ◽  
Hydrolysis Time ◽  
Orthogonal Experiments ◽  
Hydrolysis Temperature

A new way using cellulase as auxiliary method to extract theabrownin (TB) from Guangxi Liubao Tea was proposed in this article. Single factor and orthogonal experiments were used to analysis the best extraction condition of TB from Tea. The effects of the enzyme dose, enzymatic hydrolysis time, enzymatic hydrolysis temperature and the PH of extraction were investigated by orthogonal test. Results showed that the order of influence factors was enzymatic hydrolysis time, the PH of extraction, enzyme dose and enzymatic hydrolysis temperature. The optimal extraction conditions were determined as follows: the dosage of cellulose 20 mg/g, the enzymatic hydrolysis time 40 min, the enzymatic hydrolysis temperature 30°C,the extraction PH=4. The highest extraction ration can attach 24.503%.

Optimization of Extraction Conditions of Essential Oil from Latifolia miq Roots and Stems with Supercritical CO2 Fluid

2013 ◽  
Vol 726-731 ◽  
pp. 4512-4516
Author(s):  
Ren Yong Gu ◽  
Zhi Ping Li
Keyword(s):  
Essential Oil ◽  
Optimum Condition ◽  
Raw Materials ◽  
Extraction Process ◽  
Orthogonal Test ◽  
Single Factor ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

Studying on the extraction process of essential oil from Latifolia Miq roots and stems by Supercritical CO2 extraction. The effects on extracting ratio were investigated by using single factor test, adding content of grinding fineness of raw materials,extracting temperature, extracting pressure and extracting time. The optimum extraction condition was determined by orthogonal test. The results showed that the optimum condition of the extraction was as follows: grinding fineness 90 mesh, extracting temperature 45 °C, extracting pressure 20 MPa and extracting time 2.5 h.

Study on the BTSPS Silane Film Prepared on the Surface of the 2A12 Aluminum Alloy

2014 ◽  
Vol 936 ◽  
pp. 1071-1076
Author(s):  
Long Jiang Peng ◽  
Shao Hui Chen ◽  
Ying Ning Feng ◽  
Tian Yi Li
Keyword(s):  
Aluminum Alloy ◽  
Volume Fraction ◽  
Orthogonal Experiment ◽  
Influence Factors ◽  
Dip Coating ◽  
Hydrolysis Time ◽  
Electrochemical Test ◽  
Coating Method ◽  
Hydrolysis Temperature ◽  
Dip Coating Method

The protective silane film BTSPS was prepared on the surface of 2A12 aluminum alloys by dip-coating method in this paper. The influence factors such as the volume fraction, hydrolysis time, hydrolysis temperature and dip-coating time of BTSPS were studied through orthogonal experiment. The optimum conditions is: the volume fraction is 5%; hydrolysis time is 180min; Hydrolysis temperature is 20°C; Dipping time is 10 min. The silane film performance was investigated by electrochemical test, sodium chloride solution immersion and scanning electron microscope (SEM). The results show that the corrosion resistance of the 2A12 aluminum alloy coated with BTSPS is improved greatly.

Preparation Technology of Hydrolysis Protein of Tilapia Meat

2012 ◽  
Vol 550-553 ◽  
pp. 1160-1167
Author(s):  
Xi Juan Fang ◽  
Lu Xin Chen ◽  
Qing Zhu Zeng ◽  
Qing Ling Xu ◽  
Yu Zhan ◽  
...  
Keyword(s):  
Regression Model ◽  
Response Surface ◽  
Small Deviation ◽  
Degree Of Hydrolysis ◽  
Soluble Nitrogen ◽  
Single Factor ◽  
Hydrolysis Time ◽  
Preparation Technology ◽  
Hydrolysis Temperature ◽  
Acid Solubility

The preparation technology of tilapia protein hydrolysates by building single factor test and response surface methodology (RSM) were defined. The effect of hydrolysis temperature, pH, the additive quantity of Alcalase, the ratio of liquid-to-solid on degree of hydrolysis (DH), TCA-soluble nitrogen index (TCA-NSI) and yield of acid-solubility peptide (YASP) by regression model was obtained by response surface analysis. Regression model indicated that the optimal condition hydrolyzed animal protein of tilapia meat by proteinase was described as following：hydrolysis temperature 54.30°C, pH 8.77, the additive quantity of Alcalase 2.4L was 3702U/g, the ratio of liquid-to-solid was 3.06, hydrolysis time 1 hour. Under such conditions, the DH(%), TCA-NSI(%) and YASP(%) reached 33.63%, 22.10%, 64.55%, respectively. Experiment result showed that the predictive value and experimental value was small deviation and indicated that regression model was reasonable and reliable.

Preparation of Denatured Alcohol-Modified SPI-Based Adhesive

2012 ◽  
Vol 557-559 ◽  
pp. 987-990
Author(s):  
Yu Feng Ban ◽  
Hai Feng Zhu ◽  
Wei Zhao
Keyword(s):  
Shear Strength ◽  
Calcium Hydroxide ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Orthogonal Test ◽  
Test Results ◽  
Single Factor ◽  
Air Drying ◽  
Environment Friendly

An environment-friendly soy protein isolate (SPI)-based adhesive for wood was prepared using denatured alcohol-modified SPI, calcium hydroxide and sodium silicate. On the basis of the single factor test results, an orthogonal test of 4 elements and 3 levels was carried out to optimize formula ingredients. The modified SPI adhesive contained more calcium hydroxide than unmodified SPI adhesive and exhibited good performance. The shear strength of the specimen bonded with the modified adhesive was 82.3 MPa; after three cycles of water-soaking and air-drying, the decrease in shear strength was as low as 7.9%.

scholarly journals Hydrolysate from Mussel Mytilus galloprovincialis Meat: Enzymatic Hydrolysis, Optimization and Bioactive Properties

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5228
Author(s):  
Sara A. Cunha ◽  
Rita de Castro ◽  
Ezequiel R. Coscueta ◽  
Manuela Pintado
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Mytilus Galloprovincialis ◽  
Target Market ◽  
Hydrolysis Time ◽  
Bioactive Properties ◽  
Hydrolysis Temperature ◽  
Market Criteria ◽  
Enzyme Ratio ◽  
Production Conditions

Mussel production generates losses and waste since their commercialisation must be aligned with target market criteria. Since mussels are rich in proteins, their meat can be explored as a source of bioactive hydrolysates. Thus, the main objective of this study was to establish the optimal production conditions through two Box–Behnken designs to produce, by enzymatic hydrolysis (using subtilisin and corolase), hydrolysates rich in proteins and with bioactive properties. The factorial design allowed for the evaluation of the effects of three factors (hydrolysis temperature, enzyme ratio, and hydrolysis time) on protein/peptides release as well as antioxidant and anti-hypertensive properties of the hydrolysates. The hydrolysates produced using the optimised conditions using the subtilisin protease showed 45.0 ± 0.38% of protein, antioxidant activity via ORAC method of 485.63 ± 60.65 µmol TE/g of hydrolysate, and an IC50 for the inhibition of ACE of 1.0 ± 0.56 mg of protein/mL. The hydrolysates produced using corolase showed 46.35 ± 1.12% of protein, antioxidant activity of 389.48 ± 0.21 µmol TE/g of hydrolysate, and an IC50 for the inhibition of ACE of 3.7 ± 0.33 mg of protein/mL. Mussel meat losses and waste can be used as a source of hydrolysates rich in peptides with relevant bioactive properties, and showing potential for use as ingredients in different industries, such as food and cosmetics, contributing to a circular economy and reducing world waste.

Effects of the Protein in Wheat Straw on Enzymatic Hydrolysis

2013 ◽  
Vol 690-693 ◽  
pp. 1198-1202 ◽  
Author(s):  
Hui Chen ◽  
Lian Jie Wang ◽  
Tao Zhang ◽  
Meng Yu ◽  
Xin Ming Wang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Degradation Rate ◽  
Cellulose Degradation ◽  
Hydrolysis Time ◽  
Reaction Conditions ◽  
Orthogonal Experiments ◽  
Enzyme Dosage ◽  
Solid Liquid ◽  
Hydrolysis Of

The article used acid protease to remove the protein in wheat straw, and investigated the influence of wheat straw without the protein to enzymatic hydrolysis. By the single factor experiments, the cellulose degradation rate of wheat straw removed the protein was significantly higher than untreated, in the same conditions of enzymatic hydrolysis. It is shortening 24h of enzymatic hydrolysis time and reducing enzyme dosage 90mg/g. By the orthogonal experiments, the optimal reaction conditions for enzymatic hydrolysis of wheat straw with protein removed is the cellulase dosage 170mg, enzymatic time 56h, solid-liquid ratio 1:20, pH 4.8, the reaction temperature 50°C. Compared to the untreated, the cellulose degradation rate increased by 35.58%.

Evaluation and Optimization of The Factors Affectiing Enzymatic Hydrolysis of Silk Fibroin

2011 ◽  
Vol 399-401 ◽  
pp. 1536-1539
Author(s):  
Lei Zhong ◽  
Bing Lu ◽  
An Ping Liao
Keyword(s):  
Enzymatic Hydrolysis ◽  
Silk Fibroin ◽  
Ph Value ◽  
Enzyme Concentration ◽  
Single Factor ◽  
Orthogonal Experiments ◽  
Hydrolysis Rates ◽  
Enzyme Molecules ◽  
Optimized Conditions ◽  
Hydrolysis Of

Enzymatic hydrolysis of silk fibroin is studied in detail by both single factor experiments and orthogonal experiments. The results of single factor experiments show that enzyme concentration, temperature and pH value have significant effects on the hydrolysis. With the changes of them, the structures of enzyme molecules will also change, which will cause different hydrolysis rates. According to the results of orthogonal experiments, the degree of influence of these three factors can be sorted in descending order: temperature, concentration and then pH value. The optimized conditions of hydrolysis achieved in this paper are enzyme concentration 0.5g/L, temperature 60°C and pH value 8.60.

Application of Modified Nano TiO2 in the Binder of Artificial Marble

2015 ◽  
Vol 727-728 ◽  
pp. 154-157
Author(s):  
Min Yang ◽  
Ling Pan ◽  
Zhi Qiang Fan
Keyword(s):  
Compressive Strength ◽  
Chemical Modification ◽  
Coupling Agent ◽  
Influence Factors ◽  
Orthogonal Test ◽  
Condensation Temperature ◽  
Comprehensive Performance ◽  
Hydrolysis Temperature ◽  
Main Influence ◽  
Research Studies

The research studies the chemical modification of nano TiO2 and its application in the preparation of binder of artificial marble with superior comprehensive performance. Considering the content of coupling agent(KH-570), modification time, hydrolysis temperature and condensation temperature as the main influence factors, through orthogonal test designs, the optimal modification parameters were obtained. The results show that the hydrophobic of modified nanoTiO2 can reache 47%, and the artificial marble using polyster binder with modified nono TiO2 as binder obtains compressive strength as high as about 100 MPa.

scholarly journals TEST RESEARCH ON THE IMPACT PEAK FORCE AND DAMAGE DEPTH OF POTATO

2020 ◽  
Vol 61 (2) ◽  
pp. 105-114
Author(s):  
Weigang Deng ◽  
Chunguang Wang ◽  
Shengshi Xie
Keyword(s):  
Influence Factors ◽  
Orthogonal Test ◽  
Peak Force ◽  
Drop Height ◽  
Single Factor ◽  
Potato Varieties ◽  
Impact Peak ◽  
Damage Depth ◽  
The Impact

To analyse the influence factors of impact peak force (IPF) and damage depth (DD) on potatoes, the orthogonal test and single factor test were carried out on two potato varieties. The results showed that the IPF of Xiabodi was smaller and DD was greater than those of Gaoyuanhong. Potato mass had the greatest effect on IPF, and that of drop height on DD. The equations between IPF, DD and potato mass, drop height were obtained. Both IPF and DD impacting with steel were the largest, and the smallest with steel- rubber. With the increase of impact times, IPF decreased first and then increased, and DD increased gradually.

scholarly journals Enzymatic Hydrolysis of Lignocellulose for Bioethanol Production

2017 ◽  
Vol 71 (4) ◽  
pp. 275-279
Author(s):  
Linda Rozenfelde ◽  
Māris Puķe ◽  
Irēna Krūma ◽  
Ieva Poppele ◽  
Nataļja Matjuškova ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Effect Of Temperature ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Temperature ◽  
Different Temperatures ◽  
C 50 ◽  
Hydrolysis Of ◽  
Temperature Time ◽  
Furfural Production

Abstract The effect of temperature, time and amount of enzyme on hydrolysis of wheat straw lignocellulose remaining after furfural production was studied. The residual substrate was subjected to enzymatic hydrolysis at different temperatures — 45 °C, 50 °C and 55 °C. Hydrolysis time was 72 hours, and samples were taken every 24 hours. The maximum glucose yield (76.5% of the theoretically possible) was reached when hydrolysis temperature 50 °C was used. The production rate of glucose increased with a hydrolysis period of time. The yield of glucose significantly depended on the ratio of enzyme to substrate.

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