Study on Preparation of Star PLGA Cucurbitacin-Loaded Microsphere and its Propertities

This study used Ag-embedded nanoTiO2, xylan and water-soluble Chitosan as antibacterial agents, respectively prepared shutter blades through the treating solution of the different concentration and the different drug loading amount of the poplar veneer. Through a single factor experiment, this paper analyzes that the different antibacterial agent, concentration of antibacterial agent and the drug loading amount have an effect on the antibacterial properties of the shutter blades. The results show that the order of antibacterial performance of the shutter blades impregnated antibacterial agents is the Ag-embedded nanoTiO2, Chitosan, Xylan. Comprehensiv-ely thought the antibacterial properties and economic index, the optimal concentration of the Ag-embedded nanoTiO2 impregnation solution is 1%.

Download Full-text

The properties of MOF-Zn2(EBNB)2(BPY)2·2H2O and its basic study of loading Methadone

10.21203/rs.3.rs-42650/v4 ◽

2020 ◽

Author(s):

Deng Linxin ◽

Li Song ◽

Xuehua Sun

Keyword(s):

Drug Loading ◽

In Vitro Release ◽

In Vitro Cytotoxicity ◽

Cytotoxicity Test ◽

Two Phase ◽

Basic Study ◽

Nitrobenzoic Acid ◽

Model Drug ◽

Loading Amount

Abstract The ligands of (E)-bis(p-3-nitrobenzoic acid) vinyl (C16H10N2O8) were synthesized in three steps, and then the MOF-Zn2(EBNB)2(BPY)2·2H2O was synthesized by solvothermal method. This structure was characterized by X-ray single crystal diffraction, SEM and TG. The drug loading and in vitro release of Zn2(EBNB)2(BPY)2·2H2O were also studied with Methadone as model drug. The result shows that the highest loading amount of Zn2(EBNB)2(BPY)2·2H2O to Methadone was 0.256g/g, and the drug delivery system was a two-phase mode. The results of in vitro cytotoxicity test shows that Zn2(EBNB)2(BPY)2·2H2O has good biocompatibility.

Download Full-text

Characterization of Drugs with Good Glass Formers in Loaded-Mesoporous Silica and Its Theoretical Value Relevance with Mesopores Surface and Pore-Filling Capacity

Pharmaceuticals ◽

10.3390/ph15010093 ◽

2022 ◽

Vol 15 (1) ◽

pp. 93

Author(s):

Arif Budiman ◽

Diah Lia Aulifa

Keyword(s):

Mesoporous Silica ◽

Silica Surface ◽

Value Relevance ◽

Drug Loading ◽

Surface Interaction ◽

Pore Filling ◽

Maximum Loading ◽

Loading Amount ◽

Theoretical Amount

The incorporation of a drug into mesoporous silica (MPS) is a promising strategy to stabilize its amorphous form. However, the drug within MPS has shown incomplete release, despite a supersaturated solution being generated. This indicates the determination of maximum drug loading in MPS below what is experimentally necessary to maximize the drug doses in the system. Therefore, this study aimed to characterize the drugs with good glass former loaded-mesoporous silica, determine the maximum drug loading, and compare its theoretical value relevance to monolayer covering the mesoporous (MCM) surface, as well as pore-filling capacity (PFC). Solvent evaporation and melt methods were used to load each drug into MPS. In addition, the glass transition of ritonavir (RTV) and cyclosporine A (CYP), as well as the melting peak of indomethacin (IDM) and saccharin (SAC) in mesoporous silica, were not discovered in the modulated differential scanning calorimetry (MDSC) curve, demonstrating that each drug was successfully incorporated into the mesopores. The amorphization of RTV-loaded MPS (RTV/MPS), CYP-loaded MPS (CYP/MPS), and IDM-loaded MPS (IDM/MPS) were confirmed as a halo pattern in powder X-ray diffraction measurements and a single glass transition event in the MDSC curve. Additionally, the good glass formers, nanoconfinement effect of MPS and silica surface interaction contributed to the amorphization of RTV, CYP and IDM within MPS. Meanwhile, the crystallization of SAC was observed in SAC-loaded MPS (SAC/MPS) due to its weak silica surface interaction and high recrystallization tendency. The maximum loading amount of RTV/MPS was experimentally close to the theoretical amount of MCM, showing monomolecular adsorption of RTV on the silica surface. On the other hand, the maximum loading amount of CYP/MPS and IDM/MPS was experimentally lower than the theoretical amount of MCM due to the lack of surface interaction. However, neither CYP or IDM occupied the entire silica surface, even though some drugs were adsorbed on the MPS surface. Moreover, the maximum loading amount of SAC/MPS was experimentally close to the theoretical amount of PFC, suggesting the multilayers of SAC within the MPS. Therefore, this study demonstrates that the characterization of drugs within MPS, such as molecular size and interaction of drug-silica surface, affects the loading efficiency of drugs within MPS that influence its relevance with the theoretical value of drugs.

Download Full-text

The properties of MOF-Zn2(EBNB)2(BPY)2·2H2O and its basic study of loading Methadone

10.21203/rs.3.rs-42650/v3 ◽

2020 ◽

Author(s):

Deng Linxin ◽

Li Song ◽

Xuehua Sun

Keyword(s):

Drug Loading ◽

In Vitro Release ◽

In Vitro Cytotoxicity ◽

Cytotoxicity Test ◽

Two Phase ◽

Basic Study ◽

Nitrobenzoic Acid ◽

Model Drug ◽

Loading Amount

Abstract The ligands of (E)-bis(p-3-nitrobenzoic acid) vinyl (C16H10N2O8) were synthesized in three steps, and then the MOF-Zn2(EBNB)2(BPY)2·2H2O was synthesized by solvothermal method. This structure was characterized by X-ray single crystal diffraction, SEM and TG. The drug loading and in vitro release of Zn2(EBNB)2(BPY)2·2H2O were also studied with Methadone as model drug. The result shows that the highest loading amount of Zn2(EBNB)2(BPY)2·2H2O to Methadone was 0.256g/g, and the drug delivery system was a two-phase mode. The results of in vitro cytotoxicity test shows that Zn2(EBNB)2(BPY)2·2H2O has good biocompatibility.

Download Full-text

Synthesis and thermal stability of star polymer with eight arms using polyhedral oligomeric silsesquioxane as a core

e-Polymers ◽

10.1515/epoly.2010.10.1.1587 ◽

2010 ◽

Vol 10 (1) ◽

Author(s):

Lei Liu ◽

Wenping Wang ◽

Xianran Guo

Keyword(s):

Thermal Stability ◽

Polyhedral Oligomeric Silsesquioxane ◽

Catalyst System ◽

Star Polymer ◽

Good Thermal Stability ◽

Methyl Methacrylates ◽

Oligomeric Silsesquioxane ◽

Polymerization Method ◽

Form Star ◽

Thermal Stability Of

AbstractA Polyhedral oligomeric silsesquioxane (POSS) containing eight 3- chloropropyl groups was synthesized and incorporated into poly(methyl methacrylates) to form star polymer. First, octa(3-chloropropyl) POSS [(ClCH2CH2CH2)8Si8O12] was prepared by hydrolysis and condensation of 3- chloropropyltriethoxysilane, and then two star-shaped POSS/PMMA composites with different POSS content was synthesized at 110 °C, using octa(3-chloropropyl) POSS as an multifunctional initiator, CuCl and 2-2’ bipyridine (Bpy) as catalyst system. The structures of octa(3-chloropropyl) POSS and star-shaped POSS/PMMA composite were characterized by analytical methods including FTIR, NMR, GPC and XRD, with results showing that POSS molecule was successfully formed which accorded well with the reported work and was dispersed into PMMA matrix evenly by atom transfer radical polymerization method. The TGA results indicated that synthetic POSS molecule possessed good thermal stability, and the thermal stability of POSS/PMMA composite enhanced with the increase of POSS content, which is due to the incorporation of inorganic POSS molecule

Download Full-text

Synthesis and Characterization of Starch-Graft-Acrylamide Hydrogel for Oral Drug Delivery

Nigerian Journal of Basic and Applied Sciences ◽

10.4314/njbas.v27i2.3 ◽

2020 ◽

Vol 27 (2) ◽

pp. 16-21

Author(s):

H. Musa ◽

Y. Musa ◽

M. Suleiman

Keyword(s):

Drug Delivery ◽

Oral Drug Delivery ◽

Drug Loading ◽

Oral Drug ◽

Intestinal Fluid ◽

Simulated Intestinal Fluid ◽

Swelling Capacity ◽

Model Drug ◽

Polymerization Method

In this research, starch was extracted from fresh sweet potato and was used to prepare starch-g-acrylamide hydrogel using free radical polymerization method with potassium per sulphate and N’N-Methylene bisacrylamide as initiator and cross-linker, respectively. The swelling capacity and pH sensitivity of the synthesized hydrogel were investigated in solutions of various pH (1-12). The drug loading and release experiment was also carried out using promethazine (PMZ) as the model drug at 25oC and 37oC, respectively while the release study was carried out in an enzyme-free simulated gastric intestinal fluid (SGF) and simulated intestinal fluid (SIF). The result showed a 905% swelling at pH 11, suggesting increased swelling capacity at higher pH values. Drug loading result indicated 99% of the drug was entrapped by the hydrogel as confirmed by UV-visible spectrophotometry. SIF and SGF Simulation indicated a 24% and 9% drug release for the first ten hours. At the end of 48 hours the release was 96% and 89%, respectively indicating the hydrogel released more promethazine in SIF than in SGF. The results obtained in this work suggest that starch-graft-acrylamide hydrogel is a potential vehicle for oral drug delivery. Keywords: Starch, Acrylamide, Hydrogel, Drug delivery.

Download Full-text

Biodegradable Redox-Sensitive Star Polymer Nanomicelles for Enhancing Doxorubicin Delivery

Nanomaterials ◽

10.3390/nano9040547 ◽

2019 ◽

Vol 9 (4) ◽

pp. 547 ◽

Cited By ~ 3

Author(s):

Meng Li ◽

Jian-Wei Guo ◽

Wei-Qiu Wen ◽

Jem-Kun Chen

Keyword(s):

Therapeutic Potential ◽

Drug Loading ◽

Monomethyl Ether ◽

Star Polymer ◽

Anti Cancer ◽

Good Biocompatibility ◽

Delivery Vehicles ◽

Poly Ethylene ◽

Biological Environment ◽

Esterification Reactions

A typical amphiphilic star polymer adamantane-[poly(lactic-co-glycolic acid)-bis(2-carboxyethyl) sulfide-poly(ethylene glycol) monomethyl ether)]4 with a specific hydrophilic/redox-sensitive/hydrophobic structure was designed and synthesized through ring opening and esterification reactions. The self-assembled nanomicelles were used as doxorubicin (DOX) delivery vehicles with suitable critical micelle concentrations (5.0 mg/L). After the drug being loaded, drug-loaded micelles showed good drug-loading efficiency (10.39%), encapsulation efficiency (58.1%), and drug release (up to 60%) under simulated biological environment conditions. In addition, the backbone structure of the biodegradable polymer was easily hydrolyzed by the action of biological enzymes. As expected, cell-based studies showed that the designed polymer micelles possessed good biocompatibility (a survival rate of 85% for NH-3T3 cells). Moreover, the drug (DOX) still maintained good anti-cancer effects after being loaded, which caused 40% of MCF-7 cells to survive. These redox-sensitive micelles showed anti-tumor therapeutic potential.

Download Full-text

Preparation and Characterization of Silk Fibroin Microspheres

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.175-176.110 ◽

2011 ◽

Vol 175-176 ◽

pp. 110-115 ◽

Cited By ~ 1

Author(s):

Rui Juan Xie ◽

Hai Yan Wu ◽

Mei Nan Zhu ◽

Yang Yang Huang

Keyword(s):

Silk Fibroin ◽

Drug Loading ◽

Liquid Paraffin ◽

Particle Diameter ◽

Water Phase ◽

Burst Release ◽

Continuous Release ◽

Loading Efficiency ◽

Drug Loading Efficiency ◽

Loading Amount

An improved method of water-in-oil-in-water (w1/o/w2) multi-emulsion was developed to prepare silk fibroin (SF) microspheres that had improved drug-loading amount and better drug-loading efficiency. SF and dexamethasone sodium phosphate (DSP) were used as one water phase(w1), liquid paraffin was used as oil phase and isopropanol was used as the other water phase(w2). In the preparation process, isopropanol was also used to induce the crystallization of SF. The SF microsphere was mainly composed of silk II proteins. The drug-loading amount of SF microsphere ranged from 28.45 µg/mg to 79.21 µg/mg, and the drug-loading efficiency varied from 30.80% to 87.16%. The spherical morphology of the microsphere was observed by SEM. The particle diameter was measured by the Laser particle sizer and their average sizes varied from 7.41 µm to 66.10 µm. The drug releasing profile of the microspheres was measured in vitro. The burst release was significant when isopropanol-to-SF ratio was lower than 3:1，although the drug-loading amount and the drug-loading efficiency was high; It showed a higher drug-loading amount and efficiency, as well as an obvious continuous release effect when the ratio was higher than 4:1; It also showed a certain continuous release effect when the ratio was 4:1.

Download Full-text

Chitosan overlaid Fe3O4/rGO nanocomposite for targeted drug delivery, imaging, and biomedical applications

Scientific Reports ◽

10.1038/s41598-020-76015-3 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Viswanathan Karthika ◽

Mohamad S. AlSalhi ◽

Sandhanasamy Devanesan ◽

Kasi Gopinath ◽

Ayyakannu Arumugam ◽

...

Keyword(s):

Drug Delivery ◽

Folic Acid ◽

Targeted Drug Delivery ◽

Drug Carrier ◽

Drug Loading ◽

Antioxidant Properties ◽

Targeted Drug ◽

Loading Amount

Abstract A hybrid and straightforward nanosystem that can be used simultaneously for cancer-targeted fluorescence imaging and targeted drug delivery in vitro was reported in this study. A chitosan (CS) polymer coated with reduced graphene oxide (rGO) and implanted with Fe3O4 nanoparticles was fabricated. The fundamental physicochemical properties were confirmed via FT-IR, XRD, FE-SEM, HR-TEM, XPS, and VSM analysis. The in vivo toxicity study in zebrafish showed that the nanocomposite was not toxic. The in vitro drug loading amount was 0.448 mg/mL−1 for doxorubicin, an anticancer therapeutic, in the rGO/Fe3O4/CS nanocomposite. Furthermore, the pH-regulated release was observed using folic acid. Cellular uptake and multimodal imaging revealed the benefit of the folic acid-conjugated nanocomposite as a drug carrier, which remarkably improves the doxorubicin accumulation inside the cancer cells over-express folate receptors. The rGO/Fe3O4/CS nanocomposite showed enhanced antibiofilm and antioxidant properties compared to other materials. This study's outcomes support the use of the nanocomposite in targeted chemotherapy and the potential applications in the polymer, cosmetic, biomedical, and food industries.

Download Full-text

Sr/PTA Metal Organic Framework as A Drug Delivery System for Osteoarthritis Treatment

Scientific Reports ◽

10.1038/s41598-019-54147-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Zhen Li ◽

Ying Peng ◽

Xingyu Xia ◽

Zhe Cao ◽

Yuqing Deng ◽

...

Keyword(s):

High Performance ◽

Crystal Morphology ◽

Metal Organic Framework ◽

Drug Loading ◽

Osteoarthritis Treatment ◽

Metal Organic ◽

Oa Chondrocytes ◽

Loading Amount ◽

Organic Framework

AbstractA Sr-based metal-organic framework (MOF) is introduced as ketoprofen carrier to form a comprehensive system for treating osteoarthritis (OA), and the drug loading amount and release rate is investigated. Structural characterization of the samples showed that Sr/PTA-MOF had good crystal morphology and structure, and chemical and thermal stability. Ketoprofen was successfully loaded on the MOF carrier, which had been identified by high performance liquid chromatography (HPLC) and thermogravimetric analysis (TGA). The release experiment manifested that more than 90% of ketoprofen released from Sr/PTA-MOF after 24 h, and ketoprofen delivery was mainly governed by the Higuchi model. Furthermore, cytotoxicity experiment manifested that synthesized MOF carrier had no poisonous effect on OA chondrocytes, which provided a preliminary foundation for the realization of comprehensive treating OA.

Download Full-text