Co-Electrospinning of Microbial Polyester/Gelatin and their Interaction with Fibroblasts

2007 ◽  
Vol 342-343 ◽  
pp. 201-204 ◽  
Author(s):  
So Hee Yun ◽  
Ga Young Jun ◽  
Kwan Han Yoon ◽  
Yong Soon Park ◽  
Young Jin Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Human Fibroblasts ◽  
Average Diameter ◽  
Tissue Engineering Scaffold ◽  
Promising Method ◽  
Initial Cell ◽  
Electrospinning Method ◽  
Biodegradable Poly ◽  
Mediate Cell

Biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous matrix containing gelatin was fabricated by electrospinning method. The average diameter of electrospun PHBV/Gelatin (1:1) nanofibers was 600 nm determined by FE-SEM. ATR-FTIR and ESCA measurements were used to confirm the presence of gelatin in PHBV/Gelatin nanofibers. Human fibroblasts' behavior on PHBV/Gelatin nanofibrous matrix has been investigated. Fibroblasts were well attached on the surface of control PHBV and PHBV/Gelatin nanofibers. Initial cell attachment on PHBV/Gelatin nanofibers was higher than that of control PHBV nanofibers. Gelatin has many RGD moiety that mediate cell attachment. From this reason, initial cell attachment increased on the surface of PHBV/Gelatin nanofibers. From the results, coelectrospinning of PHBV and gelatin is a promising method for tissue engineering scaffold.

The Use of Rapid Prototyping to Fabricate Liver Tissue Engineering Scaffold

2011 ◽  
Vol 328-330 ◽  
pp. 658-661
Author(s):  
Singare Sekou ◽  
Shou Yan Zhong ◽  
Zhen Zhong Sun
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Rapid Prototyping ◽  
Glycolic Acid ◽  
Tissue Engineering Scaffold ◽  
Liver Tissue Engineering ◽  
Biodegradable Poly ◽  
Future Direction ◽  
Silicone Mould ◽  
Printing Machine

In this papers, the authors described a rapid prototyping method to produce vascularized tissue such liver scaffold for tissue engineering applications. A scaffold with interconnected channel was designed using CAD environment. The data were transferred to a Polyjet 3D Printing machine (Eden 250, Object, Israel) to generate the models. Based on the 3D Printing model, a PDMS (polydimethyl-silicone) mould was created which can be used to cast the biodegradable poly (L-lactic-co-glycolic acid) (PLGA )material. The advantages and limitations of Rapid Prototyping (RP) techniques as well as the future direction of RP development in tissue engineering scaffold fabrication were reviewed.

scholarly journals Fabrication of Nanohydroxyapatite/Poly(caprolactone) Composite Microfibers Using Electrospinning Technique for Tissue Engineering Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Izzat Hassan ◽  
Tao Sun ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Average Diameter ◽  
Tissue Engineering Scaffold ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Fibrous Scaffolds ◽  
Nanosized Hydroxyapatite ◽  
Poly Caprolactone

Tissue engineering fibrous scaffolds serve as three-dimensional (3D) environmental framework by mimicking the extracellular matrix (ECM) for cells to grow. Biodegradable polycaprolactone (PCL) microfibers were fabricated to mimic the ECM as a scaffold with 7.5% (w/v) and 12.5% (w/v) concentrations. Lower PCL concentration of 7.5% (w/v) resulted in microfibers with bead defects. The average diameter of fibers increased at higher voltage and the distance of tip to collector. Further investigation was performed by the incorporation of nanosized hydroxyapatite (nHA) into microfibers. The incorporation of 10% (w/w) nHA with 7.5% (w/v) PCL solution produced submicron sized beadless fibers. The microfibrous scaffolds were evaluated using various techniques. Biodegradable PCL and nHA/PCL could be promising for tissue engineering scaffold application.

scholarly journals Fibrin Gel as an Injectable Biodegradable Scaffold and Cell Carrier for Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Yuting Li ◽  
Hao Meng ◽  
Yuan Liu ◽  
Bruce P. Lee
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Polymer Network ◽  
Tissue Engineering Scaffold ◽  
Fibrin Gel ◽  
Tissue Engineering Scaffolds ◽  
Injectable Scaffolds ◽  
Synthetic Materials ◽  
Cell Carrier ◽  
A Cell

Due to the increasing needs for organ transplantation and a universal shortage of donated tissues, tissue engineering emerges as a useful approach to engineer functional tissues. Although different synthetic materials have been used to fabricate tissue engineering scaffolds, they have many limitations such as the biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate. Fibrin gel, a biopolymeric material, provides numerous advantages over synthetic materials in functioning as a tissue engineering scaffold and a cell carrier. Fibrin gel exhibits excellent biocompatibility, promotes cell attachment, and can degrade in a controllable manner. Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network. The ability for fibrin to curein situhas been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues. Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration. Here, we review the recent advancement in developing fibrin-based biomaterials for the development of injectable tissue engineering scaffold and cell carriers.

Engineered Celery-Structured Electrospun Fibers Surface and Its Initial Cell Attachment Ability Effect

2020 ◽  
Vol 20 (7) ◽  
pp. 4336-4339
Author(s):  
Se Rim Jang ◽  
Chan Hee Park ◽  
Cheol Sang Kim
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Polymer Concentration ◽  
Initial Cell ◽  
Adhesion Ability ◽  
Topological Features ◽  
Nanofibrous Scaffolds ◽  
Initial Adhesion ◽  
A Cell ◽  
Attachment Ability

The fabrication of various types of scaffolds using electrospinning has been greatly researched for tissue engineering applications in recent times. The rapid initial cell adhesion in electrospun scaffolds helps in the rapid recovery of graft sites. The characteristics of nanofibrous scaffolds can be improved by modifying the topological features and surface of the nanofibers. Previous studies have shown that the scaffold structure is related to a cell attachment ability. In this study, we modified the surface of the fibers to mimic celery structure. It was confirmed that solvent evaporation and polymer concentration influenced the formation of the surface. This structural property can improve the initial adhesion ability of cells. Cellulose acetate solutions were prepared and tested in various concentrations (15 wt%, 20 wt%, and 30 wt%). Scanning electron microscopy (SEM), tensile test and cell experiments were performed to evaluate the physical properties and biocompatibility. The structure of the present nanofiber can be applied as a very effective scaffold and it is expected to have a positive effect in the tissue engineering field.

Fabrication of biodegradable poly(trimethylene carbonate) networks for potential tissue engineering scaffold applications

2009 ◽  
Vol 20 (9) ◽  
pp. 742-747 ◽  
Author(s):  
C. Zhang ◽  
H. Subramanian ◽  
J. J. Grailer ◽  
A. Tiwari ◽  
S. Pilla ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Engineering Scaffold ◽  
Trimethylene Carbonate ◽  
Biodegradable Poly

Characterization of PCL/HA Composite Scaffolds for Bone Tissue Engineering

2007 ◽  
Vol 342-343 ◽  
pp. 109-112 ◽  
Author(s):  
Yong Taek Hyun ◽  
Seung Eon Kim ◽  
S.J. Heo ◽  
Jung Woog Shin
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Cell Number ◽  
Bone Cell ◽  
Composite Scaffolds ◽  
Initial Cell ◽  
Salt Leaching

Porous and bioactive composite scaffolds based on poly ε-caprolactone(PCL) and hydroxyapatite(HA) were successfully fabricated by solvent casting and salt leaching method. The scaffolds have interconnected pore structure with pore size ranging from 10μm to 500μm. The pore size of PCL scaffold and PCL/HA scaffold were similar to that of the salt particles. The pore walls became thick and the small pores on the surface of macropores were formed as the HA increased. MTT assay showed that HA content did not affect initial cell attachment in both PCL scaffolds and PCL/HA scaffolds. The osteoblasts proliferated in both scaffolds, but the cell number was higher in the PCL/HA composite scaffolds. It was found that the incorporation of hydroxyapatite enhances bone cell proliferation rather than initial cell attachment in PCL/HA composite scaffolds. The results suggest that the PCL/HA composite scaffolds have a potential for the bone tissue engineering applications.

3D-Printable Biodegradable Polyester Tissue Scaffolds for Cell Adhesion

2015 ◽  
Vol 68 (9) ◽  
pp. 1409 ◽  
Author(s):  
Justin M. Sirrine ◽  
Allison M. Pekkanen ◽  
Ashley M. Nelson ◽  
Nicholas A. Chartrain ◽  
Christopher B. Williams ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Glycol Adipate ◽  
Tissue Engineering Scaffolds ◽  
Ethylene Glycol Adipate ◽  
Biodegradable Poly ◽  
Soft Tissue Engineering ◽  
Tissue Scaffolding

Additive manufacturing, or three-dimensional (3D) printing, has emerged as a viable technique for the production of vascularized tissue engineering scaffolds. In this report, a biocompatible and biodegradable poly(tri(ethylene glycol) adipate) dimethacrylate was synthesized and characterized for suitability in soft-tissue scaffolding applications. The polyester dimethacrylate exhibited highly efficient photocuring, hydrolyzability, and 3D printability in a custom microstereolithography system. The photocured polyester film demonstrated significantly improved cell attachment and viability as compared with controls. These results indicate promise of novel, printable polyesters for 3D patterned, vascularized soft-tissue engineering scaffolds.

Radiation Effect on Poly(ε-Caprolactone) Nanofibrous Scaffold

2007 ◽  
Vol 119 ◽  
pp. 95-98
Author(s):  
Youn Mook Lim ◽  
Joon Pyo Jeun ◽  
Chan Hee Jung ◽  
Jae Hak Choi ◽  
Phil Hyun Kang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Radiation Effect ◽  
Average Diameter ◽  
Nanofibrous Scaffolds ◽  
Biomimetic Scaffolds ◽  
Biodegradable Poly ◽  
Γ Ray

Nano- to micro-structured biodegradable poly(ε-caprolactone) nanofibrous scaffolds (PCL NFSs) were prepared by an electrospinning. Electrospinning has recently emerged as a leading technique for generating the biomimetic scaffolds for tissue engineering applications. The average diameter of the electrospun PCL NFSs ranged from 0.5 to 2 ㎛ depending on the solvent/nonsolvent mixture. PCL NFSs were irradiated using γ-ray and their mechanical properties and biodegradability were measured. In vitro/vivo degradation studies of the scaffolds as a function of the radiation dose were performed. The scaffolds were degraded more slowly in vitro than in vivo.

scholarly journals Functionally distinct laminin receptors mediate cell adhesion and spreading: the requirement for surface galactosyltransferase in cell spreading.

1988 ◽  
Vol 107 (5) ◽  
pp. 1863-1871 ◽  
Author(s):  
R B Runyan ◽  
J Versalovic ◽  
B D Shur
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Molecular Mechanisms ◽  
Cell Attachment ◽  
Cell Spreading ◽  
Biological Properties ◽  
Initial Cell ◽  
Laminin Receptors ◽  
Mediate Cell ◽  
A Chain

The molecular mechanisms underlying cell attachment and subsequent cell spreading on laminin are shown to be distinct form one another. Cell spreading is dependent upon the binding of cell surface galactosyltransferase (GalTase) to laminin oligosaccharides, while initial cell attachment to laminin occurs independent of GalTase activity. Anti-GalTase IgG, as well as the GalTase modifier protein, alpha-lactalbumin, both block GalTase activity and inhibited B16-F10 melanoma cell spreading on laminin, but not initial attachment. On the other hand, the addition of UDP galactose, which increases the catalytic turnover of GalTase, slightly increased cell spreading. None of these reagents had any effect on cell spreading on fibronectin. When GalTase substrates within laminin were either blocked by affinity-purified GalTase or eliminated by prior galactosylation, cell attachment appeared normal, but subsequent cell spreading was totally inhibited. The laminin substrate for GalTase was identified as N-linked oligosaccharides primarily on the A chain, and to a lesser extent on B chains. That N-linked oligosaccharides are necessary for cell spreading was shown by the inability of cells to spread on laminin surfaces pretreated with N-glycanase, even though cell attachment was normal. Cell surface GalTase was distinguished from other reported laminin binding proteins, most notably the 68-kD receptor, since they were differentially eluted from laminin affinity columns. These data show that surface GalTase does not participate during initial cell adhesion to laminin, but mediates subsequent cell spreading by binding to its appropriate N-linked oligosaccharide substrate. These results also emphasize that some of laminin's biological properties can be attributed to its oligosaccharide residues.

Development of Rapid Cell Recovery System Using Temperature-Responsive Nanofiber Surfaces

2007 ◽  
Vol 342-343 ◽  
pp. 249-252 ◽  
Author(s):  
Young Gwang Ko ◽  
Hyeon Ae Jeon ◽  
Kwan Han Yoon ◽  
Young Chul Kim ◽  
Chang Hyun Ahn ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Sessile Drop ◽  
Cell Attachment ◽  
Single Cells ◽  
Human Fibroblasts ◽  
Film Surface ◽  
Contact Angles ◽  
Cell Recovery ◽  
Water Contact ◽  
Initial Cell

PHBV ultrafine fibers were fabricated by electrospinning process. Electrospun PHBV fiber structures revealed randomly aligned fibers with average diameter of 400 nm. PIPAAm was grafted on the surface of PHBV nanofibrous mat by electron beam irradiation. PIPAAm-grafted PHBV mats were determined by ATR-FTIR and ESCA. Water contact angles were determined by a sessile drop method at 20 and 37. To examine the tissue compatibility, human fibroblasts were evenly seeded onto PIPAAm-grafted PHBV mat and cast film, ungrafted PHBV mat and film. Attached and spread fibroblasts on nanofibrous mat were proliferated more rapidly than that of flat film surface. Initial cell attachment on PIPAAm-grafted surfaces was higher than ungrafted surfaces. The surface property changed to hydrophilic by PIPAAm graft, which increased initial cell attachment. Detachment of single cells from PIPAAm-grafted PHBV matrixes was measured by low temperature treatment after incubation at 37. Cultured cells were rapidly detached from PIPAAm-grafted PHBV mat compared with film. With porous mats, the water molecules easily reach to grafted PIPAAm from underneath and peripheral to the attached cells, resulting in rapid hydration of grafted PIPAAm molecules and detachment of the cells.

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