SUSCEPTIBILITY OF THE MIGRATORY GRASSHOPPER, MELANOPLUS SANGUINIPES (FAB.) (ORTHOPTERA: ACRIDIDAE), TO BEAUVERIA BASSIANA (BALS.) VUILLEMIN (HYPHOMYCETE): INFLUENCE OF RELATIVE HUMIDITY

1987 ◽  
Vol 119 (10) ◽  
pp. 901-907 ◽  
Author(s):  
S. Marcandier ◽  
G.G. Khachatourians
Keyword(s):  
Relative Humidity ◽  
Beauveria Bassiana ◽  
Mycelial Growth ◽  
Field Testing ◽  
Natural Mortality ◽  
Melanoplus Sanguinipes ◽  
Body Coloration ◽  
Daily Rate ◽  
Significant Difference ◽  
Semi Arid

AbstractIn laboratory tests, Melanoplus sanguinipes (Fab.) was susceptible to infection by Beauveria bassiana (Bals.) Vuillemin conidia. Infection occurred independently of the relative humidity (RH) (12, 33, 76, 100% RH). No significant difference was observed in the final percentage mortality of the treated grasshoppers and in the lethal times (LT50)(P > 0.05) under the RH conditions studied. However, the daily rate of mortality after treatment was higher at 76% RH than at 33% RH. High natural mortality occurred at 100% RH.At 100% RH, treatment with B. bassiana was associated with an outgrowth of microorganism that created septicemic conditions in the absence of the characteristic symptoms of mycosis. Below 100% RH, mycelial growth on cadavers was never observed, even though partial or total insect mummification occurred, and a pink body coloration on approximately 80% of the insects indicated Beauveria as the killing agent. Once transferred to 100% RH, no more than 5% of the treated grasshoppers exhibited external mycelial growth.The microenvironment at the cuticular level of the grasshopper allows expression of conidial pathogenicity regardless of ambient RH. Hence, together, these observations suggest the feasibility of field testing with B. bassiana as a bioinsecticide against M. sanguinipes in semi-arid climatic areas.

scholarly journals Comparison of Fenugreek Crop Evapotranspiration Measured by a Micro-lysimeter, Field Water Balance Method and Automatic Closed Canopy Chamber

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Deepak Kumar
Keyword(s):  
Relative Humidity ◽  
Field Testing ◽  
Sensor Data ◽  
Crop Evapotranspiration ◽  
Short Period ◽  
Significant Difference ◽  
Measurement Mode ◽  
Closed Canopy ◽  
Data Pair ◽  
Canopy Chamber

The attempt was to design and develop an automatic closed canopy chamber (ACCC) having dimension of 1.2 m×1.2 m× 1.2 m for crop evapotranspiration measurement by using transparent acrylic sheet of 4 mm thickness. Between two small fans a temperature and relative humidity sensor was used to measure vapor density. The intermediate circuit was developed for making automation system in ACCC. The arduino based coding was developed as per desired logic operation. The top lead of chamber was automatically closed for 2 minutes when inside and outside temperature and relative humidity of ambient air were similar. During measurement mode of ACCC, the two fans were started automatically. After measurement mode, fans were automatically stopped and top lead was opened. The ACCC was calibrated by evaporating mass of water from water filled tray which was placed inside the automatic closed canopy chamber. The validation of the developed ACCC were made using micro-lysimeters (MLS) having size of 0.2 m × 0.2 m × 0.2 m by growing shallow rooted crop like fenugreek. The depth of irrigation was computed based on soil moisture content before irrigation and field capacity. The field testing of ACCC was made by placing chamber in plots of fenugreek crop. The irrigation was applied by drip irrigation as per crop water consumption. The sensor sensed and recorded the instantaneous temperature and relative humidity at 1 second interval for 24 hours. Two sample t-tests were done to compare the data pair of crop evapotranspiration obtained by the MLS inside the ACCC with that of outside the ACCC to ascertain whether there is any effect of the change in micro-climate for a short period of 2 minute on the crop growth physiological processes. Also, the data pair of crop evapotranspiration measured by the MLs, ACCC using the sensor data of temperature-relative humidity were compared and statistically analyzed through t-test. Similarly, the data pair of ETC measured by the FWB (Field Water Balance Method) and ACCC using the sensor data of temperature-relative humidity were also compared and statistically analyzed through t-test. The calibration factor of the ACCC was found as 1.666. The results revealed that there was no significant difference in the crop evapotranspiration measured by the MLs inside and outside ACCC. Also in case of validation and field testing of ACCC, there were no significant difference between the ETC measured by the ACCC, MLs and FWB at 95 percent confidence level. This implies that there are no effects of the change in micro-climate for a short period of 2 minutes in the chamber, on the plant physiological processes. The ETc rate of fenugreek increases as sun rises and reaches the peak after one to two hour from mid-day and then continuously decreases with time. During validation and field testing of ACCC, the fenugreek crop coefficients varied from 0.72 to 1.04 and 0.69 to 1.02 respectively. The developed ACCC is portable as well as more comfortable and cost effective compared to the lysimeter for the measurement of the actual crop evapotranspiration and the crop coefficient.

scholarly journals Antifungal Activity of Propyl Disulfide from Neem (Azadirachta indica) in Vapor and Agar Diffusion Assays against Anthracnose Pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango Fruit

2021 ◽  
Vol 9 (4) ◽  
pp. 839
Author(s):  
Muhammad Rafiullah Khan ◽  
Vanee Chonhenchob ◽  
Chongxing Huang ◽  
Panitee Suwanamornlert
Keyword(s):  
Antifungal Activity ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Acutatum ◽  
Model Parameters ◽  
Pathogenicity Test ◽  
Agar Diffusion ◽  
Wide Range ◽  
Significant Difference ◽  
Diffusion Assay

Microorganisms causing anthracnose diseases have a medium to a high level of resistance to the existing fungicides. This study aimed to investigate neem plant extract (propyl disulfide, PD) as an alternative to the current fungicides against mango’s anthracnose. Microorganisms were isolated from decayed mango and identified as Colletotrichum gloeosporioides and Colletotrichum acutatum. Next, a pathogenicity test was conducted and after fulfilling Koch’s postulates, fungi were reisolated from these symptomatic fruits and we thus obtained pure cultures. Then, different concentrations of PD were used against these fungi in vapor and agar diffusion assays. Ethanol and distilled water were served as control treatments. PD significantly (p ≤ 0.05) inhibited more of the mycelial growth of these fungi than both controls. The antifungal activity of PD increased with increasing concentrations. The vapor diffusion assay was more effective in inhibiting the mycelial growth of these fungi than the agar diffusion assay. A good fit (R2, 0.950) of the experimental data in the Gompertz growth model and a significant difference in the model parameters, i.e., lag phase (λ), stationary phase (A) and mycelial growth rate, further showed the antifungal efficacy of PD. Therefore, PD could be the best antimicrobial compound against a wide range of microorganisms.

Growth performance of Acheta domesticus and Gryllus bimaculatus production reared under improvised cage system for increased returns and food security

2020 ◽  
pp. 1-10
Author(s):  
C.A. Ngonga ◽  
C.O. Gor ◽  
E.A. Okuto ◽  
M.A. Ayieko
Keyword(s):  
Relative Humidity ◽  
Growth Performance ◽  
Additive Model ◽  
Acheta Domesticus ◽  
Cotton Wool ◽  
Conventional System ◽  
Gryllus Bimaculatus ◽  
Generalised Additive Model ◽  
Cage System ◽  
Significant Difference

Cricket farming is emerging as a new venture in Kenya poised to help alleviate protein deficiency and improve household living standards. However, competing, limited and unaffordable rearing systems constrain productivity and optimisation of this new enterprise. This study sought to evaluate the growth performance of Acheta domesticus and Gryllus bimaculatus reared in improvised cage system to assess its technical effectiveness. Twenty-day old of both species of crickets were separately reared in improvised and conventional cage systems for comparison purposes. Whereas an improvised cage system is a set of structure devised using locally available materials especially where the standard materials are limited, a conventional cage system is a set of structure made of standard and ordinary materials. The improvised system comprised of bamboo hideouts, clean scrap blankets for drinking and laying, cut bamboo stem as drinking platter and the plywood-based cages while conventional system comprised of egg carton hideouts, cotton-wool for drinking and laying, plastic petri-dishes, and plastic buckets. Each group of crickets (100 live crickets) was daily provided with equal amount of feed and water. Average weekly temperature and relative humidity profiles were recorded using HOBO data loggers. Further, 49 live crickets per treatment were randomly sampled and weighed weekly. Generalised additive model and analysis of variance were adopted to model the data therefrom using R. The cage system had a significant effect on the growth performance of the crickets. The mean weight of the cricket species differed, but not the interaction with the cage system. Similarly, species, temperature and relative humidity also influenced the growth performance. Compared to the conventional system, improvised cage system showed better results in realising high cricket productivity though there was no significant difference in growth performance between the two systems. The focus therefore should be to evaluate the egg productivity in scrap blanket in relation to cotton wool and cost effectiveness in improvised system to inform future farm decisions.

scholarly journals Productive performance and parasitological control of kids supplemented with umbu fruits (Spondias tuberosa Arruda)

2016 ◽  
Vol 17 (3) ◽  
pp. 520-528
Author(s):  
Thiago Vinicius Costa NASCIMENTO ◽  
Daniel Maia NOGUEIRA ◽  
Nilton de Brito CAVALCANTE
Keyword(s):  
Weight Gain ◽  
Arid Zone ◽  
Experimental Period ◽  
Gastrointestinal Nematodes ◽  
Daily Weight Gain ◽  
Productive Performance ◽  
Buffel Grass ◽  
Significant Difference ◽  
Semi Arid Region ◽  
Semi Arid

Summary Native fruits from Caatinga vegetation can be an important alternative to improve the productive performance of kids in the semi-arid region of Brazil. This work aimed to evaluate the effect of supplementation with Umbuzeiro fruits in natura over the weight gain and anthelmintic control of kids kept in Buffel grass pasture in the semi-arid zone of Pernambuco, Brazil. Twenty-four castrated, crossbreed kids were allocated into three treatments: 1) Control (n = 8) fed exclusively with Buffel grass; 2) Umbu 1x (n = 8) fed with fruits once a week and 3) Umbu 3x (n = 8) fed with fruits for three times a week. The following parameters were evaluated: variation of body weight, faecal egg counts (FEC) and coproculture. Overall, the average consumption of Umbu fruits in natura was 1.48 kg/animal/day, which corresponded to 133.5 g daily dry matter intake (DMI/animal/day). There were no significant difference (P>0.05) for any parameters evaluated. During the experimental period, the overall daily weight gain was variable between 108.75 to 116.70 g/animal/day and the average FEC was 436 eggs. In the present study, the goat kids supplemented with fresh umbu fruits showed a good productive performance, however the supplementation with umbu did not control the infestation of gastrointestinal nematodes in kids.

scholarly journals Susceptibility of Gyropsylla spegazziniana (Lizer & Trelles) (Hemiptera: Psyllidae) to Beauveria bassiana (Bals.) Vuill.

2013 ◽  
Vol 80 (3) ◽  
pp. 363-366 ◽  
Author(s):  
Luis Francisco Angeli Alves ◽  
Marina Andressa Formentini ◽  
André Luis Pereira Fanti ◽  
Maria Elena Schapovaloff ◽  
Ionete Lúcia Milani Barzotto
Keyword(s):  
Relative Humidity ◽  
Beauveria Bassiana ◽  
Natural Enemies ◽  
Total Mortality ◽  
Yerba Mate ◽  
Laboratory Setting ◽  
Pest Insects ◽  
Insect Mortality ◽  
Zoophthora Radicans ◽  
High Level

Gyropsylla spegazziniana is one of the most prominent pest insects of yerba mate culture in all production regions in Brazil, Argentina and Paraguay. Natural enemies have been recorded on G. spegazziniana nymphs and adults, including the Zoophthora radicans entomopathogenic fungus. Since there are no reports of Beauveria bassiana, the aim of this study was to register its pathogenicity with respect to this insect in a laboratory setting. Yerba mate branches were kept in glass flasks with water and we infested each leaf with 20 newly-emerged nymphs. We prepared three replicates per treatment. We sprayed conidia suspensions (1 × 109 conidia/mL) onto the branches, which we transferred to cages and kept in an acclimatized room (26 ± 1°C, 70 ± 10% of relative humidity (R.H.) with a photophase of 14 hours). We evaluated insect mortality daily, and after five days we observed a high level of pathogenicity due to the presence of the fungus, that could be visually observed on the cadaver. The total mortality varied from 25 to 70% (respectively for Unioeste 4 and CG 716) and the confirmed mortality was 30% for Unioeste 52, revealing the fungus' potential. However, more studies are necessary in order to evaluate the strains of this fungus, as well as other species.

scholarly journals Detection of Hydroxychloroquine Retinal Toxicity by Automated Perimetry in 60 Rheumatoid Arthritis Patients with Normal Fundoscopic Findings

2015 ◽  
Vol 8 (3) ◽  
pp. 59 ◽  
Author(s):  
Qader Motarjemizadeh ◽  
Naser Samadi Aidenloo ◽  
Mohammad Abbaszadeh
Keyword(s):  
Rheumatoid Arthritis ◽  
Early Detection ◽  
Anterior Segment ◽  
Field Testing ◽  
Retinal Toxicity ◽  
Screening Tests ◽  
Automated Perimetry ◽  
Cross Sectional ◽  
Dry Eyes ◽  
Significant Difference

<p>Hydroxychloroquine (HCQ) is an antimalarial drug used extensively in treatment of autoimmune diseases such as rheumatoid arthritis. Retinal toxicity is the most important side effects of this drug. Even after the drug is discontinued, retinal degeneration from HCQ can continue to progress. Consequently, multiple ophthalmic screening tests have been developed to detect early retinopathy. The aim of the current study was to evaluate the value of central 2-10 perimetry method in early detection of retinal toxicity. This prospective cross-sectional investigation was carried out on 60 rheumatoid arthritis patients, who had been receiving HCQ for at least 6 months and still were on their medication (HCQ intake) at the time of enrollment. An ophthalmologist examined participants using direct and indirect ophthalmoscopy. Visual field testing with automated perimetry technique (central 2-10 perimetry with red target) was performed on all included subjects twice in 6 months interval: The first one at the time of enrollment and the second one 6 months later. Males and females did not show any significant difference in terms of age, duration of therapy, daily and cumulative HCQ dose, anterior or posterior segment abnormalities, hypertension, body mass index, and best corrected visual acuity. Anterior segment was abnormal in 9 individuals including 3 subjects with macular pigmentary changes, 4 individuals with cataract and 2 cases with dry eyes. Moreover, 12 subjects had retinal pigmented epithelium (RPE) in their posterior segments. After 6 months, depressive changes appeared in 12 subjects. Additionally, HCQ therapy worsened significantly the perimetric results of 5 (55.6%) patients with abnormal anterior segment. A same trend was observed in perimetric results of 6 (50.0%) subjects with abnormal posterior segments (P=0.009). The daily dose of HCQ (P=0.035) as well as the cumulative dose of hydroxychloroquine (P=0.021) displayed statistically significant associations with perimetric results. Central 2-10 perimetry is a useful method for early detection of HCQ retinal toxicity, but more comprehensive studies, with larger sample size, longer-term follow-up and more precise techniques are mandatory to confirm HCQ retinal toxicity.</p>

scholarly journals Bacterial Inhibition on Beauveria bassiana Contributes to Microbiota Stability in Delia antiqua

2021 ◽  
Vol 12 ◽  
Author(s):  
Fangyuan Zhou ◽  
Yunxiao Gao ◽  
Mei Liu ◽  
Letian Xu ◽  
Xiaoqing Wu ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Mycelial Growth ◽  
Bacterial Species ◽  
Management Strategies ◽  
Underlying Mechanism ◽  
Conidial Germination ◽  
Delia Antiqua ◽  
Bacterial Microbiota ◽  
Fungal Microbiota ◽  
Microbial Environment

Given the multiple roles of associated microbiota in improving animal host fitness in a microbial environment, increasing numbers of researchers have focused on how the associated microbiota keeps stable under complex environmental factors, especially some biological ones. Recent studies show that associated microbiota interacts with pathogenic microbes. However, whether and how the interaction would influence microbiota stability is limitedly investigated. Based on the interaction among Delia antiqua, its associated microbiota, and one pathogen Beauveria bassiana, the associated microbiota's response to the pathogen was determined in this study. Besides, the underlying mechanism for the response was also preliminarily investigated. Results showed that B. bassiana neither infect D. antiqua larvae nor did it colonize inside the associated microbiota, and both the bacterial and fungal microbiota kept stable during the interaction. Further experiments showed that bacterial microbiota almost completely inhibited conidial germination and mycelial growth of B. bassiana during its invasion, while fungal microbiota did not inhibit conidial germination and mycelial growth of B. bassiana. According to the above results, individual dominant bacterial species were isolated, and their inhibition on conidial germination and mycelial growth of B. bassiana was reconfirmed. Thus, these results indicated that bacterial instead of fungal microbiota blocked B. bassiana conidia and stabilized the associated microbiota of D. antiqua larvae during B. bassiana invasion. The findings deepened the understanding of the role of associated microbiota–pathogen microbe interaction in maintaining microbiota stability. They may also contribute to the development of novel biological control agents and pest management strategies.

scholarly journals Development of Maquette to Promote Primary School Students’ Critical Thinking Ability in Social Sciences

2019 ◽  
Author(s):  
Rindang Hayom Sasami ◽  
Sujarwo Sujarwo
Keyword(s):  
Critical Thinking ◽  
Field Testing ◽  
T Test ◽  
Research Subjects ◽  
Main Field ◽  
School Students ◽  
Gain Score ◽  
Critical Thinking Ability ◽  
Significant Difference ◽  
Thinking Ability

This research aimed to analyse the feasibility of the use of maquette and the effectiveness of maquette in improving students’ critical thinking ability. The research model employed was the development research model developed by Borg & Gall. The research subjects were third graders. The testing was divided into preliminary field testing, main field testing, and operational field testing. The analysis of the medium feasibility was based on the assessment by media experts, the assessment by subject matter experts, students’ response, and teacher’s response. The analysis of maquette effectiveness was carried out using a t-test (two independent samples) to figure out the difference and gain score of the experimental class for the purpose of identifying the improvement after the use of maquette. In the feasibility analysis, a score of 88 in the category “feasible” was gained from the assessment by a media expert and a score of 85 in the category “highly feasible” was gained from the assessment by a subject matter expert. From the preliminary field testing, scores of 60.5 and 65 in the category “highly feasible” were gained for the students’ response and teacher’s response, respectively. Meanwhile, from the main field testing, scores of 56.1 and 66.5 in the category “highly feasible” were gained for the students’ response and teacher’s response to the maquette use, respectively. The t-test results show a significant value in the critical thinking, namely.000 <.05, indicating a significant difference between the control class and the experimental class. The gain score was 0.5 and fell into the “moderate” category. Thus, it can be concluded that maquette is effective in improving critical thinking ability.

scholarly journals DESENVOLVIMENTO DE MUDAS DE CAFEEIRO PRODUZIDAS EM TUBETES SOB MALHAS TERMO-REFLETORAS E MALHA NEGRA

Irriga ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 52 ◽  
Author(s):  
Vinícios Maia Costa ◽  
Marcos Vinícios Folegatti ◽  
Roberto Terumi Atarassi ◽  
Sérgio Nascimento Duarte ◽  
Nildo Da Silva Dias ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Solar Radiation ◽  
Leaf Area ◽  
Coffea Arabica ◽  
Dry Matter ◽  
Significant Difference ◽  
Stalk Diameter ◽  
E Mail ◽  
Coffea Arabica L

DESENVOLVIMENTO DE MUDAS DE CAFEEIRO PRODUZIDAS EM TUBETES SOB MALHAS TERMO-REFLETORAS E MALHA NEGRA VINÍCIUS MAIA COSTA1; MARCOS VINÍCIUS FOLEGATTI2; ROBERTO TERUMI ATARASSI3; SÉRGIO NASCIMENTO DUARTE4; NILDO DA SILVA DIAS5 E CLAUDIO RICARDO DA SILVA6.  [1] Mestre em Agronomia, Depto. de Engenharia de Biossistemas, ESALQ-USP, Piracicaba – SP. E-mail: [email protected]. Titular, Depto. de Engenharia de Biossistemas, ESALQ-USP, Piracicaba – SP. E-mail: [email protected] de Ciências Agrárias, UFU, Av. Amazonas, s/n, Bloco 2E, Campus Umuarama, 38400-902, Uberlândia-MG. E-mail: [email protected]. Associado, Depto. de Engenharia de Biossistemas, ESALQ-USP, Piracicaba – SP. E-mail: [email protected] Associado 2, Departamento de Ciências Ambientais e Tecnológicas, Universidade Federal Rural do Semi-Árido, Mossoró, RN. E-mail: [email protected] de Ciências Agrárias, UFU, Av. Amazonas, s/n, Bloco 2E, Campus Umuarama, 38400-902, Uberlândia-MG. E-mail: [email protected].  1 RESUMO Este experimento avaliou, em casa de vegetação, o desenvolvimento de mudas de cafeeiro produzidas em tubetes, sob o efeito de malhas utilizadas para sombreamento. Os tratamentos foram a tradicional malha negra 50% e as malhas termo-refletoras com atenuação da radiação solar de 40, 50, 60 e 70%. O delineamento experimental adotado foi em blocos casualizados contendo os 5 tratamentos, com 4 repetições, totalizando-se assim 20 parcelas. Os resultados mostram que temperatura do ar pouco variou no microclima protegido das parcelas, assim como a umidade relativa do ar e a transmissividade global (TRG) e fotossinteticamente ativa (TRFA). Verificou-se que malhas com menor atenuação da radiação solar apresentaram em geral maior transmissividade. A área foliar não apresentou diferença significativa entre os tratamentos; já para altura da planta e diâmetro do caule, verificou-se que as mudas produzidas sob as malhas termo-refletoras 40 e 50% foram superiores àquelas produzidas sob malha negra 50% e malhas termo-refletoras 60 e 70%, respectivamente. Nas avaliações de matéria seca, verificou-se que as mudas produzidas sob as malhas termo-refletoras 40% e 50% e malha negra 50% acumularam mais matéria seca comparativamente àquelas produzidas sob malhas termo-refletoras 60 e 70%. Assim, concluiu-se que as malhas termo-refletoras 40 e 50% e negra 50% apresentaram melhores resultados que as malhas termo-refletoras 60 e 70%. Palavras-chave: Coffea arábica L., sombreamento, ambiente protegido.  COSTA, V.M.; FOLEGATTI, M.V.; ATARASSI, R.T.; DUARTE, S.N.; DIAS, N.S.; SILVA, C.R. DEVELOPMENT OF COFFEE SEEDLINGS PRODUCED IN PLASTIC TUBESUNDER TERMO-REFLECTIVE AND BLACK SCREENS   2 ABSTRACT This experiment evaluated, in greenhouse conditions, the development of coffee seedlings produced in plastic tubes, under the effect of screens used for shading. The treatments were the traditional black screen 50% and the thermo reflective screens with attenuation of the solar radiation of 40, 50, 60 and 70%. The adopted experimental delineation was randomized blocks with 5 treatments and 4 replicates, making up 20 parcels. The result showed that the temperature of air gently varied in the parcels, as well as the air relative humidity.  Relating to global transmissivity (TRG) and photosynthetically active transmissivity (TRFA), it was verified that screens with less attenuation of the solar radiation presented higher transmissivity in general. The leaf area did not present significant difference among the treatments and seedlings height and stalk diameter, the seedlings produced under thermo-reflective screens 40% and 50% were superior to those produced under black screen 50% and thermo-reflective screen 60% and 70%. In the evaluations of dry matter, the seedlings produced under thermo-reflective screens 40% and 50%, and black screen 50% comparatively accumulated more dry matter than those produced under thermo-reflective screens 60% and 70%. Thus, it can be concluded that the thermo-reflective screens 40% and 50% and black screen 50% presented better results than the thermo-reflective screens 60% and 70%. Keywords: Coffea arábica L., shading, greenhouse.

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