MMP-7 and MMP-9 are overexpressed in the synovial tissue from severe temporomandibular joint dysfunction

Matrix metalloproteinases (MMPs) are tissue-enzymes that play a key role during the remodeling process, such as in inflammatory diseases. MMP-7 and MMP-9 have been shown to be implicated in extracellular matrix homeostasis and in joint disc remodeling. The objective of this study was to determine the relation of MMP-7 and MMP-9 expression with severe temporomandibular joint dysfunction, in particular with anterior disk displacement without reduction (ADDwoR), using an immunohistochemical approach. Therefore, twenty human temporomandibular synovia in the test group and ten in the control group were collected. The results showed there was a statistically significant difference (P<0.001) for morphometric and densitometric analysis of both detected MMPs in control and test groups. In conclusion, MMP-7 and MMP-9 were overexpressed in the synovial tissue of patients with ADDwoR.

Download Full-text

Peri-implantitis and extracellular matrix antibodies: A case–control study

European Journal of Dentistry ◽

10.4103/ejd.ejd_28_17 ◽

2017 ◽

Vol 11 (03) ◽

pp. 340-344 ◽

Cited By ~ 5

Author(s):

Piero Papi ◽

Stefano Di Carlo ◽

Daniele Rosella ◽

Francesca De Angelis ◽

Mario Capogreco ◽

...

Keyword(s):

Extracellular Matrix ◽

Case Control Study ◽

Test Group ◽

Case Control ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Fisher Exact Test ◽

Type I ◽

Significant Difference ◽

Control Study

ABSTRACT Objective: The aim of this case–control study was to compare patients with a healthy peri-implant environment and patients affected by peri-implantitis, evaluating the occurrence of antibodies to extracellular matrix (ECM) molecules. The authors hypothesized the presence of ECM autoantibodies in serum of peri-implantitis patients. Materials and Methods: Patients were divided into two groups: one with dental implants with a diagnosis of peri-implantitis and one control group with implants classified as being “healthy.” Enzyme-linked immunosorbent assay was performed on patients' sera to detect human antibodies to type I, III, IV, and V collagens, laminin, and fibronectin. Fisher exact test was performed to evaluate statistical association, with a significant P < 0.05. Results: Forty-two patients were enrolled in this study, 27 females (64.28%) and 15 males (35.72%) with a mean age of 53 ± 29.69 years (age range 32–74). The presence of antibodies to CIII was recorded in 6/21 (28.57%) patients of test group, compared to just 2/21 (9.52%) for the control group, showing a statistically significant difference (P < 0.05). Other antibodies tested were found to be not statistically significant or absent. Conclusions: Within the limitations of this study, it can be concluded that further studies, with larger sample and different design, are necessary to address the research purpose, evaluating possible associations between anti-ECM antibodies and peri-implantitis.

Download Full-text

EVALUATION OF CEPHALOMETRIC ANALYSIS IN PATIENTS WITH BILATERAL CONDYLAR RETROPOSITION IN TEMPOROMANDIBULAR DYSFUNCTION

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії ◽

10.31718/2077-1096.20.3.41 ◽

2020 ◽

Vol 20 (3) ◽

pp. 41-46

Author(s):

V.V. Vovk ◽

V.P. Nespriadko

Keyword(s):

Temporomandibular Joint ◽

Test Group ◽

Masticatory Muscles ◽

Control Group ◽

Cephalometric Analysis ◽

Temporomandibular Joint Dysfunction ◽

Mandibular Growth ◽

Facial Skull ◽

Condylar Position ◽

Canine Guidance

Relevance. During the dentoalveolar growth and development, the direction and growth type of facial skull play a very important role. Development of esthetical symmetry, functional relationship, and clinical disharmony depends on skull parameters. Temporomandibular joint dysfunction is one of the most common diseases nowadays. That is why early identification of risk factors is among the priorities. This study aimed at evaluating occlusional and skeletal specific characteristics in patients with temporomandibular joint dysfunction, comparing different cephalometric findings and CT findings relating to condylar position. Materials and methods. The study included 45 individuals. The study group consisted of 26 patients with bilateral condylar retroposition. Control group was made up of 19 patients. The participants underwent clinical examination, functional testing, palpation of masticatory muscles, occlusion diagnosis by applying Baush articulating film 200,100,8 microns, cephalometric analysis: Tweed, Kim, Jarabak, Ricketts, computed tomography of temporomandibular joint. Statistical analysis was carried out by the program IBM SPSS Statistic Base v.22. Results. There were no pathological contacts during evaluation of static and dynamic occlusion, laterotrusion as «canine guidance». The patients of the test group had bilateral posterior joint fissure reduced right-median 1,96, left-median 1,81. In control group the position was right-average 2,75 and left-median 2,67 The patients in the control and test groups the indices Tweed <FMA average were 19,6°/ median 21,7°; <IMPA average 97,9°/ median 97,8°; Kim ODI median was 68,9°/73,74°; Ricketts Overbite median 2.1 mm /1,9 mm, Overjet median was 2,9 mm /2,85 mm, PM-Xi-ANS median was 44,1°/43,75°, <NPog-FH median was 91°/86,85°,<NBa-PtGn median was 91,1°/90,1°, <MeGo-FH median was 18,7°/22,4°; Jarabak <N-S-Ar average was 127,2°/125,6 °. Conclusions. In the patients with bilateral condylar retropositionm the joint fissure is reduced. Cephalometric analysis demonstrated the following: Tweed <FMA, <IMPA; Kim ODI; Ricketts Overbite, Overjet, PM-Xi-ANS, <MeGo-FH; Jarabak <N-S-Ar can not be used as diagnostic criteria of bilateral condylar retroposition. Ricketts <NPog-FH in the test group showed the mandibular retroposition, but with normal type of mandibular growth <NBa-PtGn. It can be used as one of the primary cephalometric diagnostic indicator of bilateral condylar retroposition.

Download Full-text

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

Journal of Clinical and Health Sciences ◽

10.24191/jchs.v1i1.5850 ◽

2016 ◽

Vol 1 (1) ◽

pp. 22

Author(s):

Nazli Zainuddin ◽

Nurul Azira Mohd Shah ◽

Rosdan Salim

Keyword(s):

Allergic Rhinitis ◽

Side Effects ◽

Coconut Oil ◽

Test Group ◽

Nasal Symptom ◽

Control Group ◽

Virgin Coconut Oil ◽

Control Groups ◽

Significant Difference ◽

And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

Download Full-text

Clinical and Immunological Efficacy of Mangosteen and Propolis Extracted Complex in Patients with Gingivitis: A Multi-Centered Randomized Controlled Clinical Trial

Nutrients ◽

10.3390/nu13082604 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2604

Author(s):

Jin-Young Park ◽

Kyung-A Ko ◽

Ji-Yeong Lee ◽

Jae-Woon Oh ◽

Hyun-Chang Lim ◽

...

Keyword(s):

Patient Reported Outcomes ◽

Daily Intake ◽

Test Group ◽

Control Group ◽

Controlled Clinical Trial ◽

Control Groups ◽

Immunological Parameters ◽

Significant Difference ◽

Patient Reported ◽

And Control

Background: Mangosteen and propolis extracts (MAEC) have been potential therapeutic agents known to exhibit powerful antioxidant and anti-inflammatory properties. The aim of the current study was to evaluate the clinical and immunological efficacy of MAEC as well as safety and patient-reported outcomes (PROMs) on gingivitis and incipient periodontitis. Methods: This study was performed on 104 patients diagnosed with gingivitis or incipient periodontitis. At baseline, the participants were randomly allocated to either the test group, with daily intake of a single capsule containing 194 mg of MAEC for eight weeks, or control group, with placebo. Clinical periodontal evaluation and immunological parameters from saliva and gingival sulcular fluid were assessed at baseline, four, and eight weeks. Individual PROMs were assessed by OHIP-14 questionnaires. Results: There was a significant difference of modified gingival index at four and eight weeks between the test and control groups. In the test group, crevicular interleukin (IL)-6 was reduced, and the salivary matrix metalloproteinase (MMP)-9 was increased after eight weeks. PROMs were improved up to four weeks compared to placebo. Conclusion: Oral administration of MAEC would have a potential to reduce gingival inflammation clinically and immunologically in the patients with gingivitis and incipient periodontitis.

Download Full-text

Assessment of the Tissue Response to Modification of the Surface of Dental Implants with Carboxyethylphosphonic Acid and Basic Fibroblastic Growth Factor Immobilization (Fgf-2): An Experimental Study on Minipigs

Biology ◽

10.3390/biology10050358 ◽

2021 ◽

Vol 10 (5) ◽

pp. 358

Author(s):

Javier Aragoneses ◽

Ana Suárez ◽

Nansi López-Valverde ◽

Francisco Martínez-Martínez ◽

Juan Manuel Aragoneses

Keyword(s):

Surface Treatment ◽

Test Group ◽

Mean Value ◽

Control Group ◽

P Value ◽

Implant Surface ◽

Bone Contact ◽

Bone Implant ◽

Significant Difference ◽

The Mean

The aim of this study was to evaluate the effect of implant surface treatment with carboxyethylphosphonic acid and fibroblast growth factor 2 on the bone–implant interface during the osseointegration period in vivo using an animal model. The present research was carried out in six minipigs, in whose left tibia implants were inserted as follows: eight implants with a standard surface treatment, for the control group, and eight implants with a surface treatment of carboxyethylphosphonic acid and immobilization of FGF-2, for the test group. At 4 weeks after the insertion of the implants, the animals were sacrificed for the histomorphometric analysis of the samples. The means of the results for the implant–bone contact variable (BIC) were 46.39 ± 17.49% for the test group and 34.00 ± 9.92% for the control group; the difference was not statistically significant. For the corrected implant–bone contact variable (BICc), the mean value of the test group was 60.48 ± 18.11%, and that for the control group, 43.08 ± 10.77%; the difference was statistically significant (p-value = 0.035). The new bone formation (BV/TV) showed average results of 27.28 ± 3.88% for the test group and 26.63 ± 7.90% for the control group, meaning that the differences were not statistically significant (p-value = 0.839). Regarding the bone density at the interthread level (BAI/TA), the mean value of the test group was 32.27 ± 6.70%, and that of the control group was 32.91 ± 7.76%, with a p-value of 0.863, while for the peri-implant density (BAP/TA), the mean value of the test group was 44.96 ± 7.55%, and that for the control group was 44.80 ± 8.68%, without a significant difference between the groups. The current research only found a significant difference for the bone–implant contact at the cortical level; therefore, it could be considered that FGF-2 acts on the mineralization of bone tissue. The application of carboxyethylphosphonic acid on the surface of implants can be considered a promising alternative as a biomimetic coating for the immobilization of FGF-2. Despite no differences in the new bone formation around the implants or in the interthread or peri-implant bone density being detected, the biofunctionalization of the implant surface with FGF-2 accelerates the mineralization of the bone–implant interface at the cortical level, thereby reducing the osseointegration period.

Download Full-text

Resorbable Collagen Barrier Impeding the Extrusion of Obturating Material in Primary Molars Undergoing Resorption – A Randomized Clinical Trial

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4625-45.5.4 ◽

2021 ◽

Vol 45 (5) ◽

pp. 312-316

Author(s):

Mishra Neha Sanjeev ◽

Harsimran Kaur ◽

Sandeep Singh Mayall ◽

Rishika ◽

Ramakrishna Yeluri

Keyword(s):

Test Group ◽

Primary Molars ◽

Control Group ◽

Chi Square ◽

Significance Level ◽

Significant Difference ◽

Chi Square Test ◽

Group A ◽

Group B ◽

And Control

Objective: To evaluate the effectiveness of placing a resorbable collagen barrier in impeding the extrusion of obturation material in primary molars undergoing resorption. Study design: All the 94 canals in 47 mandibular molars were allocated to 2 groups- Group ‘A’- 47 canals with collagen barrier (Test group) and Group ‘B’- 47 canals without collagen barrier (Control group) based on randomization protocol. Pulpectomy was performed and obturation of both test and control canals were radiographically assessed. Pearson’s chi – square test was applied to analyze the results. The significance level was predetermined at p < 0.05. Results: Among the test group, 93.6% of the canals showed no extrusion while, 6.4% showed visible extrusion of the material outside the apex. In the control group, 83% showed no extrusion whereas 17% of the canals showed visible extrusion outside the apex. But no significant difference was noted (p>0.05). Conclusion: The placement of resorbable collagen barrier in the apical third of the canal prevented the extrusion of obturating material beyond the apex in resorbing primary molars.

Download Full-text

HbA1c levels in individuals heterozygous for hemoglobin variants

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.63.04.341 ◽

2017 ◽

Vol 63 (4) ◽

pp. 341-346

Author(s):

Ricardo Silva Tavares ◽

Fábio Oliveira de Souza ◽

Isabel Cristina Carvalho Medeiros Francescantonio ◽

Weslley Carvalho Soares ◽

Mauro Meira Mesquita

Keyword(s):

Test Group ◽

Exchange Chromatography ◽

Group Method ◽

Control Group ◽

Significant Difference ◽

Hemoglobin Variants ◽

Different Populations ◽

And Control ◽

Two Populations ◽

Hba1c Levels

Summary Objective: To evaluate the levels of glycated hemoglobin (HbA1c) in patients heterozygous for hemoglobin variants and compare the results of this test with those of a control group. Method: This was an experimental study based on the comparison of HbA1c tests in two different populations, with a test group represented by individuals heterozygous for hemoglobin variants (AS and AC) and a control group consisting of people with electrophoretic profile AA. The two populations were required to meet the following inclusion criteria: Normal levels of fasting glucose, hemoglobin, urea and triglycerides, bilirubin > 20 mg/dL and non-use of acetylsalicylic acid. 50 heterozygous subjects and 50 controls were evaluated between August 2013 and May 2014. The comparison of HbA1c levels between heterozygous individuals and control subjects was performed based on standard deviation, mean and G-Test. Results: The study assessed a test group and a control group, both with 39 adults and 11 children. The mean among heterozygous adults for HbA1c was 5.0%, while the control group showed a rate of 5.74%. Heterozygous children presented mean HbA1c at 5.11%, while the controls were at 5.78%. G-Test yielded p=0.93 for children and p=0.89 for adults. Conclusion: Our study evaluated HbA1c using ion exchange chromatography resins, and the patients heterozygous for hemoglobin variants showed no significant difference from the control group.

Download Full-text

Examination of Haematotoxicity of Fixed-Dose Highly Active Antiretroviral Drug in Albino Wistar Rats

ISRN Pharmacology ◽

10.5402/2012/309084 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6

Author(s):

Thomas Nubila ◽

Ernest O. Ukaejiofo ◽

Nkoyo I. Nubila ◽

Godfrey I. Okorie

Keyword(s):

Highly Active Antiretroviral Therapy ◽

Wistar Rats ◽

Venous Blood ◽

Haemoglobin Concentration ◽

Test Group ◽

Mean Value ◽

Fixed Dose ◽

Control Group ◽

Highly Active ◽

Significant Difference

Highly active antiretroviral therapy (HAART) is considered toxic and has other life-threatening side effects. Our aim was to evaluate the haematotoxic effects of lamivudine, zidovudine, and nevirapine fixed-dose combinations in Albino Wistar rats. Fifty (50) three (3) months old male Albino Wistar rats weighing between 200 and 250 g were randomly assigned to five (5) groups (A, B, C, D, and E). Group A served as control. Two (2 mLs) of venous blood was aseptically collected on Days 5, 10, 15, 20, and 25 of treatment. Red blood cell (RBC) mean value recorded statistically significant increase () in groups B and C when compared with the control group on Day 5. However, there was a statistically significant decrease () in RBC, haemoglobin concentration (Hb), packed cell volume (PCV), and some red cell indices on Day 10. In addition there was no statistically significant difference () in all the parameters evaluated when the test group was compared with the control on Day 25. Furthermore, there was a time-related statistically significant increase () in the two major blood cells—RBC and platelet counts. From the result of this present study, it can be concluded that HAART when administered in fixed-dose combinations have no subacute haematotoxic effects.

Download Full-text

The Impact of Chlorhexidine Mouth Rinse on the Bond Strength of Polycarbonate Orthodontic Brackets

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1600 ◽

2014 ◽

Vol 15 (6) ◽

pp. 688-692 ◽

Cited By ~ 1

Author(s):

Sukumaran Anil ◽

Farouk Ahmed Hussein ◽

Mohammed Ibrahim Hashem ◽

Elna P Chalisserry

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Testing Machine ◽

Test Group ◽

Orthodontic Brackets ◽

Control Group ◽

Mouth Rinse ◽

Significant Difference ◽

Bond Strengths ◽

The Impact

ABSTRACT Objective The purpose of the current in-vivo study was to assess the effect of using 0.12% chlorhexidine (CHX) mouth rinse, before bonding, on shear bond strength of polycarbonate brackets bonded with composite adhesive. Subjects and methods Eighteen orthodontic patients with a mean age 21.41 ± 1.2 years, who were scheduled to have 2 or more first premolars extracted, were included in this study. Patients were referred for an oral prophylaxis program which included, in part, the use of a mouth rinse. Patients were divided into 2 groups, a test group of 9 patients who used 0.12% CHX gluconate mouth rinse twice daily and a control group of 9 patients who used a mouth rinse without CHX, but with same color. After 1 week, polycarbonate brackets were bonded to first premolars with Transbond XT composite adhesive. Premolars were extracted after 28 days and tested for shear bond strength on a universal testing machine. Student's t-test was used to compare shear bond strengths of both groups. Results No statistically significant difference was found in bond strengths’ values between both groups. The test group (with CHX) has mean shear bond strength of 14.21 ± 2.42 MPa whereas the control group (without CHX) revealed a mean strength of 14.52 ± 2.31 MPa. Conclusion The use of 0.12% CHX mouth rinse, for one week before bonding, did not affect the shear bond strength of polycarbonate brackets bonded with Transbond composite. Furthermore, these brackets showed clinically acceptable bond strength. How to cite this article Hussein FA, Hashem MI, Chalisserry EP, Anil S. The Impact of Chlorhexidine Mouth Rinse on the Bond Strength of Polycarbonate Orthodontic Brackets. J Contemp Dent Pract 2014;15(6):688-692.

Download Full-text

Effects of Conventional Synthetic Disease-Modifying Antirheumatic Drugs on Response to Periodontal Treatment in Patients with Rheumatoid Arthritis

BioMed Research International ◽

10.1155/2018/1465402 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Gyu-Un Jung ◽

Ji-Young Han ◽

Kyung-Gyun Hwang ◽

Chang-Joo Park ◽

Panagiota G. Stathopoulou ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Inflammatory Diseases ◽

Periodontal Treatment ◽

Control Group ◽

Scaling And Root Planing ◽

Disease Modifying Antirheumatic Drugs ◽

Antirheumatic Drugs ◽

Root Planing ◽

Significant Difference ◽

Disease Modifying

Rheumatoid arthritis (RA) and periodontitis are common chronic inflammatory diseases and periodontitis is known to be more common and more severe in patients with RA. Based on a paucity of studies about the relationship between common conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) and periodontitis, this prospective study aimed to evaluate the adjunctive effect of csDMARDs on response to nonsurgical periodontal treatment in patients with RA. Thirty-two patients with RA (RA group) and 32 systemically healthy patients (control group) with periodontitis were included in this study. The RA group patients were treated with csDMARDs, such as methotrexate, hydroxychloroquine, and sulfasalazine. Conventional nonsurgical periodontal treatment with scaling and root planing was performed in both groups. The extent and severity of periodontitis were evaluated by plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP) at baseline and 4 weeks after periodontal treatment. There was no statistically significant difference of periodontal parameters between the RA and control groups at baseline. Four weeks after scaling and root planing, PD reduction, and CAL gain were higher in the RA group treated with csDMARDs compared to the control group, and the difference was statistically significant (P = 0.006 and 0.003, respectively). A post hoc analysis of the RA group showed no statistically significant difference on the response to nonsurgical periodontal treatment in multiple csDMARDs therapy and addition of NSAIDs and/or steroids to csDMARDs. In patients with RA, csDMARDs showed beneficial effect on periodontal clinical parameters following the nonsurgical periodontal treatment.

Download Full-text