scholarly journals Morphological and ultrastructural characteristics of the tongue of wild boar

2020 ◽  
Vol 64 (2) ◽  
Author(s):  
Gabriela de Souza Reginato ◽  
Gabriela Klein Barbosa ◽  
Amanda Olivotti Ferreira ◽  
Bruno Gomes Vasconcelos ◽  
Rose Eli Grassi Rici ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Wild Boar ◽  
Structural Characteristics ◽  
Morphological Characteristics ◽  
Taste Buds ◽  
Flower Bud ◽  
Fungiform Papilla ◽  
Lingual Epithelium ◽  
Lingual Papillae ◽  
Conical Shape

The present study aimed to describe the structural and ultrastructural morphological characteristics of the lingual epithelium and the connective tissue cores (CTCs) of wild boar (Sus scrofa). The tongues were processed for light microscopy, scanning electron microscopy, and transmission electron microscopy. In this study, we revealed the filiform, fungiform, foliate, and vallate papillae. The filiform papilla is elongated with a conical shape and its CTC has a conical shape; the fungiform papilla is rounded with a dome-shape and its CTC is flower bud; the foliate papilla is formed by four pairs of epithelial folds and irregular grooves, and its CTC is thin with adjacent conjunctive projections, and taste buds and serous glands in the epithelial layer have been evidenced; and the vallate papilla is oval surrounded by a groove with increases of epithelium surface, and the CTC is formed by numerous connective projections lined. Also noted were serous gland and taste buds on the medial wall of the vallate papilla. The epithelium has the keratinized, granular, spinous, basal, and lamina propria layers. In conclusion, we found new descriptions and shapes of the CTCs of the lingual papillae. In addition, we demonstrated the epithelium structural characteristics, the nuclear distribution between the epithelial layers, and the ultrastructural aspects of the dorsal epithelium of the tongue.

scholarly journals Obtention and Characterization of Ferrous Chloride FeCl2·4H2O from Pickling Waste Liquors

2021 ◽  
Author(s):  
Lorena Alcaraz ◽  
Belén Sotillo ◽  
José F. Marco ◽  
Francisco J. Alguacil ◽  
Paloma Fernández ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Structural Characteristics ◽  
Morphological Characteristics ◽  
Aged Sample ◽  
X Ray Diffraction ◽  
Ferrous Chloride ◽  
Photoluminescence Study ◽  
X Ray ◽  
Scanning Electron

As a hazardous waste, pickling waste liquor must be properly treated. An alternative consists in promoting the formation of ferrous salts from this residue, due to its higher ferrous content. Since FeCl2·4H2O is widely used in several applications, obtaining pure crystals of this material appears to be an interesting prospect. However, this compound has scarcely been investigated. In the present work, FeCl2·4H2O crystals have been obtained from pickling waste liquors. Their structural and morphological characteristics have been investigated by X-ray diffraction, scanning electron microscopy as well as Mössbauer spectroscopy. In addition, the photoluminescence study of the obtained samples was also assessed. It was observed that after some aging time, the obtained crystals change in colour from green to more yellowish. So, the aged sample has also been evaluated, and their structural characteristics are compared with the original crystals. Despite this, the obtained crystals exhibit a FeCl2·4H2O structure, which is not modified with the aging of the sample.

A High Resolution Study of G.P. Zones in Aluminum - 4.2 at % Silver

1982 ◽  
Vol 40 ◽  
pp. 722-723 ◽  
Author(s):  
R. Gronsky
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Computer Simulations ◽  
Structural Characteristics ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Resolution Study

The phenomenon of clustering in Al-Ag alloys has been extensively studied since the early work of Guinierl, wherein the pre-precipitation state was characterized as an assembly of spherical, ordered, silver-rich G.P. zones. Subsequent x-ray and TEM investigations yielded results in general agreement with this model. However, serious discrepancies were later revealed by the detailed x-ray diffraction - based computer simulations of Gragg and Cohen, i.e., the silver-rich clusters were instead octahedral in shape and fully disordered, atleast below 170°C. The object of the present investigation is to examine directly the structural characteristics of G.P. zones in Al-Ag by high resolution transmission electron microscopy.

Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

1972 ◽  
Vol 30 ◽  
pp. 286-287
Author(s):  
N. Savage ◽  
A. Hackett
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Leukemia Virus ◽  
Morphological Characteristics ◽  
Virus Production ◽  
Oncogenic Viruses ◽  
Endogenous Virus ◽  
Virus Particles ◽  
Moloney Leukemia Virus ◽  
A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

scholarly journals A Thermally Conductive Pt/AAO Catalyst for Hydrogen Passive Autocatalytic Recombination

Catalysts ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 491
Author(s):  
Alina E. Kozhukhova ◽  
Stephanus P. du Preez ◽  
Aleksander A. Malakhov ◽  
Dmitri G. Bessarabov
Keyword(s):  
Electron Microscopy ◽  
Particle Size ◽  
Combustion Temperature ◽  
Volume Fraction ◽  
Morphological Characteristics ◽  
Al Alloy ◽  
Impregnation Method ◽  
Case Scenario ◽  
Worst Case ◽  
Thermal Distribution

In this study, a Pt/anodized aluminum oxide (AAO) catalyst was prepared by the anodization of an Al alloy (Al6082, 97.5% Al), followed by the incorporation of Pt via an incipient wet impregnation method. Then, the Pt/AAO catalyst was evaluated for autocatalytic hydrogen recombination. The Pt/AAO catalyst’s morphological characteristics were determined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The average Pt particle size was determined to be 3.0 ± 0.6 nm. This Pt/AAO catalyst was tested for the combustion of lean hydrogen (0.5–4 vol% H2 in the air) in a recombiner section testing station. The thermal distribution throughout the catalytic surface was investigated at 3 vol% hydrogen (H2) using an infrared camera. The Al/AAO system had a high thermal conductivity, which prevents the formation of hotspots (areas where localized surface temperature is higher than an average temperature across the entire catalyst surface). In turn, the Pt stability was enhanced during catalytic hydrogen combustion (CHC). A temperature gradient over 70 mm of the Pt/AAO catalyst was 23 °C and 42 °C for catalysts with uniform and nonuniform (worst-case scenario) Pt distributions. The commercial computational fluid dynamics (CFD) code STAR-CCM+ was used to compare the experimentally observed and numerically simulated thermal distribution of the Pt/AAO catalyst. The effect of the initial H2 volume fraction on the combustion temperature and conversion of H2 was investigated. The activation energy for CHC on the Pt/AAO catalyst was 19.2 kJ/mol. Prolonged CHC was performed to assess the durability (reactive metal stability and catalytic activity) of the Pt/AAO catalyst. A stable combustion temperature of 162.8 ± 8.0 °C was maintained over 530 h of CHC. To confirm that Pt aggregation was avoided, the Pt particle size and distribution were determined by TEM before and after prolonged CHC.

scholarly journals Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus)

ISRN Anatomy ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
A. M. Cruceño ◽  
J. C. de Rosas ◽  
M. Fóscolo ◽  
E. M. Chaves ◽  
L. Scardapane ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Reproductive Cycle ◽  
Sperm Morphology ◽  
Structural Parameters ◽  
Light And Electron Microscopy ◽  
Morphological Characteristics ◽  
Activity Period ◽  
Epididymal Sperm ◽  
Wild Rodent ◽  
Lagostomus Maximus

The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

scholarly journals Approximating deformation fields for the analysis of continuous heterogeneity of biological macromolecules by 3D Zernike polynomials

IUCrJ ◽  
2021 ◽  
Vol 8 (6) ◽  
Author(s):  
David Herreros ◽  
Roy R. Lederman ◽  
James Krieger ◽  
Amaya Jiménez-Moreno ◽  
Marta Martínez ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Structural Characteristics ◽  
Normal Mode Analysis ◽  
Principal Component ◽  
Current Data ◽  
Mode Analysis ◽  
Biological Macromolecules ◽  
Mathematical Basis ◽  
Common Framework ◽  
Low Dimensional

Structural biology has evolved greatly due to the advances introduced in fields like electron microscopy. This image-capturing technique, combined with improved algorithms and current data processing software, allows the recovery of different conformational states of a macromolecule, opening new possibilities for the study of its flexibility and dynamic events. However, the ensemble analysis of these different conformations, and in particular their placement into a common variable space in which the differences and similarities can be easily recognized, is not an easy matter. To simplify the analysis of continuous heterogeneity data, this work proposes a new automatic algorithm that relies on a mathematical basis defined over the sphere to estimate the deformation fields describing conformational transitions among different structures. Thanks to the approximation of these deformation fields, it is possible to describe the forces acting on the molecules due to the presence of different motions. It is also possible to represent and compare several structures in a low-dimensional mapping, which summarizes the structural characteristics of different states. All these analyses are integrated into a common framework, providing the user with the ability to combine them seamlessly. In addition, this new approach is a significant step forward compared with principal component analysis and normal mode analysis of cryo-electron microscopy maps, avoiding the need to select components or modes and producing localized analysis.

scholarly journals Analysis of the type material of Gomphosphenia tackei (Bacillariophyceae) and comparison to epizoic diatom populations on freshwater snails

2021 ◽  
Vol 154 (2) ◽  
pp. 257-263
Author(s):  
Mateusz Rybak ◽  
Łukasz Peszek ◽  
Anita Poradowska
Keyword(s):  
Electron Microscopy ◽  
Lymnaea Stagnalis ◽  
Morphological Characteristics ◽  
Small Cell ◽  
Type Material ◽  
Freshwater Snails ◽  
Sem Images ◽  
Small Cell Size ◽  
Type Population ◽  
Scanning Electron

Background and aims – Hustedt (1942) originally described Gomphosphenia tackei from Germany under the name Gomphonema tackei. Because of the small cell size and the lack of scanning electron microscopy (SEM) images from the type material, it is often confused with other species from this genus, especially with G. stoermeri. The aim of this paper was to present detailed morphological characteristics of G. tackei based on the analysis of the type material and of several epizoic populations from Central Europe. Material and methods – The material in this study was collected from the shells of the freshwater snails Lymnaea stagnalis, Planorbarius corneus, and Planorbis planorbis. Additionally, for an unambiguous species identification, the type material for Gomphosphenia tackei was analyzed using light and scanning electron microscopes.Key results – The presence of Gomphosphenia tackei was confirmed in the studied material. The largest population (up to 19%) was recorded on the shell surfaces of living snails, whereas on empty shells, the diatom did not seem to be present or only in very low numbers. Valves are typically clavate with rounded apices. Valves are frequently observed in girdle view, often joint together in pairs. The valves in the studied populations had a valve length of 7–29 µm, a valve width of 3–4 µm, and a stria density of 25–29 striae in 10 µm. In the type population, valve length ranged from 7.5 to 27 µm with a valve width of 3.0–4.0 µm and a stria density of 23–29 striae per 10 µm. Striae were composed of 2–4 elongated to rounded areolae per stria. At the apices, the striae were composed of one single areola. The cells were attached to the substratum by their footpole.Conclusion – Published illustrations of Gomphosphenia tackei do not always correctly represent this species. Individual cells are attached to the substratum by secreted mucilage, probably via their areolae or girdle band pores located on the footpole.

Four new species of monoraphid diatoms from Western Sichuan Plateau in China

Phytotaxa ◽  
2021 ◽  
Vol 479 (3) ◽  
pp. 257-274
Author(s):  
Q.M. YOU ◽  
K. ZHAO ◽  
Y.L. WANG ◽  
P. YU ◽  
J.P. KOCIOLEK ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
New Species ◽  
Central Area ◽  
Morphological Characteristics ◽  
Similar Species ◽  
Small Species ◽  
Western Sichuan ◽  
The Relationship ◽  
Scanning Electron ◽  
Apical Axis

Four new monoraphid diatoms are examined and described from a high-altitude (> 3000 m) region of western Sichuan, China.  The descriptions are based on light and scanning electron microscopy observations, and the four new species are compared with similar taxa worldwide.  Achnanthidium sichuanense sp. nov. has small, lanceolate-elliptical valves, with rounded apices. The outline and central area differentiate it from similar species.  The unique characteristic of Eucocconeis undulatum sp. nov. is that it has valves that are undulate along the apical axis, forming an “H”-shaped area on both raphe and rapheless valves as seen in light microscope (LM).  Platessa mugecuonesis sp. nov. and Platessa lanceolata sp. nov. are two small species, whose valve outlines and central areas make them different from other species in this genus.  A. sichuanense sp. nov. and E. undulatum sp. nov. are discussed in terms of the relationship between “Achnanthidium & Psammothidium” and “Eucocconeis & Psammothidium”, as these genera are difficult to separate according to morphological characteristics.

CLINICAL AND MORPHOLOGICAL CHARACTERISTICS OF CHRONIC RECURRENT HEMATOGENOUS OSTEOMYELITIS

2020 ◽  
Vol 93 (1) ◽  
pp. 93-99
Author(s):  
Azamat Shamsiev ◽  
◽  
Sukhrob Zainiev ◽  
N. Alieva
Keyword(s):  
Electron Microscopy ◽  
Microbial Contamination ◽  
Morphological Characteristics ◽  
Medullary Canal ◽  
Pathological Changes ◽  
Morphological Studies ◽  
Hematogenous Osteomyelitis ◽  
Transmission Electron ◽  
Bone Fragments ◽  
Tubular Bones

The studied material of scanning and transmission electron microscopy were bone fragments of 40 patients obtained during surgery from various pathologically altered sections of the affected tubular bones. Morphological studies revealed microscopic foci of necrosis, leukocyte infiltrates and microabscesses both in the main focus and in other parts of the bone marrow canal. Microabscesses detected by microscopy represent the morphological basis of chronic hematogenous osteomyelitis, can be located at a considerable distance from the main necrotic purulent focus and, under appropriate conditions, cause a relapse of the disease. Morphological studies have shown that surgical debridement of the lesion with ultrasonic cavitation of the lesion and the medullary canal contributes to a significant reduction in the degree of microbial contamination, followed by a decrease in pathological changes in bone tissue.

Ultrastructural Analysis of Mouthparts of Adult Horn Fly (Diptera: Muscidae) From the Brazilian Midwest Region

2020 ◽  
Vol 57 (5) ◽  
pp. 1447-1458 ◽  
Author(s):  
Fernando de Freitas Fernandes ◽  
Ana Cristina Bahia ◽  
Nágila Francinete Costa Secundino ◽  
Paulo Filemon Paolucci Pimenta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Characteristics ◽  
Sense Organs ◽  
Horn Fly ◽  
Phylogenetic Studies ◽  
Midwest Region ◽  
Well Differentiated ◽  
Maxillary Palps ◽  
Scanning Electron

Abstract The ultrastructure of the mouthparts of Haematobia irritans (L.) was investigated by scanning electron microscopy. The morphological characteristics of the maxillary palps, labium (prementum and postmentum), labrum, hypopharynx, haustellum, and labellar lobes are described, as well as of the sensilla evidenced on all the surface of the mouthparts, and the set of different positions assumed by the mouth apparatus of this fly. Based on their morphology, 12 well-differentiated sensilla were identified, among three types of cuticular sensilla: trichoidea, coeloconica, and campaniformia. A slight sexual dimorphism in the sensilla patterns found in the mouthparts of H. irritans was evidenced. These observations are discussed with reference to the current literature on the functional morphology of sense organs of Insecta. These results could facilitate the recognition of the chemosensory sensilla by electrophysiological techniques, and foment future taxonomic and phylogenetic studies to better elucidate the evolution of Diptera, Muscomorpha.

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