scholarly journals Preliminary results of effect of barley (Hordeum vulgare L.) extract on liver, pancreas, kidneys and cardiac tissues in streptozotocin induced diabetic rats

2021 ◽  
Author(s):  
Mohsen Naseri ◽  
Zahra Khalaj Sereshki ◽  
Behnaz Ghavami ◽  
Bagher Minaii Zangii ◽  
Mohammad Kamalinejad ◽  
...  
Keyword(s):  
Diabetic Control ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Barley Seed ◽  
Histopathological Analysis ◽  
Protective Effects ◽  
Hordeum Vulgare L ◽  
Cardiac Tissues ◽  
Significant Burden ◽  
Male Wistar Rats

Diabetes mellitus (DM) and its complications impose a significant burden on patients and the health care system. In the Traditional Persian Medicine (TPM), barley is recommended for treatment of DM. This study sought to assess the effect of barley seed aqueous extract on hepatic, pancreatic, renal and cardiac tissues in normal (non-diabetic) and Streptozotocin-induced diabetic rats. Twenty-one male Wistar rats were randomly divided into diabetic and non-diabetic groups. Diabetes was induced by single intraperitoneal injection of Streptozotocin. After one week, the diabetic and non-diabetic groups were randomly divided into control and barley seed extract subgroups namely N group (non-diabetic control rats), S group (seed extract treated non-diabetic rats), D group (diabetic control rats) and DS group (seed extract treated diabetic rats). After 6 weeks, all rats were sacrificed for histopathological analysis and specimens were stained routinely for histological studies. The abnormal histological signs significantly decreased in the DS group compared to D group. Also, protective effects of barley seed extract against histopathological changes were seen in S group compared to N group.These findings suggest that barley seed extract exerts a protective effect on different tissues in diabetes.

scholarly journals Physical activity as a non-pharmacological and useful strategy in controlling of the apoptotic symptoms in diabetic model rats

2021 ◽  
Vol 16 (8) ◽  
pp. 602-606
Author(s):  
M. Bostani ◽  
S.A. Noaein
Keyword(s):  
Aerobic Exercise ◽  
Pancreatic Beta Cells ◽  
Diabetic Control ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Aerobic Exercise Training ◽  
Bax Protein ◽  
Male Wistar Rats ◽  
Healthy Control ◽  
Induced Apoptosis

Background. In recent years, diabetes has become a global health problem. Apoptosis of pancreatic beta cells plays an important role in the pathogenesis of type 1 diabetes. Exercise as a non-pharmacological strategy to reduce the diabetic-induced complications has always been of interest to researchers. Therefore, the purpose of this study was to investigate the effect of aerobic exercise on levels of Bax, Bcl-2 and Bax/Bcl-2 ratio in pan­creatic tissue of streptozotocin (STZ)-induced diabetic rats. Materials and methods. A total number of 40 male Wistar rats (10 weeks old, 200–250 gr weight) were randomly divided into healthy control (HC), healthy trained (HT), diabetic control (DC), and diabetic trained (DT) groups. Diabetes was also induced by a single intraperitoneally injection of streptozocin (45 mg/kg). The training groups performed the exercise on the treadmill for five consecutive days within six weeks. The pancreatic tissue levels of the Bax and the Bcl-2 proteins were further determined via ELISA method. Results. The results showed that the induction of diabetes had significantly decreased the levels of Bcl-2 protein and increased the levels of Bax protein and Bax/Bcl-2 ratio in the pancreatic tissue (p < 0.05). As well, the findings showed that six weeks of aerobic exercise training had significantly increased the levels of Bcl-2 and significantly decreased the levels of Bax protein in DT group. Also, the Bax/Bcl-2 ratio reduced significantly in DT group (p < 0.05). The increase in displacement and transmission of apoptosis inducing factor (AIF) that have seen in oxidative stress status, is reduced in the tissues of trained individuals which indica­ting of the inhibition in the apoptotic signaling. Conclusions. According to the results of this study, exercise can be considered as an effective strategy to reduce the rate of diabetic-induced apoptosis and control its complications.

scholarly journals Protective effects of Trigonella foenum-graecum crude extract over damage induced by Sreptozotocin diabetes rats

2020 ◽  
Vol 10 (4-s) ◽  
pp. 138-141
Author(s):  
Dalila Bencheikh ◽  
Seddik Khennouf ◽  
Saliha Dahamna
Keyword(s):  
Streptozotocin Diabetes ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Protective Effects ◽  
Traditional Use ◽  
Orogastric Tube ◽  
Trigonella Foenum Graecum ◽  
Male Wistar Rats ◽  
Treatment Of Diabetes

The seeds of Trigonella foenum-graecum (fenugreek) are used for treatment of diabetes mellitus in traditional medicine. This paper examines the protection effects of fenugreek from the damage induced by streptozotocin diabetes rats. Tannins content of T. foenum-graecum was also estimated in vitro. Normoglycemic male Wistar rats, weighing 170-250 g, were selected and randomly divided into five groups (n= 6): normal control, diabetic + TFGE (200mg/kg), diabetic+ TFGE (600mg/kg), diabetic + Glibil (3mg/kg), untreated group. Diabetes was induced after a single intraperitoneal injection of streptozotocin (50 mg/kg body weight) and Fenugreek was given every day via orogastric tube for 18 days. At the end of experiment, rats were sacarificed. Organ weight was estimated of all groups. Trigonella foenum-graecum administration significantly improved the polydipsia, polyphagia, and it also compensated weight loss of diabetic rats (P<0.05, P < 0.01). Moreover, fenugreek had a significant concentration of tannins (806.22 ±0.036 µg TAE/gE). The results revealed that fenugreek improves the damage in diabetic rats that in some ways validates the traditional use of this plant in treatment of diabetes. Keywords: Antidiabetic activity, Protective effect, Streptozotocin, Tannins, Trigonella foenum-graecum

scholarly journals Hypoglicemic and Hypolipedimic Effects of Ganoderma lucidum in Streptozotocin-Induced Diabetic Rats

Medicines ◽  
2018 ◽  
Vol 5 (3) ◽  
pp. 78 ◽  
Author(s):  
Erna Bach ◽  
Edgar Hi ◽  
Ana Martins ◽  
Paloma Nascimento ◽  
Nilsa Wadt
Keyword(s):  
Ganoderma Lucidum ◽  
Biochemical Analysis ◽  
Food Supplement ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Histopathological Analysis ◽  
Beta Glucan ◽  
Male Wistar Rats ◽  
Normoglycemic Control

Background:Ganoderma lucidum (Leyss. Ex. Fr) Karst is a basidiomycete mushroom that has been used for many years as a food supplement and medicine. In Brazil, National Health Surveillance Agency (ANVISA) classified Ganoderma lucidum as a nutraceutical product. The objective of the present work was to observe the effects of an extract from Ganoderma lucidum in rats treated with streptozotocin, and an agent that induces diabetes. Method: Male Wistar rats were obtained from the animal lodging facilities of both University Nove de Julho (UNINOVE) and Lusiada Universitary Center (UNILUS) with approval from the Ethics Committee for Animal Research. Animals were separated into groups: (1) C: Normoglycemic control water; (2) CE: Normoglycemic control group that received hydroethanolic extract (GWA); (3) DM1 + GWA: Diabetic group that received extract GWA; and (4) DM1: Diabetic group that received water. The treatment was evaluated over a 30-day period. Food and water were weighted, and blood plasma biochemical analysis performed. Results: G. lucidum extract contained beta-glucan, proteins and phenols. Biochemical analysis indicated a decrease of plasma glycemic and lipid levels in DM rats induced with streptozotocin and treated with GWA extract. Histopathological analysis from pancreas of GWA-treated DM animals showed preservation of up to 50% of pancreatic islet total area when compared to the DM control group. In plasma, Kyn was present in diabetic rats, while in treated diabetic rats more Trp was detected. Conclusion: Evaluation from G. lucidum extract in STZ-hyperglycemic rats indicated that the extract possesses hypoglycemic and hypolipidemic activities. Support: Proj. CNPq 474681/201.

scholarly journals The Protective Effect of Marigold Hydroalcoholic Extract in STZ-Induced Diabetic Rats: Evaluation of Cardiac and Pancreatic Biomarkers in the Serum

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Esmaeel Ebrahimi ◽  
Saeed Shirali ◽  
Rahman Talaei
Keyword(s):  
Diabetes Mellitus ◽  
Protective Effect ◽  
Antioxidant Property ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Diabetes mellitus is the most common endocrine disorder worldwide and it is usually along with complications such as retinopathy, neuropathy, nephropathy, and cardiovascular disease. The prevalence of diabetes is increasing and its treatment has created concerns in society. The use of herbal medicine can be helpful in the management of diabetes mellitus. The aim of this study was to investigate the protective effect of marigold hydroalcoholic extract under diabetes condition. A total of 36 male Wistar rats were randomly divided into four groups: normal control group, diabetic control group, and groups treated with 250 or 500 mg/kg hydroalcoholic extract of marigold flower during four weeks. At the end of the study, the rats were anesthetized with ketamine/xylazine, and sampling was performed through cardiac puncture. The results showed that treatment with marigold improved body weight. In addition, we determined that marigold normalized the level of CK-MB, total CK, amylase, and lipase in a dose-dependent manner. Probably these effects resulted from antioxidant property of marigold; thus we suggest that marigold flower can be useful for reduction of diabetes complication.

scholarly journals Effect of the Combination of Training and ERRα Inhibition on Liver Metabolism by Modulation of PDK4 and LXR-α Expression in STZ-Induced Diabetic and Healthy Rats

2020 ◽  
Vol 10 (6) ◽  
pp. 7011-7022 ◽  
Keyword(s):  
Exercise Training ◽  
Endurance Training ◽  
Dietary Habits ◽  
Metabolic Diseases ◽  
Life Quality ◽  
Public Health Problem ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Male Wistar Rats

Diabetes is a public health problem that affects life quality. Exercise training (ET) and controlled dietary habits improve metabolic diseases such as diabetes. The mechanisms by which exercise training ameliorate metabolic diseases are not fully clear. We designed the current study to evaluate the combination of ERRα suppression and ET effects on the expression of LXR-α, PDK4, and PPARα in healthy and STZ-induced diabetic rats. Fifty-six male Wistar rats were divided into 8 groups (n = 7) as follows; control, diabetic control (a single dose of 45 mg/kg of STZ), ERRα inhibition group (received 0.48 mg/kg of XCT790), endurance training, diabetic rats which received XCT790, diabetic rats which performed endurance training, rats which received XCT790 and performed endurance training, and diabetic rats which received XCT790 and also performed endurance training. Expression of the target gene and protein was carried out on the liver tissue. Our results showed that ET significantly increased PDK4, PPARα, and ERRα expression. ERRα suppression significantly increased LXR-α and PDK4 expression in healthy rats compared to the healthy control group. In the diabetic group with ERRα suppression, LXR-α expression significantly upregulated. The combination of ET and ERRα suppression did not change LXR-α expression compared to healthy and diabetic groups (CTL/ERR), but the expression of PDK4, PPARα, and ERRα was significantly upregulated.

scholarly journals Protein Isolates from Fermented Melon Seeds Promoted Differential Expression in Liver and Pancreas of Streptozotocin Induced Diabetic Male Wistar Rats

2020 ◽  
pp. 6-18
Author(s):  
A. O. Iyoriobhe ◽  
A. O. Abiola ◽  
P. A. Adedigba ◽  
P. H. Fagbenro ◽  
T. A. Adebisi ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Protein Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Protein Isolates ◽  
Male Wistar Rats ◽  
Melon Seeds

Diabetes mellitus (DM) is the most common endocrine disorder of human. However, the anti-diabetic activity of protein isolates from fermented plants seed for DM remains enigmatic. The prevalence of diabetes in Africa is exponentially increasing with more deaths occurring directly from diabetes mellitus or from its associated complications. The current study investigated the effect of Protein isolate from fermented melon seeds (Ogiri; OPI) of Cucumeropsis manni on blood glucose, hepatic and pancreatic protein profile, histopathological parameters, identification and characterisation of expressed proteins in streptozotocin (STZ)-induced diabetic rats. Thirty Male wistar rats were divided into nondiabetic control, STZ-diabetic control, STZ-Ogiri protein isolate supplemented group (STZ-OPI; 200 mg/kg diet), STZ-Ogiri protein isolate supplemented group (STZ-OPI; 600 g/kg diet) and STZ-glibenclamide treated group (STZ-GBN; 0.5 mg/kg diet). Diabetes was induced by a single injection of STZ (60 mg/kg BW) freshly dissolved in 0.1 mol/L citrate buffer (pH 4.5) into the intraperitonium. Diabetes was confirmed by measuring the fasting blood glucose concentration 48-h post-injection. The rats with blood glucose level above 290 mg/dL were considered to be diabetic. Ogiri protein isolates was supplemented in the diet for 6 weeks. The supplementation OPI reduced (P< 0.05) the blood glucose concentration of the STZ-induced diabetic rats. OPI supplemented groups had significantly higher percentage body weight gain. The high dose OPI supplemented group had a lowest liver protein concentration (19.39 mg/dl) but a significantly (P< 0.05) higher pancreas protein concentration when compared to all the diabetic control. Histological sections of examined tissue revealed accumulation of fat in the liver of diabetic rats and necrosis of the islet of Langerhans were observed in the pancreas. 1DE SDS-PAGE of hepatic and pancreatic tissue homogenates revealed differential expression of 150 kDa proteins in rats treated with 200 mg/kg body weight of OPI only and 20-25 kDa proteins in rats treated with 600 mg/kg body weight of OPI respectively. This result show that OPI supplementation may impose a direct or indirect inhibitory or post translational modification on specific proteins implicated in hyperglycemia and diabetes and as such be a potential antihyperglycemic agent in the management of diabetes.

scholarly journals Extract Ethanol of Poguntano in Alloxan Induced Diabetic Rats

2018 ◽  
Vol 17 (2) ◽  
pp. 251-254
Author(s):  
Sry Suryani Widjaja ◽  
Rusdiana
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Medical Science ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
High Blood Glucose ◽  
Endothelin 1 ◽  
Back Ground ◽  
Male Wistar Rats

Back Ground: Diabetes mellitus is a group of metabolic disease with high blood glucose level above 150 mg/dL over a prolonged period. The elevated of blood glucose, free fatty acid and insulin resistance will cause the endothelial dysfunction, hemostasis disturbances that lead to micro and macrovascular complications. Poguntano (Picria fel-terrae Merr) from family Scrophulariaceae found in most part of Indonesia, has been used as traditional plant for treatment of diabetes.Objective: The purpose of this study was to investigate the effect of extract ethanol of Poguntano in alloxan induced diabetic rats.Method: Fifteen male Wistar rats with body weight of 150-200 gr were given intra peritoneal injection of 150 mg/kg Alloxan to induce diabetes. These were divided into three groups (control diabetic, group given insulin and group given Extract ethanol of Poguntano 200 mg/Kg body weight) and one control normal control. The duration of study was 4 weeks; blood glucose and Endothelin-1 were measured for all groups.Results : Extract ethanol of Poguntano 200 mg showed significant results (p<0.001) in lowering blood glucose in Alloxan induced diabetic rats compared to control diabetic, but did not show superior to insulin group (p=0.892). Endothelin-1 showed statistical significant between group with normal rats and diabetic control rats. (p=0.009) but did not in the other groups. Levels of Endothelin-1 was higher in the diabetic control group with the median 1.95 (1,78- 3.1).Conclusion: In our study we found that extract ethanol of Poguntano showed significant hypoglycemic activity and lowering the Endothelin-1 level in alloxan induced diabetic rats.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.251-254

scholarly journals ANTIDIABETIC EFFECT OF MIKANIA SCANDENS ON ALLOXAN-INDUCED RAT MODEL

2018 ◽  
Vol 11 (3) ◽  
pp. 109
Author(s):  
Rimashree Baishya ◽  
Anjan Adhikari ◽  
Sharmistha Biswas ◽  
Soma Banerjee
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Standard Drug ◽  
Glucose Levels ◽  
Male Wistar Rats ◽  
Histopathological Studies ◽  
Alloxan Monohydrate

 Objective: The objective was to study the evaluation of antidiabetic and antioxidant activity of different doses ethanolic extract of Mikania scandens leaves.Methods: The rats were divided into five groups of six animals each (non-diabetic control, diabetic control, standard drug-treated, and plant treated groups (250 and 500 mg/kg body weight)). Diabetes was induced in the healthy male Wistar rats (150–200 g body weight, 4–6 weeks old) by the administration of alloxan monohydrate (150 mg/kg, i.p.). On the 0th, 3th, 7th, 11th, and 15th days, the blood samples were analyzed for blood glucose. The antioxidant and antidiabetic parameters were evaluated by standard protocol. The liver tissue was used for histopathological assessment of liver damage.Results: The ethanolic extract of leaves of M. scandens showed a significant reduction (p<0.001) in the blood glucose levels and the antioxidant levels, with 500 mg/kg of body weight in the alloxan-induced diabetic rats as compared to the controls. Histopathological studies provided evidence that the leaf extract possesses antidiabetic activity.Conclusions: The ethanolic extract of leaves of M. scandens showed potent antidiabetic and antioxidant abilities against alloxan-induced rats, which indicate the presence of hypoglycemic constituents in the plant.

Protective effect of hydro-alcoholic extract of achillea millefolium on renal injury and biochemical factors in streptozotocin-induced diabetic rats

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Arash Karimi ◽  
Hamid Reza Niazkar ◽  
Pouria Sefidmooye Azar ◽  
Helda Tutunchi ◽  
Mozhde Karimi ◽  
...  
Keyword(s):  
Mrna Level ◽  
Kidney Tissue ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Achillea Millefolium ◽  
Control Group ◽  
Alcoholic Extract ◽  
Content Type ◽  
Management Of Complications ◽  
Male Wistar Rats

Purpose Herbal medicine has been used for the management of complications of diabetes. The purpose of this paper is to examine the anti-diabetic effects of Achillea millefolium extract on diabetic rats. Design/methodology/approach To this aim, 32 male Wistar rats were randomly assigned into four groups in which each group comprised eight rats. The four experimental groups were as follows: control group, diabetic control (DC) group (STZ; 50 mg/kg), diabetic rats, receiving 250 mg/kg hydro-alcoholic extract of the A. millefolium (DAM) and diabetic rats, receiving 5 mg/kg glibenclamide (DG). After 21 days of the treatment course, tissues of the kidney and blood samples were collected for histopathological, biochemical and molecular analysis. Findings The concentration of malondialdehyde (MDA) and glucose serum were markedly reduced in the DC group while significantly increased in DG and DAM groups (1.11 ± 0.57 to 19.4 ± 3.5 and 17.8 ± 1.2 p = 0.002 and 325 ± 0.18 to 223 ± 0.11 and 211 ± 0.32 p = 0.02, respectively). Also, the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were markedly reduced in the DC group while significantly increased in DAM and DG groups (9.1 ± 2.21 to 18.7 ± 3.81 and 14.9 ± 3.1 p = 0.03 and p = 0.02, respectively). The concentrations of creatinine, blood urea nitrogen (BUN) and urea were substantially decreased in DAM and DG groups as compared with the DC group (0.49 ± 0.02 to 0.27 ± 0.01 and 0.25 ± 0.01 p = 0.01, 15.6 ± 2.1 to 7.2 ± 0.68 and 8.6 ± 1.2 p = 0.02 and 114 ± 9.4 to 59.8 ± 5.2 and 64 ± 5.2 p = 0.03, respectively). Also, Bcl-2-associated X protein (BAX) expression was significantly decreased in DAM and DG groups as compared with the DC group (1.3 ± 0.32 to 0.91 ± 0.03 and 0.93 ± 0.02 p = <0.01) and Bcl-2 expression were significantly increased in DAM and DG groups as compared with the DC group (0.42 ± 0.05 to 0.88 ± 0.07 and 0.85 ± 0.06 p = 0.01). Originality/value Diabetes led to degenerative damages in the kidney of rats and increased the mRNA level of Bax, while treatment with A. millefolium could protect the kidney tissue against diabetes complications and increased the mRNA expression of Bcl-2. This study indicated that A. millefolium extracts not only improves renal function as a result of anti-oxidant activity but also modulates some biochemical factors in diabetic rats.

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