Estrogenic activity of a hydro-alcoholic extract of Bambusa arundinaceae leaves on female wistar rats

Objective: The present study was formulated in order to Evaluate the Antifertility Potential of Ficus Racemosa Linn on Female Wistar Rats.Methods: Effects of Ficus Racemosa bark extract was studied on physicochemical parameters, successive solvent extraction and phytochemical screening and antifertility activity. After acute oral toxicity study, antifertility activity in proven fertile female Wistar Rats at the doses 500mg/kg b.wt./day for 30 days. Effects on dimensions of Reproductive outcome, Anti-implantation, Abortifacient, Estrogenic and Antiestrogenic activity were observed.Results: Phytochemical studies of Ficus Racemosa bark shown positive test for Alkaloids, Steroid, Flavonoids, Terpene, Carbohydrates and Tannin. The extract of Ficus Racemosa has anti-fertility effect the control rats showed good number of litters. Treatment of animal with different extracts, resulted a significant (P< 0.05, P< 0.01). antifertility activity (56.5% and 40.3%) was exhibited by AFR and WFR respectively. After 21 days of the extracts free period, the antifertility effect of the extracts was reversed. The extract treatments with AFR, an increase in the resorption index (%) by the extract is an indication of failure in the development of the embryo. The mean percentage of anti-implantation and percent resorption (abortifacient) were found to be highest for AFR-41.21%, WFR 28.07, and AFR-32.56%, WFR-20.76% respectively. The decrement in implantation caused by the extracts may be due to estrogenic or anti-estrogenic activity. However, along with standard AFR exhibiting more potent estrogenic and less potent anti-estrogenic when compared with standard.Conclusion: The above results revealed the potential, reversible Female antifertility effect of alcoholic extract F. Racemosa bark.

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Estrogenic Effect of Bambusa arundinacea, Trichosanthes dioica and Punica granatum on Rats

Indian Journal of Animal Research ◽

10.18805/ijar.b-4760 ◽

2022 ◽

Author(s):

Akanksha Awasthi ◽

Mamta F. Singh ◽

Saurabh Sharma

Keyword(s):

Wistar Rats ◽

Estrogenic Activity ◽

Ethanolic Extract ◽

Punica Granatum ◽

Mature Female ◽

Female Rats ◽

Vaginal Opening ◽

Estrogenic Effect ◽

Female Wistar Rats ◽

Trichosanthes Dioica

Background: Phytoestrogens have recently become a hot topic among scientists. Phytoestrogens’ estrogen-like properties have led to their widespread use in the reproductive system. The aim of this research was to see whether the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum had any estrogenic activity in female wistar rats. Methods: In female wistar rats, the estrogenic effect was studied using a uterotropic assay, vaginal cytology and vaginal opening. In ovariectomized immature and mature female wistar rats, a 400 mg/kg body weight (b.w.) dose of ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum was given. Result: When compared to ovariectomized control rats, the uterine wet weight increased significantly. The estrogen-treated rats had only cornified epithelial cells, indicating the existence of oestrogen, as well as 100% vaginal opening. At 400 mg/kg b.w., the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum demonstrated promising estrogenic activity, as evidenced by uterotropic assays, vaginal opening measurements and histopathological changes. As a result of this research, it’s possible to infer that the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum play an important role in estrogenic activity in female rats.

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Pharmacological Evaluation and Antifertility Activity of Aloe barbadensis Linn on female wistar rats.

International Journal of Phytomedicine ◽

10.5138/09750185.1931 ◽

2017 ◽

Vol 9 (2) ◽

Cited By ~ 1

Author(s):

Sunil Kumar Shah ◽

Deenanath Jhade ◽

Rajendra Chouksey

Keyword(s):

Wistar Rats ◽

Estrogenic Activity ◽

Aloe Vera ◽

Reproductive Physiology ◽

Aloe Barbadensis ◽

Fertile Female ◽

Fertility Effect ◽

Female Wistar Rats ◽

The Mean ◽

Good Number

Ethanolic and Aqueous extract of Leaf of Aloe barbadensiswas study, antifertility activity in proven fertile female Wistar Rats at the doses 500mg/kg b.wt./day for 30 days. Different parameters were studied in female wistar rats including effect of Reproductive outcome, Anti-implantation, Abortifacient study and Estrogenic and Anti-estrogenic activity, Phytochemical were observed.,Aloe vera Leaves shown positive test for Alkaloids, Steroid, Flavonoids, Terpene, Carbohydrates and Tannin. The extract of Aloe vera has anti-fertility effect the control rats showed good number of litters. Treatment of animal with different extracts, resulted a significant (P< 0.05, P< 0.01). antifertility activity (42.2% and 7.8%) was exhibited by AAV and WAV respectively. After 21 days of the extracts free period, the antifertility effect of the extracts was reversed. The extract treatments with AAV, an increase in the resorption index (%) by the extract is an indication of failure in the development of the embryo. The mean percentage of anti-implantation and percent resorption (abortifacient) were found to be highest for AAV-29.46%, WAV -12.17, and AAV-32.96%, WAV-7.78% respectively. The decrement in implantation caused by the extracts may be due to estrogenic or anti-estrogenic activity. However, along with standard AAVexhibiting more potent estrogenic and less potent anti-estrogenic when compared with standard.Clinical assessment of Female antifertility agents should include acceptability, safety and efficacy during and after the treatment. The present study was therefore carried out to evaluate the claimed antifertility effect of Aloe veraleaf using different aspects of reproductive physiology in female wistar rats.

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Effects of alcoholic extract of Myristica fragrans (nutmeg) on pregnancy outcome in female Wistar rats

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v25i8.31 ◽

2021 ◽

Vol 25 (8) ◽

pp. 1483-1491

Author(s):

C.L. Sakpa ◽

D.O. Eguavoen

Keyword(s):

Wistar Rats ◽

Ovarian Follicles ◽

Control Group ◽

Alcoholic Extract ◽

Alcohol Extract ◽

Orogastric Tube ◽

Group 4 ◽

Myristica Fragrans ◽

Female Wistar Rats ◽

Group 2

Myristica fragrans has been reported to be a medicinal plant as it possesses hepatoprotective, antimicrobial, anti-oxidant, antiulcer, anticancer and immunostimulatory properties, as well as antifertility potentials. This study therefore aimed to investigate the effects of alcoholic extract of M. fragrans on dam size, litter size, pup weight, ovarian follicles, endometrial glands and histopathological indices on pregnancy outcomes in female Wistar rats using standard methods. Pregnancy was induced in forty-eight (48) female Wistar rats that were randomly selected and assigned into four (4) groups of twelve (12) rats each: Group 1 (pregestational phase of pregnancy), Group 2 (germinal phase of pregnancy), Group 3 (embryonic phase of pregnancy) and Group 4 (Fetal phase of pregnancy). The treated animals all received 1500 mg of alcoholic extract of M. fragrans. Administration of distilled water and the extract was by orogastric tube. Data were subjected to statistical analysis using the IBM SPSS statistics software (Statistical Package for Social Science) (Version 25) and relevant statistical values were obtained. The extract prevented the proliferation of ovarian follicles and endometrial glands. In addition, during the pregestational, germinal and embryonic phases, there were significant increases in weight of pups when the treated animals were compared to the control group. The dams also showed decreases in body weight during the embryonic phase. This study therefore demonstrated that alcohol extract of Myristica fragrans may serve as a contraceptive agent in view of its anti-fertility effect.

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nxiolytic Effect of the Hydro-alcoholic Extract of Anethum Graveolens Seed in Adult Female Wistar Rats: Modulation of GABA Receptors

Caspian Journal of Neurological Sciences ◽

10.32598/cjns.8.28.302.1 ◽

2022 ◽

Vol 8 (1) ◽

pp. 1-6

Author(s):

Rana Shahabi ◽

◽

Mohammad Rostampour ◽

Behrooz Khakpour ◽

Bahram Soltani ◽

...

Keyword(s):

Locomotor Activity ◽

Gaba Receptors ◽

Industrial Development ◽

Wistar Rats ◽

Anxiolytic Effect ◽

Control Group ◽

Alcoholic Extract ◽

Gamma Aminobutyric Acid ◽

Anethum Graveolens ◽

Female Wistar Rats

Background: Along with industrial development and the increasing social complexity of societies, anxiety is one of the most prevalent psychological disorders. Medicinal plants are considered as an enrichment source of ingredients with biological activity. Objectives: The aim of this study was to evaluate the anxiolytic effect of Anethum Graveolens seed (AGS) and the possible involvement of Gamma-Aminobutyric Acid (GABA)-ergic system in the AGS effect. Materials & Methods: In the present experimental study, 64 female Wistar rats were divided into eight groups and received various concentrations of hydroalcoholic extract of AGS. To measure the level of anxiety, an elevated plus maze was used in a way that the animal’s head turned to an open arm. Prior to the injections of AGS extract, the GABA receptor antagonist was used. The results were analyzed by one-way analysis of variance using IBM SPSS v. 16. Results: Dose-response experiments showed that the AGS extract significantly decreased the anxiety indices compared to the control group (P<0.05). To analyze locomotor activity, our data showed that AGS extract at 0.1, 1, and 10 mg/kg could significantly increase locomotor activity compared to the control group (P<0.001). Pentylenetetrazol (PTZ (+extract significantly decreased the anxiolytic effect of AGS extract (P<0.01). Conclusion: Considering the anti-anxiety effects of AGS extract and a reduction in this effect caused by PTZ, part of the anti-anxiety effect of extract might be assumed via its interaction with GABA-ergic receptors. Further experimental trials; however, are required for the establishment of the anti-anxiety impact of AGS.

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ANTI-ESTROGENIC PROPERTIES OF ETHANOLIC LEAF EXTRACT OF BOERHAVIA DIFFUSA ON FEMALE WISTAR RATS.

10.1055/s-0037-1608549 ◽

2017 ◽

Author(s):

C Olajide Omolola ◽

J Okpuzor ◽

I Nnadozie Jane ◽

R Adewole Adeniyi

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Female Wistar Rats ◽

Estrogenic Properties ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

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NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0750569 ◽

1974 ◽

Vol 75 (3) ◽

pp. 569-578 ◽

Cited By ~ 29

Author(s):

G. Buffler ◽

S. Roser

Keyword(s):

Wistar Rats ◽

Venous Blood ◽

Oestrous Cycle ◽

Female Rats ◽

Cycle Duration ◽

Follicular Growth ◽

The Third ◽

Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

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