EFFECT OF PROCESSING METHODS ON THE IN VITRO DIGESTIBILITY OF SODIUM HYDROXIDE TREATED ROUGHAGES

1970 ◽  
Vol 50 (3) ◽  
pp. 657-662 ◽  
Author(s):  
B. G. OLOLADE ◽  
D. N. MOWAT ◽  
J. E. WINCH
Keyword(s):  
Sodium Hydroxide ◽  
Dry Matter ◽  
Water Solubility ◽  
Lignin Content ◽  
Barley Straw ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Naoh Treatment

The response of roughages to sodium hydroxide (NaOH) treatment varied with type of roughage. Treatment with NaOH at 23 C for 24 hr increased in vitro dry matter digestibility (IVDMD) by 8,5, 39.6 and 21.5 percentage units for alfalfa stem, barley straw and corn stover, respectively. Increased IVDMD values were brought about, in part, by increased water solubility and decreased cell wall constituents. No significant changes occurred in acid detergent fiber, cellulose or lignin content. The IVDMD values of barley straw ranged from 38% at 0% NaOH to 81% with 12% NaOH at 130 C. At all temperatures and durations, IVDMD increased with increase in concentration of NaOH up to the 8% level. Above 8% NaOH, no further increase in IVDMD occurred. Temperature affected the rate as well as the extent of the response to NaOH. Treatments at 100 C for 90 min resulted in IVDMD values approximately 10 percentage units higher than at 23 C for 24 hr.

RELATIONSHIP BETWEEN STEM DIAMETER AND IN VITRO DIGESTIBILITY OF FORAGES

1967 ◽  
Vol 47 (4) ◽  
pp. 423-426 ◽  
Author(s):  
D. N. Mowat ◽  
R. S. Fulkerson ◽  
E. E. Gamble
Keyword(s):  
Dry Matter ◽  
Lignin Content ◽  
Stem Diameter ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Stem Size ◽  
The Third ◽  
Dry Matter Digestibility ◽  
Flower Stage

Stem diameter of Vernal alfalfa was markedly greater from first cut than from aftermaths, particularly the third cut. Stems were larger from clump than from solid spacings within rows. Only the narrowest row widths tended to influence stem size. When alfalfa was cut at the first-flower stage, stem diameter had no effect on in vitro dry matter digestibility. Furthermore, stem width had no influence on the digestibility of various bromegrass entries cut at the heads-elongated stage. No consistent relationship occurred between stem width and lignin or acid-detergent fiber content with alfalfa. However, the narrow stems of bromegrass had a slightly higher lignin content.

scholarly journals COMPARATIVE CELL WALL CONSTITUENT LEVELS OF TANNIN-FREE AND TANNIN-CONTAINING CULTIVARS OF FABABEANS (VICIA FABA L.)

1978 ◽  
Vol 58 (4) ◽  
pp. 775-781 ◽  
Author(s):  
R. R. MARQUARDT ◽  
J. A. McKIRDY ◽  
A. T. WARD
Keyword(s):  
Cell Wall ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Unit Weight ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Series Of Experiments ◽  
Similar Basis

A series of experiments has been carried out with tannin-free and tannin-containing cultivars of fababeans. The in vitro dry matter digestibility of the cotyledon portion of the two groups of fababeans was the same (88.2%) whereas the in vitro digestibility of the testa portion of the tannin-free cultivars was much higher (27.6%) than that of the tannin-containing cultivars (9.2%). The concentrations of certain cell wall constituents in the testae of tannin-free and tannin-containing cultivars were also different (P < 0.01); the respective average values for the two groups were: condensed tannins, 0.2 and 4.9%; lignin, 4.2 and 6.2%; acid detergent fiber, 72 and 66%; and cellulose, 67 and 60%. There was a negative correlation between the in vitro dry matter digestibility and the amount per unit weight of whole beans of either condensed tannin (r = −0.86, P < 0.01) or lignin (r = −0.89, P < 0.01). When expressed on a similar basis there was no correlation between levels of cellulose or acid detergent fiber and in vitro dry matter digestibility.

Effect of partial delignification on the in vitro digestibility of cell wall polysaccharides in Digitaria decumbens (pangola grass)

1978 ◽  
Vol 29 (6) ◽  
pp. 1157 ◽  
Author(s):  
CW Ford
Keyword(s):  
Dry Matter ◽  
Lignin Content ◽  
In Vitro Digestibility ◽  
Sodium Chlorite ◽  
Cell Wall Polysaccharides ◽  
Dry Matter Digestibility ◽  
Digitaria Decumbens ◽  
Total Digestion ◽  
Two Stages

Leaf and stem fractions of Digitaria decumbens (pangola grass) cut at two stages of regrowth (47 and 89 days), were extracted with ether, ethanol and water (100°C) before being partially delignified with sodium chlorite in acetic acid. Delignification reduced the lignin concentrations by 52 and 72% in the 47-day-old leaf and stem, and by 69 and 87% in the 87-day-old leaf and stem. In vitro dry matter digestibility was correspondingly increased, by 17–20% (47-day material) and 28–31% (89-day material). Hemicelluloses were digested to a greater degree than cellulose in the delignified material. The reverse was found in the material prior to delignification. In the hemicelluloses arabinose was always digested to a greater extent than xylose. Polysaccharides from delignified 89-day regrowth leaf and stem tissue were still digested to a lesser extent than those from the 47-day regrowth. Maximum digestibilities estimated for zero lignin content were 96.6, 88.1 and 82.8% for young leaf, young stem-told leaf, and old stem respectively. Thus, while lignin has been shown to be the major factor limiting total digestion of the plant material, other minor factors could not be excluded, particularly in the older tissues.

scholarly journals Fermentative profile and nutritional value of olive bagasse silage with feed additives

2020 ◽  
Vol 36 (1) ◽  
Author(s):  
Neliton Flores Kasper ◽  
Gabriela Ceratti Hoch ◽  
Othon Dalla Colletta Altermann ◽  
Fabiane Quevedo Da Rosa ◽  
Leonardo Ereno Tadielo ◽  
...  
Keyword(s):  
Organic Matter ◽  
Rice Bran ◽  
Dry Matter ◽  
Feed Additives ◽  
Neutral Detergent Fiber ◽  
Mineral Matter ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Dry Matter Digestibility

The aim of this study was to measure the chemical composition, microbiological profile, fermentative characteristics and the aerobic stability of the olive bagasse silages in natura and added with corn bran, soybean and rice bran in different times of sampling. The was completely randomized design in arrangement of plots subdivided in 4x3 time, with five replications. In the plots were allocated the main treatments, and in the subplots the sampling times were allocated. The fermentative characteristics was studied by determination of the dry matter (DM) content, pH and ammoniacal nitrogen (NH3-N), the microbiological by determining the populations of filamentous fungi, Clostridia, lactic acid bacteria and enterobacteria. In the nutrient profile study, the contents of mineral matter (MM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, cellulose, hemicellulose, nitrogen bound to acid detergent fiber (NIDA), nitrogen bound to neutral detergent fiber (NIDN), carbohydrate and total digestible nutrient (TDN). At the ensilage moment, it also has been determined in vitro dry matter digestibility (IVDMD) and in vitro digestibility of organic matter (IVDOM). The use of corn and rice bran provided a better fermentative profile in the studied ensilage. The pH of the silages added corn and rice bran has presented in 4.00 and 4.06 after 112 storage days, consequently. The adding of soybean bran provided the greatest CP values and non-fibrous carbohydrates (NFC) after the fermentative period, been it 131.55 g kg-1 of DM for CP and 176.28 g kg-1 of DM for NFC. The treatments without bran adding and rice bran added have demonstrated IVDOM levels of 581.12 g ka-1 od DM and 604.51 g kg-1 of DM, consequently. The studied meals improve the nutritional profile of the studied silages and are potentially usable as additives in olive bagasse silages.

scholarly journals Chemical Composition and Digestibility of Tropical Grasses

1969 ◽  
Vol 60 (2) ◽  
pp. 186-200
Author(s):  
H. Kayongo-Male ◽  
J. W. Thomas ◽  
D. E. Ullrey ◽  
R. J. Deans ◽  
J. A. Arroyo-Aguilú
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Lignin Content ◽  
Correlation Coefficients ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Predictive Equations ◽  
Tropical Grasses

One hundred and one tropical grasses, lightly fertilized with ammonium nitrate at the rate of 350 kg/ha, were harvested by hand (machete) at 30 days of growth. Crude protein content ranged from 9.8 to 23.8% while lignin content ranged from 2.6 to 7.9%. Wide ranges in the percentage of neutral-detergent fiber (45.7 to 79.2%), acid-detergent fiber (30.9 to 45.3%), hemicellulose (11.7 to 37.5%), and silica (0.4 to 5.5%) were obtained. Digestibility estimates obtained by the Tilley and Terry method and neutral-detergent fiber digestibilities had wide ranges: 42.6 to 66.0% and 22.0 to 62.0%, respectively. Total dry matter disappearance ranged from 44.3 to 78.2%. Digestibility estimates from predictive equations ranged from 74.0 to 94.5% for estimated digestible dry matter, 67.3 to 91.7% for estimated true digestible dry matter, and 54.4 to 78.8% for estimated apparent digestible dry matter. Analysis of variance showed significant variations between and within genera, indicating a possibility of genetic improvement through selection for high nutritive value. Silica was variably correlated with digestibility depending on genera. Acid-detergent fiber seemed more important than lignin in determining digestibility values. The significance of the term L/ADF in relation to digestibility estimates among all data and among genera was much less for in vitro estimates than for estimates calculated from predictive equations. This may indicate that lignification of cellulose has less influence on digestibility of tropical than of temperate forages. The estimated digestibility values based on fibrous constituents were poorly correlated to in vitro digestibility values, indicating inadequacy of using predictive equations developed for temperate forages with tropical grasses. Correlation coefficients between measured parameters showed great variation among four genera: Paspalum, Pennisetum, Digitaria, and Panicum.

scholarly journals COMPARISON OF NEAR INFRARED CALIBRATIONS FOR ESTIMATING IN VITRO DIGESTIBILITY IN WHOLE-PLANT CORN HYBRIDS

1987 ◽  
Vol 67 (2) ◽  
pp. 557-562 ◽  
Author(s):  
E. V. VALDES ◽  
R. B. HUNTER ◽  
G. E. JONES
Keyword(s):  
Dry Matter ◽  
Near Infrared ◽  
Mature Stage ◽  
In Vitro Digestibility ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
Corn Hybrids ◽  
Dry Matter Digestibility ◽  
Whole Plant

A comparison of two near infrared (NIRA) calibrations (C1 and C2) for the prediction of in vitro dry matter digestibility (IVDM) in whole-plant corn (WPC) was conducted. C1 consisted of 40 WPC samples collected from four locations across Ontario (Brucefield, London, Guelph and Elora). C2 consisted of 90 samples and included the above locations plus Pakenham and Winchester. Nine wavelengths were used in both equations but only three were common in C1 and C2 equations. These wavelengths were 2139 nm, 2100 nm, and 1445 nm, respectively. The predictions of IVDM utilizing both C1 and C2 were good. Coefficients of determination (r2) and standard error of the estimate (SEE) for calibration and prediction sets were 0.91, 1.7; 0.85, 1.7 for C1 and 0.88, 1.6; 0.77, 1.6 for C2 respectively. Regression analysis within location, however, showed low r2 values for the prediction of IVDM for Pakenham and Winchester in both calibrations. The more mature stage of harvest at these locations might be the cause of the poorer predictions. Key words: In vitro digestibility, whole-plant corn, near infrared reflectance

THE IN VITRO DIGESTIBILITY OF PLANT PARTS OF ORCHARDGRASS CLONES WITH ADVANCING STAGES OF MATURITY

1965 ◽  
Vol 45 (5) ◽  
pp. 503-507 ◽  
Author(s):  
D. N. Mowat ◽  
B. R. Christie ◽  
J. E. Winch
Keyword(s):  
Dry Matter ◽  
In Vitro Digestibility ◽  
Plant Parts ◽  
Dry Matter Digestibility ◽  
Total Leaf ◽  
Early Maturing

The in vitro dry matter digestibility (I.V.D.) of the stem and total leaf fractions of four orchardgrass clones was similar at the preheading stage. Even at 100% head emergence, differences in I.V.D. between stems and living leaves were not great with the early-maturing clones. However, wide differences existed between the values for stems and living leaves in mature plants. Heads were only slightly higher than stems in digestibility.Leaf digestibility was lower for those leaves dead or even partially dead. Nevertheless, in mature plants, dead leaves were higher in I.V.D. than heads or stems. Large differences occurred among clones in stem digestibility. However, the variability among clones in leaf digestibility was small.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

scholarly journals Yield and nutritional value of BRS Capiaçu grass at different regrowth ages

2019 ◽  
Vol 40 (5) ◽  
pp. 2045 ◽  
Author(s):  
Flávio Pinto Monção ◽  
Marco Aurélio Moraes Soares Costa ◽  
João Paulo Sampaio Rigueria ◽  
Marielly Maria Almeida Moura ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  
Keyword(s):  
Dry Matter ◽  
Northern Region ◽  
Matter Content ◽  
Neutral Detergent Fiber ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Elephant Grass ◽  
Dry Matter Digestibility

The objective was to evaluate the productivity, chemical composition, ruminal degradability of dry matter and digestibility of BRS capiaçu grass (Pennisetum purpureum Schum.) managed at five regrowth ages. A completely randomized design with ten replications was used in the BRS capiaçu elephant grass subjected to five cutting intervals (30, 60, 90, 120 and 150 days) in the summer, making a total of 50 plots with a useful area of 4 x 2 m. There were daily increases in the dry matter production in the order of 382 kg ha-1, with 49,859 kg ha-1 being produced at 150 regrowth days. The dry matter content and organic matter linearly increased (P < 0.01) at different regrowth ages. The crude protein content, in vitro dry matter digestibility and in vitro digestibility of neutral detergent fiber linearly reduced (P < 0.01) 0.037%, 0.196% and 0.256% per day, respectively. Potential degradability of dry matter decreased from 68.9% at 30 days to 44.7% at 150 regrowth days (0.194 percentage units per day). The rate of degradation of fraction B 'c' was not modified (P = 0.94), averaging 1.46% hour-1. In the cultivation of BRS capiaçu elephant grass in the summer season, in the northern region of Minas Gerais, the age for harvesting between 90 and 120 days of regrowth is recommended.

scholarly journals Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation

2005 ◽  
Vol 48 (spe) ◽  
pp. 161-168 ◽  
Author(s):  
Lúcia de Fátima Araújo ◽  
Ariosvaldo Nunes Medeiros ◽  
Américo Perazzo Neto ◽  
Líbia de Sousa Conrado Oliveira ◽  
Flávio Luiz Honorato da Silva
Keyword(s):  
Dry Matter ◽  
Solid Medium ◽  
Animal Feed ◽  
In Vitro Digestibility ◽  
Cactus Pear ◽  
Protein Supplements ◽  
Dry Matter Digestibility ◽  
Matter Production ◽  
Gross Energy

The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross energy value when compared to that of the cactus pear in natura.

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