Sugar-mediated semidian oscillation of gene expression in the cassava storage root regulates starch synthesis

The Policy Analysis Matrix (PAM) was used to assess the efficiencies and competitiveness of fresh cassava storage root production systems in Sierra Leone. Proportional random sampling was used to select study samples. Information was collected using structured questionnaire from a total of 1,880 producer households. Out of the 36 potential storage root production systems identified, only 6 systems are mainly used by producers. The PAM was based on one hectare of land for production and Leone (SSL) as money to evaluate costs and revenues. The analysis indicates that, all the 6 fresh cassava storage root production systems present a Domestic Resource Cost Ratio of less than 1 (DRC < 1) and Cost-Benefit Ratio (RCB) also less than 1 (RCB within 0.14 to 0.42). Discounting potential revenue from stems and cassava leaves in storage root production systems that use improved varieties and fertilizer have higher comparative and competitive advantages. The systems are also profitable, even though producers are not protected from tradable and taxed inputs. Production systems also remain profitable with 25% and 50% yield loss. This was also confirmed by Abiodun and Adefemi (2016). It is therefore better to produce cassava locally in Sierra Leone than import for processing or consumption. This study proposes recommendations to improve cassava productivity in Sierra Leone.

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Convolutional Neural Net-Based Cassava Storage Root Counting Using Real and Synthetic Images

Frontiers in Plant Science ◽

10.3389/fpls.2019.01516 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 3

Author(s):

John Atanbori ◽

Maria Elker Montoya-P ◽

Michael Gomez Selvaraj ◽

Andrew P. French ◽

Tony P. Pridmore

Keyword(s):

Neural Net ◽

Storage Root ◽

Synthetic Images ◽

Cassava Storage Root ◽

Root Counting

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Transcriptomic Analysis of Starch Biosynthesis in the Developing Grain of Hexaploid Wheat

International Journal of Plant Genomics ◽

10.1155/2009/407426 ◽

2009 ◽

Vol 2009 ◽

pp. 1-23 ◽

Cited By ~ 17

Author(s):

Boryana S. Stamova ◽

Debbie Laudencia-Chingcuanco ◽

Diane M. Beckles

Keyword(s):

Gene Expression ◽

Dna Microarrays ◽

Expression Profiles ◽

Expression Patterns ◽

Soluble Sugars ◽

Starch Synthesis ◽

Gene Families ◽

Starch Accumulation ◽

Protein Accumulation ◽

High Accumulation

The expression of genes involved in starch synthesis in wheat was analyzed together with the accumulation profiles of soluble sugars, starch, protein, and starch granule distribution in developing caryopses obtained from the same biological materials used for profiling of gene expression using DNA microarrays. Multiple expression patterns were detected for the different starch biosynthetic gene isoforms, suggesting their relative importance through caryopsis development. Members of the ADP-glucose pyrophosphorylase, starch synthase, starch branching enzyme, and sucrose synthase gene families showed different expression profiles; expression of some members of these gene families coincided with a period of high accumulation of starch while others did not. A biphasic pattern was observed in the rates of starch and protein accumulation which paralleled changes in global gene expression. Metabolic and regulatory genes that show a pattern of expression similar to starch accumulation and granule size distribution were identified, suggesting their coinvolvement in these biological processes.

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Starch synthesis and gelatinization properties of potato tubers

Ciência Rural ◽

10.1590/0103-8478cr20210050 ◽

2022 ◽

Vol 52 (4) ◽

Author(s):

Wang Su ◽

Guangji Ye ◽

Yun Zhou ◽

Jian Wang

Keyword(s):

Gene Expression ◽

Enzyme Activity ◽

Starch Granule ◽

Starch Content ◽

Starch Synthesis ◽

Starch Accumulation ◽

Enzyme Gene ◽

Middle Stage ◽

Pasting Temperature ◽

Starch Synthesis Enzyme

ABSTRACT: Biosynthesis is the only source of potato starch which is an important raw material for food processing, modified starch and biomass energy. However, it is not clear about the evolution of starch synthesis with tuber development in potato. The present study evaluated the differences of starch synthesis and gelatinization properties of potato tubers with different starch content. Relative to cultivars of medium and low starch content, cultivars of high starch content showed significantly higher SBEII gene expression, AGPase and SSS enzyme activity, and total starch content after middle stage of starch accumulation, and had smaller average starch granule size during whole process of tuber development, and had higher pasting temperature before late stages of tuber growth, and had lower pasting temperature after middle stage of starch accumulation. Path analysis showed that, after middle stage of starch accumulation, effects on starch gelatinization of cultivars with high, medium and low starch content represented starch synthesis enzyme activity > starch accumulation > starch granule distribution > starch synthesis enzyme gene expression, starch synthesis enzyme gene expression > starch synthesis enzyme activity > starch accumulation > starch granule distribution, starch synthesis enzyme gene expression > starch granule distribution > starch synthesis enzyme activity > starch accumulation, respectively. In the study, phases existed in the starch biosynthesis of potato tuber, and the starch quality and its formation process were different among varieties with different starch content. The findings might contribute to starch application and potato industries.

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Effects of RNA Interference of SBE3 Gene Expression on Starch Ac-cumulation and Key Enzymes Activities Involved in Starch Synthesis in Transgenic Rice Grain

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2010.00313 ◽

2010 ◽

Vol 36 (2) ◽

pp. 313-320 ◽

Cited By ~ 1

Author(s):

Jie-Ming WANG ◽

Jian ZHANG ◽

Hai-Yang JIANG ◽

Su-Wen ZHU ◽

Jun FAN ◽

...

Keyword(s):

Gene Expression ◽

Rna Interference ◽

Transgenic Rice ◽

Starch Synthesis ◽

Rice Grain ◽

Key Enzymes ◽

Enzymes Activities ◽

I Gene

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An optimized method to obtain high-quality RNA from cassava storage root

3 Biotech ◽

10.1007/s13205-019-1608-9 ◽

2019 ◽

Vol 9 (4) ◽

Author(s):

Lulu Guan ◽

Xiaowen Ma ◽

Xiaoxia Zhou ◽

Bowen Tan ◽

Zhen-Yu Wang

Keyword(s):

Storage Root ◽

High Quality ◽

Cassava Storage Root

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Studies toward the identification of transcription factors in cassava storage root

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202003000300006 ◽

2003 ◽

Vol 15 (3) ◽

pp. 167-170 ◽

Cited By ~ 2

Author(s):

Cláudia Regina Batista de Souza ◽

Elionor Rita Pereira de Almeida ◽

Luiz Joaquim Castelo Branco Carvalho ◽

Eugen Silvano Gander

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Manihot Esculenta ◽

Plant Traits ◽

Storage Root ◽

Brazil Nut ◽

Manihot Esculenta Crantz ◽

Physiological Processes ◽

Cassava Storage Root

Transcription factors play important roles in several physiological processes. In recent years many transcription factors have been isolated from plants and they are emerging as powerful tools in the manipulation of plant traits. In this work we initiated studies in order to isolate transcription factors from cassava (Manihot esculenta Crantz), an important tropical and subtropical crop. Our results show three kinds of proteins expressed differentially in cassava storage root and immunologically related to the opaque-2 transcription factor from maize. Southwestern experiments showed two proteins capable of interacting in vitro with the DNA sequence of the be2S1 gene from the Brazil nut tree.

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