Potential anticancer activity of Acetone extracts of Toona cilliata, Seriphium plumosum and Schkuhria pinnata on HeLa cervical cancer cells

Background: Cervical cancer is common in women in less developed regions of the world. The plant biomolecules can be employed for synergistic activity with chemo- and radiotherapy. This combinations might result in reduced toxicity and increased efficacy of the treatment regimen. Objectives: The anti-HeLa cells activity of the acetone extracts of S. plumosum, T. cilliata and S. pinnata was assessed using different parameters. Methods: Secondary metabolite detection and antioxidant activity quantification were determined using the DPPH and ferric iron reducing assays. HeLa cell growth inhibition and mechanistics were assessed by employing MTT and Annexin-V flous assays. Results: Observations revealed the presence of phenolic, flavonoids, tannins steroids and coumarins in all the plants ex- tracts. High amount of total phenolic and flavonoid content were detected in S. plumosum and T. cilliata. S. plumosum extract had the best DPPH scavenging activity and ferric reducing powers. Conclusion: Observable concentration dependent cell proliferation inhibition by test materials was exhibited. The leaf ex- tracts from T. cilliata, S. plumosum and S. pinnata contain compounds of various polarities with free-radical, antioxidant and anti-cancerous activities that may play a beneficial role in treatment. Keywords: Medicinal plants; anticancer activity; antioxidant activity.

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Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i830406 ◽

2021 ◽

pp. 1-9

Author(s):

Gerard Bessan Dossou-Agoin ◽

Habib Ganfon ◽

Fidèle Assogba ◽

Adam Gbankoto ◽

Joachim Gbenou ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Reducing Power ◽

Total Phenolic ◽

Scavenging Activity ◽

Aqueous Extracts ◽

Fruit Extract ◽

Stem Extract ◽

Dpph Scavenging

Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract. Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.

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Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47111325 ◽

2018 ◽

Vol 47 (2) ◽

pp. 384-389

Author(s):

Sebnem Selen ISBILIR ◽

Sevilay Inal KABALA ◽

Hulya YAGAR

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Antioxidant Activities ◽

Inhibitory Effect ◽

Total Phenolic ◽

Flower Bud ◽

Ethanol Extracts ◽

Dpph Scavenging ◽

Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

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Sunflower (Helianthus annuus L.) Plants at Various Growth Stages Subjected to Extraction—Comparison of the Antioxidant Activity and Phenolic Profile

Antioxidants ◽

10.3390/antiox9060535 ◽

2020 ◽

Vol 9 (6) ◽

pp. 535 ◽

Cited By ~ 1

Author(s):

Francesco Gai ◽

Magdalena Karamać ◽

Michał A. Janiak ◽

Ryszard Amarowicz ◽

Pier Giorgio Peiretti

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Total Phenolic ◽

Growth Stages ◽

Scavenging Activity ◽

Flowering Stage ◽

Phenolic Profile ◽

Aerial Parts ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

The aim of this study was to evaluate the differences in the antioxidant activity and phenolic profile of sunflower (Helianthus annuus L.) extracts obtained from the aerial parts of plants harvested at five growth stages. In vitro assays were used to determine the antioxidant activity, i.e., ABTS•+ and DPPH• scavenging activity, the ferric-reducing antioxidant power (FRAP) and the ability to inhibit β-carotene–linoleic acid emulsion oxidation. Phenolic compounds, such as mono- and dicaffeoylquinic acid isomers and caffeic acid hexose, were identified using the LC–TOF–MS/MS technique. The predominant compound during the growth cycle of the plant was 3,5-di-O-caffeoylquinic acid, whose content was the highest at the mid-flowering stage. The total phenolic content was also the highest in sunflowers at the mid-flowering stage. The main phenolic compound contents were closely correlated with ABTS•+ and DPPH• scavenging activity and FRAP. No significant correlation was found between the total phenolic content and the antioxidant activity in the emulsion system. The highest antiradical activity and FRAP were generally determined in older plants (mid-flowering and late flowering stages). In conclusion, the aerial parts of sunflowers, in particular those harvested at the mid-flowering stage, are a good plant material from which to obtain phenolic compound extracts, albeit mainly of one class (esters of caffeic acid and quinic acid), with high antioxidant activity.

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Anticancer Potential of Fruit Extracts from Vatica diospyroides Symington Type SS and Their Effect on Program Cell Death of Cervical Cancer Cell Lines

The Scientific World JOURNAL ◽

10.1155/2019/5491904 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Atchara Chothiphirat ◽

Kesara Nittayaboon ◽

Kanyanatt Kanokwiroon ◽

Theera Srisawat ◽

Raphatphorn Navakanitworakul

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Cell Lines ◽

Morphological Changes ◽

Annexin V ◽

Siha Cell ◽

Caspase 8 ◽

Dependent Manner ◽

Cervical Cancer Cells ◽

Dose Dependent

Vatica diospyroides Symington is locally known as Chan-Ka-Pho in Thailand. Ancient people have used it as therapeutic plant for cardiac and blood tonic cure. The purpose of this study was to investigate the potential cytotoxicity and selectivity of the extracts from V. diospyroides type SS fruit on cervical cancer HeLa and SiHa cell lines and to examine its underlying mechanism of action. MTT assay revealed that the extracts showed inhibition of cell survival in a dose-dependent manner and exhibited highly cytotoxic activity against both HeLa and SiHa cells with IC50 value less than 20 μg/mL along with less toxicity against L929 cells. Acetone cotyledon extract (ACE) showed the best selectivity index value of 4.47 (HeLa) and 3.51 (SiHa). Distinctive morphological changes were observed in ACE-treated cervical cancer cells contributing to apoptosis action. Flow cytometry analysis with Annexin V-FITC and PI staining precisely indicated that ACE induced apoptosis in HeLa and SiHa cell lines in a dose-dependent manner. Treatment of ACE with half IC50 caused DNA fragmentation and also activated increasing of bax and cleaved caspase-8 protein in HeLa cells after 48 h exposure. The results suggest that ACE has potent and selective cytotoxic effect against cervical cancer cells and the potential to induce bax and caspase-8-dependent apoptosis. Hence, the ACE could be further exploited as a potential lead in cancer treatment.

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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells

Journal of Toxicology ◽

10.1155/2020/8871152 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Michèle Stella Majoumouo ◽

Marius Belmondo Tincho ◽

Rufin Marie Kouipou Toghueo ◽

Thureyah Morris ◽

Donavon Charles Hiss ◽

...

Keyword(s):

Cervical Cancer ◽

Endophytic Fungi ◽

Hela Cells ◽

Annexin V ◽

Bioactive Metabolites ◽

Terminalia Catappa ◽

Trichoderma Sp ◽

Cervical Cancer Cells ◽

Late Apoptosis ◽

Human Cervical Cancer

Endophytic fungi are potential sources of novel bioactive metabolites from a natural product drug discovery perspective. This study reports the bioactivity-directed fractionation of the secondary metabolites of the ethyl acetate extract of a fermentation culture of endophytic fungi from Terminalia catappa which were then evaluated for their cytotoxicity against human cervical cancer (HeLa) cells and human foreskin fibroblast (HFF) cells. Furthermore, apoptosis was determined using the Annexin V/propidium iodide (PI) flow cytometry assay. Endophyte extracts N2, N7, N8, N97, N169, and N233 were obtained from Trichoderma sp, Phoma sp, Phomopsis phyllanticola, Fusarium oxyporum, Collectotrichum sp, and Cryptococcus flavescens, respectively. The N97 extract was most active with a 50% inhibitory concentration (IC50) of 33.35 µg/ml. A 50% cytotoxic concentration (CC50) of 268.4 µg/ml was obtained with HFF cells and the selectivity index (SI) was 8.01. The percentages of cell populations were increased at late apoptosis (Annexin+/PI+), with the percentages of 27.4 ± 0.3 and 19.2 ± 0.01 obtained, respectively, for 50 µg/ml and 80 µg/ml of the N97 extract and 2.1 ± 0.1 obtained for the control in late apoptosis (Annexin V+/PI+) . Moreover, a higher reduction in the percentage of viable cells was observed in the HeLa control cells (93.6 ± 0.3), but the percentages of viable HeLa cells were 37 ± 0.05 and 45 ± 0.1, respectively, for the 50 µg/ml and 80 µg/ml treatments with the N97 extract. Also, the percentages of 34.7 ± 0.1 and 33.9 ± 0.4 were, respectively, obtained for 50 µg/ml and 80 µg/ml compared to the control with 4.6 ± 0.2, in early apoptosis (Annexin V+/PI-). These findings highlight the anticancer potential of the N97 extract of endophytic fungi from Terminalia catappa, which is mediated through apoptosis and presumably also attenuation of chemoresistance.

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Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

Biomolecules ◽

10.3390/biom9090494 ◽

2019 ◽

Vol 9 (9) ◽

pp. 494 ◽

Cited By ~ 2

Author(s):

Khumkhrong ◽

Piboonprai ◽

Chaichompoo ◽

Pimtong ◽

Khongkow ◽

...

Keyword(s):

Cervical Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Mechanism Of Action ◽

Epithelial Mesenchymal Transition ◽

Cancer Chemoprevention ◽

Bioactive Compound ◽

Perennial Herb ◽

Mesenchymal Transition ◽

Cervical Cancer Cells

Crinum asiaticum is a perennial herb widely distributed in many warmer regions, including Thailand, and is well-known for its medicinal and ornamental values. Crinum alkaloids contain numerous compounds, such as crinamine. Even though its mechanism of action is still unknown, crinamine was previously shown to possess anticancer activity. In this study, we demonstrate that crinamine was more cytotoxic to cervical cancer cells than normal cells. It also inhibited anchorage-independent tumor spheroid growth more effectively than existing chemotherapeutic drugs carboplatin and 5-fluorouracil or the CDK9 inhibitor FIT-039. Additionally, unlike cisplatin, crinamine induced apoptosis without promoting DNA double-strand breaks. It suppressed cervical cancer cell migration by inhibiting the expression of positive regulators of epithelial-mesenchymal transition SNAI1 and VIM. Importantly, crinamine also exerted anti-angiogenic activities by inhibiting secretion of VEGF-A protein in cervical cancer cells and blood vessel development in zebrafish embryos. Gene expression analysis revealed that its mechanism of action might be attributed, in part, to downregulation of cancer-related genes, such as AKT1, BCL2L1, CCND1, CDK4, PLK1, and RHOA. Our findings provide a first insight into crinamine's anticancer activity, highlighting its potential use as an alternative bioactive compound for cervical cancer chemoprevention and therapy.

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Antioxidant Properties of Crude Extract, Partition Extract, and Fermented Medium of Dendrobium sabin Flower

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/2907219 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Farahziela Abu ◽

Che Norma Mat Taib ◽

Mohamad Aris Mohd Moklas ◽

Sobri Mohd Akhir

Keyword(s):

Antioxidant Activity ◽

Crude Extract ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Properties ◽

Total Phenolic ◽

Correlation Relationship ◽

Good Potential ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Antioxidant properties of crude extract, partition extract, and fermented medium from Dendrobium sabin (DS) flower were investigated. The oven-dried DS flower was extracted using 100% methanol (w/v), 100% ethanol (w/v), and 100% water (w/v). The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract) and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on DS flower medium showed a potential in increasing the phenolic content and DPPH scavenging activity. The TPC of final fermented medium showed approximately 18% increment, while the DPPH of fermented medium increased significantly to approximately 80% at the end of the fermentation. Dendrobium sabin (DS) flower showed very good potential properties of antioxidant in crude extract and partition extract as well as better antioxidant activity in the flower fermented medium.

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Anticancer activity of arborinine from Glycosmis parva leaf extract in human cervical cancer cells

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2018.04.175 ◽

2018 ◽

Vol 500 (4) ◽

pp. 866-872 ◽

Cited By ~ 7

Author(s):

Kitiya Piboonprai ◽

Phattharachanok Khumkhrong ◽

Mattaka Khongkow ◽

Teerapong Yata ◽

Nijsiri Ruangrungsi ◽

...

Keyword(s):

Cervical Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Leaf Extract ◽

Cervical Cancer Cells ◽

Human Cervical Cancer Cells ◽

Human Cervical Cancer

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Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

Functional Plant Biology ◽

10.1071/fp11016 ◽

2011 ◽

Vol 38 (9) ◽

pp. 697 ◽

Cited By ~ 17

Author(s):

Arvind K. Goyal ◽

Bharat C. Basistha ◽

Arnab Sen ◽

Sushil K. Middha

Keyword(s):

Antioxidant Activity ◽

Sea Buckthorn ◽

Total Phenolic ◽

Scavenging Activity ◽

Methanol Extracts ◽

Hippophae Salicifolia ◽

Total Antioxidant ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

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