scholarly journals Antimicrobial activity of Sida acuta, Phyllanthus amarus and Phyllanthus muellerianus against microorganisms implicated in urinary tract infections

2021 ◽  
Vol 23 (2) ◽  
pp. 153-168
Author(s):  
T. M. Obuotor ◽  
A. O. Kolawole ◽  
F. O. Adeyanju ◽  
S. S. Adewumi
Keyword(s):  
Antimicrobial Activity ◽  
Urinary Tract ◽  
Medicinal Plants ◽  
Crude Extract ◽  
Phytochemical Analysis ◽  
Phyllanthus Amarus ◽  
Leaf Extracts ◽  
Sida Acuta ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Increasing level of antimicrobial resistance among bacterial pathogens causing Urinary Tract Infection (UTI) is one of the most significant public health challenges globally. Hence, the search for alternatives from medicinal plants. This study investigated the efficacy of Phyllanthus amarus (PA), Phyllanthus muellerianus (PM) and Sida acuta (SA) leaf extracts on microorganisms implicated in UTI. Mid-stream urine samples collected from 100 patients clinically diagnosed with UTI were cultured. The microorganisms isolated were identified using their morphological and biochemical characteristics. Methanol leaf extracts of the three plants were obtained by cold maceration in 60% methanol. Crude extract of PM was thereafter purified by solvent partitioning. Antibiotic susceptibility test was determined using the Kirby Bauer disc diffusion. Antimicrobial effects of the extracts and oil was ascertained using agar well diffusion. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentrations (MBC) were also determined. Rate of kill and mechanism of action of the purified extract of PM on isolates were investigated. Cytotoxicity of plant extracts were assayed on brine shrimps while synergism of the purified extract with ciprofloxacin was ascertained using overlay inoculum susceptibility disc method. Antioxidant and phytochemical analyses of the extracts were conducted using standard methods. Phytochemical analysis of the leaf extracts showed the presence of alkaloids, tannins, saponins and steroids. Antioxidant assay also indicated SA had the highest total flavonoids and phenol content of 339.86 mgQUE/g and 27.63 mgGAE/g. Microorganisms isolated include: Escherichia coli (24%), Proteus mirabilis (24%), Staphylococcus aureus (19%), Klebsiella pneumoniae (13%), Candida albicans (11%), Enterobacter sp. (5%) and Citrobacter sp. (4%). The crude extract of PA had zone of inhibition ranging from 16.7 ± 1.53 mm to 24 ± 1.00 mm while SA crude extract had 14.7 ± 1.53 mm to 27 ± 2.00 mm. PM crude extract had inhibition zones of 17 ± 1.00 mm to 22.3 ± 2.12 mm. The MIC and MBC ranged from 6.25 mg/ml to 50 mg/ml and 12.5 mg/ml to 50 mg/ml respectively. Ethyl acetate fraction of PM showed the highest percentage yield and had a zone diameter range from 13.5 ± 1.00 mm to 28 ± 1.53 mm with MIC and MBC ranges of 6.25 mg/ml – 12.5 mg/ml and 25 mg/ml to 50 mg/ml respectively. Synergism with ciprofloxacin was observed at 25% of the microorganisms, 50% antagonism and 25% additively. Toxicity analysis showed lethal dose concentrations of 19.05 mg/ml, 25.12 mg/ml and 130.11 mg/ml for PM, PA and SA respectively. The findings of this study suggest that the methanol extracts of the medicinal plants used in this study does possess a potent lead molecule in combating microorganisms causing UTI. Key words: Antimicrobial activity, Phyllanthus muellerianus, Phytochemicals, Toxicity, UTI,

scholarly journals Antimicrobial activity of medicinal plants used for urinary tract infections in pastoralist community in Ethiopia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Eshetu Gadisa ◽  
Elazar Tadesse
Keyword(s):  
Antimicrobial Activity ◽  
Urinary Tract ◽  
Medicinal Plants ◽  
Urinary Tract Infections ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Resistant Bacteria ◽  
Chemical Structures ◽  
Tract Infections

Abstract Background Medicinal plants have wide medicament application used to prevent and management of many ailments. These plants are used for primary health care in pastoralist communities who are deprived of modern medical care. They possess extensive therapeutics bioactive coupled with varied chemical structures. However, scientific validation of efficacy and safety of plants used to treat the urinary tract infections haven’t been fully exploited. The aim of this study was to evaluate antimicrobial activity and screening phytochemicals of medicinal plants used to treat urinary tract infections. Methods In-vitro experimental study was carried out to evaluate the antimicrobial effect and screening phytochemical of Rumex abyssinicus, Cucumis pustulatus, Discopodium penninervium, Lippia adoensis, Euphorbia depauperata, and Cirsium englerianum. Against drug resistance microbes. 80% methanol was used for extraction of the plant parts. The susceptibility tests were investigated using disc diffusion and broth micro-dilution methods. Results The majority of tested extracts showed antimicrobial activity on two or more drug-resistant bacteria with MIC value (1.0–128.0 μg/ml) and 9–27 mm inhibition zone in diameter. Extracts obtained from C.englerianum and E. depauperate showed more potent antibacterial activity on MRSA and Enterococcus faecalis with IZ 25 and 27 mm respectively. E. coli and K. pneumoniae were inhibited by those extracts with IZ ranging 9–25 mm and 11–27 mm respectively. E.faecalis and K. pneumoniae were more susceptible bacteria to the respective extracts. R. abyssinicus showed promising antifungal effect with had 21 mm IZ and MIC range 16-32 μg/ml on C.albicans. Alkaloids, flavonoids, phenolic and terpenoid were common phytochemical characterized in majority of screened plants. Conclusion Tested extracts exhibited significant antibacterial and antifungal activity. Hence, further structural elucidation of bioactive that inhibited the growth of microbes aforementioned plants may be used as precursors for the synthesis of new antibiotics in the future.

Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

2020 ◽  
Vol 2020 ◽  
Author(s):  
Firew Admasu
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Traditional Medicine ◽  
Crude Extract ◽  
Susceptibility Test ◽  
Plant Sample ◽  
Traditional Medicinal Plants ◽  
Traditional Health Care ◽  
Susceptibility Tests

Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

scholarly journals COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITY OF TULSI (OCIMUM SANCTUM) AND NEEM (AZADIRACHTA INDICA) METHANOL EXTRACT

2018 ◽  
Vol 11 (12) ◽  
pp. 514
Author(s):  
Vipul Kumar ◽  
Anurag Chakraborty ◽  
Manpreet Kaur ◽  
Sony Pandey ◽  
Manoj Kumar Jena
Keyword(s):  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Extraction Process ◽  
Ocimum Sanctum ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Neem Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Escherichia Coli Bacteria

Objective: This study was focused on to compare the antimicrobial activity of methanolic leaf extracts of tulsi and neem.Methods: We have chosen tulsi (Ocimum sanctum) and neem (Azadirachta indica) to compare their antimicrobial activity toward Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Phytochemical extracts have been made by the use of methanol as solvent and dried leaf powder by the cold maceration extraction process. Phytochemical analysis for some secondary metabolites has been done using standard protocols. Nutrient agar plates were inoculated with the above-mentioned microorganisms by spreading bacterial inoculum on the surface of the media. Wells (6 mm in diameter) were punched in the agar. The phytochemical extracts of neem and tulsi were allowed to diffuse into the medium, and after incubation of 24 h at 37°C, the zones of inhibition were observed.Results: Statistical analysis showed that tulsi was more effective toward S. aureus while neem was more effective toward E. coli. Minimum inhibitory concentration (MIC) of tulsi for both the microorganisms was 0.4 g/ml, and the MIC of neem for both the microorganisms was 0.2 g/ml. When both the tulsi and neem extract were mixed with each other for every concentration at equal volume, they have shown better effects in comparison to individual neem or tulsi extract and also the MIC got reduced to 0.2 g/ml for both the bacteria.Conclusions: Leaf extracts of both the A. indica and O. sanctum have shown antimicrobial activity against E. coli and S. aureus. A. indica has higher antimicrobial activity against S. aureus, whereas the O. sanctum was found to be more effective against E. coli (indicated by the zone of inhibition). When both the extracts were mixed with each other, they have shown a better effect toward both bacteria.

scholarly journals BIO-CONTROL OF MULTIPLE DRUG-RESISTANT UROPATHOGENS USING MEDICINAL PLANT EXTRACTS

2019 ◽  
pp. 371-376
Author(s):  
VIGI CHAUDHARY ◽  
RAGHUVANSHI RK ◽  
NAVEEN CHAUDHARY ◽  
GAURAV SHARMA
Keyword(s):  
Urinary Tract ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Human Pathogens ◽  
Multiple Drug Resistant ◽  
Medicinal Plant Extracts ◽  
Tract Infections

Objective: The present study was conducted to evaluate the potential of some medicinal plants used in Ayurveda in treating multiple drug-resistant human pathogens causing urinary tract infections (UTIs). Methods: Dried parts of six medicinal plants used in Ayurveda for treating UTI were Soxhlet extracted, and the extract was concentrated in vacuo. Various concentrations of the extract were tested for antimicrobial activity against three clinical isolates of multiple drug-resistant bacteria causing UTI. Results: Preliminary results showed the promising antibacterial effect of plant extracts. Escherichia coli, the most common pathogen associated with UTI, was susceptible to aqueous extracts of all the six medicinal plants. Conclusion: This study concluded that the medicinal plants used in Ayurveda to treat UTIs are effective against multiple drug-resistant uropathogens. Further study in this regard may lead to the identification of novel antimicrobial agent for treating multiple drug-resistant urinary tract pathogens.

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