scholarly journals Fractionation of carbohydrates and proteins and in vitro rumen kinetics of corn silage under various storage durations

2021 ◽  
Vol 51 (4) ◽  
pp. 523-532
Author(s):  
S. Naetzold ◽  
J. Viégas ◽  
F.R. Skonieski ◽  
T.J. Tonin ◽  
L.L. Schumacher ◽  
...  
Keyword(s):  
Nutritional Value ◽  
Protein Quality ◽  
Corn Silage ◽  
Soluble Carbohydrate ◽  
Prolonged Storage ◽  
Ruminal Degradability ◽  
Corn Plants ◽  
True Protein ◽  
Kinetics Of

The experiment evaluated the effects on the chemical composition (fermentation, kinetic parameters, and protein and carbohydrate fractions) of corn silage after various storage durations (45, 90, 180, and 360 days). Experimental mini silos of the corn plants were made and opened after various storage periods. The experimental design was completely randomized with four treatments and four replications per treatment. Variables such as pH, lactic acid and acetic acid showed a quadratic effect, whereas effluent and gas losses increased linearly with prolonged storage. Proteolysis was observed with an increase in storage, resulting in a rise in ammonia-N, soluble N, and non-protein N concentration and reduction in true protein. The soluble carbohydrate fractions were reduced, and digestible and indigestible fibre were increased. For the kinetic degradation parameters of the silages obtained through in vitro gas methodology, the highest fermentation rate occurred in the first 12 hours of incubation, and the highest volume of gas produced within 96 hours was obtained for silages with shorter storage (45 days). The increase in storage resulted in greater losses and lower ruminal degradability in vitro, causing a decrease in protein quality and nutritional value.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

scholarly journals Kinetics of In Vitro Gas Production and Fitting Mathematical Models of Corn Silage

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 298
Author(s):  
Camila da Silva Zornitta ◽  
Luis Carlos Vinhas Ítavo ◽  
Camila Celeste Brandão Ferreira Ítavo ◽  
Geraldo Tadeu dos Santos ◽  
Alexandre Menezes Dias ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Gas Production ◽  
Feed Additives ◽  
Corn Silage ◽  
Feed Additive ◽  
Coefficient Of Determination ◽  
Production Data ◽  
In Vitro Gas Production ◽  
Kinetics Of

This study aimed at examining the effects of rumen inoculum of steers receiving different combinations of ionophore and probiotics in their diets on in vitro gas production of corn silage. The fitting of gas production was performed with five mathematical models and its kinetics was evaluated. Four crossbred steers (403.0 ± 75.5 kg body weight) with ruminal cannula were assigned to a 4 × 4 Latin square design. The additives used were Monensin sodium (Rumensin® 100, 3 g/day), Bacillus toyonensis (Micro-Cell Platinum® 109, 1 g/day) and Saccharomyces cerevisiae boulardii (ProTernative®20, 0.5 g/day). Additives were arranged into the following treatments, supplied daily into total mixed diet: (1) Monensin; (2) Monensin + B. toyonensis; (3) Monensin + S. boulardii; and (4) B. toyonensis + S. boulardii. The gas production data were fitted into the models of Gompertz, Groot, Ørskov, Brody, Richards, and Dual-pool Logistic. A perfect agreement between observed and predicted values in curves of accumulated in vitro gas production was observed in the Groot and Richards models, with higher coefficient of determination (R2 = 0.770 and 0.771, respectively), concordance correlation coefficient (CCC = 0.871 and 0.870, respectively), and root mean square error of prediction (RMSEP = 1.14 and 1.15, respectively). Evaluating the feed additives throughout the Groot model, the B. toyonensis + S. boulardii treatment presented higher VF (12.08 mL/100 mg of DM; p = 0.0022) than Monensin and Monensin + S. boulardii (9.16 and 9.22 mL/100 mg of DM, respectively). In addition, the fractional rate of gas production (k) was higher (p = 0.0193) in B. toyonensis + S. boulardii than in Monensin, not presenting a statistical difference (p > 0.05) from the other two treatments. Additionally, with the time of beginning to gas production, the lag time (λ), was greater (p < 0.001) with Monensin and Monensin + B. toyonensis than with Monensin + S. boulardii and B. toyonensis + S. boulardii. The combination of Monensin and probiotics (B. toyonensis + S. boulardii) resulted in better kinetics of degradation of corn silage, being that the Groot and Richards models had the best fit for estimates of the in vitro gas production data of corn silage tested with different feed additive combinations.

Revision: Indicadores del deterioro de la calidad proteica y del valor nutritivo de la leche / Review: Indicators of damage of protein quality and nutritional value of milk

1999 ◽  
Vol 5 (6) ◽  
pp. 447-461 ◽  
Author(s):  
E. Ferrer ◽  
A. Alegría ◽  
R. Farré ◽  
P. Abellán ◽  
F. Romero
Keyword(s):  
Maillard Reaction ◽  
Nutritional Value ◽  
Protein Quality ◽  
Storage Conditions ◽  
In Vitro Digestibility ◽  
Thermal Treatments ◽  
Maillard Reactions ◽  
Reducing Substances ◽  
Subsequent Storage

The nutritional value of milk can be negatively affected by thermal treatments. The quality of the proteins can be modified as a consequence of a set of reactions, the most important of which are the interactions between protein and carbohydrates, also known as Maillard reactions. These reactions can also occur when the storage conditions of moisture and temperature are unfavorable. The differ ent thermal treatments involved in processing milk (pasteurization, sterilization, drying, etc.) lead to different steps of the Maillard reaction and therefore to the formation of different compounds that can be useful as chemical markers of milk alteration, or more exactly of protein alteration. Estimating these markers is useful in monitoring the changes, especially those related to proteins, that happen during processing and storage. A review of the literature on the markers used to evaluate the modi fications that milk undergoes during thermal treatment and subsequent storage was carried out, focusing mainly on proteins. Special attention was paid to the analytical aspects. The chemical mark ers taken into account can be classified into two groups according to their relation with the Maillard reaction: 1. Specific markers of Maillard reaction: undesirable compounds (furosine, lisinoalanine, histidinalanine, furfurals, melanoidins); loss of nutrients (available lysine). 2. Non-markers of Maillard reaction: galactose; lactulose; protein-reducing substances; denaturation of protein; in vitro digestibility of proteins; others—pH, viscosity, free fatty acids.

scholarly journals Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate

2015 ◽  
Vol 14 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Abdelfattah Z M Salem ◽  
German Buendía-Rodríguez ◽  
Mona M M Elghandour ◽  
María A Mariezcurrena Berasain ◽  
Francisco J Peña Jiménez ◽  
...  
Keyword(s):  
Gas Production ◽  
Corn Silage ◽  
Production Kinetics ◽  
Salix Babylonica ◽  
Kinetics Of

Evaluation of protein quality of poultry feedingstuffs. 3. Expeller-processed oilseed cakes

1980 ◽  
Vol 94 (1) ◽  
pp. 117-123
Author(s):  
S. S. Zombade ◽  
G. N. Lodhi ◽  
J. S. Ichhponani
Keyword(s):  
Nutritive Value ◽  
Protein Quality ◽  
Nitrogen Solubility ◽  
Protein Digestibility ◽  
Regression Equations ◽  
Crude Fibre ◽  
Accuracy And Precision ◽  
True Protein

SummaryA study was made to evaluate the protein quality of groundnut cake (GN), mustard cake (MS) and cottonseed cake (CS) and to find a suitable laboratory test for predicting the protein quality of these cakes for chicks. Different oilseed cakes varied considerably in their crude protein, true protein and crude fibre content. The nitrogen solubility was higher in GN (87·7%) followed by MS (78·7%) and CS (60·9%). A similar trend was evident with regard to in vitro protein digestibility. The GN protein was, however, lower in available lysine (2·54%) than MS (3·97%) or CS (3·19%). The albumin, globulin, prolamin and glutelin fractions accounted for 74·1, 57·0 and 48·0% in GN, MS and CS, respectively. The nitrogen solubility, globulin content and modified relative nutritive value (RNV) correlated (P < 0·05) with gross protein value (GPV). The accuracy and precision of various regression equations for predicting the quality of protein in GN, MS and CS are discussed.

scholarly journals Anti-nutritional potential of protodioscin and kinetics of degradation in Urochloa grasses

2016 ◽  
Vol 37 (4) ◽  
pp. 2247 ◽  
Author(s):  
Eduardo Souza Leal ◽  
Luís Carlos Vinhas Ítavo ◽  
Cacilda Borges do Valle ◽  
Camila Celeste Brandão Ferreira Ítavo ◽  
Alexandre Menezes Dias ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Leaves Of Grass ◽  
Exotic Grasses ◽  
Kinetics Of Degradation ◽  
Kinetics Of

This study aimed to evaluate the nutritional value of Urochloa exotic grasses to quantify aspects of anti-quality and to identify their correlation with quality grasses. We evaluated the grasses U. humidicola cv. Comum, U. humidicola cv. BRS Tupi, U. decumbens cv. Basilisk, D70 U. decumbens and U. ruziziensis ecotype R124, and we grouped them into summer, autumn, winter and spring. We determined the chemical composition, in vitro digestibility, levels of protodioscin and cumulative gas production from the leaves of grass materials in nature. Basilisk and D70 showed higher content protodioscin in all seasons, with the highest values (31.4 and 27.4 g kg-1, respectively) in spring. D70 had a crude protein content of 140.0 g kg-1 in summer and a better in vitro digestibility of dry matter (888.7 g kg-1). R124 had a higher cumulative gas production in the spring (16.44 mL gas 100 mg DM-1). U. humidicola (Comum and BRS Tupi) presented lower protodioscin concentrations (1.22 and 1.07 g kg DM-1, respectively), and U. decumbens (Basilisk and D70) presented higher concentrations (27.25 and 24.55 g kg DM-1, respectively). The presence of protodioscin interfered with in vitro digestibility results and cumulative gas production in vitro.

scholarly journals In vitro Determination of the Release Kinetics of Peptides and Free Amino Acids During the Digestion of Food Proteins

2005 ◽  
Vol 88 (3) ◽  
pp. 935-948 ◽  
Author(s):  
Laurent Savoie ◽  
Rafael A Agudelo ◽  
Sylvie F Gauthier ◽  
Johanne Marin ◽  
Yves Pouliot
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Protein Quality ◽  
Release Kinetics ◽  
In Vitro Digestion ◽  
Exchange Chromatography ◽  
Protein Sources ◽  
Kinetics Of ◽  
Peptide Fractions

Abstract The kinetics of peptide release during in vitro digestion of 4 protein sources (casein, cod protein, soy protein, and gluten) were investigated. Samples were sequentially hydrolyzed with pepsin (30 min) and pancreatin (2, 4, or 6 h) in a dialysis cell with continuous removal of digestion products. Nondialyzed digests were fractionated by ion-exchange chromatography and ultrafiltration. Animal proteins were digested at a greater rate than plant proteins. Target amino acids of specific enzymes appeared more rapidly in the dialyzed fractions when compared to other amino acids. Throughout the hydrolysis, nondialyzed digests contained a higher proportion of peptide mixtures with basic-neutral properties. Except for gluten, peptide fractions with molecular weights that exceeded 10 kDa (basic-neutral, BN &gt; 10) were rapidly hydrolyzed during the first 2 h of pancreatin digestion. The kinetics of release and the composition of peptide fractions were different when the protein sources were compared. The analysis of amino acids revealed that threonine and proline proportions were relatively high in BN &gt; 10 and in peptide fractions with molecular weight between 10-1 kDa (BN 10-1), while tyrosine, phenylalanine, lysine, and arginine predominated in the low molecular weight (&lt;1 kDa) fractions. More resistant peptides were generally rich in proline and glutamic acid. The role of in vitro digestion assays in dietary protein quality evaluation is discussed.

Effect of pH and inhibitors on beta-amyloid fibril assembly

1996 ◽  
Vol 54 ◽  
pp. 752-753
Author(s):  
Beverly E. Maleeff ◽  
Timothy K. Hart ◽  
Stephen J. Wood ◽  
Ronald Wetzel
Keyword(s):  
Amyloid Fibril ◽  
Peptide Fragment ◽  
Hydrophobic Peptides ◽  
Amyloid Fibril Formation ◽  
Effects Of Ph ◽  
Severity Of The Disease ◽  
Kinetics Of ◽  
The Brain

Alzheimer's disease is characterized post-mortem in part by abnormal extracellular neuritic plaques found in brain tissue. There appears to be a correlation between the severity of Alzheimer's dementia in vivo and the number of plaques found in particular areas of the brain. These plaques are known to be the deposition sites of fibrils of the protein β-amyloid. It is thought that if the assembly of these plaques could be inhibited, the severity of the disease would be decreased. The peptide fragment Aβ, a precursor of the p-amyloid protein, has a 40 amino acid sequence, and has been shown to be toxic to neuronal cells in culture after an aging process of several days. This toxicity corresponds to the kinetics of in vitro amyloid fibril formation. In this study, we report the biochemical and ultrastructural effects of pH and the inhibitory agent hexadecyl-N-methylpiperidinium (HMP) bromide, one of a class of ionic micellar detergents known to be capable of solubilizing hydrophobic peptides, on the in vitro assembly of the peptide fragment Aβ.

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