scholarly journals Evaluation of Wound Healing Potential of Chrysophyllum albidum Hexane Seed Extract in Wistar Rat

2019 ◽  
Vol 2 (1) ◽  
pp. 82-90
Author(s):  
R D Oladeji ◽  
O Babatunde ◽  
I A Ajayi
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Test Group ◽  
Antimicrobial Activities ◽  
Negative Control ◽  
Seed Extract ◽  
Wound Contraction ◽  
Contraction Rate ◽  
Wound Healing Activity ◽  
Antioxidant And Antimicrobial Activities

Phytochemical, antioxidant and antimicrobial activities of the seeds of Chrysophyllum albidum have been established. However, scientific evaluation of its wound healing activity is lacking. Thus, this study was aimed at evaluating the wound healing potential of Chrysophyllum albidum hexane seed extract using wistar rats. The seed cotyledon was collected, air-dried, blended and extracted with n-hexane by continuous cold extraction. Phytochemicals, antioxidant and antimicrobial activities of the extracts were assayed by standard methods. Three groups of male wistar rats consisting of seven animals each were used for the animal studies. Wound excision was induced on all animals in the sterilized shaved dorsal fur region.Group1 served as the negative control (untreated), groups 2 and 3 were treated with n-hexane extract (test group) and Povidone-iodine ointment (positive control) respectively. Wound contraction rate and epithelialization time were used to assess the efficiency of the treatment. Group 3 rats had best wound contraction rate with epithelialization time of 16 post-wounding day followed by group 2 (20 days) and group 1 (28 days). The wound healing efficiency of the extract was less than that of the standard ointment but it healed the wound inflicted on the rats faster than the untreated ones. The low wound activity of the seed extract may be due to low phytochemical, antioxidant and antimicrobial activity of the extract. Chrysophyllum albidum hexane seed extract have some wound healing activity and therefore have potential for being used as wound healing ointment.

scholarly journals A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats

2020 ◽  
Vol 13 (7) ◽  
pp. 1280-1287
Author(s):  
Kalbaza Ahmed Yassine ◽  
Hemida Houari ◽  
Benchohra Mokhtar ◽  
Amara Karim ◽  
Salem Hadjer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Histological Study ◽  
Test Group ◽  
Wound Contraction ◽  
Control Group ◽  
Petroleum Jelly ◽  
Lawsonia Inermis ◽  
Wound Healing Activity ◽  
Daily Application

Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats. Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed. Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p<0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p<0.05). Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent.

scholarly journals Experimental Evaluation of Ethanolic Extract ofCarapa guianensisL. Leaf for Its Wound Healing Activity Using Three Wound Models

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Joel Kanhai ◽  
David Malcolm Milne ◽  
Lexley Pinto Pereira ◽  
William H. Swanston
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Breaking Strength ◽  
Test Group ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Wound Healing Activity

The leaves ofCarapa guianensishave been used to treat ulcers, skin parasites, and skin problems. The ethanolic extract ofC. guianensisleaf was evaluated for its antibacterial and wound healing activity using excision, incision and dead space wound models in rats. The animals were randomly divided into two groups (n= 6) in all the models. In the excision wound model test group animals were treated topically with the leaf extract (250 mg kg−1body weight) whereas, control animals were treated with petroleum jelly. In the incision and dead space wound models, the test group animals were treated with extract (250 mg kg−1day−1) orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight and hydoxyproline content. On Day 15 extract-treated animals exhibited 100% reduction in the wound area when compared to controls (95%) with significant decrease in the epithelialization period. The extract failed to demonstrate antibacterial activity. Skin breaking strength (P< .001), wet (P< .002) and dry (P< .02) granulation tissue and hydroxyproline content (P< .03) were significantly higher in extract treated animals. The increased rate of wound contraction, skin breaking strength and hydroxyproline content supports potential application ofC. guianensisin wound healing.

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

scholarly journals Wound Healing Activity of Ethanolic Extract Gel of Tawas Ut Tuber (Ampelocissus rubiginosa L.) in Incisional Model Wistar Rats

2018 ◽  
Vol 23 (1) ◽  
pp. 30 ◽  
Author(s):  
Khoerul Anwar ◽  
Dewita Fitri Widodo ◽  
Nurlely Nurlely ◽  
Liling Triyasmono ◽  
Sudarsono Sudarsono ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Average Percentage ◽  
Wound Length ◽  
Wound Healing Activity

Tawas ut tuber (Ampelocissus rubiginosa Lauterb.) is empirically used in wound healing by Dayak community. The present study was undertaken to assess wound healing activity of gel from ethanol extract of A. rubiginosa tuber using parameters of the closure of wound length, tensile strength, and histopathological observations. This study used twenty-five male Wistar rats divided into five groups: positive control (Bioplacenton®), negative control (placebo gel), and three of testing groups with gel extract (concentrations of 1.5%, 2.0%, and 2.5%). Wound healing activity was evaluated to 4 cm length and ± 2 mm depth incision wound model on the back skin of rats. The measurement of wound length was observed on the day of 4th, 8th, 12th, 16th, and 20th used quantitative analyze. On the day of 20th, animal was anaesthetized and the skin has been taken for tensile strength evaluation and histopathological observations. This study showed that group treated with gel extract at the concentration of 2.5% experienced higher wound healing activity with average percentage of wound closure of 99.00% ± 0.16, tensile strength 3.8541 gram/mm2, and proved with the re-epithelization, neocapillarization, and increase collagen density appeared in histopathological observations. Based on this study, gel from ethanol extract of A. rubiginosa tuber possesses wound healing activity.

scholarly journals In-vivo Evaluation of the Wound Healing Activity of the Sesamum Indicum L. Seed Extract in Novel Ethosomal Vesicular System

2018 ◽  
Vol 8 (5) ◽  
pp. 411-420 ◽  
Author(s):  
Sachin B Somwanshi ◽  
Shivanand N Hiremath
Keyword(s):  
Wound Healing ◽  
Breaking Strength ◽  
Wistar Rat ◽  
Treated Group ◽  
Sesamum Indicum ◽  
Seed Extract ◽  
Wound Contraction ◽  
In Vivo Evaluation ◽  
Wound Healing Activity

Aim: To establish the wound healing activity of Sesamum indicum L. of seed extract in novel ethosomal vesicles. Methods: The ethosomal vesicles were formulated with prepared seed extract of Sesamum indicum L. by solvent evaporation method and characterized it. The optimized ethosomal vesicles then incorporated into gel base for further in vivo study in wistar rat. The evaluation of the wound healing activity was performed by using two models i.e. incision and excision models. In excision model percentage wound contraction and period of epithelialization were established for both the extracts. In incision model the parameter which was carried out was breaking strength of wounded skin. Results: The results revealed that the percentage wound contraction, period of epithelialization in excision model was enhanced than that of other groups and tensile strength of skin in incision model was similar to that of standard treated group. Keywords: Sesamum indicum L., Wound Healing, Ethosomal gel.

scholarly journals Evaluation of polyherbal ointment for wound healing activity in Wistar rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 26-31
Author(s):  
Shilpa S. Kolhe
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Breaking Strength ◽  
Skin Irritation ◽  
Wound Contraction ◽  
Alcoholic Extract ◽  
Polyherbal Formulation ◽  
Wound Healing Activity

The aim of present study to formulate, evaluate and to check the wound healing activity of newly prepared polyherbal formulation (ointment) .Herbal ointment containing hydrochloric extracts of plants Psorolea corylifalia, Achryanthes aspera ,was formulated as ointment and the hydro alcoholic extract was prepared by maceration method. These Formulations were evaluated for the following parameters: pH, Spread ability, grittiness, skin irritation study, stability. The wound healing activity is assessed by the rate of wound contraction, Period of epithelisation and skin breaking strength. Keywords: Herbal Ointment, Wound Healing Activity.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

scholarly journals Solanum incanum extract enhances wound healing and tissue regeneration in burn mice model

2019 ◽  
Vol 14 (2) ◽  
pp. 101-106
Author(s):  
Zainab Qureshi ◽  
Taous Khan ◽  
Abdul Jabbar Shah ◽  
Fazli Wahid
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Histological Analysis ◽  
Negative Control ◽  
Wound Contraction ◽  
Mice Model ◽  
Wound Area ◽  
Solanum Incanum ◽  
Partial Thickness ◽  
Partial Thickness Burn

This study was conducted to evaluate the topical efficacy of Solanum incanum for the treatment of partial-thickness burn in mice model. Mice were treated with topical ointment of S. incanum three times daily for 14 days. The wound healing was observed through wound contraction and histological parameters. The group treated with S. incanum ointment showed 81% reduction in wound area as compared to negative control where wound area reduced to 22%. The histological analysis further confirmed that ointment favors the tissue regeneration and reepithelization thus heal wound rapidly as com-pared to other groups. In conclusion, S. incanum extract enhances wound healing and tissue regeneration.

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