scholarly journals In-Vivo Neuropharmacological and Anti-Nutrient Study of Methanolic Extract of Lemna Minor

2021 ◽  
pp. 1-6
Author(s):  
Md Shahidul Islam ◽  
◽  
Debapriya Mazumder ◽  
Keyword(s):  
Methanolic Extract ◽  
Raw Materials ◽  
Lemna Minor ◽  
Dry Weight ◽  
Biological Evaluation ◽  
Total Phenolic ◽  
Weight Content ◽  
Soxhlet Apparatus ◽  
Test Hole

Medicinal plants constitute an important natural wealth of a country. They play a significant role in providing primary health care services to rural people. They serve as therapeutic agents as well as important raw materials for the manufacture of traditional and modern medicine. Lemna minor, the common duckweed is an aquatic freshwater aquatic plant of the genus Lemna. They belong to the family of Lemnaceae, which is monophyletic to the Araceae family. The study assesses In-vivo Neuropharmacological and Anti Nutrient Study of methanolic extract of Lemna minor. The plants were collected from Chandpur were extracted in methanol solvents using soxhlet apparatus. Chemical tests to identify different anti nutrients, total phenolic and flavonids were carried out using specific reagents. Several test such as open field test, hole cross test, hole board test and phenobarbital induced sleeping time test are performed to check possible neurological activity.The study of anti-nutrient properties showed a very high amount of tannin (5.625gm/kg) but a low amount of phytic acid (0.03gm/kg) & alkaloids (0.526gm/kg) comparison with standard value. Total phenolic (19.22 GAE/g dry weight) and flavonoids (7.68 QE/g dry weight) content determine that Lemna minor have a very good amount of phenolic and flavanoids content and which may act as good anti-oxidant. In the study of in-vivo neurological activity the efficacy of Lemna minorextract was compared with standard anxiolytic drug diazepam (1 mg/kg b.w.) showed limited sedation as well as anxiolytic properties in mice. The present study of in-vivo & anti-nutrient is biological evaluation of this plant forms a primary platform for further phytochemical, anti-nutrient & pharmacological studies.

Antioxidant and Analgesic Activity of Acer laevigatum Wall, a Traditional Mizo-tribe Medicine

2020 ◽  
Vol 13 (6) ◽  
pp. 5200-5207
Author(s):  
Andrew Lalthasanga Ralte ◽  
Phaibiang Lapasam ◽  
Freddy Teilang Nongkhlaw ◽  
Pdiangmon Kyndait ◽  
Zothanpuia
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Analgesic Activity ◽  
Methanolic Extract ◽  
Bark Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
External Application ◽  
Soxhlet Apparatus

Acer laevigatum is an evergreen tree growing to a height of 10–15 m or more, with a trunk up to 50 cm diameter belonging to the family Sapindaceae. In Mizoram, the decoction of the leaves is used as an external application in sprains. Extraction was carried out by drying the leaves and barks and extracted by using methanol as solvent using the Soxhlet apparatus. Preliminary phytochemical screening was carried out by methanolic extract of both leaves and barks to determine the chemical constituents present in the plant using a different phytochemical test, acute toxicity for leave and bark extract, in-vitro antioxidant activity and in-vivo analgesic activity of barks extract. Phytochemical screening was performed for both extract and it contains glycoside, saponin, phenol, tannin, flavonoid, and steroid. The antioxidant activity test of the methanolic extract of bark extract was performed successfully. In acute toxicity, the LD50 was found that for more than 2000 mg/kg body weight was safe for further uses. The total phenolic content of the bark extract contains 493 ± 0.23 mg of GAE/g and the total flavonoids content of the bark extract was 220 ± 0.034 mg of QE/g. The IC50 value of DPPH free radical scavenging activity was found to be 86.1211 µg/ml and nitric oxide was 75.9 µg/ml. Whereas, in reducing power it was found that the percentage inhibition was increased with an increase in concentration (increase in concen-tration, percentage inhibition was also increased) and reduced Fe3+ (ferricyanide complex) to Fe2+ (ferrous form). Finally, for in-vivo analgesic activity, 4000 mg/kg was more effective than 2000 mg/kg of the bark extract. These results confirm that the methanolic extract of bark of Acer laevigatum possesses antioxidant activity and non-significant or less analgesic activity.

scholarly journals Hepatoprotective, Antioxidant, and Anticancer Effects of theTragopogon porrifoliusMethanolic Extract

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Clara Tenkerian ◽  
Mirvat El-Sibai ◽  
Costantine F. Daher ◽  
Mohamad Mroueh
Keyword(s):  
Antioxidant Activity ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Fold Increase ◽  
Dry Weight ◽  
Total Phenolic ◽  
Anticancer Activities

Tragopogon porrifolius(Asteraceae), commonly referred to as white salsify, is an edible herb used in Lebanese folk medicine to treat cancer and liver dysfunction. In this study, we investigated the antioxidant activity ofTragopogon porrifoliusmethanolic extract, bothin vitroandin vivo, in addition to its hepatoprotective and anticancer activities. Total phenolic and flavonoid contents were measured and found to be37.0±1.40 mg GAE/g and16.6±0.42 mg QE/g dry weight, respectively.In vitroantioxidant assays revealed an FRAP value of659±13.8 µmol Fe2+/g of extract and DPPH IC50value 15.2 µg/mL. In rats subjected to CCl4-induced hepatotoxicity, significant increase in CAT, SOD, and GST levels was detected. The highest dose of the extract (250 mg/kg) recorded a fold increase of 1.68 for SOD, 2.49 for GST, and 3.2 for CAT. The extract also showed substantial decrease in AST (57%), ALT (56%), and LDH (65%) levels. Additionally, the extract caused a dose-dependent decrease in cell viability and proliferation. In conclusion, the methanolic extract ofT. porrifoliusdisplayed a relatively high antioxidant activity bothin vitroandin vivoas well as hepatoprotective potential against liver toxicity in rats and anticancer effect on MDA-MB-231 and Caco-2 cells.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

scholarly journals Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2216 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Guo-Yi Tang ◽  
Qing Liu ◽  
Xiao-Yu Xu ◽  
Shi-Yu Cao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Herbal Tea ◽  
Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

Exploring the potential effect of Methanolic extract of Salvia officinalis against UV exposed skin aging: In vivo and In vitro model

2019 ◽  
Vol 12 ◽  
Author(s):  
Ruchi Khare ◽  
Neeraj Upmanyu ◽  
Megha Jha
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Treated Group ◽  
Salvia Officinalis ◽  
Antioxidant Potential ◽  
Meoh Extract ◽  
Soxhlet Apparatus

Context: The medicinal plants have enormous pharmacological properties and having fewer side effects. Today there is increasing demand of medicinal plants as an anti-aging and anti-wrinkle agent. Objective: The aim of this study is to evaluate antioxidant, anti-aging and anti-wrinkle potential of Salvia officinalis. Materials and Methods: Salvia officinalis (Lamiaceae) is folk medicine of Asia and Latin America. Powdered crude drug 100 g were successively extracted in a soxhlet apparatus with petroleum ether (60-80ºC), chloroform and methanol. After successive solvents extraction methanolic extract was used for testing of antioxidant potential using DPPH assay. Further, antiaging potential of extract was investigated by inhibitory effect of various enzymatic estimations i.e. Col-I, Ela-I and Hya-I inhibitory assays on early aging human skin fibroblasts. Antiwrinkle potential of plant Salvia officinalis was done by using UV light induced photo aging model. Results: Phytochemical analysis showed the presence of glycosides, alkaloids flavonoids, and triterpenoids, saponins and Phenolic compounds in high level. Extract showed inhibitory concentration (IC50:24.65) and ascorbic acid the standard antioxidant showed inhibitory concentration (IC50:20.10). In enzymatic estimations assay, the Col-I, Ela-I and Hya-I of extract were assessed showing inhibitory concentration as Col-I (IC50:21.36), Ela-I (IC50:35.05) and Hya-I (IC50:23.44) respectively. Thus, MeOH extract of Salvia officinalis able to inhibit 50% of the activity of aging related enzymes Col-I, Ela-I and Hya-I. The wrinkle score of negative control i.e. UV treated group was 2.83±0.408 and MeOH extract of Salvia officinalis treated group is 1.83 ±0.753. Conclusion: This study concluded that MeOH extract of Salvia officinalis has confirmed the high antioxidant potential and In vitro and In vivo inhibitory potential of antiaging enzymes assessed, thus they could be used for further development of cosmetic products and nutraceuticals.

scholarly journals Sorghum halepense (L.) Pers rhizomes inhibitory potential against diabetes and free radicals

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Muhammad Abdur Rehman Shah ◽  
Rahmat Ali Khan ◽  
Mushtaq Ahmed
Keyword(s):  
Free Radicals ◽  
Total Phenolic Content ◽  
Cardiac Glycosides ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Sorghum Halepense ◽  
Total Phenolic ◽  
Brine Shrimps

Abstract Background Owing to the side effects of synthetic medicine and less effectiveness against different syndromes, the researchers have focused on phytotherapy to overcome these problems. The purpose of this project was to study the in vitro phytochemical, cytotoxic, total phenolic, antioxidant and antidiabetic activities of the methanol extract of the rhizome of Sorghum halepense (L.) Pers and its n-hexane, chloroform and aqueous fractions. Thereafter, to conduct in vivo evaluation of the effective extract for its antidiabetic and antioxidant characteristics. Methods Cytotoxic, total phenolic content and antidiabetic properties were ascertained by brine shrimps lethality, Folin- Ciocalteu reagent and alpha-amylase inhibition assays respectively while antioxidant activities were investigated through DPPH, ABTS and H2O2 assays. The methanolic extract was assessed in vivo for its antidiabetic and antioxidant activities by using Wistar albino rats. Results The phytochemical investigation of the methanolic extract and its unlike fractions revealed the availability of alkaloids, cardiac glycosides, flavonoids, terpenes, steroids, carbohydrate and proteins while lack of saponins and gums in methanolic extract. Steroids and carbohydrates were only present in aqueous and chloroform fraction respectively while both fractions contained proteins and alkaloids. Cardiac glycosides and flavonoids were absent in aqueous and chloroform fractions respectively. The highest brine shrimps lethality (70.5 ± 1.2), total phenolic content (28.30 ± 1.3 mg GAE/g), free radicals scavenging potential i.e. DPPH (40.02%), ABTS (40.48%) and H2O2 (50.85%) and alpha amylase inhibition (61.87%) was shown by the methanolic extract. The in vivo results did not disclose any sign of acute toxicity. The diabetic control showed a noteworthy (P < 0.05) decline in weight, HDL and glutathione and a raised level of bilirubin, blood glucose, urea, creatinine, triglyceride, LDL, VLDL, ALT, ALP, AST, SOD, catalase. The mentioned alterations were restored considerably (P < 0.05) by treatment of diabetic rats with methanolic extract of Sorghum halepense (L.) Pers (150 and 300 mg/kg b.w.). Conclusion It is concluded that the extract of rhizomes of Sorghum halepense (L.) Pers is an effective fount of antioxidant and anti-diabetic compounds. Further analysis is needed to sharpen its pharmacological activities.

scholarly journals UNRIPE FRUIT OF RUBUS FRAXINIFOLIUS AS A POTENTIAL SOURCE OF ANTIOXIDANT AND ANTIELASTASE AGENT

2021 ◽  
pp. 78-81
Author(s):  
YESI DESMIATY ◽  
BERNA ELYA
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Flavonoid Content ◽  
Unripe Fruit ◽  
Potential Source ◽  
Soxhlet Apparatus ◽  
Anti Oxidant

Objective: This research aimed to examine the anti-oxidant activity, antielastase activity, and the content of total phenolic and total flavonoid of R. fraxinifolius unripe fruit. Methods: The dried unripe fruit was extracted using Soxhlet apparatus with sequence solvent: n-hexane, ethyl acetate, and methanol. Each extract was determined the anti-oxidant and antielastase activity, total phenolic, and total flavonoid content. Result: The result showed the extracts (n-hexane, ethyl acetate, and methanol) gave anti-oxidant IC50>200; 186.84; and 19.74 ppm, and the ability of elastase inhibition was 6.84+0.9%; 52.23+7.1%; and 57.81+5.5% at 100 ppm, respectively. The methanolic extract contained phenolic 202.2 mg GAE/g extract and flavonoid 43.89 mg QE/g extract. Conclusion: R. fraxinifolius unripe fruit has shown potential as a DPPH (α, α-diphenyl-β-picrylhydrazyl) radical scavenger and anti-elastase. This study provides an excellent effect to underline the importance of R. fraxinifolius unripe fruit, and it can be developed as nutricosmetics, nutraceuticals, or herbal anti-wrinkle cosmetics.

scholarly journals COMPARATIVE STUDIES OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF IN VIVO PLANT AND IN VITRO CALLUS EXTRACT OF CARDIOSPERMUM HALICACABUM L.

2021 ◽  
pp. 94-103
Author(s):  
THANIARASU R ◽  
LOGESHWARI M
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Biologically Active ◽  
Diffusion Method ◽  
Total Phenolic

Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.

scholarly journals Antioxidant and Antimicrobial Activity of Cleome droserifolia (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity and Gut Microflora

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1929
Author(s):  
Nesrein M. Hashem ◽  
Mohamed G. Shehata
Keyword(s):  
Phenolic Compounds ◽  
Blood Plasma ◽  
Methanolic Extract ◽  
Biological Effects ◽  
Redox Status ◽  
Biological Evaluation ◽  
Biologically Active ◽  
Lactobacillus Species

This study aimed to investigate the antioxidant, antimicrobial, and immunomodulatory activities of a Cleome droserifolia (Forssk.) Del. (Cd) shoot methanolic extracts considering the biological activity of its phytogenic compounds. For this purpose, the Cd phenolic compounds were detected, and an in vitro evaluation of the antioxidant and antimicrobial activities of the Cd extract was performed. For a biological evaluation, 30 v-line rabbits were randomly distributed into three groups with treatments including: a basal diet without Cd shoots powder supplement (C group) or supplemented with 1.25- (Cdl group) or 2.5 (Cdh group)-mg Cd/kg dry matter (DM). The Cd extract showed a linear scavenging activity for 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), with the maximal activity observed at a concentration of 1 mg/mL. A total of 16 phenolic compounds were identified by reverse-phase high-performance liquid chromatography (RP-HPLC) in the Cd methanolic extract, among which benzoic acid, rutin, ellagic acid, naringenin, and o-coumaric acid were the major compounds. The methanolic extract of Cd showed inhibitory actions against microbial pathogen species. The in vivo study showed that the two concentrations of Cd significantly improved the redox status of the blood plasma and lysozyme activity. Treatment with Cdh significantly decreased the levels of interleukin-β1 in the blood plasma compared with the control. Moreover, the two concentrations of Cd significantly increased the counts of intestinal and cecal yeast and Lactobacillus species and decreased the Salmonella and Coliform species compared with the control. The aerial parts of the Cd shrub had strong antioxidant, antimicrobial, and immunomodulatory activities, which can improve the overall health status and seem to be related to its impressive range of biologically active phenolic compounds.

scholarly journals Three Types of Red Beetroot and Sour Cherry Based Marmalades with Enhanced Functional Properties

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5090
Author(s):  
Oana Viorela Nistor ◽  
Liliana Șeremet (Ceclu) ◽  
Gabriel Dănuț Mocanu ◽  
Vasilica Barbu ◽  
Doina Georgeta Andronoiu ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Raw Materials ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sour Cherry ◽  
Dry Weight ◽  
Added Value ◽  
Confocal Laser ◽  
Total Phenolic ◽  
Before And After

The importance of bioactive compounds such as betalains and anthocyanins was highlighted in the present study by the valorization of red beetroot and sour cherry as an attempt to develop healthy products. The aim of the study was to obtain and characterize three types of marmalade based on red beetroot, sour cherry and both in 1:1 combination, obtained by heating at 95 °C for 30 min. Changes in total phenolic content, total flavonoids, betalains, anthocyanins and antioxidant activity were evaluated before and after the thermal treatment. Several other analyses such as color, rheological and textural analyses and confocal laser microscopy were performed to provide further information about the quality of the added-value food products. A significant decrease of 34% in betalains content was registered in the red beetroot marmalade due to the chemical changes in bioactives induced by the temperature. A satisfactory ABTS radical scavenging activity of 8.12 ± 0.38 mMol Trolox/g dry weight (dw) was obtained for the red beetroot and sour cherry based marmalade. The gelled structure was validated by the rheological and textural characteristics. The results highlighted the potential use of red beetroot and sour cherry as food raw materials, due to their particular profile of bioactive compounds.

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