scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL POTENTIAL OF ZANTHOXYLUM ALATUM FRUIT AND LEAVES EXTRACTS AGAINST SELECTED PATHOGENIC BACTERIA

2021 ◽  
Vol 37 (1) ◽  
pp. 36-41
Author(s):  
A. Sharif ◽  
H. Javed ◽  
A. Ali ◽  
I. Ahmed ◽  
F. N. Khoso
Keyword(s):  
Antioxidant Activity ◽  
Liquid Medium ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Fruit Extract ◽  
E Coli ◽  
Medical Needs ◽  
Ic50 Values ◽  
Zones Of Inhibition ◽  
Antibacterial Potential

Zanthoxylum alatum (Z. alatum), an important medicinal plant is used for various ailments including chest infection, cough, cholera, fever, stomach disorders, gas problems, indigestion, piles, toothache, gum problems, dyspepsia and stomachic worldwide. Keeping in view the medicinal potential of this plant, fruit and leaves methanolic (MeOH) extracts were prepared, evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and for antibacterial potential by well diffusion and macrodilution methods in-vitro. Our results confirmed that Z. alatum fruit and leaves extracts have significant antioxidant activity with IC50 values 0.28 ± 0.07 mg/ml and 0.34 ± 0.05 mg/ml, respectively. The inhibitory trend at highest tested concentration (120 mg/ml) at 24 hr incubation in well diffusion method was recorded as 0.39, 0.30, 0.28, 0.27 and 0.18 cm against S. pyogenes, B. cereus, E. coli, S. aureus and S. enterica for leaf extract, respectively. In case of fruit extract inhibitory trend at highest tested concentration was observed as 0.33, 0.32, 0.31, 0.30 and 0.28 cm against S. aureus, S. enterica, S. pyogenes, B. cereus and E. coli, respectively. The fruit extract showed higher zones of inhibition than leaves extracts against all the test bacteria except S. pyogenes. Moreover, highest zones of inhibition were observed at lowest incubation (24 hr) and lowest zones were observed at highest incubation period (72 hr) for all tested concentrations. Macrodilution method showed antibacterial susceptibility in liquid medium with different levels of IC50 values ranging from 1.6±0.13 mg/ml to 10.3±0.7 5mg/ml. Interestingly, none of the tested bacteria showed resistance against any of the test extract in well diffusion or macrodilution method expressing the Z. alatum as potent candidates to kill bacteria in semisolid or in liquid medium to fulfill medical needs in future.

scholarly journals Antibacterial Potential of Ethanolic and Aqueous Extracts of Carica papaya Leaves

2020 ◽  
pp. 33-38 ◽  
Author(s):  
Augustine I. Airaodion ◽  
John A. Ekenjoku ◽  
Ime U. Akaninyene ◽  
Anthony U. Megwas
Keyword(s):  
Aqueous Extract ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Filter Paper Disc ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Antibacterial Potential

Background: The search for newer sources of antibiotics is a global challenge pre-occupying research institutions, pharmaceutical companies and academia, since many infectious agents are becoming resistant to synthetic drugs. Aim: This present study sought to investigate the antibacterial potential of ethanolic and aqueous extracts of Carica papaya leaves. Materials and Methods: Fresh and healthy leaves of C. papaya were harvested, air dried and milled into powder. The powder was extracted using ethanol and water as solvents. The antibacterial activities of both extracts were determined by diffusion method. Nutrient agar medium was prepared using standard method. Pure cultures of Coliform bacillus, Staphylococcus epidermidis, Streptococcus viridans, Salmonella typhi and Escherichia coli were obtained from the Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria. The extracts were serially diluted to obtain 1.0%, 0.5%, 0.25% and 0.125% solutions in sterile test tubes. Sterilized 9 mm filter paper disc soaked in the diluted extracts were placed on the plate and incubated for 24 hours at room temperature. The plates were examined for clear zones of inhibition. Presence of zones of inhibition indicated activity. Results: the results showed that both ethanolic and aqueous extracts of C. papaya leaves exhibit antibacterial activities against C. bacillus, S. epidemidis, S. viridans and E. coli and also inhibited their growth. The effect of the ethanolic extract was greater than that of the aqueous extract. However, this activity was not observed with S. typhi. Conclusion: The result of the present study showed that C. papaya leaves might effectively inhibit the growth of C. bacillus, S. epidemidis, S. viridans and E. coli but not that of S. typhi. However, the ethanolic extract is more potent than the aqueous extract.

scholarly journals Analisa Potensi Ekstrak Daun Kari (Murraya koenigii L. Spreng)

2019 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Mustanir Mustanir ◽  
Tara Rizki Al-Qarana ◽  
Hilda Gusvianna ◽  
Nurdin Saidi
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Murraya Koenigii ◽  
E Coli ◽  
Ic50 Values ◽  
Hexane Extracts ◽  
Inhibition Zones

Daun M. koenigii sangat umum digunakan sebagai rempah dalam berbagai masakan di Aceh, namun pemahaman masyarakat terhadap pentingnya daun M. koenigii perlu ditingkatkan agar pemanfaatannya dapat ditingkatkan. Dalam penelitian ini dilakukan uji aktivitas antibakterial daun M. koenigii terhadap bakteri E. coli dan S. aureus serta uji aktivitas antioksidan terhadap radikal bebas DPPH (1,1-Diphenyl-2-pycrilhydrazil). Uji antibakterial dilakukan dengan metode difusi cakram, dan hasilnya ekstrak etil asetat menghasilkan zona hambat yang lebih besar dibandingkan ekstrak metanol dan n-heksana. Zona hambat yang dihasilkan pada konsentrasi 1, 5 dan 10 % secara berturut-turut adalah 8,7; 7,7 dan 6,7 mm terhadap E. coli, dan 12,8 ;10,7 dan 8,0 mm terhadap S. aureus. Ekstrak etil asetat memiliki aktivitas antioksidan paling kuat dibandingkan dengan ekstrak metanol dan nheksana secara berturut-turut berdasarkan nilai IC50 yaitu 23; 50,54; dan 64,70 ppm. Berdasarkan kromatografi kolom diperoleh 8 (A-H) subfraksi dan subfraksi G menunjukan aktifitas antioksidan yang paling kuat sebesar 14,41 ppm   Murraya koenigii (M. koenigi) leaves are very commonly used as spices in various cuisines in Aceh, but people's understanding of the importance of M. koenigi leaves needs to be improved, so it can be utilized better. In this study, the antibacterial activity of M. koenigii leaves was carried out against E. coli and S. aureus and antioxidant activity against DPPH free radicals (1,1- Diphenyl-2-pycrilhydrazil). Antibacterial tests were carried out using the disc diffusion method, and as a result, the ethyl acetate extract produced a larger inhibition zone than the methanol and n-hexane extracts. The inhibition zones produced at concentrations of 1, 5 and 10% were 8.7; 7.7 and 6.7 mm against E. coli, and 12.8; 10.7 and 8.0 mm against S. aureus. Ethyl acetate extract had the strongest antioxidant activity compared to methanol and nexexane extracts based on IC50 values, 23; 50; 54; and 64.70 ppm. Based on column chromatography obtained, 8 (A-H) subfraction and subfraction G showed the strongest antioxidant activity of 14.41 ppm.

scholarly journals Euphorbia graminea Jacq. (Euphorbiaceae): The antimicrobial assessment of the extract and fractions of the leaves

2020 ◽  
pp. 3809-3818
Author(s):  
Ikpefan E. O. ◽  
Enwa F. O. ◽  
Emebrado O.
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Staphyloccocus Aureus ◽  
Test Organisms ◽  
Zones Of Inhibition ◽  
Bacteria And Fungi

This study was carried out as a result of the belief that certain medicinal plants have antimicrobial activity against pathogenic bacteria and fungi species, hence the in vitro antimicrobial activity of the extract and fractions of Euphorbia graminea was performed against bacteria (Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa) and fungal (Candida albican) non-clinical isolates. The methanol extract of Euphorbia graminea was fractionated via solvent-solvent partitioning and vacuum liquid chromatographic techniques and the corresponding fractions were tested for phytochemicals and were biologically tested against the organisms employing the agar well diffusion method. While the extract and partitioned fractions were tested at concentrations between 4.69-300 mg/mL, the vlc subfractions were tested at 12.50-200 mg/mL against the organisms. The MIC of the active vlc subfractions was also tested (9.38-37.5 mg/mL). The alkaloids as well as glycosides, tannins, terpenes and steroids were detected among the extracts and fractions of E. graminea. The extract showed mild activities against the test organisms with the highest zone of inhibition of 7.00 mm recorded at 300 mg/mL against S.aureus. The activities of both extract at 300 mg/mL, showed notable increase against the organisms used, with zones of inhibition of the aqueous extract been 10.50 and 9.50mm and for the chloroform extract, 16.50 and 13.05mm (S. aureus and E. coli respectively). The vlc sub-fraction 6-8 (C) among the other subfractions was more potent against S. aureus, E. coli and C. albican as 16.50, 12.00 and 0.50 mm zones of inhibition were recorded at 100 mg/mL. The zones of inhibition against these organisms later increased to 20.00, 18.50 and 15.00 mm at 200 mg/mL. This study has highlighted the fact that the plant E. graminea has antimicrobial activity which occurs more in the partitioned chloroform and its chromatographic vacuum liquid subfraction (6-8) that gave the highest activity. However, in order to isolate the active biological components and to determine their safety in drug production, further studies are needed.

scholarly journals Antagonistic effects of raffia sap with probiotics against pathogenic microorganisms

2020 ◽  
pp. 24-31
Author(s):  
Manga Joseph Arsene Mbarga ◽  
Steve Carly Zangue Desobgo ◽  
Léopold Ngoune Tatsadjieu ◽  
Nyasha Kavhiza ◽  
Louis Kalisa
Keyword(s):  
Mathematical Models ◽  
Incubation Time ◽  
Pathogenic Bacteria ◽  
Antagonistic Activity ◽  
Bifidobacterium Bifidum ◽  
Antagonistic Effect ◽  
Diffusion Method ◽  
Study Objective ◽  
E Coli ◽  
Zones Of Inhibition

Introduction. Probiotics are known for their beneficial properties. Numerous studies have been conducted to find advantages that probiotics can provide. This study aimed to evaluate the functional properties of raffia sap, a Cameroonian drink, fermented with probiotics by investigating its antagonistic activity against pathogenic bacteria. Study objects and methods. The study objective was raffia sap fermented by Lactobacillus fermentum and Bifidobacterium bifidum. Box-Behnken design with four factors (seeding rates of L. fermentum and B. bifidum, temperature, and incubation time) was used to generate mathematical models. The disc diffusion method was used to evaluate an antagonistic effect of the probiotics against four pathogenic bacteria (Escherichia coli, Listeria monocytogenes, Salmonella sp., and Bacillus cereus). An optimization of mathematical models of the inhibition diameters allowed to determine the optimal conditions of antagonistic effect. Results and discussion. The experimental data showed that zones of inhibition were 0‒21 mm for Salmonella sp., 0‒23 mm for E. coli, 0‒20 mm for L. monocytogenes, and 0‒22 mm for B. cereus. ANOVA results and the mathematical models obtained showed that L. fermentum was effective against B. cereus and B. bifidum against Salmonella sp., E. coli, and B. cereus. The optimization of the models revealed maximum zones of inhibition at the seeding rates of L. fermentum and B. bifidum of 2 and 10%, respectively, incubation time of 48 h, and temperature of 37°C. Conclusion. Raffia sap fermented by L. fermentum and B. bifidum demonstrated antagonistic effect against pathogenic bacteria such as E. coli, L. monocytogenes, Salmonella sp., and B. cereus.

Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

1970 ◽  
Vol 9 (2) ◽  
pp. 3212-3214
Author(s):  
Pramod Dhakal ◽  
Ankit a Achary ◽  
Vedamurthy Joshi
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Watery Diarrhea ◽  
Diffusion Method ◽  
E Coli ◽  
Drug Molecules ◽  
Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

scholarly journals Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
Pattamaporn Aksornchu ◽  
Netima Chamnansilpa ◽  
Sirichai Adisakwattana ◽  
Thavaree Thilavech ◽  
Charoonsri Choosak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Digestive Enzyme ◽  
Radical Scavenging ◽  
Protein Glycation ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Values

Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR

2020 ◽  
pp. 78-84
Author(s):  
FAVIAN BAYAS-MOREJON ◽  
ANGELICA TIGRE ◽  
RIVELINO RAMON ◽  
DANILO YANEZ
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Potassium Phosphate ◽  
Natural Antioxidants ◽  
Butylated Hydroxytoluene ◽  
Penicillin G ◽  
Diffusion Method ◽  
E Coli ◽  
Antibacterial And Antioxidant Activity

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.

Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Diffusion Method ◽  
Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF COCOA POD THAT ASSOCIATED IN MALTODEXTRIN IN VARIOUS CONCENTRATION

2018 ◽  
Vol 5 (2) ◽  
pp. 123
Author(s):  
Asriani Hasanuddin ◽  
Chairil Anwar ◽  
Marhawati Mappatoba ◽  
Hafsah Hafsah
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Natural Food ◽  
Skin Extract ◽  
E Coli ◽  
Dpph Method ◽  
Antioxidant And Antimicrobial Activity ◽  
Peel Extract

Cocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives.

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