EVALUATION OF THE HAEMOSTATIC POTENTIALS OF CRUDE METHANOLIC LEAF EXTRACT OF PERSEA AMERICANA IN WISTAR RATS

Background: Tooth extraction, a common practice among the dental profession, causes trauma to the blood vessels during the wound healing process. The acceleration of wound healing, within which fibroblasts play an important role, is influenced by nutrition. Avocado leaves contain a variety of chemicals, including flavonoid compounds, tannins, katekat, kuinon, saponin and steroids/triterpenoid. Avocado leaves also contain glycosides, cyanogenic, alkaloids and phenols which function as anti-inflammatory, antibacterial and antioxidant agents. This avocado leaf content could be used as an alternative medicine to accelerate the wound healing process in post-tooth extraction sockets. Purpose: To determine the role of avocado leaves (Persea americana Mill) in accelerating fibroblast cells proliferation in tooth socket post-extraction. Methods: The sample was divided into four groups, a control group and three treatment groups. The treatment groups used avocado leaf extract and 3% CMC Na solution which was inserted into the tooth sockets of Wistar rats. Both the control and treatment groups had their mandibula decapitated with all the required specimens being prepared on the 3rd and 7th days of the experiment. Mandibular decapitation and tooth extraction socket were prepared by HPA (Histology Pathology Anatomy) with Hematoxylin Eosin (HE) staining. The fibroblast proliferation was analyzed by means of a light microscope at 400x magnification. The obtained data was analyzed using a t-Test. Result: The t-Test obtained a significance value 0.001 (p <0.05) between the control and treatment groups. The number of fibroblast cells increased in the group treated on the third day and decreased in the group treated on the seventh day. Conclusion: Avocado leaf extract (Persea americana Mill.) accelerates proliferation of fibroblast cells in Wistar rats post-tooth extraction.

Download Full-text

ANTI-ESTROGENIC PROPERTIES OF ETHANOLIC LEAF EXTRACT OF BOERHAVIA DIFFUSA ON FEMALE WISTAR RATS.

10.1055/s-0037-1608549 ◽

2017 ◽

Author(s):

C Olajide Omolola ◽

J Okpuzor ◽

I Nnadozie Jane ◽

R Adewole Adeniyi

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Female Wistar Rats ◽

Estrogenic Properties ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

Download Full-text

Effects of n-hexane and ethylacetate fractions of Terminalia catappa leaf extract in experimental Trypanosoma brucei brucei infection in Wistar rats

Comparative Clinical Pathology ◽

10.1007/s00580-020-03192-y ◽

2021 ◽

Author(s):

Folashade Sarah Ojeleye ◽

Helen Ileigo Inabo ◽

Clement Myah Zaman Whong ◽

Bolanle Olufunke Priscilla Musa ◽

Ochuko Orakpoghenor

Keyword(s):

Trypanosoma Brucei ◽

Wistar Rats ◽

Leaf Extract ◽

Trypanosoma Brucei Brucei ◽

Terminalia Catappa

Download Full-text

Effect of hydro-alcoholic root extract of Plumbago zeylanica l alone and its combination with aqueous leaf extract of Camellia sinensis on haloperidol induced parkinsonism in wistar rats

Annals of Neurosciences ◽

10.5214/ans.0972.7531.210204 ◽

2014 ◽

Vol 21 (2) ◽

Cited By ~ 2

Author(s):

Sibi P Ittiyavirah ◽

Ruby R

Keyword(s):

Camellia Sinensis ◽

Wistar Rats ◽

Leaf Extract ◽

Root Extract ◽

Plumbago Zeylanica ◽

Aqueous Leaf Extract

Download Full-text

Subacute effect of Vernonia amygdalina methanolic crude leaf extract on the hematological profile in albino Wistar rats

Nigerian Journal of Experimental and Clinical Biosciences ◽

10.4103/2348-0149.123963 ◽

2013 ◽

Vol 1 (1) ◽

pp. 43

Author(s):

Thomas Nubila ◽

NkoyoImelda Nubila ◽

Ayodele Ukaejiofo ◽

ElvisNeba Shu ◽

ChukwubuzorU Okwuosa ◽

...

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Vernonia Amygdalina ◽

Hematological Profile ◽

Crude Leaf Extract

Download Full-text

Antidiarrheal Activity of Dissotis multiflora (Sm) Triana (Melastomataceae) Leaf Extract in Wistar Rats and Subacute Toxicity Evaluation

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/4038371 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Alian Désiré Afagnigni ◽

Maximilienne Ascension Nyegue ◽

Chantal Florentine Ndoye Foe ◽

Youchahou Njankouo Ndam ◽

Frédéric Nico Njayou ◽

...

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Shigella Flexneri ◽

Female Rats ◽

Male Rats ◽

Dependent Manner ◽

Subacute Toxicity ◽

Antidiarrheal Activity ◽

Dose Dependent ◽

Ethanolic Leaf Extract

The present work was undertaken to evaluate antidiarrheal activity of ethanolic leaf extract of Dissotis multiflora (Sm) Triana (D. multiflora) on Shigella flexneri-induced diarrhea in Wistar rats and its subacute toxicity. Diarrhea was induced by oral administration of 1.2 × 109 cells/mL S. flexneri to rats. Antidiarrheal activity was investigated in rats with the doses of 111.42 mg/kg, 222.84 mg/kg, and 445.68 mg/kg. The level of biochemical parameters was assessed and organs histology examined by 14 days’ subacute toxicity. S. flexneri stool load decreased significantly in dose-dependent manner. The level of ALT increased (p<0.05) in male rats treated with the dose of 445.68 mg/kg while creatinine level increased in rats treated with both doses. In female rats, a significant decrease (p<0.05) of the level of AST and creatinine was noted in rats treated with the dose of 222.84 mg/kg of D. multiflora. Histological exams of kidney and liver of treated rats showed architectural modifications at the dose of 445.68 mg/kg. This finding suggests that D. multiflora leaf extract is efficient against diarrhea caused by S. flexneri but the treatment with doses lower than 222.84 mg/kg is recommended while further study is required to define the exact efficient nontoxic dose.

Download Full-text

Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2015-0049 ◽

2016 ◽

Vol 27 (2) ◽

Cited By ~ 16

Author(s):

Ganiyu Oboh ◽

Veronica O. Odubanjo ◽

Fatai Bello ◽

Ayokunle O. Ademosun ◽

Sunday I. Oyeleye ◽

...

Keyword(s):

Leaf Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Seed Extract ◽

Persea Americana ◽

Dependent Manner ◽

Natural Treatment

AbstractAvocado pear (The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of FeThe extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID.The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.

Download Full-text

acute and sub-acute toxicity studies of aqueous leaf extract of launaea taraxacifolia (willd.) in wistar rats

Journal of Complementary Medicine Research ◽

10.5455/jcmr.2021.12.01.11 ◽

2021 ◽

Vol 12 (1) ◽

pp. 95

Author(s):

Millicent Umaru ◽

Kabiru Abubakar ◽

Gabriel Uyaiabasi ◽

Giaze Tijjani ◽

Yusuf Alkali ◽

...

Keyword(s):

Acute Toxicity ◽

Wistar Rats ◽

Leaf Extract ◽

Toxicity Studies ◽

Aqueous Leaf Extract

Download Full-text

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

Download Full-text