scholarly journals Germination, Morphological and Physiological Evaluation of Seedlings Pretreated with Colchicine in Soybean (Glycine max L)

2021 ◽  
Vol 18 (18) ◽  
Author(s):  
Phetole MANGENA

Wider genetic diversity has the potential to improve crop productivity of soybean, especially under environmental stress conditions. The pre-treatment of soybean seeds with antimitotic agents to establish improved genetic pool may also contribute to the enhancement of germination, seedling development, morpho-physiological growth and yield. In this study, 2 soybean genotypes viz. TGx1835-10E and Dundee were imbibed in solutions containing different amounts of colchicine (0.0, 0.1, 0.5 and 1 %) to evaluate the variations in germination, morphometric and physiological parameters. The seeds were imbibed for the period of 12 and 24 h before sowing for germination in plastic pots containing moistened sterile vermiculite. The variance components expressed as means, and mean percentage of total variations showed that colchicine concentration and imbibitional duration were the most important sources of variation for all traits, followed by the genotypes. Significant responses were detected for various germination parameters, seedling morphology and physiological contents such as; chlorophyll content, total phenolics, flavonoids as well as total protein and DNA content in the 2 genotypes used. HIGHLIGHTS Mutagenic pre-treatment of seeds via imbibition Germination and seedling growths of colchicine preconditioned seeds Chloroplastidic pigment analysis of pre-treated seedlings Physiological valuation of primary and secondary metabolites in grown seedlings Role of colchicine on germination, seedling development and growth of soybean plantlets GRAPHICAL ABSTRACT

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jinyu Zhang ◽  
Huanqing Xu ◽  
Yuming Yang ◽  
Xiangqian Zhang ◽  
Zhongwen Huang ◽  
...  

Abstract Background Phosphorus (P) is essential for plant growth and development, and low-phosphorus (LP) stress is a major factor limiting the growth and yield of soybean. Long noncoding RNAs (lncRNAs) have recently been reported to be key regulators in the responses of plants to stress conditions, but the mechanism through which LP stress mediates the biogenesis of lncRNAs in soybean remains unclear. Results In this study, to explore the response mechanisms of lncRNAs to LP stress, we used the roots of two representative soybean genotypes that present opposite responses to P deficiency, namely, a P-sensitive genotype (Bogao) and a P-tolerant genotype (NN94156), for the construction of RNA sequencing (RNA-seq) libraries. In total, 4,166 novel lncRNAs, including 525 differentially expressed (DE) lncRNAs, were identified from the two genotypes at different P levels. GO and KEGG analyses indicated that numerous DE lncRNAs might be involved in diverse biological processes related to phosphate, such as lipid metabolic processes, catalytic activity, cell membrane formation, signal transduction, and nitrogen fixation. Moreover, lncRNA-mRNA-miRNA and lncRNA-mRNA networks were constructed, and the results identified several promising lncRNAs that might be highly valuable for further analysis of the mechanism underlying the response of soybean to LP stress. Conclusions These results revealed that LP stress can significantly alter the genome-wide profiles of lncRNAs, particularly those of the P-sensitive genotype Bogao. Our findings increase the understanding of and provide new insights into the function of lncRNAs in the responses of soybean to P stress.


2021 ◽  
Vol 883 (1) ◽  
pp. 012052
Author(s):  
N Herawati ◽  
A R Aisah ◽  
I Mardian ◽  
B N Hidayah ◽  
B T R Erawati

Abstract Plant growth is influenced by seed quality. Seeds need to be treated to prevent pest and disease disorders or to increase seed germination. Soybean planting was carried out in this study by the treatment of varieties and seed pre-treatment application before planting. The study aimed to measure the growth and yield of soybeans by varieties and seed treatment before planting, carried out in the Village of Nggembe, District of Bolo, Bima Regency. The study used factorial randomized block design with two factors. The first factor was soybean varieties consisting of two levels, namely Devon and Dena varieties, and the second factor was the application of pre-planting seed treatments consisting of four levels namely Cruiser, Marshall, Agrisoy, and without seed treatment, each treatment was repeated three times. Observations were made on the performance of agronomic crops and soybean yields. The results showed that the treatment of soybean varieties and the application of seed treatment had a significant influence on plant height and soybean productivity, and both treatments had interactions on parameters of plant height, number of filled pods, weight of 100 seeds, and soybean productivity with the best results successively produced by a combination of Devon-Control, Devon-Marshall, Devon-Agrisoy, and Dena-Cruiser.


2016 ◽  
Vol 4 (3) ◽  
pp. 121
Author(s):  
Amir Zaman Khan

Exploring ways to improve stand establishment and crop productivity under abiotic stresses like drought is important. Two years experiments were conducted at University of Agriculture, Peshawar-Pakistan to examine the efficacy of six pre-sowing seed hardening agents. Seeds of wheat cultivar Uqab-2000 were hardened in six different chemicals of various concentration viz; PEG-8000 (10%), CaCl2 (4%), KNO3, (3%), Mannital (4%), NaCl (5%), Na2SO4 (2%) along with water soaking and dry seeds as control for 24 hours and drying back to original moisture content at room temperature. The soaking and drying of seeds was repeated twice for 12 hours. The results showed that pre-sowing hardening of seed with PEG-8000, CaCl2 and KNO3 gave higher germination, decreased days to 50% germination, increased shoot length, root length, seedling fresh and dry weight in laboratory experiment as compared with other hardening and control treatment. Under field conditions, maximum plant height (93.53cm), spikelet’s spike-1 (17.16), grains spike-1 (50.82), 1000 grain weight (39.97 g), grain yield (3482 kg ha-1) and maximum harvest index (32.5%) were observed in PEG-8000 hardened seed than control treatment (2872 kg ha-1). Seed hardened in PEG-8000, CaCl2 and KNO3 gave 30% increase in grain yield as compared to Mannital, NaCl and Na2SO4 which gave 15% increase in grain yield over control treatment.


2018 ◽  
Vol 21 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Naoki Matsuo ◽  
Tetsuya Yamada ◽  
Yoshitake Takada ◽  
Koichiro Fukami ◽  
Makita Hajika

Metabolites ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 335 ◽  
Author(s):  
Sukhmanpreet Kaur ◽  
Vidya Suseela

Arbuscular mycorrhizal fungi (AMF) is among the most ubiquitous plant mutualists that enhance plant growth and yield by facilitating the uptake of phosphorus and water. The countless interactions that occur in the rhizosphere between plants and its AMF symbionts are mediated through the plant and fungal metabolites that ensure partner recognition, colonization, and establishment of the symbiotic association. The colonization and establishment of AMF reprogram the metabolic pathways of plants, resulting in changes in the primary and secondary metabolites, which is the focus of this review. During initial colonization, plant–AMF interaction is facilitated through the regulation of signaling and carotenoid pathways. After the establishment, the AMF symbiotic association influences the primary metabolism of the plant, thus facilitating the sharing of photosynthates with the AMF. The carbon supply to AMF leads to the transport of a significant amount of sugars to the roots, and also alters the tricarboxylic acid cycle. Apart from the nutrient exchange, the AMF imparts abiotic stress tolerance in host plants by increasing the abundance of several primary metabolites. Although AMF initially suppresses the defense response of the host, it later primes the host for better defense against biotic and abiotic stresses by reprogramming the biosynthesis of secondary metabolites. Additionally, the influence of AMF on signaling pathways translates to enhanced phytochemical content through the upregulation of the phenylpropanoid pathway, which improves the quality of the plant products. These phytometabolome changes induced by plant–AMF interaction depends on the identity of both plant and AMF species, which could contribute to the differential outcome of this symbiotic association. A better understanding of the phytochemical landscape shaped by plant–AMF interactions would enable us to harness this symbiotic association to enhance plant performance, particularly under non-optimal growing conditions.


2006 ◽  
Vol 20 (1) ◽  
pp. 172-178 ◽  
Author(s):  
Karen A. Zuver ◽  
Mark L. Bernards ◽  
James J. Kells ◽  
Christy L. Sprague ◽  
Case R. Medlin ◽  
...  

Herbicide-resistant corn hybrids offer additional options for POST weed control in corn, and growers may benefit from information on the consistency of these weed-control strategies. Studies were conducted in Indiana, Illinois, Michigan, and Ohio, in 2000 and 2001, to evaluate weed control among herbicide strategies for imidazolinone-resistant, glufosinate-resistant, glyphosate-resistant, and conventional corn. Isogenic hybrids were utilized to minimize variation in growth and yield potential among hybrids. The glyphosate-resistant corn postemergence (glyphosate-POST) treatment provided more consistent control of giant foxtail than the PRE, conventional corn postemergence (conventional-POST), glufosinate-resistant corn postemergence (glufosinate-POST), and imidazolinone-resistant corn postemergence (imi-POST) treatments. All four POST treatments were more consistent and provided greater control than the PRE treatment of the large-seeded broadleaf weeds velvetleaf, giant ragweed, common cocklebur, and morningglory species. Conventional-POST and imi-POST were more consistent than glufosinate-POST and glyphosate-POST treatments in controlling giant ragweed. There were no statistical differences in the variability of PRE or POST treatments for control of common lambsquarters, common ragweed, and redroot pigweed. Corn yield varied among locations and years. The glyphosate-POST treatment did not reduce yield relative to the weed-free treatment, the imi-POST and glufosinate-POST treatments each reduced yield in one of eight locations, and the conventional-POST treatment reduced yield in three of eight locations.


2016 ◽  
Vol 67 (10) ◽  
pp. 1096 ◽  
Author(s):  
Vengavasi Krishnapriya ◽  
Renu Pandey

High-molecular-weight secretory proteins and low-molecular-weight exudates (carboxylates, phenols, free amino acids and sugars) released from roots of soybean (Glycine max (L.) Merr.) differentially influence genotypic phosphorus (P) acquisition efficiency (PAE). We hypothesised that genotypes with higher root exudation potential would exhibit enhanced P acquisition, and screened 116 diverse soybean genotypes by labelling shoots with 14CO2. A root exudation index (REI) derived from total 14C in the root exudate at sufficient (250 μm) and low (4 μm) P levels was used to classify genotypes for PAE. Genotypes with REI >2.25 exhibited significantly higher exudation at low than at sufficient P, which in turn increased PAE. Under low P availability, efficient genotypes exude a greater quantity of organic compounds into the rhizosphere. This increases P availability to meet the crop requirement, enabling the crop to produce consistent biomass and seed yield with reduced fertiliser addition. Such maintenance of growth and yield potential by mining the inherent soil P is a favourable trait in genotypes, reducing dependence on P fertilisers. Measuring REI at seedling stage to select P-efficient plants accelerates the screening process by accommodating large numbers of genotypes.


Revista CERES ◽  
2013 ◽  
Vol 60 (5) ◽  
pp. 621-628 ◽  
Author(s):  
Gederson Luiz Buzzello ◽  
Michelangelo Muzell Trezzi ◽  
José Abramo Marchese ◽  
Elouize Xavier ◽  
Edemir Miotto Junior ◽  
...  

Soybean genotypes grown in sub-tropical climate may exhibit lodging. The plant lodging is influenced by soil type and fertility level, sowing date, latitude and altitude of the location, plant population and conditions of crop development. Plant regulators and herbicides are able to avoid or reduce plant lodging. This study aimed to verify the effects of the growth regulators TIBA and daminozide on vegetative growth and yield of soybean cultivar CD 214 RR. The experiment was carried out at a field in randomized block design with four replications in a factorial scheme. The A factor was represented by the combination of regulators TIBA and daminozide and its concentrations, and the Factor B was seven times of evaluation of injury and plant height or eight times of evaluation of lodging. In the range of doses used, the application of daminozide resulted in greater injury to soybean plants than TIBA. The smaller plant height was achieved by the application of 6 g ha-1 of TIBA and 1200 g ha-¹ of daminozide. Treatments with daminozide (100 g ha-¹) and TIBA (10 g ha-1) stood out due to the reduced lodging of soybean plants. Grain weight increased linearly when the levels of TIBA increased. There was a negative correlation between lodging and grain yield and a positive correlation between plant height and lodging. There was also a negative correlation between injury caused by the application of plant regulators and lodging.


Author(s):  
Jamal Nasar ◽  
Ashfaq Alam ◽  
Muhammad Zubair Khan ◽  
Bilal Ahmed

The utilization of organic fertilizers in farmlands to enhance crop productivity and soil health is increasing globally. Compost and charcoal obtained from the decomposition and slow pyrolysis (respectively) of plants and animals wastes. Here we evaluated the effect of charcoal and compost on the growth and yield of wheat (Triticum aestivum L.) at Agriculture Research Farm Peshawar, Pakistan during 2014-2015. Results showed that the application of compost and charcoal as sole or in combination significantly affected the yield and yield attributes of wheat. Data divulged that the maximum numbers of plant tillers (337 m-2), number of productive tillers per plant (313 m-2), number of grains per spike (56 spike-1) and 1000 seeds weight (50.2 g) were produced by the combined application of compost and charcoal at 5+5 Mg ha-1 while plant height (95.3 cm) was enhanced with the application of charcoal at 10 Mg ha-1. The gain (4106.7 kg ha-1) and biological yield (8473.3 kg ha-1) was significantly increased with the compost application at the rate of 10 Mg ha-1. These results proved that charcoal and compost can be used as organic fertilizers for boosting growth and yield performance of wheat under the agro-climatic conditions of Peshawar, Pakistan.


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