scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Daun Pare (Momordica charantia L.) Terhadap Bakteri Staphylococcus aureus ATCC 25923

2021 ◽  
Vol 1 ◽  
pp. 1598-1604
Author(s):  
Kholila Rizqiana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Momordica Charantia ◽  
Diffusion Method ◽  
Bitter Melon ◽  
Disc Diffusion ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Chemical Content ◽  
Inhibitory Power

AbstractBitter gourd as a traditional medicine has a chemical content in its leaves that are useful as antimicrobials and as antioxidants. According to research by Tessa Undap, 2017 shows that bitter melon leaves have an inhibitory power against the growth of Staphylococcus aureus bacteria by inhibiting protein synthesis. The purpose of this study was to determine the antibacterial activity of bitter melon leaf extract (Momordica charantia L.). The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. The antibacterial results showed that the 9% extract concentration had the highest inhibitory power.Keywords: Antibacterial, Pare Leaf Extract, Granules, Staphylococcus aureus. AbstrakTanaman pare sebagai obat tradisional memiliki kandungan kimia dalam daunnya yang bermanfaat sebagai antimikroba dan sebagai antioksidan. Menurut penelitian oleh Tessa Undap, 2017 menunjukkan bahwa daun pare mempunyai daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dengan cara menghambat sintesis protein. Adanya kandungan flavonoid dalam daun pare dapat menghambat pertumbuhan bakteri Staphylococcus aureus yang dapat mengkoagulasi protein pada sel bakteri. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri yang terkandung dalam ekstrak daun pare (Momordica charantia L.). Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Hasil antibakteri yaitu menunjukkan bahwa konsentrasi ekstrak 9% mempunyai daya hambat paling tinggi.Kata kunci: Antibakteri, Ekstrak Daun Pare, Granul, Staphylococcus aureus.

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals Isolasi Bakteri Asam Laktat (BAL) dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) dan Kemampuannya Dalam Menghambat Staphylococcus aureus dan Shigella sp.

2020 ◽  
Vol 2 (1) ◽  
pp. 23-31
Author(s):  
Alpina Bukhori ◽  
Sartini Sartini ◽  
Rahmiati Rahmiati
Keyword(s):  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Digestive Tract ◽  
Oreochromis Niloticus ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Isolation And Characterization ◽  
Inhibitory Power

Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.

scholarly journals Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus

2007 ◽  
Vol 56 (7) ◽  
pp. 937-939 ◽  
Author(s):  
Naira Elane Moreira de Oliveira ◽  
Ana Paula Couto Marques Cardozo ◽  
Elizabeth de Andrade Marques ◽  
Kátia Regina Netto dos Santos ◽  
Marcia Giambiagi deMarval
Keyword(s):  
Staphylococcus Aureus ◽  
High Resistance ◽  
The Other ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Resistant Strains ◽  
Mupirocin Resistance ◽  
High Level

Meticillin-resistant Staphylococcus aureus isolates were classified into three mupirocin susceptibility groups by the disc diffusion method using 5 and 200 μg mupirocin discs. The zone diameter observed for a 5 μg disc distinguished MupS from the resistant strains (either MupRL or MupRH). On the other hand, a 200 μg disc distinguished the high-resistance MupRH strains from the other two (MupS or MupRL). Thus, the concomitant use of 5 and 200 μg mupirocin discs allowed the clear distinction among the three mupirocin susceptibility groups, MupS, MupRL or MupRH.

scholarly journals Screening of the antimicrobial activity of marine sponge extracts from Martinique

2021 ◽  
Author(s):  
Julie Piron ◽  
Jessica Pastour ◽  
Niklas Tysklind ◽  
Juliette Smith-Ravin ◽  
Fabienne Priam
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Marine Sponges ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Staphylococcus Saprophyticus

AbstractMarine sponges are known for their antimicrobial, antifungal, and cytotoxic activity. In this study, the activity of aqueous and ethanoic extracts of 3 sponges from Martinique were tested on 5 bacterial strains: Bascillus cereus (CIP 783), Echerichia coli (CIP 54127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125). The antimicrobial activity of Agelas clathrodes, Desmapsamma anchorata, and Verongula rigida, was demonstrated using the disc diffusion method and by determining the minimum inhibitory concentration and the minimum bactericidal concentration. The ethanoic extract of Agelas clathrodes had an inhibitory activity specifically on Staphylococcus aureus and Staphylococcus saprophyticus. No activity was observed for the other extracts. Further chemical analyses will be carried out in order to identify the active molecules of these sponges.

scholarly journals Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

2020 ◽  
pp. 48-57
Author(s):  
Ebrahim Sande ◽  
Danstone Lilechi Baraza ◽  
Selline Ooko ◽  
Peter Kuloba Nyongesa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Ganoderma Lucidum ◽  
Methicillin Resistance ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

scholarly journals Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

2021 ◽  
Vol 7 (1) ◽  
pp. 10-21
Author(s):  
Ismiyarto Ismiyarto ◽  
Niken Windi Saputri ◽  
Liswinda Zafirah Rahmatia ◽  
Purbowatiningrum Ria Sarjono ◽  
Ngadiwiyana Ngadiwiyana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Carboxymethyl Chitosan ◽  
Diffusion Method ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Chitosan Schiff Base

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain  Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

scholarly journals PERFORMANCE OF CHROM AGAR MEDIUM AND CONVENTIONAL METHODS FOR DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS.

2016 ◽  
Vol 9 (6) ◽  
pp. 136
Author(s):  
Nilima R Patil
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Care Hospital ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Methicillin Resistant ◽  
Conventional Methods

Background:- Methicillin resistant Staphylococcus aureus (MRSA) are responsible for hospital and community acquired infections. There are many laboratory methods for detection of MRSA. Chromogenic media have been used for the last few years for the quick detection of MRSA. Objective:- Aim of this study was to compare the performance of   conventional methods and chromogenic media for the detection of MRSA in a tertiary care hospital. Material and method: - 200 consecutive isolates of S. aureus confirmed by conventional methods, collected in a tertiary care hospital were used for this study. Cefoxitin and oxacillin disc diffusion test used as conventional methods and Chromogenic media i.e. oxacillin resistant screen agar base (ORSAB) was used for detection of methicillin resistant Staphylococcus aureus. All confirmed MRSA were checked by gold standard mecA base PCR method. Result: - Out of 200 isolates of Staphylococcus aureus, 50,52 and 47 strains were MRSA by Cefoxitin disc diffusion method, oxacillin disc diffusion method and oxacillin resistant screen agar base (ORSAB)  method respectively. Specificity was 100%, 98.66%, 98.66% by Cefoxitin disc diffusion, oxacillin disc diffusion and ORSAB method respectively. Conclusion: - In conclusion, cefoxitin disc diffusion was the best for the phenotypic detection of MRSA because their sensitivity and specificity were better than oxacillin and ORSAB.

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