Assesment Of Antioxidant Activity Test Of KERSEN Leaf (Muntingia calabura L.) And Epiphyte with DPPH (2.2-Diphenyl-1-Picrylhidrazyl)

2020 ◽  
Vol 1 (2) ◽  
pp. 60-66
Author(s):  
Nurlutfiyyah Aini ◽  
Fatmawati ◽  
Nita Parisa
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Linear Regression Analysis ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Human Needs ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Free Radical Activity ◽  
Muntingia Calabura

Antioxidant is very important to give protection against free radical activity and highlyreactive molecules that could lead in slowing the progression of degenerative disease.In case of degenerative disease, internal antioxidant cannot neutralize the increasingconcentration of free radical. Because of that, human needs external antioxidant.Kersen (Muntingia calabura L.) is a plant that is known for its antioxidant content.Plants containing antioxidant experience is kersen (Muntingia calabura L.). Researchstudy to determine the antioxidant activity of Kersen plant and knows the differenceof antioxidant activity, based on the process of extract and infusion. Research wasdone by experimental study which was oriented in testing antioxidant activity in(Morinda citrifolia L.) extract and infusion. Extraction was done by using 96% ethanolas solvent, meanwhile infusion was made by using aquadest. Extract and infusionwas divided into group of concentration and antioxidant activity was tested byDPPH(2,2-Diphenyl-1-Picrylhidrazyl) method by measuring the absorbance usingspectrophotometer at 520 nm wavelength. Percentage of DPPH inhibition and IC50then analyzed using linear regression analysis. Ethanolic extract of kersen leaf andepiphyte had IC50 value of 113,801 ppm and 98,7802 ppm, respectively. Kersen leafinfusion showed 191,7624 ppm IC50 values, besides its epiphyte had 131,6750 ppm.Antioxidant activity of Muntingia calabura L. in the order from kersen leaf anepiphyte, and epiphyte extract has a higher antioxidant content than others.

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

scholarly journals Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

2021 ◽  
Vol 5 (4) ◽  
pp. 365-368
Author(s):  
Muthmainah Tuldjanah ◽  
Niluh Puspita Dewi ◽  
Dian Rahmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Natural Antioxidants ◽  
Morinda Citrifolia ◽  
Absorption Method ◽  
Immersion Method ◽  
Activity Test ◽  
Ic50 Values

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)

2016 ◽  
Vol 3 (2) ◽  
pp. 159-163
Author(s):  
Arif Rahman ◽  
Abd Malik ◽  
Aktsar Roskiana Ahmad
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Free Radical Scavengers ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dried Fruit ◽  
Ethanol Extracts

Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.

scholarly journals Comparison of Antioxidant Activity of Ethanolic, Methanolic, n-Hexan, and Aqueous Extract of Parkia speciosa Peel based on Half -Maximal Inhibitory Concentration Through Free Radical Inhibition

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Fafa Nurdyansyah ◽  
Dyah Ayu Widyastuti
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Water Extract ◽  
Bark Extract ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Rotary Vacuum ◽  
Potential Content

The objectives of this study was to determine the half maximum inhibitory concentration (IC50) from four types of Parkia speciosa peel extracts (ethanol, methanol, n-hexane, and aqueous)  through DPPH free radical inhibition. First Parkia’s peel extract made by drying the Parkia’s peel that has been sorted, then crushed and mashed with a blender. Parkia’s powder then macerated for 3 replication using each type of solvent and then solvent evaporation was carried out using a rotary vacuum evaporator. The evaporated extract produced then tested for antioxidant activity using the IC50 method and phytochemical screening was performed to analyze the potential content of functional compounds. The results showed that all types of solvents dissolve alkaloid compounds (except water extract), flavonoids, saponins, tannins, and phenols. IC50 values produced from the four types of petai bark extract using methanol, ethanol, water, and n-hexane solvents sequentially were 76.92; 111; 136; and 201 ppm. Methanol extract had the lowest IC50 value of 76.92 ppm which resulted that the methanol extract of petai skin had a strong (active) antioxidant strength compared to others.Keywords: Antioxidant, Exctract, Parkia’s peel, IC50

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN KRIM EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 349
Author(s):  
Eklesia Pogaga ◽  
Paulina V. Y. Yamlean ◽  
Julianri S. Lebang
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Traditional Medicine ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Mulberry Leaves ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Ethanol Extracts

ABSTRACTMulberry is one of the plants growing in Indonesian and widely used as traditional medicine. The active compounds found in Mulberry leaves are known have an antioxidant activity. The aim of this study is to formulate cream preparations from ethanol extracts of Mulberry leaves and to determine its antioxidant activities. Mulberry leaves was macerated using ethanol 96%. Antioxidant activity of mulberry leaves cream then evaluated against DPPH as free radical and measured at 517 nm wavelength using a UV-Vis spectrophotometer. The results showed that the cream of ethanol extract from Mulberry leaves had a very strong antioxidant activity with IC50 values in a row that is 1.7831 ppm, 0.8215 ppm and 0.7668 ppm. This can be compared with a Vitamin C solution, which has a very strong antioxidant activity with IC50 value was 1.1113 ppm. This study concludes that the cream from Mulberry leaves ethanol extract has a very strong antioxidant activity.Keywords: Mulberry leaves, Cream, Antioxidant, DPPHABSTRAK Murbei merupakan salah satu tanaman yang tumbuh di Indonesia dan  banyak digunakan dalam pengobatan secara tradisional. Kandungan senyawa aktif yang terdapat pada daun Murbei dikenal mempunyai peranan sebagai antioksidan. Tujuan dari penelitian ini ialah untuk membuat sediaan krim dari ekstrak etanol daun Murbei dan untuk menguji apakah adanya aktivitas antioksidan. Daun murbei diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Sediaan krim diuji terhadap DPPH sebagai radikal bebas dan diukur pada panjang gelombang 517 nm  dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa krim ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat  dengan  nilai IC50 berturut-turut yaitu 1,7831 ppm, 0,8215 ppm dan 0,7668 ppm. Hal ini dapat dibandingkan dengan larutan Vitamin C yang mempunyai aktivitas antioksidan yang sangat kuat, yaitu 1.1113 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim dari ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Daun Murbei, Krim, Antioksidan, DPPH

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

Synthesis and Structure−Activity Relationship Studies of N-monosubstituted Aroylthioureas as Urease Inhibitors

2020 ◽  
Vol 16 ◽  
Author(s):  
Wei-Wei Ni ◽  
Hai-Lian Fang ◽  
Ya-Xi Ye ◽  
Wei-Yi Li ◽  
Li Liu ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Mammalian Cells ◽  
Intact Cell ◽  
Linear Regression Analysis ◽  
Positive Control ◽  
Acetohydroxamic Acid ◽  
Urease Inhibitors ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Low Cytotoxicity

Background: Thiourea is a classical urease inhibitor usually as a positive control, and many N,N`-disubstituted thioureas have been determined as urease inhibitors. However, due to steric hindrance, N,N`-disubstituted thiourea motif could not bind urease as thiourea. On the contrary, N-monosubstituted thioureas with a tiny thiourea motif could theoretically bind into the active pocket as thiourea. Objective: A series of N-monosubstituted aroylthioureas were designed and synthesized for evaluation as urease inhibitors. Methods: Urease inhibition was determined by the indophenol method and IC50 values were calculated using computerized linear regression analysis of quantal log dose-probit functions. The kinetic parameters were estimated viasurface plasmon resonance (SPR) and by nonlinear regression analysis based on the mixed type inhibition model derived from Michaelis-Menten kinetics. Results: Compounds b2, b11and b19 reversibly inhibited urease with a mixed mechanism, and showed excellent potency against both cell-free urease and urease in intact cell, with IC50 values being 90-to 450-fold and 5-to 50-fold lower than the positive control acetohydroxamic acid, respectively. The most potent compound b11 showed IC50 value of 0.060 ±0.004μM against cell-free urease, which bound to urea binding site with a very low KDvalue (0.420±0.003nM) and a very long residence time (6.7 min). Compound b11was also demonstrated having very low cytotoxicity to mammalian cells. Conclusion: These results revealed that N-monosubstituted aroylthioureas clearly bind the active site of urease as expected, and represent a new class of urease inhibitors for the development of potential therapeutics against infections caused by ure-ase-containing pathogens.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Antioxidant Activity Sonneratia caseolaris Leaves Extract at Different Maturity Stages

2020 ◽  
Vol 8 (3) ◽  
pp. 130
Author(s):  
Winarti Winarti ◽  
Boedi Setya Rahardja ◽  
Sudarno Sudarno
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Heart Attack ◽  
Total Content ◽  
Reactive Component ◽  
Mature Leaves ◽  
Maturity Stages ◽  
Sonneratia Caseolaris ◽  
Young Leaves ◽  
Ic50 Values

Free radical is a reactive component because it has one or more electrons. Free radical is very dangerous for our body because it can cause degenerative diseases, such as cancer, heart attack, and aging. Cancer and heart attack are one of the most killer disease. All we can do to decrease the free radical effect is consume antioxidant. S. caseolaris leaves has antioxidant activity, but it has known yet which stage of maturity is the best one for produce the highest antioxidant activity. This purpose of this research is knowing the influence between maturity and antioxidant activity at different maturity stages. This method of this research is experimental with Completely Randomized Design. The treatment in this research is vitamin C, fresh leaves in ethanol 96% extract, young leaves in ethanol 96% extract, and mature leaves in ethanol 96% extract. The main parameter of this research is IC50 values and the secondary parameters are total content of phytochemical in every extract. Data analysis using descriptive method with 4 treatments and five replications. The result of this research show that the maturity has influence in antioxidant activity. Inhibitory concentration 50 (IC50) values of shoot leaves extract, young leaves extract and mature leaves extract is 12.0013 ppm, 13.9915 ppm and 14.6613 ppm. All of them are called antioxidant which has very strong activities. The highest phytochemical compound is found on shoot leaves extract with ethanol 96% solvent.

scholarly journals Evaluation antioxidant activity and corrosion inhibition of C38 in Hydrochloric acid medium by dried lemon peels of Kenitra Marrakech cities in Morocco and Taiz town in Yemen: A Comparative study

2021 ◽  
Vol 119 ◽  
pp. 04001
Author(s):  
Khaled Abdu ◽  
Rahma Erahioui ◽  
Khadija Khedid ◽  
Hefdhal deen ◽  
Maha Elhawary ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Corrosion Inhibition ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Fatty Tissue ◽  
Scavenging Activity ◽  
Ic50 Values

This paper presents a comparative study about the evaluation of antioxidant activity and corrosion inhibition in Kenitra, Marrakesh and Taiz. An interesting topic, indeed polyphenols can improve or help to reduce oxidative stress in the treatment of digestive problems, weight management difficulties, diabetes, hypertension, arteriosclerosis and damage fatty tissue. Therefore, the authors deal with the measurement of polyphenol content and evaluation of the antioxidant activity of lemon peels in Kenitra , Marrakesh and Taiz.The authors performs empirical analyzes on lemon peels. The total polyphenol contents of the ethanolic extract of lemon peels were measured. It was to be 30. 23, 26. 346 and 20.961 mg/CE/g in Kenitra, Marrakech, and Taiz, respectively. Moreover, the DPPH radical scavenging activity of ethanolic extract of dried lemon peels was higher than 200μg/ml concentration. They were 73.47%, 47.36%, and 32.09in Kenitra, Marrakech, and Taiz, respectively. Also, the IC50 values of ethanolic extracts calculated from the percentage inhibitions at the same concentration. Inhibition (IC50) which obtained in Kenitra was 123.089 μg/ml. It was lower compared to the ethanol extract of Marrakech and Taiz that were 197.418, 276.750μg/mL, respectively. Therefore, the extract which is containing a high amount of phenolic is showed high radical scavenging activity. In addition, the maximum inhibition efficiencies for 2 mL L-1 of the inhibitor at 298 k were 98, 12% and 84, 85 % in Kenitra and Taiz, respectively. These values obtained through polarization curve measurements.

Sign in / Sign up

Export Citation Format

Share Document