POTENSI EKSTRAK DAUN TANAMAN KARAMUNTING (Melastoma malabathricum L.) DI DAERAH KALIMANTAN SEBAGAI ANTIBAKTERI Staphylococcus aureus

2018 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Rakhmadhan Niah ◽  
Riki Nirwan Baharsyah
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infection ◽  
In Vitro Studies ◽  
Plant Leaf ◽  
Extract Concentration ◽  
Melastoma Malabathricum ◽  
Agar Method ◽  
Microbiology Research ◽  
Growth Of Bacteria

The year 2008 recorded the death toll reached 16,769 soul due to infection. Infection is one of the diseases caused by bacteria or parasites. Staphylococcus aureus is one of the bacteria causes infection of the skin that is often the case in Indonesia. Staphylococcus aureus bacterial infection can be treated naturally, one of the plants that can be utilized is plant karamunting. Plant leaf karamunting (Melastoma malabathricum l.) contain flavonoids and alkaloids that have activity as antibacterial. The purpose of this research is to know the power of drag karamunting folium of extract against the growth of Staphylococcus aureus. In vitro studies were conducted with diffusion agar method. The extraction of leaf maceration method done with karamunting solvent ethanol 96%. Concentration of karamunting folium of extract used are: 25%, 50%, 75%, and 100%. Microbiology research results indicate that karamunting folium of extract have drag against the growth of bacteria Staphylococcus aureus with extract concentration 50% yield of 5.34 mm; the concentration of extract 75% of 9.40 mm; the concentration of extract 100% of 12.43 mm.

scholarly journals Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus aurantifolia s.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro

2013 ◽  
Vol 2 (1) ◽  
pp. 05 ◽  
Author(s):  
Abdul Razak ◽  
Aziz Djamal ◽  
Gusti Revilla
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Contact Time ◽  
Experimental Methods ◽  
Control Group ◽  
Control Group Design ◽  
Citrus Aurantifolia ◽  
Group Design ◽  
Growth Of Bacteria

AbstrakJeruk Nipis (Citrus aurantifolia S.) merupakan salah satu tanaman obat keluarga yang banyak terdapat ditengah masyarkat dan banyak digunakan sebagai ramuan tradisional. Bagian yang sering digunakan adalah air perasannya, dengan salah satu manfaat dapat digunakan untuk menghilangkan jerawat serta penyembuhan luka agar tidak terjadi abses. Jerawat dan abses pada luka merupakan salah satu infeksi yang disebabkan oleh bakteri Staphylococcus aureus.Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia S.) terhadap pertumbuhan bakteri Staphylococcus aureus secara invitro. Penelitian dilakukan dengan metoda eksperimental laboratorium dengan desain postest only control group design yang dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas.Hasil penelitian menunjukan bahwa air perasan buah jeruk nipis memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dengan berbagai konsentrasi yaitu 25%, 50%, 75%, dan 100% dan terdapat pengaruh lama kontak terhadap pertumbuhan bakteri dimana bakteri tidak tumbuh seteleh kontak 5 menit pertama dan diikuti menit-menit berikutnya dengan air perasan buah jeruk nipis konsentrasi 100%. Jadi, semakin tinggi konsentrasi air perasan buah jeruk nipis dan semakin lama kontak dengan bakteri Staphylococcus aureus maka daya hambatnya semakin baik.Kata kunci: Uji Daya Hambat, Air Perasan Buah Jeruk Nipis, Staphylococcus aureus.Abstract Lime (Citrus aurantifolia S.) is kind of family’s herbal medicine, most using in the community is widely used as a traditional herb. The most common used part is the lime fruit squeeze with one of the function is used for removing acne and wound healing to prevent the form of abscess. Pimples and abscesses of the wound is one of the infections caused by the bacterium Staphylococcus aureus.The purpose of this study was to determine the inhibition of lime fruit (Citrus aurantifolia S.) squeeze towards the growth of the bacteria Staphylococcus aureus in vitro condition. The study was conducted with laboratory experimental methods to the design of control group design postest only performed at the Laboratory of Microbiology Faculty of Medicine, University of Andalas.The results showed that the lime fruit (Citrus aurantifolia S.) squeeze has the ability to inhibite the bacterial growth of Staphylococcus aureus with various concentrations of 25%, 50%, 75%, and 100% and there is the effect of contact time on the growth of bacteria which the bacteria do not grow after contact the first 5 minutes and the next minute followed by lime fruit squeeze with 100% concentration lime fruit squeeze. Thus, the higher the concentration of lime fruit squeeze and the longer the contact with the bacteria Staphylococcus aureus is the better towards.Keywords:Inhibition test, The Lime Fruit Squeeze, Staphylococcus Aureus.

Teicoplanin, vancomycin, rifampicin: in-vivo and in-vitro studies with Staphylococcus aureus

1987 ◽  
Vol 19 (5) ◽  
pp. 659-662 ◽  
Author(s):  
Holliday T. Carper ◽  
Gail W. Sullivan ◽  
Gerald L. Mandell
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Studies

scholarly journals The Effects of Turmeric Infusion and Turmeric Juice (Curcuma Domestica) on The Staphylococcus Aureus Growth in Vitro

2021 ◽  
Vol 2 (2) ◽  
pp. 54-60
Author(s):  
Renny Novi Puspitasari ◽  
Handayani Handayani ◽  
Ratna Sofaria
Keyword(s):  
Staphylococcus Aureus ◽  
Pearson Correlation ◽  
Food Poisoning ◽  
Average Growth ◽  
Phenol Derivatives ◽  
Group I ◽  
The One ◽  
Insect Bites ◽  
Growth Of Bacteria

Background: Turmeric is a plant that grows in tropical areas and functions as medicine. The chemical compounds contained in turmeric have a role as antioxidants, antimicrobials, anti-cancer, indigestion, smallpox, insect bites. The curcumin content in turmeric has antibacterial activity against various types of Gram negative, Gram positive, antiviral and anti-tumor bacteria. Essential oils can be used as antibacterial because they contain hydroxyl and carbonyl functional groups which are phenol derivatives. Flavonoids can interfere with cell wall formation with peptidoglycan transpeptidase activity which will break down cell walls and damage cell membranes so that important components such as proteins, nucleic acids, nucleotides will be lysis. Staphylococcus bacteria are normal flora on the skin, respiratory tract, and digestive tract of food in humans. These bacteria can cause disease when they reach 1,000,000 or 106 per gram, an amount sufficient to produce the toxin. S. aureus bacteria can cause various types of infections ranging from minor skin infections, food poisoning to systemic infections. The aim of our study was to analyze the effects of infusion and turmeric juice (Curcuma domestica val) on the growth of bacteria Staphylococcus aureus through invitro. Method: This study is a laboratory experimental study with the aim of analyzing turmeric infusion (Curcuma domestica val) and turmeric juice effect on the growth of staphylococcus aureus by invitro. This research was conducted in an integrated laboratory, FK UNUSA. The samples in this study were 4 replications per treatment. The independent variables in this study were turmeric infusion and turmeric juice (with a concentration of 10%, 30%, 50% while the dependent variable in this study was the growth of Staphylococcus aureus bacteria. Data analysis used was the one way ANOVA test and Pearson correlation to determine the effect of giving turmeric infusion and turmeric juice on the growth of bacteria staphylococcus aureus through invitro. Result: The average growth of bacteria in the turmeric infusion in group I (control), 10%, 30% and 50% of turmeric infusion was 4.89 ± 0.4425 log CFU / ml, 3.07 ± 0.61330 log CFU / ml, 2.99 ± 0.63986 log CFU. / ml and 3.02 ± 0.22650 log CFU / ml. The average growth of this bacteria in giving turmeric juice in group I (control), giving 10%, 30% and 50% turmeric infusion was 4.89 ± 0.04425 log CFU / ml, 4.40 ± 0.18355log CFU / ml, 3.10 ± 0.58926 log CFU / ml and 3.02 ± 0.38206 log CFU / ml. Conclusion: In this study, found that there was an effect of giving turmeric infusion and turmeric juice (Curcuma domestica val) on Staphylococcus aureus growth and there was an effect of giving multilevel doses of turmeric infusion and turmeric juice (Curcuma domestica val) on Staphylococcus aureus growth through invitro.

Compatibility of Cephalosporins and Aminogl Ycosides in Peritoneal Dialysis Fluid

1983 ◽  
Vol 3 (3) ◽  
pp. 128-129 ◽  
Author(s):  
Carol Loeppky ◽  
Eugene Tarka ◽  
E. Dale Everett
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Peritoneal Dialysis ◽  
In Vitro Studies ◽  
Dialysis Fluid ◽  
Peritoneal Dialysis Fluid ◽  
The Individual ◽  
And Staphylococcus Aureus

Often dialysis -associated peritonitis is treated before the results of cultures are known with a cephalosporin and an aminoglycoside in combination. Because there may be antagonism between the individual drugs in such combinations, we have investigated this possibility through the use of timed, killing curves in dialysate effluent. We tested various cephalosporins and aminoglycosides alone and in combination at concentrations usually instilled into the peritoneum and determined their activity against one strain each of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The results of these in-vitro studies show no evidence of antagonism but rather suggest an additive effect as evidenced by more rapid killing.

scholarly journals Plectasin Shows Intracellular Activity against Staphylococcus aureus in Human THP-1 Monocytes and in a Mouse Peritonitis Model

2009 ◽  
Vol 53 (11) ◽  
pp. 4801-4808 ◽  
Author(s):  
Karoline Sidelmann Brinch ◽  
Anne Sandberg ◽  
Pierre Baudoux ◽  
Françoise Van Bambeke ◽  
Paul M. Tulkens ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Animal Studies ◽  
Kinetic Studies ◽  
Response To Therapy ◽  
In Vitro Studies ◽  
In Vivo Model ◽  
Intracellular Activity ◽  
Peritonitis Model

ABSTRACT Antimicrobial therapy of infections with Staphylococcus aureus can pose a challenge due to slow response to therapy and recurrence of infection. These treatment difficulties can partly be explained by intracellular survival of staphylococci, which is why the intracellular activity of antistaphylococcal compounds has received increased attention within recent years. The intracellular activity of plectasin, an antimicrobial peptide, against S. aureus was determined both in vitro and in vivo. In vitro studies using THP-1 monocytes showed that some intracellular antibacterial activity of plectasin was maintained (maximal relative efficacy [E max], 1.0- to 1.3-log reduction in CFU) even though efficacy was inferior to that of extracellular killing (E max, >4.5-log CFU reduction). Animal studies included a novel use of the mouse peritonitis model, exploiting extra- and intracellular differentiation assays, and assessment of the correlations between activity and pharmacokinetic (PK) parameters. The intracellular activity of plectasin was in accordance with the in vitro studies, with an E max of a 1.1-log CFU reduction. The parameter most important for activity was fC peak/MIC, where fC peak is the free peak concentration. These findings stress the importance of performing studies of extra- and intracellular activity since these features cannot be predicted from traditional MIC and killing kinetic studies. Application of both the THP-1 and the mouse peritonitis models showed that the in vitro results were similar to findings in the in vivo model with respect to demonstration of intracellular activity. Therefore the in vitro model was a good screening model for intracellular activity. However, animal models should be applied if further information on activity, PK/pharmacodynamic parameters, and optimal dosing regimens is required.

scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL SURUHAN (Piperumia pellucida L.H.B Kunth) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN-VITRO

2015 ◽  
Vol 2 (1) ◽  
pp. 8-14
Author(s):  
Eldo Dandirwalu ◽  
Theopilus W Watuguly
Keyword(s):  
Staphylococcus Aureus ◽  
Science Education ◽  
Healthy Individual ◽  
Research Use ◽  
Extract Concentration ◽  
Staphyloccocus Aureus ◽  
Traditional Drug ◽  
Original Plant ◽  
Weak Resistance

Background: Staphyloccocus aureus represent normal mikroflora of human being. This bacterium usually there are at bronchi to the and husk. Existence of Staphyloccocus aurues at bronchi to the and husk at individual seldom cause disease, healthy individual usually only personating career. One of the original plant that is plant of suruhan (Piperumia pellucida L.H.B. Kunth) biggest and wide of in some area in Indonesia (Molluca) and also have potency to be developed as traditional drug, empirically have been used in medication of abscess (abses) by society. Methods: This Research use eksperimental in Laboratory with research device is Complete Random Device (Factorial RAL) Pattern from two factor and 3 restating, which in doing/conducting in Elementary Laboratory of Biology Faculty Teachership and Science Education of Pattimura University. Resulth: Result research of to extract etanol plant suruhan (Piperumia pelludica L.H.B Kunth) to growth of bacterium of Staphylococcus aureus with various concentration that is 25%, 50% and 75% and there is influence contact to growth of bacterium where at concentration 25% is equal to 5 mm donot give respon pursue, 50% is equal to 10 mm with weak resistance respon and 75% is equal to 16 with resistance respon. Conclusion: Extract concentration excelsior of etanol plant of suruhan (Piperumia pelludica L.H.B Kunth) and old progressively of contact with bacterium of Staphylococcus aureus hence resistivity progressively goodness

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ASCIDIAN (Lissoclinum badium) DARI PERAIRAN PULAU MANTEHAGE

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 897
Author(s):  
Falinry Woran ◽  
Defny S. Wewengkang ◽  
Meilani Jayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Methanol Fraction ◽  
Agar Method ◽  
Ethanol Extracts ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Growth Of Bacteria

ABSTRACTLissoclinum badium is a type of ascidian that contains bioactive compounds. This study aims to determine of presence of antibacterial activity from extracts and fractions of Lissoclinum badium collected from Mantehage Island Manado against Escherichia coli and Staphylococcus aureus bacteria. Samples were extracted by maceration method using 95% ethanol solvent and fractionated using solvents of chloroform, n-hexane and methanol. Antibacterial activity was carried out by the disk diffusion agar method. The results showed that the ethanol extracts an methanol fraction had activity to inhibit the growth of Escherichia coli bacteria with strong category. Meanwhile, against the Staphylococcus aureus the ethanol extracts, chloroform and n-hexane fractions had ability to inhibit the growth of bacteria with weak category.. Keywords: Antibacterial activity, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.  ABSTRAKLissoclinum badium merupakan salah satu jenis tunikata yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi  Lissoclinum badium yang diperoleh dari Pulau Mantehage Manado terhadap Escherichia coli dan Staphylococcus aureus. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol 95% dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antibakteri dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi metanol memiliki aktivitas untuk menghambat bakteri Escherichia coli dan Staphylococcus aureus dengan daya hambat kuat. Sedangkan untuk fraksi kloroform dan fraksi n-heksan memiliki aktivitas untuk menghambat bakteri Staphylococcus aureus saja dengan daya hambat sedang. Kata kunci: Aktivitas antibakteri, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.

scholarly journals Caerin 1.1 and 1.9 peptides from Australian tree frog inhibit antibiotic-resistant bacteria growth in a murine skin infection model

2021 ◽  
Author(s):  
Shu Chen ◽  
Pingping Zhang ◽  
Liyin Xiao ◽  
Ying Liu ◽  
Kuihai Wu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infection ◽  
Bacterial Resistance ◽  
Resistant Bacteria ◽  
Infection Model ◽  
Temperature Sensitive ◽  
Tree Frog ◽  
Bacteria Growth ◽  
Wide Range

AbstractHost-defence caerin 1.9 peptide was originally isolated from skin secretion of Australian tree frog, and inhibits the growth of a wide range of bacteria in vitro. In this study, we demonstrated that caerin 1.9 shows high bioactivity against several bacteria strains, such as Staphylococcus aureus, Acinetobacter Baumannii, methicillin-resistant Staphylococcus aureus (MRSA), and Streptococcus hemolyiicus in vitro. Importantly, unlike antibiotic Tazocin, caerin 1.9 does not induce bacterial resistance after 30 rounds of in vitro culture. Moreover, caerin 1.1, another peptide of caerin family, has additive antibacterial effect when used together with caerin 1.9. Furthermore, caerin 1.1 and 1.9 prepared in the form of a temperature sensitive gel inhibit MRSA growth in skin bacterial infection model of two murine strains. These results indicate that caerin 1.1 and 1.9 may have the advantage than conventional antibiotics against bacterial infection of skin.

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