UJI EFEKTIVITAS ANTIBAKTERI ESKTRAK ETIL ASETAT BAWANG DAYAK (Eleutherine palmifolia Merr) TERHADAP STAPHYLOCOCCUS AUREUS DENGAN METODE SUMURAN

2020 ◽  
Vol 5 (2) ◽  
pp. 174
Author(s):  
Fitriyanti Fitriyanti ◽  
Abdurrazaq Abdurrazaq ◽  
Muhammad Nazarudin
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Effective Concentration ◽  
Minimum Effective Concentration ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Dayak onion (Eleutherine palmifolia Merr.) is a multifunctional medicinal plant for various diseases, one of which is an antibacterial. Dayak Onions (E. palmifolia Merr.) contains an alkaloid, flavonoid, triterpenoid, and saponin compound which has antibacterial properties. This study aims to determine the effect of the ethyl acetate extract of Dayak Onion tubers inhibits the growth of Staphylococcus aureus bacteria. Dayak onion tuber was extracted using ethyl acetate solvent by the maceration method until thick extract was obtained. The concentration extract used were 30 mg/ml;15 mg/ml;7,5 mg/ml;3,75 mg/ml; 1,875 mg/ml; and 0,9375 mg/ml tested for antibacterial effectivity against Staphylococcus aureus using Mueller Hinton Agar (MHA) with the wells method. The result showed that the ethyl acetate of Dayak onion with MEC (Minimum effective concentration) 3,75 mg/ml obtained inhibition zone of 10,367 mm while at the highest concentration of 30 mg/ml had the best antibacterial effectiveness. The effective concentration of ethyl acetate onion of Dayak tuber which is 30 mg/ml obtained inhibition zone of 18,404 mm even in the moderate category.

scholarly journals ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.)

2017 ◽  
Vol 4 (2) ◽  
pp. 172
Author(s):  
Srikandi Srikandi ◽  
I.G.A. Manik Widhyastini
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Solvent Concentration ◽  
Garcinia Mangostana ◽  
A Value ◽  
Hexane Extracts

Antibacterial Extract of Mangosteen (Garcinia mangostana L.)Fruit Shell           The skin of the mangosteen fruit is extracted with n-hexane and ethyl acetate to determine the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27 853. Results showed that n-hexane extract gave inhibition zone larger than the ethyl acetate extract on all concentrations . Extract n-hexane has a value of MIC against S. aureus ATCC bacterial test 25923 62.5 mg / ml while the ethyl acetate extract of 125 mg / ml . N- hexane extracts had MIC values of the test bacteria P.aeuroginosa ATCC 27 853 was 125 mg / ml , and while the ethyl acetate extract had a MIC value of 500 mg / ml . Treatment of solvent, concentration and interaction between the solvent and concentration significantly affected the test bacteria ATCC 25923 S. aureus at the level of 5 %, the highest interaction N-Hexane solvent with a concentration of 15,625 mg / ml and was not significantly different interactions with the concentration of 31.25 mg/ml and 125 mg/ml. Treatment solvent and concentration significantly while the interaction between the solvent and the concentration has no effect on the test bacteria P.aeuroginosa ATCC 27 853 at 5% level .Keywords: Garcinia mangostana L., Staphylococcus aureus and Pseudomonas aeruginosa ABSTRAK           Kulit buah manggis diekstrak dengan n-heksan dan etil asetat   untuk mengetahui Konsentrasi Hambat Minimum (KHTM) terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeruginosa ATCC 27853. Hasil penelitian menunjukkan bahwa ekstrak n-heksana  memberikan zona hambatan lebih besar dibandingkan dengan ekstrak etil asetat pada semua konsentrasi. Ekstrak n-heksana  memiliki nilai kadar hambat minimal (KHM) terhadap bakteri uji S. aureus ATCC 25923  62,5 mg/ml sedangkan ekstrak etil asetat 125 mg/ml.   Ekstrak n-heksana memiliki nilai KHM   terhadap bakteri uji P.aeuroginosa  ATCC 27853 adalah 125 mg/ml dan  sedangkan ekstrak etil asetat memiliki nilai KHM  500 mg/ml. Perlakuan pelarut, konsentrasi dan interaksi antara pelarut dan konsentrasi  berpengaruh nyata terhadap bakteri uji S. aureus ATCC 25923 pada taraf 5%, interaksi tertinggi yaitu pelarut N-Heksan dengan konsentrasi 15,625mg/ml dan interaksi ini tidak berbeda nyata dengan konsentrasi 31,25 mg/ml dan 125 mg/ml. Perlakuan pelarut dan konsentrasi  berpengaruh nyata sedangkan interaksi antara pelarut dan konsentrasi  tidak berpengaruh terhadap bakteri uji P.aeuroginosa  ATCC 27853 pada taraf 5%. Kata kunci:  Garcinia mangostana L., Staphylococcus aureus dan Pseudomonas aeruginosa

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Kimia ◽  
2020 ◽  
pp. 56
Author(s):  
N. M. Puspawati ◽  
N. L. P. F. Widiari ◽  
I M. Sukadana
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Spectra Analysis ◽  
Minimum Concentration ◽  
Butanol Extract ◽  
Leaf Powder ◽  
Inhibition Zones

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly.    Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.

scholarly journals Poguntano Herba Extract Immunostimulant Activities (Picriafel-terraeLour). in Immunosupression Rats Infected by Staphylococcus aureus Against Total Leukocytes and Differential Leukocytes

2021 ◽  
Vol 4 (1) ◽  
pp. 39-46
Author(s):  
Anggitha Ningtias ◽  
Rosidah ◽  
Yuandani
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Traditional Medicine ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Flavonoid Compounds ◽  
North Sumatra

Poguntano (Picriafel-terraeLour.) is a medicinal plant in North Sumatra that has been used in traditional medicine to treat degenerative diseases.Poguntano extract contains flavonoid compounds that have the potential to be developed into immunomodulators. This study showed the immunostimulant activity of poguntano herb n-hexane extract (PHNHE), poguntano herb ethyl acetate extract (PHEAE) and poguntano herb ethanol extract (PHEE) in immunosuppressed rats infected with Staphylococcus aureus. The evidence of poguntano herb as an immunomodulator was carried out by testing total leukocytes and differential leukocytes. Poguntano herb n-hexane extract (PHNHE) can not increase the total and differential leukocytes; Poguntano herb ethyl acetate extract (PHEAE) and poguntano herb ethanol extract (PHEE) can increase the total and differential leukocytes in immunosuppressed rats induced by Staphylococcus aureus.

scholarly journals Antibacterial Activity of Grape Seeds Extracts (Vitis vinifera L.) Against Streptococcus mutans ATCC 31987

2021 ◽  
Vol 1 (1) ◽  
pp. 50-58
Author(s):  
Fathin Hamida ◽  
Vilya Syafriana ◽  
Carla Febriayu Ramadhani ◽  
Elsa Vera Nanda
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Grape Seeds ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

ABSTRAK Streptococcus mutans merupakan salah satu bakteri penyebab karies gigi. Penggunaan antibiotic merupakan terapi infeksi yang umum dilakukan untuk menghambat pertumbuhan bakteri. Biji anggur diketahui mengandung senyawa polifenol yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas ektrak biji anggur terhadap Streptococcus mutans. Bahan uji yang digunakan adalah buah anggur yang diambil bijinya. Buah anggur diperoleh dari Pasar Induk, Kramat Jati, Jakarta Timur. Ekstrak biji anggur diperoleh dengan metode maserasi menggunakan pelarut etil asetat dan etanol 70%. Uji aktivitas antibakteri dilakukan menggunakan metode difusi cakram pada media Mueller Hinton Agar dengan konsentrasi 5%, 10%, 20%, dan 40%. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% biji anggur memiliki aktivitas antibakteri pada konsentrasi 5%, 10%, 20%, dan 40% dengan nilai hambatan secara berurutan sebesar 8,46 mm; 8,91 mm; 9,89 mm; dan 11,04 mm. Hasil pada ekstrak etil asetat juga menunjukkan ada aktivitas penghambatan pada konsentrasi 5%, 10%, 20%, dan 40% dengan nilai hambatan secara berurutan sebesar 7,72 mm; 8,50 mm; 9,64 mm; dan 10,51 mm. Ekstrak etanol 70% biji anggur memiliki daya hambat yang lebih besar dibandingkan dengan ekstrak etil asetat. Hasil ini dapat menjadi acuan untuk pengembangan potensi biji anggur sebagai bahan obat atau kosmedik (kosmetik-medik) penanganan karies gigi.   ABSTRACT Streptococcus mutans is one of the bacteria that cause dental caries. The antibiotics usually used for the therapy of this infection to inhibit bacterial growth. Polyphenol, which recommended as an antibacterial agent, is common secondary metabolites in grape seeds. The purpose of this study is to know the activity of grape seed extract against Streptococcus mutans. The grape seeds were obtained from fresh fruits which bought from Pasar Induk, Kramat Jati, East Jakarta. The grape seed extract was obtained by the maceration method using ethyl acetate and 70% ethanol. The antibacterial activity test was carried out using the disk diffusion method on Mueller Hinton Agar media with a concentration of 5%, 10%, 20%, and 40%. The results showed that 70% ethanol extract of grape seeds had antibacterial activity at concentrations of 5%, 10%, 20%, and 40% with a value of 8.46 mm; 8.91 mm; 9.89 mm; and 11.04 mm respectively. The results of ethyl acetate extract also showed inhibitory activity at concentrations of 5%, 10%, 20%, and 40% with values ​​of 7.72 mm; 8.50 mm; 9.64 mm; and 10.51 mm respectively. The inhibition of 70% ethanol extract of grape seed is greater than ethyl acetate extract. The results of the study can be used as reference for the potential development of grape seed as a medicinal or cosmedic cosmetic-medical) ingredient for treating dental caries.

Ocotea nutans (Nees) Mez: structural elucidation of C-hetorosides flavonoids and evaluation of their antioxidant and antibacterial properties from ethyl acetate extract

2021 ◽  
pp. 1-5
Author(s):  
Fernando Cesar Martins Betim ◽  
Camila Freitas de Oliveira ◽  
Katlin Suellen Rech ◽  
Angela Maria Souza ◽  
Obdulio Gomes Miguel ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Structural Elucidation

scholarly journals Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

2019 ◽  
Vol 10 (2) ◽  
pp. 94-98
Author(s):  
Adlis Santoni ◽  
Mai Efdi ◽  
Akmel Suhada
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Endemic Plant ◽  
Dpph Method ◽  
Hexane Extracts

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity  was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibition zone 10 mm at  concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

scholarly journals Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

2021 ◽  
Vol 11 (20) ◽  
pp. 9697
Author(s):  
Eduardo Padilla-Camberos ◽  
Omar Ricardo Torres-Gonzalez ◽  
Ivan Moises Sanchez-Hernandez ◽  
Nestor Emmanuel Diaz-Martinez ◽  
Oscar Rene Hernandez-Perez ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inflammatory Activity ◽  
Therapeutic Effects ◽  
Ear Edema ◽  
Croton Oil ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

Polysaccharide-Based Film of Cassia fistula and it's Antibacterial Activity

2014 ◽  
Vol 875-877 ◽  
pp. 87-90 ◽  
Author(s):  
Chutimon Satirapipathkul ◽  
Tanakan Chatdum
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Water Vapor Permeability ◽  
Inhibition Zone ◽  
Vapor Permeability ◽  
Glycerol Concentration ◽  
Bacterial Strains ◽  
Cassia Fistula

The film-forming potential of isolate of seed polysaccharide fromCassia fistulawas investigated. Increasing the glycerol concentration in the film increased elongation at break, film solubility and water vapor permeability but decreased tensile strength (TS). The film impregnated with the acetone extract ofAtractylodes lanceawas assessed for inhibition ofEscherichia coliandStaphylococcus aureus. The obtained results showed that the films exhibited antibacterial activity against both bacterial strains. Disc-diffusion assay revealed that the film resulted in a larger inhibition zone around the film onStaphylococcus aureusthan it did onEscherichia coliat the same extract concentrations (0.1 to 0.9 wt %). It can be seen that the film has satisfactory physical and antibacterial properties.

Rhodomyrtone, an antibotic from Rhodomyrtus tomentosa

2002 ◽  
Vol 55 (3) ◽  
pp. 229 ◽  
Author(s):  
Dachriyanus Salni ◽  
M. V. Sargent ◽  
B. W. Skelton ◽  
I. Soediro ◽  
M. Sutisna ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Single Crystal ◽  
Ethyl Acetate Extract ◽  
Significant Activity ◽  
Structural Determination ◽  
X Ray ◽  
And Staphylococcus Aureus ◽  
Rhodomyrtus Tomentosa

The ethyl acetate extract of the leaves of Rhodomyrtus tomentosa (Ait.) Hassk. yielded rhodomyrtone (5) [6,8-dihydroxy-2,2,4,4-tetramethyl-7-(3-methyl-1-oxobutyl)-9-(2-methylpropyl)-4,9-dihydro-1H-xanthene-1,3(2H)-di-one] which had significant activity against Escherichia coli and Staphylococcus aureus. The structural elucidation followed from spectroscopic evidence and from a single-crystal X-ray structural determination.

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