scholarly journals Evaluation of hepatoprotective potential of methanolic extract of the plant dolichos biflorus linn

2016 ◽  
Vol 2 (5) ◽  
pp. 116
Author(s):  
Deepthi B ◽  
Ashoka Shenoy M ◽  
Karunakar Hegde
Keyword(s):  
Body Weight ◽  
Liver Injury ◽  
Methanolic Extract ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Dolichos Biflorus ◽  
Liver Functions ◽  
Dose Dependent ◽  
Endogenous Enzymes

Present study is to evaluate the hepatoprotective potential of methanolic extract of the plant Dolichos biflorus Linn. against paracetamol and alcohol induced hepatotoxicity in rats. Oral administration of plant extract in two doses 200mg/kg and 400mg/kg body weight were subjected for the evaluation of hepatoprotective potential against alcohol (2ml/100g) and PCM (2g/kg) induced liver injury. Silymarin (25mg/kg) was used as a standard drug. The parameters like SGPT, SGO, ALP, TB and endogenous enzymes were estimated to assess the liver functions. In addition histopathological study was also carried out. Both the lower (200mg/kg) and higher dose (400mg/kg) of D.biflorus extract showed dose dependent significant decrease in SGPT, SGOT, ALP and TB levels when compared with toxic control. Both extracts showed decrease in LPO and increase in GSH, SOD and CAT levels. Hepatoprotective effect was also confirmed by histopathological analysis of liver which showed less damage in extract treated rats. The results obtained were comparable with that of the standard. The present study concluded that Dolichos biflorus Linn. plant were found to be effective against hepatotoxicity induced by Alcohol and Paracetamol.

scholarly journals Evaluation of hepatoprotective effect of Waltheria indica against various NSAIDs-induced hepatic damage in rats

2017 ◽  
Vol 9 (1) ◽  
pp. 157 ◽  
Author(s):  
Amol Chandekar ◽  
Neeraj Upamanyu ◽  
Amber Vyas ◽  
Atul Tripathi ◽  
Surendra Agrawal ◽  
...  
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Traditional Medicine ◽  
Hepatic Fibrosis ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Blood Samples ◽  
Liver Disorders

<p>The objective of the present study was to evaluate methanolic extract of leaves of Waltheria indica linn. for hepatoprotective potency of the potent solvent extract. The hepatotoxicity was induced by diclofenac, carbon tetrachloride (CCl 4 ) and acetaminophen. In CCl 4 induced hepatotoxicity study, animals were divided into five groups (n=6). Methanolic extract of Waltheria indica (WIM) groups were injected in doses of 400 mg/kg and 600mg/kg body weight along with CCl 4 and Silymarin 100mg/kg was taken as standard drug. Similarly procedure was followed in diclofenac and acetaminophen induced hepatotoxicity. Blood samples and liver were collected and liver hisopathological studies were carried out. These histopathological analysis suggested that WIM extract have the ability to reduce the degree of hepatic fibrosis induced by various factors. And concluded that WIM extract has significant hepatoprotective activity thus this study scientifically support the theory to use of this plant in traditional medicine for the treatment of liver disorders.</p>

PHYTOCHEMICAL AND BIOLOGICAL STUDIES OF ROTULA AQUATICA LOUR. ROOTS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 34-38
Author(s):  
T. Shyam ◽  
◽  
S Ganapaty
Keyword(s):  
Body Weight ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Biological Studies ◽  
Bacteria And Fungi ◽  
Dose Levels ◽  
Dose Dependent ◽  
Chemical Evidence

Four compounds viz α-amyrin, β- amyrin, bauerenol and ellagic acid were isolated from the methanolic extract of Rotula aquatica roots. The structures of these compounds were elucidated on the basis of spectroscopic data analysis and chemical evidence. The extract was evaluated for hepatoprotective activity against carbon tetrachloride induced hepatotoxic model at a dose levels of 200,400 and 800 mg/ kg body weight and compared with that of the standard silymarin (25mg/kg body weight). It showed good hepatoprotective activity in a dose dependent manner. The extract was also screened for antimicrobial activity against various types of organisms like bacteria and fungi.

scholarly journals Hepatoprotective effects of Sapium sebiferum in paracetamol-induced liver injury

2015 ◽  
Vol 10 (2) ◽  
pp. 393 ◽  
Author(s):  
Liaqat Hussain ◽  
Muhammad Sajid Hamid Akash ◽  
Madeha Tahir ◽  
Kanwal Rehman
Keyword(s):  
Liver Injury ◽  
Serum Levels ◽  
Therapeutic Effects ◽  
Sapium Sebiferum ◽  
Dependent Manner ◽  
Protective Effects ◽  
Drug Induced ◽  
Standard Drug ◽  
Hepatoprotective Effects ◽  
Dose Dependent

<span><em>Sapium sebiferum</em> leaves were used to determine its hepatoprotective effects against paracetamol-induced hepatotoxicity in mice. A dose dependent study was conducted using two different doses (200 mg/kg and 400 mg/kg) of the extract of </span><em>S. sebiferum</em><span> against toxic effects of paracetamol (500 mg/kg) in experimental animal model. Silymarin (50 mg/kg) was used as standard drug to compare therapeutic effects of </span><em>S. sebiferum</em><span> with control and paracetamol-treated groups. Paracetamol significantly increased the serum levels of liver enzyme markers like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, and direct bilirubin. The extract showed protective effects by normalizing the liver enzymes markers in a dose dependent manner. Histopathological results confirmed the hepatoprotective effects of leaves of </span><em>S. sebiferum</em><span>. We conclude that leaves of </span><em>S. sebiferum</em><span> have strong hepatoprotective effects against paracetamol-induced liver injury and can be used in liver injuries caused by drug-induced toxicity.</span>

Antidiabetic and antioxidative properties of the hydro-methanolic extract (60:40) of rhizomes of Curcuma amada roxb. (Zingiberaceae) in streptozotocin-induced diabetic male albino rat: a dose-dependent study through biochemical and genomic approaches

2019 ◽  
Vol 16 (4) ◽  
Author(s):  
Dipanwita Mitra ◽  
Riya Sarkar ◽  
Debidas Ghosh
Keyword(s):  
Body Weight ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Dependent Manner ◽  
Male Adult ◽  
Curcuma Amada ◽  
Corrective Effect ◽  
Dose Dependent

Abstract Background Curcuma amada is the most popular traditional medicine in India for the treatment of diabetes. The present study aimed to focus the antidiabetic and antioxidative activity of C. amada through the analysis of biochemical and genomic levels in a dose-dependent manner in streptozotocin-induced male adult rat. Method Streptozotocin-induced diabetic rats were administered orally with hydro-methanolic extract of C. amada at the dose of 10, 20, 40 and 80 mg/100 g body weight of rats for 28 days. The antidiabetic and antioxidative efficacy of the extract on glycemic, enzymatic, genomic and histological sensors along with toxicity study was investigated. Results The result showed a significant antidiabetic and antioxidative effect of the extract at dose-dependent manner. The significant recovery of fasting blood glucose level, serum insulin, activity of carbohydrate metabolic enzymes and antioxidative enzymes in extract-treated diabetic group as compared to untreated diabetic group were noted. After the extract treatment, the size of pancreatic islet and cell population densities were significantly increased. Activities of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in liver were significantly recovered along with the correction of Bax and Bcl-2 gene expression in hepatic tissue after the extract treatment in diabetic rats in respect to untreated diabetic group. Out of all the doses, the significant effects were noted at the dose of 20 mg/100 g body weight which has been considered as threshold dose in the concern. Conclusion It may be concluded that the significant and corrective effect in most of the sensors was noted at the minimum dose of 20 mg/100 g body weight of hydro-methanolic extract of C. amada without producing any toxicity.

scholarly journals In VivoAntiplasmodial and Analgesic Effect of Crude Ethanol Extract ofPiper guineenseLeaf Extract inAlbinoMice

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
A. Y. Kabiru ◽  
G. F. Ibikunle ◽  
D. A. Innalegwu ◽  
B. M. Bola ◽  
F. M. Madaki
Keyword(s):  
Body Weight ◽  
Analgesic Efficacy ◽  
Ethanol Extract ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Piper Guineense ◽  
Standard Drug ◽  
Analgesic Effects ◽  
Dose Dependent

Antiplasmodial and analgesic effects of crude ethanol extract ofPiper guineensewas investigated in mice. The antiplasmodial and analgesic efficacy of the extract was judged on its ability to reduce parasitemia and writhing, respectively, in mice. The antiplasmodial screening involved treating infected mice with 200, 400, and 600 mg/kg body weight of extract while the positive control group was given standard artesunate drug. The analgesic test was carried out by administering 1000, 1500, and 2000 mg/kg body weight of extract to three groups of healthy mice, respectively, after induction of pain with 0.75% acetic acid. The positive control group was given aspirin drug. Parasitemia was reduced by 28.36%, 43.28%, and 62.69% in a dose-dependent pattern in the curative test which was significantly different (P<0.05) from 96.03% of the standard drug. The reduction of writhing by mice given the extract was also dose-dependent (36.29, 45.43, and 59.07%). Aspirin drug was however more effective (86.36%). The extract was safe at 2000 mg/kg body weight. Phytochemical screening revealed the presence of flavonoids, tannins, phlobatannins, terpenoids, and coumarins. Result obtained in this study demonstrated the efficacy of ethanol extract ofPiper guineenseas an antiplasmodial and analgesic agent.

scholarly journals Toxicity evaluation and protective effect of Rhus longipes Engl. leaf extract in paracetamol induced oxidative stress in wister rats

2017 ◽  
Vol 6 (2) ◽  
pp. 73-77
Author(s):  
Olubukola S. Olorunnisola ◽  
◽  
Adewale Adetutu ◽  
Abiodun O. Owoade ◽  
Babatunde T. Adesina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Protective Effect ◽  
Leaf Extract ◽  
Control Group ◽  
P Value ◽  
Standard Drug ◽  
Test Of Significance ◽  
Dose Dependent ◽  
Isolated Liver

Aim: Acute toxicity and protective effect of ethanol leaf extract of Rhus longipes Engl. against Paracetamol induced oxidative stress was investigated. The LD50 of the leaf extract was determined using up and down technique and the effect of 1/10th and 1/20th/ LD50 of the extract on antioxidants enzymes and non-enzymes were assessed in the serum and isolated liver of normal and Paracetamol intoxicated rats. Data obtained were analyzed by one-way analysis of variance (ANOVA) and Dunnett’s t-test was used as the test of significance. Values were considered significant at P value < 0.05. The results obtained indicated that LD50 of Rhus longipes Engl. leaf extract is greater than 5000 mg/kg /body weight. A significant (p<0.05) increase was observed in the level of hepatic (H) TBARs (81.97%), Catalase (38.42%) and serum (S) TBARs (164.44%) and catalase (64.72%) respectively but, a significant (P<0.05) decrease in hepatic activities of SOD, GPX, GR, vitamin C and E in paracetamol treated groups when compared with the serum and normal control group respectively. The extracts (250 and 500 mg/kg/body/weight) and the standard silymarin significantly (p<0.05) restored the derange antioxidants parameters to near normal in dose dependent manners. The activities of the extract at the highest concentration (500 mg/kg/b.wt) compared favourably with the standard drug. The results suggested that the leaf extract of Rhus longipes Engl. contain bioactive compounds which could protect against toxicity induced oxidative stress. The results of this study can be used as a basis for further investigations in the search for the bioactive principle.

scholarly journals Preclinical Appraisal of Hematinic Potential of Mandura Bhasma for Treating Anemia

2021 ◽  
pp. 207-214
Author(s):  
Deepak S. Khobragade ◽  
Mrunali S. Potbhare ◽  
Awdhut Pimpale ◽  
Sagar B. Wankhede ◽  
Chandrashekhar R. Tempe
Keyword(s):  
Body Weight ◽  
Ferrous Sulphate ◽  
Standard Drug ◽  
Dependent Activity ◽  
Daily Dose ◽  
Study Institute ◽  
Dose Dependent ◽  
Pharmaceutical Education ◽  
Different Doses

Aims: To evaluate hematinic potential of mandura bhasma. Study Design: Experimental study. Place and Duration of Study: Institute of Pharmaceutical Education and Research, Wardha, Maharashtra, India. 6 Months. Methodology: The anti-anaemic potential of Mandura bhasma in Wistar rats was investigated. Anaemia was induced in rats with phenyl hydrazine hydrochloride at a dose of 10        mg kg-1 body weight by oral administration. Anaemia was treated with mandura bhasma administered in three different doses based on body weight. Results: In vivo investigation showed that though the dose of 6mg kg-1 body weight of mandura bhasm produced only a minimal antianaemic (hematinic) effect, oral daily dose of 11 mg kg-1 body weight and 22mg kg-1 body weight a produced a significant (P < 0.05) antianaemic effect when compared to standard drug ferrous sulphate indicating dose dependent activity. Conclusions: The results indicate that Mandura Bhasm have very potential dose dependant hematinic activity and can be a safe and effective drug for treating anemia.

scholarly journals Antidiarrhoeal activity of rind of Punica granatum

2013 ◽  
Vol 2 (5) ◽  
pp. 101-104 ◽  
Author(s):  
Saleha Akter ◽  
Amit Sarker ◽  
Md. Sanowar Hossain
Keyword(s):  
Body Weight ◽  
Animal Models ◽  
Magnesium Sulfate ◽  
Castor Oil ◽  
Methanolic Extract ◽  
Punica Granatum ◽  
Pharmaceutical Journal ◽  
Phytochemical Screening ◽  
Traditional Use ◽  
Standard Drug

Punica granatum (Family: Punicaceae) is a widely consumed fruit in Bangladesh locally known as Anar or Bedana. Traditionally the plant is used to treat diarrhoea, dysentery, hemorrhage, tuberculosis and chronic periodontitis. The present study was undertaken in order to justify and authenticate the traditional use of rind of P. granatum fruit in diarrhoea. Methanolic extract of the rind of the fruit was tested in castor oil- and magnesium sulfate-induced diarrhoea in mice at the doses of 200 and 400 mg/kg body weight. The results of the present study revealed significant antidiarrhoeal activity (p<0.001) in both animal models. The extract inhibited 31.25% defaecation at the dose of 200 mg/kg and 53.75% at the dose of 400 mg/kg in castor oil-induced diarrhoea while standard drug loperamide inhibited 71.25% defaecation. In magnesium sulfate-induced diarrhoea the inhibition of defaecation was 45.71 and 57.14% at the doses of 200 and 400 mg/kg respectively while loperamide inhibited 64.29% defaecation. The antidiarrhoeal effect of the extract was concentration dependent in both castor oil-induced diarrhoea and magnesium sulfate-induced diarrhoea. Phytochemical screening of the extract revealed the presence of flavonoids and alkaloids that may play key role in its antidiarrhoeal activity. The results of the present study validate the folkloric use of the rind of P. granatum in the treatment of diarrhoea.DOI: http://dx.doi.org/10.3329/icpj.v2i5.14437 International Current Pharmaceutical Journal, April 2013, 2(5): 101-104

scholarly journals Screening of analgesic activity of Phoenix sylvestris leaves in rodents

2018 ◽  
Vol 4 (1) ◽  
pp. 22-24
Author(s):  
Pankaj Jain ◽  
◽  
Sonika Jain ◽  
Surendra Kumar Swarnkar ◽  
Swapnil Sharma ◽  
...  
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Leaf Extract ◽  
Methanolic Extract ◽  
Standard Drug ◽  
Swiss Albino Mice ◽  
Tail Immersion ◽  
Albino Mice ◽  
Withdrawal Reflexes

Aim: The present study evaluated the central and peripheral analgesic activity of methanolic leaf extract of Phoenix sylvestris (PSLME) in swiss albino mice. Method: Peripheral and central analgesic activity was evaluated by tail immersion and acetic acid writhing in swiss albino mice. Dextropropoxyphene was used as a standard drug in the dose of 65mg/kg body weight in both models. PSLME was tested at 100 and 500mg/kg dose level. Results: The result revealed that methanolic extract exhibit 48% and 40.5% writhing inhibition at 500 and 100 mg/kg doses whereas ~30% tail withdrawal reflexes inhibition at 500mg/kg which was analogous to the standard drug dextropropoxyphene. Conclusion: Methanolic extract of leaves of P. sylvestris possesses both peripheral and central analgesic activity in experimental animal.

scholarly journals Antinociceptive Activity of Methanol Extract of Tamarix indica

1970 ◽  
pp. 1-3
Author(s):  
MAM Sarker ◽  
Md Abdul Matin Sarker
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Reducing Sugars ◽  
Diclofenac Sodium ◽  
Antinociceptive Activity ◽  
Standard Drug ◽  
Swiss Albino Mice ◽  
Phytochemical Investigation ◽  
Albino Mice ◽  
Dose Dependent

The antinociceptive activity of the methanolic extract of the barks of Tamarix indica Roxb. was investigated using acetic acid-induced writhing model in swiss albino mice. The extract produced about 26.89%( P< 0.01) and 43.55% (P< 0.001) writhing inhibition at the dose of 250 and 500 mg/ kg of body weight respectively, which was comparable to the standard drug diclofenac sodium where the inhibition was about 63.64% (P< 0.001) at the dose of 25 mg/kg of body weight. At the above doses, the extract exhibited significant and dose dependent antinociceptive activity in acetic acid-induced writhing model in swiss albino mice. Phytochemical investigation of the extract indicate the presence of reducing sugars, tannins, gums, flavonoids and saponin. Key words: Tamarix indica; antinociceptive activity; acetic acid-induced writhing; phytochemical test. DOI: 10.3329/bjpp.v25i1.5736Bangladesh J Physiol Pharmacol 2009; 25(1&2) : 1-3

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