Detection of Human Parvovirus B19 Infection in Children with Chronic Haemolytic Anaemia in Benha University Hospitals

2021 ◽  
Vol 30 (2) ◽  
pp. 51-58
Author(s):  
Amal M. Matta ◽  
Elsayed M. Abd-Elghany ◽  
Abeer A. Aboelazm ◽  
Osama Abo. Zaki, ◽  
Doaa Abd. Shaker
Keyword(s):  
Hemolytic Anemia ◽  
Parvovirus B19 ◽  
Control Group ◽  
P Value ◽  
Healthy Children ◽  
Igg Antibodies ◽  
University Hospitals ◽  
History Of ◽  
Chronic Hemolytic Anemia ◽  
Apparently Healthy

Background: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as thalassemia, hereditary spherocytosis, sickle cell disease and Glucose-6-phosphate dehydrogenase deficiency leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. Objectives: Detection of parvovirus B19 DNA and its IgG antibodies in the serum of children with chronic hemolytic anemia and in apparently healthy children in Benha University Hospitals. Methodology: The study was conducted on 80 children. Forty of them with chronic hemolytic anemia, they were subdivided into 2 groups, Group (1a) included 20 patients without history of aplastic crisis, Group (Ib) included 20 patients with a history of aplastic crisis and 40 age and sex-matched apparently healthy children representing control (Group II). All patients were subjected to full history taking, clinical examination and laboratory investigations. Parvovirus B19 IgG was measured using anti-parvovirus B19 ELISA kits (SUNRED), and parvovirus B19 DNA was detected by using nestedpolymerase chain reaction. Results: The seroprevalence of parvovirus B19 IgG was significantly higher (P value =0.016) in Group Ia (50%) (10 out of 20) and Group Ib (45%) (9 out of 20) than the control group (Group II) (17.5%) (7 out of 40). There was a significant positive correlation between anti-parvovirus B19 IgG and age of all patients, frequency of blood transfusion. The prevalence of parvovirus B19 DNA was 10% (2 out of 20) in group Ia and 30% (6 out of 20) in group Ib and no viral DNA was detected in the controls (P value=0.001). Although 42.3% (11 out of 26) of children with β thalassemia major had a detectable level of antiparvovirus B19 virus IgG antibodies, only (23.1%) (6 out of 26) of them had B19 DNA. Anti-parvovirus B19 IgG antibodies were detected in 4 children out of 5 children of sickle cell anemia (80%) but the the prevalence of Parvovirus B19 DNA was 20% among them. Conclusion: Measures to keep away from iatrogenic and nosocomial infection transmission should be implemented including screening of donated blood for parvovirus B19 especially blood given to patients with blood disorders. Recommendation: Data from this study support the need for introduction of an approved vaccine that mainly protects children with chronic hemolytic anemia against that infection.

scholarly journals Study of chronic hemolytic anaemia patients in Rio de Janeiro: prevalence of anti-human parvovirus B19 IgG antibodies and the developement aplastic crises

2002 ◽  
Vol 44 (4) ◽  
pp. 187-190 ◽  
Author(s):  
Anadayr L.M. SANT'ANNA ◽  
Rita de Cássia N. Cubel GARCIA ◽  
Mônica MARZOCHE ◽  
Heloisa Helena A. Gallo da ROCHA ◽  
Maria Tereza M. PAULA ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Hemolytic Anemia ◽  
Sickle Cell ◽  
Parvovirus B19 ◽  
Human Parvovirus B19 ◽  
Igg Antibodies ◽  
Cell Disease ◽  
Antibody Prevalence ◽  
Igg Antibody ◽  
Chronic Hemolytic Anemia

The prevalence of anti-human parvovirus B19 IgG antibodies was determined in sera from 165 chronic hemolytic anemia patients, receiving medical care at Instituto Estadual de Hematologia (IEHE), Rio de Janeiro, during the year of 1994. This sample represents around 10% of the chronic hemolytic anemia patients attending at IEHE. Most of these patients (140) have sickle cell disease. Anti-B19 IgG antibodies were detected in 32.1% of patients. No statistically significant difference (p > 0.05) was seen between IgG antibody prevalence in male (27.8%) and female (35.5%) patients. Anti-B19 IgG antibodies were more frequent in older (37.6%) than younger (28.2%) than 20 years old patients, although this difference had no statistical significance (p > 0.05). Anti-B19 IgG antibody prevalence showed that 67.9% of patients enrolled in the study were susceptible to B19 acute infection. With the aim to detect acute B19 infection, patients follow up continued until February 1996. During this period four patients presented transient aplastic crisis due to human parvovirus B19 as confirmed by the detection of specific IgM antibodies. All four patients were younger than 20 years old, and 3 were younger than 10 years old. Three of them were sickle cell disease patients. Three of the four acute B19 infection occurred during 1994 springtime.

scholarly journals Seroprevalence of Antibodies against SARS-CoV-2 in Children with Juvenile Idiopathic Arthritis a Case-Control Study

2021 ◽  
Vol 10 (8) ◽  
pp. 1771
Author(s):  
Violetta Opoka-Winiarska ◽  
Ewelina Grywalska ◽  
Izabela Korona-Glowniak ◽  
Katarzyna Matuska ◽  
Anna Malm ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Disease Activity ◽  
Disease Activity Score ◽  
Juvenile Arthritis ◽  
Activity Score ◽  
Control Group ◽  
Healthy Children ◽  
Serum Samples ◽  
History Of ◽  
Novel Coronavirus

There is limited data on the effect of the novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) on pediatric rheumatology. We examined the prevalence of antibodies against SARS-CoV-2 in children with juvenile idiopathic arthritis (JIA) and a negative history of COVID-19 and the correlation of the presence of these antibodies with disease activity measured by juvenile arthritis disease activity score (JADAS). In total, 62 patients diagnosed with JIA, under treatment with various antirheumatic drugs, and 32 healthy children (control group) were included. Serum samples were analyzed for inflammatory markers and antibodies and their state evaluated with the juvenile arthritis disease activity score (JADAS). JIA patients do not have a higher seroprevalence of anti-SARS-CoV-2 antibodies than healthy subjects. We found anti-SARS-CoV-2 antibodies in JIA patients who did not have a history of COVID-19. The study showed no unequivocal correlation between the presence of SARS-CoV-2 antibodies and JIA activity; therefore, this relationship requires further observation. We also identified a possible link between patients’ humoral immune response and disease-modifying antirheumatic treatment, which will be confirmed in follow-up studies.

Indicators of the kinin blood system in severe forms of acute respiratory viral infections in children

1982 ◽  
Vol 63 (2) ◽  
pp. 51-52
Author(s):  
V. A. Anokhin ◽  
A. D. Tsaregorodtsev
Keyword(s):  
Viral Infection ◽  
Viral Infections ◽  
Severe Form ◽  
Blood System ◽  
Control Group ◽  
Healthy Children ◽  
Respiratory Viral Infection ◽  
Respiratory Viral Infections ◽  
Acute Respiratory Viral Infection ◽  
Apparently Healthy

The aim of this work was to study the parameters of the components of the kinin blood system in children with severe forms of acute respiratory viral infections (ARVI) with neurotoxicosis syndrome. 55 children with ARVI (aged from 1 to 6 months - 14, from 6 months to 1 year - 18, from 1 to 3 years - 11, from 3 to 7 years - 12). 38 patients were admitted in the first three days of illness, 12 - on 4-5 days and 5 - at a later date. 30 children had a severe form of acute respiratory viral infection and 25 - moderate. Adenovirus infection was diagnosed in 14 patients, influenza - in 16, parainfluenza - in 7, MS-viral infection in 5, mixed viral infection - in 13. The control group consisted of 10 apparently healthy children.

scholarly journals Recurrent Miscarriage and Consanguinity among Omani Women– A Cross Sectional Study

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Fatma Al Hoqani ◽  
Wadha Al Ghafri ◽  
Saneya El tayeb ◽  
Yahya Al Farsi ◽  
Vaidyanathan Gowri
Keyword(s):  
Recurrent Miscarriage ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Control Group ◽  
P Value ◽  
Cross Sectional ◽  
Recurrent Miscarriages ◽  
Sultan Qaboos University ◽  
Chi Squared ◽  
History Of

Objective: to determine the prevalence of explained and unexplained recurrent miscarriages (RM) and to find out if there is a significant relationship between recurrent miscarriages and consanguinity. Methods: A cross sectional in which the cases group included all women with RM attending the outpatient clinic at Sultan Qaboos University Hospital from July 2006 to April 2012 and the controls group included women with no history of RM after matching them with cases for age (case to control ratio was 1:1). The main outcome measures were the prevalence of consanguinity in women with or without recurrent miscarriages. Results: During study period a total of 290 women with RM were seen. Of which, 150 (51.7%) women had unexplained RM. Control group with no history of RM were 300 women. Consanguinity rate among cases (49.5%) %) was less than the controls (52.7 %%). Both first cousin and second cousin marriages were more common in the controls than the cases and it was not statistically significant (p value 0.476, chi squared test). Conclusion: In this study we found that more than half of RM cases were unexplained and there was no significant association between RM and consanguinity.

scholarly journals Quality of Life in Children with Asthma versus Healthy Children

2018 ◽  
Vol 6 (8) ◽  
pp. 1413-1418 ◽  
Author(s):  
Samaneh Kouzegaran ◽  
Parisa Samimi ◽  
Hamid Ahanchian ◽  
Maryam Khoshkhui ◽  
Fatemeh Behmanesh
Keyword(s):  
Quality Of Life ◽  
Physical Performance ◽  
Control Group ◽  
P Value ◽  
Healthy Children ◽  
Significant Difference ◽  
Children With Asthma ◽  
And Performance ◽  
Asthma Group

BACKGROUND: Asthma is the most prevalent chronic disease in the pediatric age group. The disease affects different aspects of the children's lives, such as physical, emotional, social and educational aspects. Thus, more focus has been on the quality of life in these patients rather than the duration of their illness in recent years.AIM: This study examined the different aspects of quality of life in asthmatic children for the first time in this geographic area.METHODS: The study was cross-sectional conducted in 2015-2016. The asthmatic group was 100 patients aged 8 to 12 admitted to the Asthma and Allergy Clinic of Ghaem Hospital (as) in Mashhad with the control group composed of 100 healthy children of the same age and gender. The standard questionnaire pedsQLTM was used for comparing the quality of life of children in the two groups. Statistical analysis was SPSS23 with P-value less than 0.05, which was statistically significant.RESULTS: In each group, 58 patients were boys, and 42 were girls. In a comparison of the quality of life of children, the asthma group with a mean total score of Peds QL 20.99 ± 12.54 compared to the healthy children with a mean total score of Peds QL of 8.8 ± 5.41 had a lower quality of life (P < 0.001). Moreover, regarding various aspects of quality of life asthma group had a lower quality of life in physical performance, emotional performance and performance in school (P < 0.001). Nonetheless, there was no significant difference between the two groups considering social function (P = 0.267). Examining the relationship between Peds QL score of patients with asthma with various variables was indicative of the fact that Peds QL scores were significantly correlated with the gender of the patients, showing better quality of life in the girls (P = 0.001).CONCLUSION: The results indicated that children with asthma have a significantly lower quality of life compared with healthy children of the same age. Also, in examining the different aspects of quality of life, these children had a lower quality of life in physical performance, emotional performance, and performance at school, and were at the level as that of healthy children only in social performance.

Motor development in children with congenital cardiac diseases compared to their healthy peers

2007 ◽  
Vol 17 (5) ◽  
pp. 487-498 ◽  
Author(s):  
Birna Bjarnason-Wehrens ◽  
Sigrid Dordel ◽  
Sabine Schickendantz ◽  
Constanze Krumm ◽  
Daniel Bott ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Motor Development ◽  
Control Group ◽  
P Value ◽  
Healthy Children ◽  
Parental Overprotection ◽  
Coordination Test ◽  
Congenital Cardiac Disease ◽  
The Mean ◽  
And Gender

AbstractTheir perceptual and motor experiences determine the physical and motor development of children, and impact also on their emotional, psychosocial, and cognitive development. Our aim, therefore, was to evaluate motor development in children with congenitally malformed hearts compared to their healthy peers.We compared 194 children, with a mean age of 10.0 years, and standard deviation of 2.7 years, representing the entire spectrum of congenital cardiac disease, to a control group of 455 healthy children, having a mean age 9.6 years, with standard deviation of 2.17 years. The bodily coordination test for children was used to examine motor development.Of the children with congenitally malformed hearts, 26.8% showed moderate, and 31.9% had severe disturbances of motor development, compared to 16.5% and 5.5% of the control group, the p-value for these differences being less than 0.001. The mean motor quotient adjusted for age and gender was lower in the children with congenitally malformed hearts than in their healthy peers, at 79.6, with standard deviation of 18.9 as opposed to 96.6, with standard deviation of 15, this difference having a p-value of less than 0.001. Depending on the presence, and/or the degree, of residual sequels, the children with congenitally malformed hearts were divided into two subgroups, with either no or mild residual sequels, or with significant sequels. The mean motor quotient was lower in those with significant residual sequels, at 75, with standard deviation of 19.3, as opposed to 83, with standard deviation of 17.9, the p-value for this difference being less than 0.01. In both subgroups, the mean motor quotient was lower, with a p-value of less than 0.01, than in the control group.Our findings show that children with congenitally malformed hearts have deficits in their motor development, these being found in the presence of no or mild sequels, as well as with significant residual sequels. Parental overprotection may contribute to these findings.

scholarly journals Evaluation of The 10 Warning Signs In Immunodeficient Patients: Additional Suggestions

2021 ◽  
Author(s):  
Fadime Ceyda Eldeniz ◽  
Yahya Gül ◽  
Alaattin Yorulmaz ◽  
Şükrü Nail Güner ◽  
Sevgi Keles ◽  
...  
Keyword(s):  
Family History ◽  
Early Diagnosis ◽  
Chronic Diarrhea ◽  
Warning Signs ◽  
Control Group ◽  
Healthy Children ◽  
Physician Awareness ◽  
History Of ◽  
And Control ◽  
Immunological Evaluation

Abstract Objective: Ten warning signs of primary immunodeficiency (PID) were suggested by the Jeffrey Modell Foundation (JMF), to increase physician awareness of PID. These warning signs have not yet been evaluated for patients with secondary immunodeficiency (SID). This study investigated whether the 10 warning signs used for the diagnosis of PID are sufficient for the diagnosis of SID, and explored the possibility of additional signs.Methods: This prospective study was conducted between June and December 2020. The mothers of 162 patients with PID and SID, and mothers of 200 healthy children, were asked to complete a questionnaire about family and personal history in addition to the warning signs of PID developed by the JMF. A JMF score was created by giving one point for each “Yes” answer for the 10 warning signs of PID. Medical records of the patients were evaluated for possible additional warning signs for PID and SID. Results: The JMF scores of the PID (3.36 ± 1.65) and SID (3.72 ± 1.12) groups were significantly higher than the scores of the control group (0.34 ± 0.61) (p < 0.05). A sign for immunological evaluation in two patients without warning signs in the PID group was found to be chronic diarrhea. In addition to the 10 JMF warning signs, we found that consanguinity and a family history of tuberculosis were statistically significant in our PID group, compared with the SID and control groups. Conclusions: The JMF warning signs are important for early diagnosis of PID. Our study showed that these signs may also be used for the early diagnosis of SID in patients and, according to our results, in addition to the 10 JMF signs for PID, parental consanguinity, chronic diarrhea, and a family history of tuberculosis may also be considered warning signs for the early diagnosis of PID.

scholarly journals Study of correlation between breast feeding and wheezing in children

2018 ◽  
Vol 6 (1) ◽  
pp. 191
Author(s):  
Sagar Potharajula ◽  
Shreedhara Avabratha Kadke
Keyword(s):  
Family History ◽  
Breast Feeding ◽  
Cow’S Milk ◽  
Control Group ◽  
P Value ◽  
Chi Square ◽  
College Hospital ◽  
Cow's Milk ◽  
Breast Feed ◽  
History Of

Background: Wheezing is the most common chronic health problem in childhood. Many studies have found protective effect of breastfeeding on wheezing, while few others have not. Hence this study was taken up to find out the correlation between breastfeeding and wheezing in children.Methods: Case control study done in a Medical College Hospital. Cases were children of 2-5yrs age with history of wheezing or who had received nebulisation. Controls were children without history of wheezing or nebulisation. Mothers were interviewed with a predesigned proforma. Results were analysed by Chi square test and Odd’s ratio and p value <0.05 was considered significant.Results: There were 92 cases and 184 controls. Sixteen (53.4%), 48 and 27 cases were breastfed less than 1year, till 2yrs and beyond 2yrs respectively. 14 (46.6%), 128, and 40 were the numbers in control group. Breast feeding duration till 1 year of age is statistically correlated with wheezing with p value of 0.02, higher proportion of children who were breastfed for less than 1 year had wheezing when compared to controls. 26 cases out of 69 were not exclusively breast fed till 6 months and 66 cases out of 207 were exclusively breastfed. Higher proportion of cases who were not exclusively breastfed had developed wheeze although it was not significant statistically. 45 cases (36.8%) were initiated on cow’s milk before 1yr of age, 47 cases (30.5%) were initiated after 1year. Higher proportion of children who were initiated on cow’s milk early had developed wheeze but was not significant statistically. 34 cases and 24 controls had family history of asthma. With family history wheezing episodes were 3.72 times more likely irrespective of breast-feeding duration.Conclusions: Breast feeding gives protection against wheezing in children. Mothers should be encouraged to breast feed their children.

scholarly journals Tuberculin sensitivity among inmates of children’s home

2017 ◽  
Vol 4 (5) ◽  
pp. 1581
Author(s):  
Usha Kiran C. B. ◽  
Sowmya J. ◽  
Jayamala R.
Keyword(s):  
Early Diagnosis ◽  
Nutritional Status ◽  
Vaccination Status ◽  
Healthy Children ◽  
Sensitivity Testing ◽  
Feasible Method ◽  
Tuberculin Sensitivity ◽  
Children's Home ◽  
History Of ◽  
Apparently Healthy

Background: Tuberculin sensitivity and its relationship to nutritional status in inmates of children's home.Methods: Tuberculin sensitivity was checked in 180 apparently healthy orphan children and its relationship with nutritional status, previous vaccination status was studied. setting: children's home run by NGOs participants: all inmates of children's home aged 2-15 years who meet inclusion and exclusion criteria.Results: Out of 180 apparently healthy children 27 (15%) were tuberculin positive. 109 (60.5%) were undernourished children out of which 15 (55.56%) were tuberculin positive. Out of 71 normal nourished children, 12 (44.4%) were tuberculin positive. BCG scar was present in 120 (66.6%) children out of which 19 were tuberculin positive and BCG scar was absent in 60% children out of which 8 were tuberculin positive. 8 children had history of contact with TB and 6 of them were tuberculin positive.Conclusions: Tuberculin sensitivity testing using 5 TU PPD-RT23 is a simple feasible method to screen inmates of children home at the time of entry and it is not influenced by nutritional status or previous BCG vaccination. Early diagnosis and treatment of TB will go a long way in reducing the burden of TB in our country.

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