Character of β-lymphocytes differentiation in women with hypertensive disorders during pregnancy

2021 ◽  
Vol 66 (8) ◽  
pp. 489-495
Author(s):  
I. A. Panova ◽  
A. V. Kudryashova ◽  
A. S. Panashchatenko ◽  
E. A. Rokotyanskaya ◽  
A. I. Malyshkina ◽  
...  
Keyword(s):  
B Cells ◽  
General Population ◽  
Arterial Hypertension ◽  
B Lymphocytes ◽  
Elastic Properties ◽  
Vascular Wall ◽  
Relative Content ◽  
Control Group ◽  
Hypertensive Disorders ◽  
B1 Cells

The aim of the work was to identify the features of B-lymphocyte differentiation in women with hypertensive disorders of various origins, to establish their relationship with indicators of the elastic properties of the vascular wall, and to develop additional diagnostic criteria for the severity of preeclampsia. We examined 193 women at 24-40 weeks of gestation. Of these, 39 women with chronic arterial hypertension, 35 women with preeclampsia that developed against the background of chronic arterial hypertension, 55 with preeclampsia. The control group consisted of 64 women without hypertensive disorders. To assess the elastic properties of the vascular wall, a sphygmographic attachment of the “Poly-Spectrum-8” hardware-software complex (“Neurosoft” Ivanovo) was used. The relative content of B-lymphocytes (CD19 + and CD20 +), regulatory B-cells (CD20 + IL-10 +), switched (CD19 + CD27 + IgD-) and non-switched (CD19 + CD27 + IgD +) memory cells, plasma cells (CD19 + CD20-CD38 +) in the general population of B-lymphocytes was assessed by flow cytometry on a FACSCantoII flow cytometer using the FACS Diva program. Statistical analysis was carried out using the programs “Statistica for Windows 6.0”, “Microsoft Excel 2010” and “MedCals”. All subjects with hypertensive disorders showed an increase in the stiffness of the arteries of the muscular and elastic types, the most pronounced in the groups of patients with PE, the maximum in women with CAH and associated PE. An increased level of B1-lymphocytes in the peripheral blood is also noted in all hypertensive disorders. There were revealed positive correlations of high strength between: the level of CD20 + cells and the velocity of pulse wave propagation through the arteries of the muscular type (PWVm) in all groups with hypertensive disorders; the content of B1 cells and PWVM in moderate preeclampsia; level emory B-cells and PWV in elastic-type arteries in women with CAH and associated PE. The ROC analysis of the relative content of B1 cells in the general population of B lymphocytes and the content of IL-10-producing cells in the population of CD20 + lymphocytes (Breg) in women with moderate and severe PE revealed criteria for the differential diagnosis of preeclampsia of varying severity. The presence of hypertensive disorders of various origins is accompanied by a decrease in the elasticity of the arterial vascular wall, which is most pronounced in patients with CAH and associated PE. These changes are largely correlated with the level of B cells. As additional criteria for determining the severity of PE, the relative content of B1 cells and IL-10-producing cells in the population of Breg CD20 + lymphocytes can be used.

scholarly journals Evaluation of the elastic properties of the arterial wall in young patients with arterial hypertension

2005 ◽  
Vol 11 (1) ◽  
pp. 17-20 ◽  
Author(s):  
G. I. Storozhakov ◽  
G. S. Vereshchagina ◽  
Yu. B. Chervyakova ◽  
N. M. Fedotova
Keyword(s):  
Arterial Hypertension ◽  
Elastic Properties ◽  
Peripheral Resistance ◽  
Young Patients ◽  
Vascular Wall ◽  
Control Group ◽  
Young Males ◽  
Ultrasound Study ◽  
Elasticity Module ◽  
Technical Simplicity

To study early impairments in the elastic properties of the vascular wall in arterial hypertension (AH), 32 hypertensive patients of young age (18,9±2,6 years) were examined, a control group comprised 35 healthy young males of the same age Doppler ultrasound study (DUSS) of the common carotid (CC), brachial, and radial arteries was performed and CC wall elasticity modules, aortic compliance, and pulse wave velocity (PWV) were estimated Analyzing the results of DUSS in patients with AH revealed no significant differences in velocity parameters and in the values of peripheral resistance The estimation of the elasticity module at rest and during functional tests demonstrated a less increment in this parameter in patients with AH during exercise than in the control group Elasticity module changes were heteiodirectional during nitroglycerine tests The estimation of PWV revealed a significant increase in this parameter, which was indicative of early impairments in the elastic properties of the vascular wall The high informative \ alue and technical simplicity permit the use of PWV as a screening of vascular wall lesion in patients with AH

scholarly journals P0312ALTERATIONS IN THE PROGRESS OF LYMPHOCYTE APOPTOSIS IN PRE- AND POST- DIALYSIS END STAGE RENAL DISEASE PATIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Dimitra Vasileia Daikidou ◽  
MARIA STANGOU ◽  
Erasmia Sampani ◽  
Despoina Asouchidou ◽  
Vasiliki Nikolaidou ◽  
...  
Keyword(s):  
B Cells ◽  
Renal Disease ◽  
B Lymphocytes ◽  
End Stage Renal Disease ◽  
Statistical Significance ◽  
Control Group ◽  
Apoptotic Cells ◽  
Lymphocyte Apoptosis ◽  
Stage Renal Disease ◽  
End Stage

Abstract Background and Aims Lymphocyte apoptosis, as a programmed mechanism of lymphocyte death, is essential in maintaining homeostasis and balance between inflammatory and immune reactions. Disturbances in the apoptotic progress, leading to fragmented lymphocytes, “late apoptotic” cells, may result in immunodeficiency, oncogenesis, atheromatosis, etc. Aim of the present study was to investigate the lymphocyte apoptotic progress in End Stage Renal Disease (ESRD) and the effect of dialysis. Method The study included patients on ESRD; measurements were performed at the first day of dialysis (T0) and repeated 6 months later (T6), while being on dialysis. Total lymphocytes and B lymphocytes (CD19+) were gated and stained with Annexin V to detect apoptotic cells; early and late apoptotic cells were quantified. The results were compared to age-matched healthy control group. Results ESRD patients had reduced lymphocyte and B cell count, 1550±592μ/L vs. 2692±690μ/L, p<0.001 and 120.4±80μ/L vs. 321.7±184.7μ/L, p=0.002, respectively, compared to controls. There was an increase in total lymphocytes and B cells, being on later apoptotic stages (LAS) in ESRD-T0 compared to controls, 0.3±0.8% vs. 0.06±0.1%, and 0.04±0.08% vs. 0.01±0.03%, respectively, although differences did not reach statistical significance. After 6 months on dialysis, a reduction was noticed in the population of lymphocytes on LAS, 0.18±0.2% from 0.34±0.8%, while there was an increase of B cells on LAS, 0.1±0.2% from 0.02±0.07, with subsequent alterations in total numbers of apoptotic cells were also evident Conclusion Late apoptotic changes affecting total and particularly B lymphocytes happen in ESRD, and initiation of dialysis seem to cause further alterations, which may be implicated in the increased morbidity and mortality of disease

Patients with arterial hypertension and abdominal obesity: Biochemical predictors of a violation of the elastic properties of the vascular wall

2019 ◽  
Vol 493 ◽  
pp. S300-S301
Author(s):  
K. Avdeeva ◽  
T. Petelina ◽  
L. Gapon ◽  
N. Musikhina ◽  
L. Zyrianova ◽  
...  
Keyword(s):  
Arterial Hypertension ◽  
Elastic Properties ◽  
Abdominal Obesity ◽  
Vascular Wall

Plasma and Intracellular Concentration of TNF and Its Receptors in Peripheral Blood and Bone Marrow in Different Stages of B-CLL.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4781-4781
Author(s):  
Jacek Rolinski ◽  
Agnieszka Bojarska-Junak ◽  
Iwona Hus ◽  
Anna Dmoszynska
Keyword(s):  
Bone Marrow ◽  
T Cells ◽  
B Cells ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Biological Effects ◽  
Leukemic Cells ◽  
Control Group ◽  
Stage Of Disease ◽  
Positive Correlation

Abstract TNF has been proposed to play a role in the regulation of growth and death of leukemic B-CLL cells. However, the biological effects of TNF on leukemic cells, as well as its role as a prognostic factor need to be further investigated. The aim of the study was to eevaluate the correlation of TNF and its receptors in peripheral blood (PB) and bone marrow (BM) with the stage of B-CLL and some other clinical parameters. PB and BM were taken from 44 newly diagnosed, untreated B-CLL. patients. The control group consisted of 20 healthy subjects. We used flow cytometry technique to assess the capability of T and B lymphocytes to produce TNF and ELISA method to measure plasma levels of TNF and their soluble receptors. We found, that PB and BM plasma TNF concentration in the patients was significantly higher than in the healthy control (2.61 pg/ml. vs 0.62 pg/ml; and 2.91 pg/ml vs 0.75 pg/ml, respectively p<0.001). TNF concentration in PB and BM was significantly higher in Rai stage III–IV than in early stages (p<0.01). There was a correlation between the PB and BM TNF level and lymphocytosis (p<0.005) and the total tumor mass (TTM) (p<0.0001). The PB and BM TNF concentration positively correlated with the percentage of T CD3+ lymphocytes producing intracellular TNF (p<0.01). The percentage of T cells from PB an BM expressing cytoplasmic TNF was significantly higher in patients (PB:39.11±16.97%; BM:40.73±18.19%) than in normal controls (PB:15.74±7.95%; BM:18.80±12.93%) (p< 0.00001; p<0.005, respectively). In PB and BM from B-CLL patients the percentage of CD3+ cells expressing intracellular TNF was significantly higher than the percentage of CD19+/TNF+ cells (p<0.0001). Besides, it was found that the percentage of T cells expressing cytoplasmic TNF positively correlated with the stage of disease (p<0.01). In PB positive correlation were found between the number of T CD3+/TNF+ cells and lymphocytosis (p<0.05) and TTM (p<0.001). The percentage of leukaemic B cells positive for TNF did not correlate with the stage of disease. There was increased expression of TNF-RI and TNF-RII in leukaemic B cells in comparison to normal B-cells was observed (p<0.0001). We found positive correlation between the number of CD5+ B lymphocytes and the levels of soluble TNF-RII (sTNF-RII) (p< 0.05). The sTNF-RII levels in PB and BM significantly correlated with the stage of disease acc. Rai (p<0.0001). Furthermore, the sTNF-RII concentration positively correlated with lymphocytosis and TTM (p<0.0001). These results strongly support the key role TNF in B-CLL pathogenesis. Our results suggest that TNF may function as growth factor for B-CLL cells. CD3+T cells may be the important source of this cytokine in advanced B-CLL. It seems that changes in T cells capability to produce cytoplasmic TNF are associated with disease progression. However, further studies are required to confirm the key role of TNF in B-CLL pathogenesis.

The Immunoglobulin Gene Repertoire of Low-Count CLL-Like MBL Is Different from CLL: Diagnostic Considerations and Implications for Clinical Monitoring

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 779-779
Author(s):  
Antonis Dagklis ◽  
Claudia Fazi ◽  
Cinzia Sala ◽  
Valeria Cantarelli ◽  
Cristina Scielzo ◽  
...  
Keyword(s):  
B Cells ◽  
General Population ◽  
B Cell ◽  
B Lymphocytes ◽  
Low Frequency ◽  
Clinical Monitoring ◽  
Gene Repertoire ◽  
Healthy Individuals ◽  
Molecular Features ◽  
Ighv Gene

Abstract The term Monoclonal B Lymphocytosis (MBL) defines the presence of Monoclonal B Lymphocytes in the blood of otherwise healthy individuals. Though phenotypically heterogeneous, most MBL cases resemble CLL cells (CD5+, CD20dim, CD79b dim, sIgdim). The interest in MBL increased after this entity was included in the revised NCI-WG/IWCLL guidelines for the diagnosis and management of CLL (Hallek et al, 2008) and defined as “the presence of fewer than 5×109/L B lymphocytes” in the peripheral blood. However, the concentration of MBL in the blood of any given individual is extremely variable accounting in some cases for the vast majority of circulating B cells while being a negligible portion of them in others. It is then plausible that molecular differences could exist between low- vs. high-count MBL, being the latter likely more advanced on the way to become CLL. In this context, it was recently reported that subjects with <5×109/L CLL-like MBL but with lymphocytosis will require treatment at a rate of 1.1% per year. The absolute B-cell count turned out to be the only independent prognostic factor associated with progressive lymphocytosis, as all MBL cases studied had immunoglobulin (IG) gene features and cytogenetic abnormalities similar to good prognosis CLL. In contrast, very little is known about low-count MBL cases accidentally found in the general population. By cytofluorograph analysis, we identified 89 CLL-like MBL in the blood of 1725 healthy individuals >18 years old (5.1%) and analyzed the IGHV-D-J rearrangements expressed by 51 of them, the majority being characterized by few clonal B cells (mean 6.9% of circulating B lymphocytes, with only 13 cases >10%). CLL-like MBL cells showed a predominance of IGHV3 genes, followed by IGHV4 genes, resembling the normal repertoire. The most frequent IGHV gene in MBL was IGHV4- 59/61, rarely used in CLL. The MBL repertoire was also conspicuous for the lack of the IGHV1-69 gene (the predominant gene in unmutated CLL, ~25%) and the low frequency of the IGHV4-34 gene (2/51 sequences, 3.9%), the most frequent gene in mutated CLL (~12%). Following the 98% identity cut-off value, 36/51 sequences (70.5%) were defined as “mutated”, whereas the remainder had “unmutated” IGHV genes. Alignment of MBL HCDR3 sequences to a comprehensive panel of CLL HCDR3 sequences identified 2/51 (3.9%) MBL cases with a sequence similar to previously described CLL cases (“stereotyped receptors”). These results show that the IG gene repertoire in low-count MBL, accidentally found in the general population, does not show the typical CLL-related biases in terms of IGHV gene usage. This cannot be simply explained by the higher number of mutated cases among MBL, as unmutated cases account for almost a third of CLL-like MBL, indicating a molecular heterogeneity. In addition, HCDR3 stereotypy in MBL is significantly less frequent than in CLL (>25% of cases). Occasional MBL may indeed express a CLL “stereotyped receptor”, implying that the potential to evolve into a leukemia exists within MBL, though at low frequency, and may depend on precise selection mechanisms based on the molecular features of the B cell receptor. Taken together, our results strongly suggest that the detection of MBL in an otherwise healthy subject is not always equivalent to a pre-leukemic state. Differential IG molecular features might provide a better tool to discriminate individuals at risk of progression than an arbitrary mathematical threshold. Detailed IG molecular analysis of individual MBL may help to identify those few cases that necessitate continuous clinical monitoring to anticipate disease progression and, on the other hand, to avoid the burden of lengthy and expensive follow-ups in the vast number of persons who are extremely unlikely to develop CLL, though carrying MBL in their blood.

scholarly journals The bcl-2 gene product inhibits clonal deletion of self-reactive B lymphocytes in the periphery but not in the bone marrow.

1993 ◽  
Vol 178 (4) ◽  
pp. 1247-1254 ◽  
Author(s):  
S Nisitani ◽  
T Tsubata ◽  
M Murakami ◽  
M Okamoto ◽  
T Honjo
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Transgenic Mice ◽  
Gene Product ◽  
B Lymphocytes ◽  
Immunoglobulin Genes ◽  
Clonal Deletion ◽  
Immature B Cells ◽  
B1 Cells ◽  
Self Antigen

To test whether the product of the bcl-2 proto-oncogene blocks clonal deletion of self-reactive B cells, we have generated transgenic mice carrying the bcl-2 gene and the immunoglobulin genes for the anti-erythrocyte 4C8 antibody. In these transgenic mice, clonal deletion of self-reactive immature B cells in the bone marrow was not inhibited in spite of expression of the bcl-2 gene. In contrast, self-antigen-induced clonal deletion of mature self-reactive Ly-1 B (B1) cells in the peritoneal cavity was inhibited in the transgenic mice. These results indicate that the mechanism for clonal deletion of immature self-reactive B cells in the bone marrow differs from that of mature self-reactive B cells in the periphery.

scholarly journals BENEFITS OF VOLUMETRIC COMPRESSION OSCILLOMETRY FOR THE ASSESSMENT OF HEMODYNAMIC PARAMETERS IN PATIENTS WITH ARTERIAL HYPERTENSION

2013 ◽  
Vol 12 (2) ◽  
pp. 10-17
Author(s):  
L. V. Shpak ◽  
E. S. Galoshina
Keyword(s):  
Arterial Hypertension ◽  
Vascular Wall ◽  
Healthy People ◽  
Control Group ◽  
Hemodynamic Parameters ◽  
Simultaneous Reduction ◽  
Non Invasive ◽  
Wide Range ◽  
The Status ◽  
Stage 1

Aim. To compare the parameters of central and peripheral hemodynamics in healthy people and patients with Stage 1–3 arterial hypertension (AH).Material and methods. In total, 105 individuals were examined. The control group (CG) included 50 healthy people (25 women and 25 men; mean age 27,8±0,8 years) with optimal and normal levels of blood pressure (BP) (mean levels 118,5±1,6/71,82±1,2 mm Hg). The main group (MG) included 55 patients (41 women and 14 men; mean age 62,9±1,6 years) with systolo-diastolic AH: Stage 1 in 25 (mean BP levels 146,1±0,9/84,9±1,6 mm Hg), Stage 2 in 20 (164,4±1,8/95±2,1 mm Hg), and Stage 3 in 10 (189,6±10,6/92,6±6,3 mm Hg). The method of volumetric compression oscillometry (VCO) was used to assess a wide range of myocardial and hemodynamic parameters.Results. In AH patients, all AH phenotypes, vascular and cardiac parameters were increasing, with a simultaneous reduction in vascular wall distensibility, in parallel with the AH progression from Stage 1 to Stage 3. This indicated an increase in myocardial contractility, tone strain of arterial wall, and peripheral vascular resistance. From Stage 1 to Stage 3, the prevalence of hyper- and eukinetic cardiac hemodynamic types was decreasing, while the prevalence of mixed and hypokinetic types was increasing. The mixed hemodynamic type (a combination of hyper-, eu-, and particularly hypokinetic type characteristics) was considered as an incompletely developed disadaptive hypokinetic type.Conclusion. The VCO method is an effective, non-invasive way to simultaneously assess the status of multiple hemodynamic parameters in both healthy people and AH patients. A specific benefit of this method is the registration of lateral BP levels and identification of mixed (additional) hemodynamic type. 

scholarly journals Characterization of B1-cells during experimental leukomogenesis

2020 ◽  
Vol 65 (1) ◽  
pp. 35-40
Author(s):  
I. Yu. Ezdakova ◽  
O. V. Kapustina ◽  
M. I. Gulyukin ◽  
T. V. Stepanova
Keyword(s):  
Immune Response ◽  
B Cells ◽  
B Lymphocytes ◽  
Early Period ◽  
Primary Immune Response ◽  
Functional Difference ◽  
Infected Cattle ◽  
Primary Target ◽  
B1 Cells

Background. Bovine leukemia causes a significant polyclonal expansion of CD5+ , IgM+ B lymphocytes, known as persistent lymphocytosis (PL), in approximately 30% of infected cattle. However, it is not yet clear what happens to this subpopulation of B cells in the early period of infection of animals.Purpose. Quantitative characterization of IgM+ and CD5+ B cells during the immune response, which can provide important information on the mechanisms of lymphocyte priming in BLV infection.Material and methods. The experiment used BLV-negative calves of black-motley breed at the age of 8 months (n = 11). Animals (n = 8) were intravenously injected with blood of a BLV-positive cow. Control calves (n = 3) were injected with saline. Studies were performed before and after infection on days 5, 7, 14, 21, 28 and 65 of the immune response. The determination of the number of B-lymphocytes in the blood was carried out by the method of immunoperoxidase staining based on monoclonal antibodies to IgM, CD5.Results. As a result of the studies, it was found that the level of CD5+ B cells increases on the 14th day of the primary immune response, characterized by polyclonal proliferation of CD5+ B cells, which are the primary target for BLV. Our research data confirm that in the lymphocytes of experimentally infected cattle, surface aggregation of IgM and CD5 molecules on B-lymphocytes is absent.Discussion. It is known that the wave-like nature of IgM synthesis, which was shown in previous studies, depends on a subpopulation of B1 cells. After 7 days of the immune response, IgM+ and CD5+ cells do not correlate, which shows their functional difference. The increase in CD5+ cells is probably not associated with B cells, but with T cells differentiating under the influence of the virus.Conclusions. A subset of B1 cells is the primary target of cattle leukemia virus. The 65th day of the immune response is characterized by the expansion of IgM+ B cells, a decrease in the number of CD5+ cells and a uniform distribution of receptors around the perimeter of the cells.

Role of CD20 + IL-10 + B-lymphocytes in immunoregulatory processes in women with reccurent miscarriage

2021 ◽  
Vol 66 (8) ◽  
pp. 485-488
Author(s):  
A. V. Kust ◽  
N. Y. Sotnikova ◽  
A. I. Malyshkina ◽  
D. N. Voronin
Keyword(s):  
Pregnant Women ◽  
B Lymphocytes ◽  
Recurrent Miscarriage ◽  
Sharp Decrease ◽  
Main Group ◽  
Relative Content ◽  
Control Group ◽  
Becton Dickinson ◽  
Spontaneous Miscarriage ◽  
History Of

To determine the level of CD20 + IL-10 + B-lymphocytes in pregnant women with the threat of termination of pregnancy at 5-12 weeks and recurrent miscarriage in history and compare the data obtained with the end of gestation. A survey of 65 women at a gestational age of 5-12 weeks was carried out. The main group consisted of 33 women with a threatening recurrent miscarriage at the time of the examination, the comparison group consisted of 10 pre-pregnant women with a threatening sporadic miscarriage at the time of the examination, the control group consisted of 22 pregnant women without signs of a threatening miscarriage. The main group, depending on the outcomes of pregnancy, is divided into 2 subgroups: subgroup A - pregnancy ended in undeveloped pregnancy or miscarriage (9 women), subgroup B - pregnancy ended in childbirth (24 women). The relative content of CD20 + IL-10 + B-lymphocytes was determined by flow cytometry on FACSCanto II (Becton Dickinson, USA). Women in the main group had a significantly lower level of CD20 + IL-10 + B-lymphocytes in comparison with the rest of the surveyed. A retrospective analysis revealed that among women of subgroup A there was a sharp decrease in CD20 + IL-10 + cells compared with subgroup B. Prediction of a non-developing pregnancy and spontaneous miscarriage up to 22 weeks of gestation in pregnant women with threatened spontaneous miscarriage and a history of recurrent miscarriage is possible with the relative content of CD20 + IL-10 + equal to or less than 4.5% (sensitivity 100%, specificity 82.6%, accuracy 87.9%).

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