Interference by piperacillin/tazobactam in the measurement of creatinine with the Jaffe method and of total protein with the biuret method

2021 ◽  
Vol 23 (2) ◽  
pp. 141-143
Author(s):  
Goce Dimeski ◽  
◽  
Peter S Kruger ◽  
◽  
Keyword(s):  
Serum Creatinine ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Clinical Medicine ◽  
Clinical State ◽  
Blood Collection ◽  
Laboratory Studies ◽  
Abnormal Result ◽  
Biuret Method ◽  
Positive Results

Serum creatinine and total protein are routinely measured biochemical parameters used in clinical medicine. An abnormal result caused by interference with the assay does not accurately reflect a patient’s clinical state and therefore risks misleading clinicians. We report the case of a patient who had unexplainable high creatinine and total protein results. The blood collection was contaminated with intravenous fluid and the patient was receiving piperacillin/tazobactam. Additional laboratory studies demonstrated piperacillin/tazobactam was the cause of the false positive results and the elevation in both serum creatinine and protein level was dependent on the concentration of antibiotic present.

scholarly journals Colostral immunoglobulins absorption in Canchim and Nelore calves

2004 ◽  
Vol 33 (6) ◽  
pp. 1544-1547 ◽  
Author(s):  
Raul Machado Neto ◽  
Irineu Umberto Packer ◽  
Gladys Villas Boas do Prado ◽  
Rosana Bessi ◽  
Patricia Pauletti
Keyword(s):  
Specific Gravity ◽  
Statistical Model ◽  
Total Protein ◽  
Blood Circulation ◽  
Statistical Significance ◽  
Blood Collection ◽  
Endogenous Production ◽  
Effect Difference ◽  
Biuret Method ◽  
Newborn Calves

The efficiency of absorption of colostral immunoglobulins was evaluated in five Canchim and seven Nelore calves. They received colostrum pools with concentration of 70.20 ± 6.14 mg/mL through esofageal feeder at 2, 12, 24 and 36 hours after birth. The immunoglobulins concentrations of the pools were estimated through specific gravity and measured by radial immunodifusion. In the blood collection at birth and during the first 70 days of life, the total protein was assayed by biuret method and the immunoglobulins were assayed by radial immunodifusion. Data were analysed as a randomized split-plot statistical model. The highest concentrations of serum immunoglobulins and total protein were observed at 24 hours of age. No significant differences (P>0.5484) were observed for immunoglobulins concentration at 24 hours, with concentrations of 28.80 ± 7.24 mg/mL for Canchim and 27.32 ± 9.54 mg/mL for Nelore. The efficiency for immunoglobulins absorption was not significantly different (P>0.8715) between breeds, 64.04 ± 7.74% for Canchim and 62.30 ± 6.93% for Nelore. The lack of statistical significance persisted until the fourtieth day of life, period of maternal immunoglobulin predominance in the calves blood circulation. In the following period, from 40 to 70 days of age, phase of establishment of the endogenous production of immunoglobulin, differences in the IgG concentrations between the two groups were detected refflecting a possible breed effect difference. The process of colostral IgG absorption by the newborn calves was not affected by breed. The differences between breeds in the calves serum IgG were related to the phase of endogenous production of antibodies.

Haemato-Biochemical Alterations After Epidural Administration of Dexmedetomidine Alone and in Combination with Bupivacaine in Buffalo Calves

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
A. Jaiswal ◽  
S. S. Pandey ◽  
A. S. Parihar ◽  
N. Rajput ◽  
R. Jain
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Glucose ◽  
Serum Creatinine ◽  
Total Protein ◽  
Epidural Injection ◽  
Epidural Administration ◽  
Healthy Male ◽  
Buffalo Calves ◽  
Biochemical Alterations ◽  
Biochemical Examination

The study was conducted on 12 healthy male buffalo calves weighing between 50 to 60 Kg to evaluate haemato-biochemical alterations after epidural injection of dexmedetomidine alone and in combination with bupivacaine. Haematological examination showed nonsignificant alterations. Biochemical examination showed significant increase in blood glucose, blood urea nitrogen and serum creatinine while there was nonsignificant alterations in total protein and alkaline phosphatase. All haemato-biochemical changes were transient and compensatory.

Assessment of some hematological indicators of breeding falls of the zabaikalsky breed

2021 ◽  
Vol 1 (2) ◽  
pp. 36-42
Author(s):  
B. Z. Bazaron ◽  
◽  
T. N. Khamiruev ◽  
S. M. Dashinimaev ◽  
G. M. Shkuratova ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Steppe Zone ◽  
Diagnostic Methods ◽  
Clinical State ◽  
Forest Steppe ◽  
Research Material ◽  
Hematological Indicators ◽  
Young Horses

The results of studying the morphological and biochemical parameters of the blood of young horses of the Trans-Baikal breed, depending on the zone of their breeding and age, are presented. The study of hematological parameters is one of the most important diagnostic methods for assessing the physiological and clinical state of animals. The research material was stallions at the age of 12, 18, 24 and 36 months. It was found that hematological parameters were within physiological norms, while in stallions of the forest-steppe zone they were higher in comparison with analogs of the steppe zone. With age, there is a decrease in the estimated hematological parameters in the experimental stallions.

scholarly journals Effects of peptidic growth hormone secretagogue receptor (GHS-R) antagonist [D-Lys3] on some of serum hormonal and biochemical parameters in Wistar rat model

2014 ◽  
Vol 58 (3) ◽  
pp. 288-291 ◽  
Author(s):  
Habib Aghdam Shahryar ◽  
Alireza Lotfi
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Serum Lipids ◽  
Considerable Increase ◽  
Wistar Rat ◽  
Male Rats ◽  
Growth Hormone Secretagogue Receptor ◽  
Growth Hormone Secretagogue ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective : The present study investigated the effects of different dosages of a GHS-R antagonist [D-Lys3] on some serum hormonal (cortisol, T3 and T4) and biochemical parameters in a rat.Materials and methods : Thirty-six 60-day-old male rats were assigned to four treatments. [D-Lys3]-GHRP-6 solutions were infused via intraperitoneal injections. Blood was collected and analyzed.Results : The large dosages of a GHS-R antagonist (200 ng/kg BW) caused increases in cortisol, whereas no significant changes occurred when low dosages were injected. There were no significant changes in T3 and T4 following the administration of the GHS-R antagonist, but a considerable increase was observed in blood glucose levels of the groups (G50, G100, and G200 ng/kg BW). There was a significant increase in total protein when the greatest dose was administrated (G200 ng/kg BW). However, total cholesterol, triglycerides, and albumin showed no significant changes.Conclusions : Exogenous GHS-R antagonist can cause an increase in glucose and moderate increases in cortisol and total protein, yet it has no significant effect on T3 and T4 levels or on the concentrations of serum lipids. The effect of GHS-R antagonist is not completely adverse to the effects of ghrelin. Further molecular studies are necessary to identify the physiological effects of the peptidic GHS-R antagonist. Arq Bras Endocrinol Metab. 2014;58(3):288-91

scholarly journals Serum biochemical properties of Vietnamese indigenous Noi chicken at 56 days old

2020 ◽  
Vol 36 (3) ◽  
pp. 329-339
Author(s):  
Nguyen Giang ◽  
Nguyen Hien ◽  
Huynh Loan ◽  
Phan Phuc ◽  
Nguyen Dai ◽  
...  
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Animal Health ◽  
Correlation Coefficients ◽  
Biochemical Properties ◽  
Blood Samples ◽  
Male And Female ◽  
Native Breed ◽  
Serum Biochemical ◽  
The Relationship

Serum profile is generally considered as comprehensive data which directly reflected animal health and their potential resistance to environmental, nutritional and pathological stress. The present study aimed to provide physiological reference values for selected biochemical parameters in Noi chickens, a famous Vietnamese native breed, at 56 days old. The collected blood samples of 355 Noi birds (164 males and 191 females) were used to evaluate biochemical serum profile. Glucose, total protein, albumin, globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine, and uric acid in the serum were measured. The variation in these values arising from different sexes was also investigated. The results indicated that there were no differences (P>0.05) in the assessed indices between male and female Noi chickens. In overall for the whole group, these values were obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The relationship among the biochemical parameters showed relatively low coefficients (r=0.79 in maximum). The females obtained higher correlation coefficients between levels of total protein and glucose (r=0.42) as well as between total protein and globulin (r=0.79), compared to the males. It was concluded that the male and female chickens performed similar values of serum indices. The contribution of this study might expand the knowledge on the biochemical profile and improved breeding strategies of Vietnamese indigenous Noi chickens.

scholarly journals Biochemical changes in blood serum of rats with experimental burn disease and their correction with melanin

2016 ◽  
Vol 21 (2) ◽  
pp. 44-48
Author(s):  
N. Chornenka ◽  
Ya. Rayetska ◽  
O. Savchuk ◽  
L. Ostapchenko
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Electrolyte Balance ◽  
Experimental Burn ◽  
Metabolic Systems ◽  
Immature Rats ◽  
Urea Metabolism ◽  
Activity Of Enzymes ◽  
Burn Disease

During modeling of esophageal alkali burns 1 and 2 degrees in immature rats were observed violations of various metabolic systems, melanin administration helped to normalize the biochemical parameters. It was shown that under the esophageal burns there occurred violations of protein (total protein, albumin), nitrogen metabolism (creatinine, urea) metabolism of carbohydrate (glucose) and lipids (cholesterol), changed the activity of enzymes (GOT, GPT, CC), violated fluid and electrolyte balance (K +, Na +, Cl-, Ca2 +, Mg2 +, phosphates), while the introduction of melanin contributed to the normalization of these indicators. The most effective dose were of 0.5 mg/ kg and 1 mg/kg. There were found anti-toxic properties of melanin, which may be a promising drug for the normalization of metabolism in patients with burns of esophagus and prevent its complications.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals EXERCISE-INDUCED CHANGES IN THE LEVEL OF TOTAL PROTEIN AND ITS FRACTIONS IN THE BLOOD OF HORSES INVOLVED IN RECREATIONAL HORSEBACK RIDING

2020 ◽  
pp. 35-46
Author(s):  
Halyna Tkachenko ◽  
Natalia Kurhaluk ◽  
Irina Tkachova
Keyword(s):  
Total Protein ◽  
Colorimetric Method ◽  
Exercise Session ◽  
Rank Test ◽  
Horseback Riding ◽  
Jugular Veins ◽  
Blood Samples ◽  
Biuret Method ◽  
G Ratio ◽  
Resting Period

The aim of the current study was to do the analysis of the total protein and its fraction in the blood samples of horses, which are involved in recreational horseback riding in the Pomeranian region (Pomeranian Voivodship, northern Poland). Thirteen healthy adult horses from the Pomeranian region in Poland (Strzelinko village, N54°30´48.0´´ E16°57´44.9´´), aged 9.5±2.4 years, including 5 Hucul ponies, 2 Thoroughbred horses, 2 Anglo-Arabian horses, and 4 horses of unknown breed, were used in the current study.Training started at 10:00 AM, lasted 1 hour, and consisted of a ride of cross country by the walking (5 min), the trotting (15 min), the walking (10 min), the trotting (10 min), the walking (5 min), the galloping (5 min), and the walking (10 min). Blood samples were taken from the jugular veins of the animals in the morning time, 90 minutes after feeding, while the horses were in the stables (between 8:30 and 10 AM), and immediately after the exercise session (between 11:00 AM and 2:00 PM). To obtain serum, the blood was collected in plain tubes without anticoagulants. Blood was stored in tubes with K3-EDTA and held on ice until centrifugation at 3,000g for 15 minutes. The plasma was removed.The total protein and its fractions were measured at +23°C by the biuret method with the use of commercially available reagents and a compact semi-automated analyzer RX Monza (Randox Laboratories LTD., UK) according to the procedures described by the manufacturer. The biuret method is the most widely used colorimetric method for the determination of the total protein concentration in serum because of its simplicity, precision, and accuracy. The absorbance of each sample was measured in duplicate.Results are expressed as mean ± S.E.M. All variables were tested for normal distribution using the Kholmogorov-Smirnov test (p>0.05). To find significant differences (significance level, p<0.05) between at the rest and after exercise, the Wilconson signed-rank test was applied to the data. All statistical analyses were performed using STATISTICA 8.0 software (StatSoft, Krakow, Poland). The total protein level in the blood of horses exhibited a non-significant increase (by 7.1%, p>0.05) immediately after exercise as compared to the resting period. Also, the albumin and globulin levels in the blood of horses were non-significantly increased by 5.9% (р>0.05) and 8.1% (р>0.05) after the training sessions. There were no significant differences in serum albumin/globulin ratio between the resting period and after exercise (0.997±0.09 vs. 0.977±0.08). The results of our current study showed that exercise has a statistically non-significant effect on the total proteins and their fractions in equine serum. The fractions and the A/G ratio were within the range of values obtained in horses in other studies. Thus, it was found that total protein and its fractions were increased in horses after training, and this increase was insignificant. This increase has a direct correlation with exercise. In this paper, it is shown that training can change the physiology and affect the biochemistry of hematobiochemical blood parameters in horses subjected to physical exertion.

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