Transmission of the effect of Mercurius corrosivus 30 CH on -amylase in cell free medium through water.

2021 ◽  
Vol 13 (47) ◽  
pp. 117-118
Author(s):  
Tandra Sarkar ◽  
Atheni Konar ◽  
Indrani Chakraborty ◽  
Nirmal Chandra Sukul ◽  
Anirban Sukul ◽  
...  

Abstract Background Mercurius corrosivus 30CH promoted -amylase activity in a cell free medium invitro. -amylase causes hydrolysis of starch. The activity of the enzyme is measured in terms of the amount of maltose liberated due to breakdown of starch. In a number of experimental studies it has been demonstrated that the effect of homeopathic potency would be transmitted from one plant to another through water. Here one leaf of a pair of plants was dipped in water in a beaker. The two beakers were connected by a water filled polythene tube. The effect of treatment of one plant with homeopathic potency would be observed in the directly treated plant as well as the connected plant. Two groups of toads were kept in water in two different containers. The two containers were connected by a water filled polythene tube. The effect of treatment of one group of toads with homeopathic potency would be observed in both the directly treated group as well as the connected group. Objectives The purpose of the present study is to see whether the effect of Mercurius corrosivus 30 CH on -amylase in one test tube would be transmitted to another test tube connected with the former by water filled capillary tube. Methods Mercurius corrosivus 30 CH was diluted with distilled water (1:100). Two hard glass test tubes each containing -amylase were connected with a water filled capillary tube while one test tube received Mercurius corrosivus 30CH solution, the other only the control solution. The control solution consisted of equal amount of 90% ethanol diluted with water (1:100).There were two more test tubes, one containing same amount of distilled water instead of Mercurius corrosivus 30 CH solution and the other test tube the same amount of 90% ethanol (1:100) as in the control set. After 10 mins starch solution in Sodium acetate buffer was added to each test tube. The enzyme in each test tube was allowed to react with starch for 15 mins and then it was stopped by DNSA (Dinitro salicylic acid) solution .The activity of -amylase was measured by standard biochemical process. The breakdown product maltose in each test tube was quantified by a standard curve prepared by measuring the optical density of the maltose solution at 560 nm in a UV-VIS Spectrophotometer. This experiment was repeated 20 times. Results Activity of -amylase was expressed in terms of the amount of maltose liberated from breakdown of starch with standard errors in 15mins at a fixed temperature. The data were analyzed statistically using t-test. Mercurius corrosivus 30 CH enhanced the enzyme activity significantly in the directly treated test tube as well as the connected one (p

1953 ◽  
Vol 51 (2) ◽  
pp. 203-214 ◽  
Author(s):  
E. J. L. Lowbury ◽  
Jean Fox

Suspensions ofStrep. pyogenes, Staph. aureus, Ps. pyocyaneaand micrococci in serum, distilled water, saline and oleic acid solution were allowed to dry on cover-slips. The numbers of bacteria surviving after periods of exposure were estimated from viable counts of extracted cover-slips.All the organisms tested showed after drying a fall in viable count that was absent in aqueous suspensions not allowed to dry.Ps. pyocyaneawas shown to have a lower survival rate on drying than the other organisms.Serum afforded moderate protection to the suspended organisms during the period of drying.0·1 mg./ml. oleic acid caused a rapid destruction ofStrep. pyogenesand a significant destruction ofPs. pyocyaneaas well as ofStaph. aureusand micrococci in drying films of these organisms. The similarity of the fate ofStaph. aureusand micrococci during drying in low concentrations of oleic acid is in contrast to the previously reported greater sensitivity ofStaph. aureusto higher concentrations of oleic acid (1–10 mg./ml.) in the test-tube.The implications of these findings are discussed in relation to skin and wound flora, and to environmental hygiene.


Author(s):  
Ina Grau ◽  
Jörg Doll

Abstract. Employing one correlational and two experimental studies, this paper examines the influence of attachment styles (secure, anxious, avoidant) on a person’s experience of equity in intimate relationships. While one experimental study employed a priming technique to stimulate the different attachment styles, the other involved vignettes describing fictitious characters with typical attachment styles. As the specific hypotheses about the single equity components have been developed on the basis of the attachment theory, the equity ratio itself and the four equity components (own outcome, own input, partner’s outcome, partner’s input) are analyzed as dependent variables. While partners with a secure attachment style tend to describe their relationship as equitable (i.e., they give and take extensively), partners who feel anxious about their relationship generally see themselves as being in an inequitable, disadvantaged position (i.e., they receive little from their partner). The hypothesis that avoidant partners would feel advantaged as they were less committed was only supported by the correlational study. Against expectations, the results of both experiments indicate that avoidant partners generally see themselves (or see avoidant vignettes) as being treated equitably, but that there is less emotional exchange than is the case with secure partners. Avoidant partners give and take less than secure ones.


Author(s):  
Dmitry A. Neganov ◽  
◽  
Victor M. Varshitsky ◽  
Andrey A. Belkin ◽  
◽  
...  

The article contains the comparative results of the experimental and calculated research of the strength of a pipeline with such defects as “metal loss” and “dent with groove”. Two coils with diameter of 820 mm and the thickness of 9 mm of 19G steel were used for full-scale pipe sample production. One of the coils was intentionally damaged by machining, which resulted in “metal loss” defect, the other one was dented (by press machine) and got groove mark (by chisel). The testing of pipe samples was performed by applying static internal pressure to the moment of collapse. The calculation of deterioration pressure was carried out with the use of national and foreign methodical approaches. The calculated values of collapsing pressure for the pipe with loss of metal mainly coincided with the calculation experiment results based on Russian method and ASME B31G. In case of pipe with dent and groove the calculated value of collapsing pressure demonstrated greater coincidence with Russian method and to a lesser extent with API 579/ASME FFS-1. In whole, all calculation methods demonstrate sufficient stability of results, which provides reliable operation of pipelines with defects.


Author(s):  
M. A. Danilov ◽  
◽  
M. V. Drobysh ◽  
A. N. Dubovitsky ◽  
F. G. Markov ◽  
...  

Restrictions of emissions for civil aircraft engines, on the one hand, and the need in increasing the engine efficiency, on the other hand, cause difficulties during development of low-emission combustors for such engines.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cicero Antônio Mariano dos Santos ◽  
Joacir do Nascimento ◽  
Kelly Cristina Gonçalves ◽  
Giovani Smaniotto ◽  
Leonardo de Freitas Zechin ◽  
...  

AbstractSpodoptera frugiperda is a pest of economic importance for several crops with resistance reports to Bt crops and pesticides. Eco-friendly Bt biopesticides may be an alternative to chemical insecticides due to their selectivity and specificity. However, the efficacy of Bt biopesticides may be influenced by the association with other chemicals, such as adjuvants. This study evaluated the compatibility and toxicity of Bt biopesticides mixed with adjuvants for the control of S. frugiperda. The treatments included the association of Dipel SC and Dipel PM with adjuvants. Compatibility tests were used to evaluate the Bt mixture. Bt suspensions obtained from mixtures of Bt and adjuvants at 106 and 3 × 108 spores/mL−1 were used to evaluate S. frugiperda mortality and distilled water was used as the control. The addition of the adjuvant LI increased growth and sporulation, indicating compatibility with Bt biopesticides. The other adjuvants were toxic to reducing Bt growth and sporulation. Only the mixture of Bt with LI and Bt alone was effective to S. frugiperda. The addition of adjuvants to Bt biopesticide affect the Bt sporulation, growth and mortality.


2021 ◽  
Vol 45 (2) ◽  
pp. 186-196
Author(s):  
Paulina Szwed ◽  
Małgorzata Kossowska ◽  
Marcin Bukowski

AbstractAccording to the principle of energy-conservation principle, effort investment is usually reduced in situations that are perceived as uncontrollable. This is because when success is recognized as impossible, any effortful actions are no longer justified. However, we predicted that individual differences in uncertainty tolerance, i.e., the need for closure (NFC), may moderate effort investment in uncontrollable situations. We tested this prediction in two experimental studies in which we exposed participants with differing levels of NFC to uncontrollable events, and indexed effort through the assessment of systolic blood pressure (SBP) responses. As predicted, in the uncontrollability (vs. controllability) condition, effort investment decreased significantly among low- but not high-NFC participants. Since gaining certainty and achieving closure is not a critical epistemic goal for low-NFC individuals, exerting extra effort to gain certainty is therefore no longer justified. On the other hand, high-NFC participants do not withhold their efforts, as they are highly motivated to obtain certainty. These results may help to account for contradictory findings in effort-investment behaviour and add substantively to the literature concerning motivation toward closure.


1925 ◽  
Vol 9 (2) ◽  
pp. 123-136
Author(s):  
Howard J. Shaughnessy ◽  
Katharine I. Criswell

1. The strain of Bacterium coli used in these experiments multiplies in distilled water at pH 6.0 and pH 8.0 and in Ringer-Locke solution at pH 6.0. Under all the other conditions studied the numbers decrease with the passage of time. 2. The electrophoretic charge of the cells is highest in distilled water at pH 6.0 and pH 8.0. Under all other conditions studied the velocity of migration is decreased, but the decrease is immediate and is not affected by more prolonged exposure. 3. A strongly acid solution (pH 2.0) causes a rapid death of the cells and a sharp decrease in electrophoretic charge, sometimes leading to complete reversal. 4. A strongly alkaline solution (pH 11.0) is almost as toxic as a strongly acid one, although in distilled water the organisms survive fairly well at this reaction. Electrophoretic charge, on the other hand, is only slightly reduced in such an alkaline medium. 5. In distilled water, reactions near the neutral point are about equally favorable to both viability and electrophoretic charge, pH 8.0 showing slightly greater multiplication and a slightly higher charge than pH 11.0. In the presence of salts, however, pH 8.0 is much less favorable to viability and somewhat more favorable to electrophoretic charge than is pH 6.0. 6. Sodium chloride solutions, in the concentrations studied, all proved somewhat toxic and all tended to depress electrophoretic charge. Very marked toxicity was, however, exhibited only in a concentration of .725 M strength or over and at pH 8.0, while electrophoretic migration velocity was only slightly decreased at a concentration of .0145 M strength. 7. Calcium chloride was more toxic than NaCl, showing very marked effects in .145 M strength at pH 8.0 and in 1.45 M strength at pH 6.0. It greatly depressed electrophoretic charge even in .0145 M concentration. 8. Ringer-Locke solution proved markedly stimulating to the growth of the bacteria at pH 6.0 while at pH 8.0 it was somewhat toxic, though less so than the solutions of pure salts. It depressed migration velocity at all pH values, being more effective than NaCl in this respect, but less effective than CaCl2. 9. It would appear from these experiments that a balanced salt solution (Ringer-Locke's) may be distinctly favorable to bacterial viability in water at an optimum reaction while distinctly unfavorable in a slightly more alkaline solution. 10. Finally, while there is a certain parallelism between the influence of electrolytes upon viability and upon electrophoretic charge, the parallelism is not a close one and the two effects seem on the whole to follow entirely different laws.


Author(s):  
N.B. Afonina ◽  
A.V. Otrokov ◽  
G.Sh. Khazanovich

Most modern roadheaders of selective action are equipped with loading devices with gathering-stars, which have a simpler drive design and increased reliability. As a result of the experimental studies carried out at SRSPU (NPI), it was found that under certain conditions, it is possible to transfer large lump material of a pile into a fluidized state, which reduces the energy consumption of material loading. An increase in the number of beams of the gathering-stars reduces the influence of the other parameters of the loader. Analysis of the process of interaction of the gathering-star’s beam with the receiving conveyor’s side edge to avoid jamming of the picking gathering-star by a lump of material with the implementation of high dynamic loads, it is necessary to set the gathering-beams at an angle of at least 60 degrees against rotation.


1971 ◽  
Vol 14 (2) ◽  
pp. 262-270 ◽  
Author(s):  
S. D. G. Stephens ◽  
C. M. B. Anderson

A number of experimental determinations of the uncomfortable loudness level (ULL) at 1000 Hz were made on several groups of normal-hearing subjects, using various methods of stimulus presentation and applying different personality measures to the subjects. The same mean levels were found for both earphone and free-field presentations. In experienced subjects the monaural-binaural difference was between 2.5 and 4 dB in different experiments. In naive subjects this difference was 6 dB. In two groups of subjects, ULL was found to be significantly negatively correlated with their test anxiety scores, but this correlation did not hold for the other two groups tested. Naive subjects showed little difference in intersubject variance with the manual or Bekesy presentation techniques.


Author(s):  
João Felipe Besegato ◽  
Gabriela Dos Santos Ribeiro Rocha ◽  
Marlene De Sousa Amorim ◽  
Fabio Martins Salomão ◽  
Daniel Poletto ◽  
...  

Objective: to measure pH values of bleaching agents that are indicated to intracoronal bleaching technique in different time intervals. Methods: Each group (G) received five samples (n=5): G1 – distilled water (AD); G2 – hydrogen peroxide (H2O2) 30%; G3 – sodium perborate (PbS) + AD; G4 – PbS + H2O2 30%; G5 – sodium percarbonate (PcS) + AD; and G6 – PcS + H2O2 30%. pH values were stated using a digital pHmeter, in different time intervals: immediately after handling (T0), 24 hours (T1) and 168 hours after handling (T2). The results were submitted to statistical analysis through Kruskal-Wallis and Mann Whitney tests, in this order, allowing multiple comparisons among the groups. To verify the effect of time in each group, Friedman test was applied. Results: In the evaluation of the effect of time in each group, it was observed that G2 presented acid behavior, while the other groups exhibited values close to neutrality or alkaline. Conclusions: H2O2 30% was the only agent that showed acidic behavior in every evaluation time. Meanwhile, PcS + H2O had the highest pH values.


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