scholarly journals Germination and vigor of lettuce seeds (Lactuca sativa L.) pelleted with homeopathic preparations Alumina and Calcarea carbonica subjected to toxic levels of aluminum.

2021 ◽  
Vol 9 (33) ◽  
pp. 138-146
Author(s):  
Filipe Pereira Giardini Bonfim ◽  
Rosana Gonçalves Rodrigues das Dores ◽  
Ernane Ronie Martins ◽  
Vicente Wagner Dias Casali

Background: aluminum toxicity is the most important factor limiting the growth of plants in acid soils, whereas current treatments are unfeasible. For this reason, alternatives are sought for, among which homeopathic treatment. Aims: this study aimed at evaluating the influence of homeopathic preparations Alumina 6cH, Alumina 12cH, Calcarea carbonica 6cH and Calcarea carbonica 12cH on the germination and vigor of lettuce seeds subjected to toxic levels of aluminum in paper-solution. At the same time, it was sought to develop a new procedure to apply homeopathic preparations in plants (pelleting). Methods: the statistical design was entirely randomized (CRD) with 6 treatments and 4 repetitions. Treatments included: 1) pelleted seeds/talc + Alum 6 cH; 2) pelleted seeds/talc + Alum 12cH; 3) pelleted seed/talc + Calc 6cH; 4) pelleted seeds/talc + Calc 12cH; 5) pelleted seeds/talc + distilled water; 6) non pelleted seeds (control). Variables evaluated were: germination percentage (GP), germination speed index (GSI) and radicle length (RL). Results: there was significant difference in GSI and RL – variables that reflect the vigor of seeds - between the samples treated with homeopathic preparations and the controls Conclusions: homeopathic preparations Alumina 6cH and 12cH and Calcarea carbonica 6cH and 12cH had significant effect on the vigor of lettuce seeds subjected to stress conditions.

2018 ◽  
Vol 11 (1) ◽  
pp. 382 ◽  
Author(s):  
Márcia Cunegundes da Silva ◽  
Emmanoella Costa Guaraná Araujo ◽  
Thiago Cardoso Silva ◽  
Adão Batista de Araújo ◽  
Tarcila Rosa da Silva Lins ◽  
...  

This study aimed to evaluate the allelopathic effects of aqueous extracts of Tectona grandis L.F. in various concentrations produced from leaves and flowers. Seeds of lettuce (Lactuca sativa L.) and germitest paper as substrate were used, the germitest paper was moistened with distilled water, and aqueous extract in concentrations of 100%, 75%, 50%, 25% and 0% (distilled water). The extracts were homogeneously deposited on twenty-five seeds evenly distributed in gerbox-type boxes. The materials used in the experiment were previously sterilized with alcohol (70%). A completely randomized experimental design with four replicates was used and the experiment was conducted under natural conditions of temperature and humidity. The tests were carried out in the laboratory, the experiment was watched for 10 days always at the same hour and the variables analyzed were Germination Percentage (%G), Speed of Germination Index (SGI), Mean germination time (MGT) and Mean speed of germination (MSG). The data were submitted to Analysis of Variance and compared by Tukey test considering 95% of probability. It was observed that there were no significant differences in leaf and flower extracts for the variables %G, MGT and MSG, however, in SGI aqueous leaf extract differed significantly at the highest concentrations (75% and 100%). It was concluded that aqueous extracts of leaves and flowers of T. grandis had no inhibitory potential in relation to the percentage and average germination time of lettuce seeds at any of the concentrations.


2019 ◽  
Vol 11 (11) ◽  
pp. 295
Author(s):  
Ricardo A. Felito ◽  
Oscar M. Yamashita ◽  
Wagner Gervazio ◽  
Marco A. C. Carvalho ◽  
Delmonte Roboredo ◽  
...  

The aim of this study was to evaluate the effect of homeopathic medicines in the neutralization of waste herbicide 2,4-D+picloram in cucumber seeds, in the municipality of Alta Floresta-MT process. The experiment was carried out to evaluate two herbicide concentrations and five Nux vomica dynamizations (6CH, 12CH, 18CH, 24CH and 30CH), in addition to two controls, one with distilled water and the other contaminated with herbicide alone. The variables assessed were germination percentage, germination speed index, fresh weight, dry weight, shoot length, root length and stem thickness, where we observed a significant difference in most of the variables analyzed. From the results, it can be concluded that the homeopathic preparations in five dynamics of Nux vomica (6CH, 12CH, 18CH, 24CH and 30CH), act positively on vigor and development of cucumber seeds.


2011 ◽  
Vol 13 (4) ◽  
pp. 507-511 ◽  
Author(s):  
J.M. Vasconcelos ◽  
M.A. Rodrigues ◽  
S.C. Vasconcelos Filho ◽  
J.F. Sales ◽  
F.G. Silva ◽  
...  

"Quina" (Strychnos pseudoquina A. St. Hil) is a medicinal plant species from the Brazilian Cerrado. As its seeds show dormancy, they were subjected to the treatments pre-cooling at 5ºC during 7 days, pre-heating at 40ºC during 7 days, pre-soaking in sulfuric acid PA during 5 and 15 min, pre-soaking in boiling water during 5 and 15 min, pre-soaking in 100 and 200 ppm gibberellic acid during 48 h, pre-soaking in distilled water during 24 and 48 h, and mechanical scarification to break dormancy. Counts were daily conducted from the 2nd day after the experiment implementation until the germination stabilization at the 65th day. The germination speed index (GSI) and the germination percentage were evaluated. Germination rates above 96% were reached in seeds pre-soaked in water during 48 h and substrate moistened with water or KNO3.


2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.


Author(s):  
Heider Alves Franco ◽  
Monica Regina da Costa Marques ◽  
Clarisse Ferreira Braga ◽  
Allana De Souza Izidorio ◽  
Sérgio Thode Filho

2021 ◽  
Vol 10 (2) ◽  
pp. e37110212551
Author(s):  
Larissa Éllen Coelho ◽  
Silvana Maria de Oliveira ◽  
Luiz Antonio de Souza ◽  
Lindamir Hernandez Pastorini

Allelopathy is analyzed as an alternative to herbicides due to the inhibitory or beneficent activities of its compounds with other organisms. Current paper discusses the effects of Aeschynomene fluminensis Vell. fractions on cultivated plant species, Lactuca sativa L. and Glycine max (L.) Merril, and on weeds, Ipomoea grandifolia (Dammer) O’Donnel and Digitaria insularis (L.) Fedde. Aqueous fractions at 0.80; 0.40; 0.20 and 0.10 mg mL-1 concentrations were employed for initial growth tests. Seeds were pre-germinated in distilled water and transferred to petri plates with separate fractions at different concentrations. Plates were maintained for 48 h in a germination chamber at 25°C for L. sativa and I. grandifolia and at 30°C for G.max and D. insularis. The length of hypocotyl (LH) and root (LR) was measured and LR and foliar length (LF) were taken for D. insularis seedlings. Parameters were employed to calculated inhibition percentage. Plants with morphological changes were fixed and analyzed anatomically. Results revealed LH and LR inhibition of lettuce seedlings in fractions with highest concentration rates. The same has been reported in the case of I. grandifolia. Butanolic, methanolic and chloroform fractions did not affect negatively soybean seedlings but they inhibited D. insularis seedlings´ LR. A. fluminensis fractions, especially at higher concentrations, inhibited seedlings´ growth and confirmed their phytotoxic capacity.


Irriga ◽  
2004 ◽  
Vol 9 (3) ◽  
pp. 207-216
Author(s):  
Guilherme Augusto Biscaro ◽  
Rodrigo De Menezes Trigueiro ◽  
Raimundo Leite Cruz ◽  
Marcelo Domingos Chamma Lopes

GERMINATION AND DEVELOPMENT OF AMERICAN LETTUCE SEEDLINGS (Lactuca sativa L.) IRRIGATED  WITH HOME AND INDUSTRIAL EFFLUENT-RECEIVING WATER   Guilherme Augusto Biscaro1; Rodrigo de Menezes Trigueiro2; Raimundo Leite Cruz2; Marcelo Domingos Chamma Lopes2State University of Mato Grosso do Sul, Cassilândia, MS, (Brazil), [email protected] Engineering Department, Agronomic Science College,Paulista State University ,  Botucatu-SP (Brazil).   1 ABSTRACT  This work has evaluated germination and formation of American lettuce seedlings (Lactuca sativa L.) “Tainá” variety, irrigated with urban effluent-receiving water from Lavapés Creek,  a water body which receives all home and industrial sewage of the city of  Botucatu, SP (Brazil). The sowing was carried out in 12 plastic foam trays with 32 germination cells each. At 41 days after planting (41-DAP), the seed germination percentage, seedling final size, final number of leaves, the mass of shoot and root dry matter were evaluated. Also, two sorts of substrates were investigated: Multiplantâ and Plug Mixâ. No complementary fertilization was done, so the only available nutrients were the ones  originally present in the substrates and in the creek water. For compararison an evaluation was performed using a source of treated water instead of the creek water. Results pointed out that all sown seeds presented 100% of germination. Treatments with  Multiplantâ presented the greatest values for plant height. Treatments irrigated with Lavapés  Creek water presented a greater number of leaves at 41-DAP.  KEYWORDS: Seedling germination, Lactuca sativa L., effluent receiving water.   BISCARO, G. A.; TRIGUEIRO, R. M.; CRUZ, R. L.; LOPES, M. D. C. GERMINAÇÃO E DESENVOLVIMENTO DE MUDAS DE ALFACE AMERICANA (Lactuca sativa L.) IRRIGADOS COM ÁGUAS RECEPTORAS DE EFLUENTES DOMÉSTICOS E INDUSTRIAIS   2 RESUMO  O trabalho avaliou a germinação e a formação de mudas de alface americana (Lactuca sativa L.) variedade “Tainá”, irrigada com águas receptoras de efluentes urbanos do Ribeirão Lavapés, curso d’água que recebe todo o esgoto doméstico e industrial da cidade de Botucatu – SP (Brasil). A semeadura foi realizada em 12 bandejas de isopor com 32 células de germinação cada. Foram analisados com 41 dias após o plantio (41-DAP), a porcentagem de germinação das sementes, o tamanho final das mudas, o número final de folhas, a massa de matéria seca da parte aérea e de raízes. Também foram estudados dois tipos de substrato: Multiplantâ e Plug Mixâ. Não foi utilizada nenhuma adubação complementar, estando disponível apenas os nutrientes presentes originalmente nos substratos e na água do ribeirão. Para efeito comparativo, realizou-se a mesma avaliação utilizando uma fonte de água tratada. Os resultados indicaram que todas as células semeadas apresentaram 100% de germinação. Os tratamentos que utilizaram o Multiplantâ apresentaram maiores valores de altura de plantas e os irrigados com a água do Ribeirão Lavapés, apresentaram maior número de folhas com 41-DAP.  UNITERMOS: Germinação de mudas, Lactuca sativa L., águas receptoras de efluentes.


Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Nina C Barboza Da Silva ◽  
Carla Holandino

Lettuce seeds bioassays have been used in many different tests such as: alellopathyc models; developing of new drugs; ecotoxicity tests. In most cases, lettuce (Lactuca sativa L., Asteraceae) has been used because of its sensitivity, simultaneous and rapid germination, reliability of germination percentage and homogeneity of seeds. The main goal was to evaluate the effects of ultra-high diluted gibberellic acid (GA3) on lettuce seeds germination and seedling growth. Experiment was performed using Petri dishes containing one disk of Whatman nº01 paper watered with 1ml of water. In each Petri dish 10 lettuce seeds(Lactuca sativa L.) cv Regina 500 were placed and 2ml of the different treatment solutions were add: GA33µmol, GA3 3CH (10-6), GA3 12CH (10-24), water 12CH and water (no dilution and succussion). One milliliter solutions were added every 2 days of experiment. The experiment was repeated twice and each one consisted in 5 Petri dishes per treatment (n=100). All seeds were maintained in germination incubator under controlled temperature (25°C) and photoperiod (16L/8D). The tested substances were prepared according to Brazilian Homeopathic Pharmacopoeia (Brazil, 2011). The experiment was blinded all the time. All seeds germinated at same time (2 days) and after 7 days the germination rate was the same in all treatments. Root was affected just by Water 12 CH, in which shown the longest length (4.59 cm) when compared with others treatments. Shoot length was higher where gibberellin was added in concentration upper then Avogrado’s number.


1995 ◽  
Vol 5 (3) ◽  
pp. 145-149 ◽  
Author(s):  
P. van der Toorn ◽  
B. D. McKersie

AbstractAt the time of protrusion, radicles of lettuce seeds became susceptible to desiccation damage. Concomitant with radicle elongation, both the reducing sugar content and the amount of lipid-aldehydes increased. The role of the hydroxy- and lipid-aldehydes in browning reactions that occurred during desiccation and re-imbibition was analysed. It was concluded that both types of aldehydes reacted with amino-groups during desiccation and that the increase in lipid-aldehydes during germination could be due to the augmented reducing sugar content. Therefore, the high reducing sugar content in radicles of germinated lettuce seeds may be the primary source of hydroxyl radicals, and thereby, a principal cause of desiccation damage.


2020 ◽  
Vol 8 (4) ◽  
pp. 540
Author(s):  
Glenda Sallaku ◽  
Gjok Vuksani ◽  
Astrit Balliu

The effects of different environment temperatures, priming treatments and priming durations on germination parameters and early growth of pepper seedlings were investigated. Seeds were hydro primed in distilled water, or osmo primed in two different water potentials (-0.15 and -0.5 MPa) of polyethylene glycol (PEG) 8000. Both, hydro primed and osmo primed seeds were subject of two different imbibitions periods, 48 and 72 hrs. In addition to primed seeds, an equal sample of non primed seeds was included in the experiment as a control variant. Following priming, the seeds were subjected to germination tests at two different environment temperatures, respectively 18 0C and 24 0C. In general, there was no significant effect of hydro priming on germination parameters or early growth of pepper seedlings. On the contrary, significantly higher germination percentage and germination speed and significantly shorter mean germination time than the non primed seeds were obtained due to seed osmo priming by PEG. Similarly, an enhanced seedling growth, especially under suboptimum temperature conditions was evidenced in PEG primed seedlings. Apart from germination speed, the increase of PEG concentration from -0.15 to -0.5 MPa had no effect to germination parameters of pepper seeds. The extent of the imbibitions period over 48 hrs did also have no benefits on germination parameters.


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