scholarly journals Organic exudates promote Fe(II) oxidation in Fe limited cultures of <i>Trichodesmium erythraeum</i>

2017 ◽  
Author(s):  
Hanieh T. Farid ◽  
Kai G. Schulz ◽  
Andrew L. Rose
Keyword(s):  
Rate Constants ◽  
Oxidation Rate ◽  
Growth Cycle ◽  
Batch Cultures ◽  
Trichodesmium Erythraeum ◽  
Luminol Chemiluminescence ◽  
Significant Difference ◽  
Kinetics Of ◽  
Best Fit ◽  
Extracellular Environment

Abstract. A luminol chemiluminescence method was employed to study the oxidation kinetics of Fe(II) in both the absence and the presence of organic exudates released by the marine cyanobacterium Trichodesmium erythraeum. The apparent Fe(II) oxidation rate constant was studied for batch cultures grown with varying Fe' concentrations of 0.05–0.29 and 1.44–2.03 nmol L−1 at pH ranges from 8.1–8.6, corresponding to the change in pH in the cultures during the entire growth cycle. Fe(II) oxidation was accelerated when cells were growing exponentially and gradually decreased towards the stationary phase, consistent with the presence of organic exudates. The best fit of the kinetic model to the data also demonstrated clear differences in apparent Fe(II) oxidation rate constants during different growth phases. However, no significant difference was observed in oxidation rate constants between the two Fe' treatments. These findings suggest that Trichodesmium releases organic compounds into the extracellular environment that influence Fe redox chemistry, potentially affecting Fe bioavailability, and that the nature of the Fe(II) complexes formed is not influenced by Fe limitation of the organism's growth.

Conversion between permeability states of IP3 receptors in cultured smooth muscle cells

1995 ◽  
Vol 269 (3) ◽  
pp. C813-C818 ◽  
Author(s):  
T. Sugiyama ◽  
W. F. Goldman
Keyword(s):  
Rate Constants ◽  
Time Course ◽  
Ip3 Receptors ◽  
A7r5 Cells ◽  
High Conductance State ◽  
Cultured Smooth Muscle Cells ◽  
Induced Changes ◽  
Kinetics Of ◽  
Best Fit ◽  
Conductance State

The kinetics of the effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ in the sarcoplasmic reticulum (SR) were studied in saponin-permeabilized A7r5 cells. At 0.1 microM, IP3 elicited slow monoexponential declines in SR free Ca2+ concentration ([Ca2+]SR). For IP3 concentration ([IP3]) = 0.2-100 microM, evoked declines in [Ca2+]SR were biphasic and best fit as the sum of two first-order processes with rate constants kfast and kslow. The kfast varied as a function of [IP3] over the range tested, whereas kslow was already maximal when [IP3] = 0.1 microM. To analyze SR Ca2+ release elicited by IP3, the rate constants for IP3-induced changes in the total SR Ca2+ content (kR) were calculated. kR was accurately described only when both [Ca2+]SR and [IP3] were considered together. kR was dependent on IP3 binding to receptors that existed in either of two states, a high-affinity low-conductance state (IP3RH) and a low-affinity high-conductance state (IP3RL). The permeability of IP3RL was 12.28 times larger than that of IP3RH, and the conversion between permeability states as well as changes in both the affinity and cooperativity with which IP3 was bound to IP3RL were mediated by SR Ca2+. This SR Ca(2+)-dependent modulation of the characteristics of IP3 receptors forms the basis for the biphasic time course characteristic of IP3-evoked SR Ca2+ release.

Hydrogenation of alkenes on a nickel-tungsten-alumina catalyst

1981 ◽  
Vol 46 (9) ◽  
pp. 2076-2082 ◽  
Author(s):  
Jan Uchytil ◽  
Eva Kočová ◽  
Miloš Kraus
Keyword(s):  
Molecular Weight ◽  
Rate Constants ◽  
The Other ◽  
Alumina Catalyst ◽  
Boiling Points ◽  
Kinetics Of ◽  
Best Fit ◽  
Nickel Tungsten

Eleven C2 to C8 alkenes were hydrogenated on a reduced sulphided industrial nickel-tungsten catalyst. Kinetics of the reaction was investigated in detail for ethylene and data were correlated by different equations. Best fit was obtained by three equations of the Langmuir-Hinshelwood type and these were used to describe hydrogenation of the other alkenes. Logarithms of the rate constants obtained were correlated linearly with the sum of steric constants E0s of substituents of semihydrogenated state R1R2R3C-*. Also adsorption coefficients of alkenes were structure dependent, increased with molecular weight of alkene and correlated roughly with boiling points.

Functional Fractionation of Platelets: Aggregation Kinetics and Glycoprotein Labeling of Differing Platelet Populations

1982 ◽  
Vol 48 (02) ◽  
pp. 211-216 ◽  
Author(s):  
V M Haver ◽  
A R L Gear
Keyword(s):  
Galactose Oxidase ◽  
Aggregation Kinetics ◽  
Maximal Rate ◽  
Membrane Glycoproteins ◽  
Whole Cells ◽  
Reversible Aggregation ◽  
Significant Difference ◽  
Small Doses ◽  
Major Loss ◽  
Kinetics Of

SummaryPlatelet heterogeneity has been studied with a technique called functional fractionation which employs gentle centrifugation to yield subpopulations (“reactive” and “less-reactive” platelets) after exposure to small doses of aggregating agent. Aggregation kinetics of the different platelet populations were investigated by quenched-flow aggregometry. The large, “reactive” platelets were more sensitive to ADP (Ka = 1.74 μM) than the smaller “less-reactive” platelets (Ka = 4.08 μM). However, their maximal rate of aggregation (Vmax, % of platelets aggregating per sec) of 23.3 was significantly lower than the “less-reactive” platelets (Vmax = 34.7). The “reactive” platelets had a 2.2 fold higher level of cyclic AMP.Platelet glycoproteins were labeled using the neuraminidase-galactose oxidase – [H3]-NaBH4 technique. When platelets were labeled after reversible aggregation, the “reactive” platelets showed a two-fold decrease in labeling efficiency (versus control platelets). However, examination of whole cells or membrane preparations from reversibly aggregated platelets revealed no significant difference in Coomassie or PAS (Schiff) staining.These results suggest that the large, “reactive” platelets are more sensitive to ADP but are not hyperaggregable in a kinetic sense. Reversible aggregation may cause a re-orientation of membrane glycoproteins that is apparently not characterized by a major loss of glycoprotein material.

Submerged upflow biotower operation and computer simulation

1994 ◽  
Vol 30 (11) ◽  
pp. 143-146
Author(s):  
Ronald D. Neufeld ◽  
Christopher A. Badali ◽  
Dennis Powers ◽  
Christopher Carson
Keyword(s):  
Computer Simulation ◽  
Benzoic Acid ◽  
Computer Model ◽  
Rate Constants ◽  
Batch Mode ◽  
Operational Conditions ◽  
First Order ◽  
Low Concentrations ◽  
Biological Transformation ◽  
Kinetics Of

A two step operation is proposed for the biodegradation of low concentrations (&lt; 10 mg/L) of BETX substances in an up flow submerged biotower configuration. Step 1 involves growth of a lush biofilm using benzoic acid in a batch mode. Step 2 involves a longer term biological transformation of BETX. Kinetics of biotransformations are modeled using first order assumptions, with rate constants being a function of benzoic acid dosages used in Step 1. A calibrated computer model is developed and presented to predict the degree of transformation and biomass level throughout the tower under a variety of inlet and design operational conditions.

Oxidation of (1-hydroxybenzyl)ferrocene and its derivatives substituted in phenyl ring with bis(triphenylsilyl) chromate

1982 ◽  
Vol 47 (12) ◽  
pp. 3375-3380 ◽  
Author(s):  
Jaroslav Holeček ◽  
Karel Handlíř ◽  
Milan Nádvorník ◽  
Milan Vlček
Keyword(s):  
Linear Dependence ◽  
Rate Constants ◽  
Oxidation Rate ◽  
Phenyl Ring ◽  
Equilibrium Constants ◽  
Aprotic Solvents ◽  
Protonation Equilibrium ◽  
Oxidation Of Alcohols ◽  
Hammett Σ Constants ◽  
Sulphuric Acid Medium

Kinetics have been studied of oxidation of (1-hydroxybenzyl)ferrocenes substituted in phenyl ring with bis(triphenylsilyl) chromate in benzene solutions as well as protonation of these alcohols in sulphuric acid medium. Logarithms of the oxidation rate constants (kobs, 20-40 °C) and those of the protonation equilibrium constants (KR+, 25 °C) show linear dependence on the Hammett σ constants, the ρ constant values being -0.86 to -0.40 and -2.50, respectively. These negative values suggest that the both processes are influenced by the same effects and confirm the mechanism proposed earlier for oxidation of alcohols with ferrocenyl substituent by action of bis(triphenylsilyl) chromate in aprotic solvents.

Kinetics of hydrolysis of peroxodisulphate ions in acidic medium to peroxomonosulphuric acid

1981 ◽  
Vol 46 (5) ◽  
pp. 1229-1236 ◽  
Author(s):  
Jan Balej ◽  
Milada Thumová
Keyword(s):  
Ionic Strength ◽  
Rate Constants ◽  
Acidic Medium ◽  
Measured Data ◽  
Molar Ratios ◽  
Starting Solution ◽  
Kinetics Of Hydrolysis ◽  
Rate Of Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of

The rate of hydrolysis of S2O82- ions in acidic medium to peroxomonosulphuric acid was measured at 20 and 30 °C. The composition of the starting solution corresponded to the anolyte flowing out from an electrolyser for production of this acid or its ammonium salt at various degrees of conversion and starting molar ratios of sulphuric acid to ammonium sulphate. The measured data served to calculate the rate constants at both temperatures on the basis of the earlier proposed mechanism of the hydrolysis, and their dependence on the ionic strength was studied.

Kinetics of the reaction between hexamethylenediisocyanate and 1-pentanol

1983 ◽  
Vol 48 (11) ◽  
pp. 3279-3286
Author(s):  
Slavko Hudeček ◽  
Miloslav Bohdanecký ◽  
Ivana Hudečková ◽  
Pavel Špaček ◽  
Pavel Čefelín
Keyword(s):  
Liquid Chromatography ◽  
Rate Constants ◽  
Reversed Phase ◽  
Order Reaction ◽  
Second Order ◽  
Second Order Reaction ◽  
Reversed Phase Liquid Chromatography ◽  
Consecutive Reactions ◽  
Phase Liquid ◽  
Kinetics Of

The reaction between hexamethylenediisocyanate and 1-pentanol in toluene was studied by means of reversed-phase liquid chromatography. By employing this method, it was possible to determine all components of the reaction mixture including both products, i.e. N-(6-isocyanate hexyl)pentylcarbamate and N,N'-bis(pentyloxycarbonyl)hexamethylenediamine. Relations for the calculation of kinetic constants were derived assuming a competitive consecutive second-order reaction. It was demonstrated that the reaction involved in this case is indeed a second-order reaction, and the rate constants of the first and second consecutive reactions were determined.

Kinetics of esterification of monoisopropylphenols with phosphoryl trichloride

1989 ◽  
Vol 54 (5) ◽  
pp. 1311-1317
Author(s):  
Miroslav Magura ◽  
Ján Vojtko ◽  
Ján Ilavský
Keyword(s):  
Liquid Phase ◽  
Rate Constants ◽  
Reaction Rates ◽  
Activation Energies ◽  
The Individual ◽  
Kinetics Of

The kinetics of liquid-phase isothermal esterification of POCl3 with 2-isopropylphenol and 4-isopropylphenol have been studied within the temperature intervals of 110 to 130 and 90 to 110 °C, respectively. The rate constants and activation energies of the individual steps of this three-step reaction have been calculated from the values measured. The reaction rates of the two isomers markedly differ: at 110 °C 4-isopropylphenol reacts faster by the factors of about 7 and 20 for k1 and k3, respectively. This finding can be utilized in preparation of mixed triaryl phosphates, since the alkylation mixture after reaction of phenol with propene contains an excess of 2-isopropylphenol over 4-isopropylphenol.

Determination of the Nucleophilicity of Tricarbonyliron Coordinated Cyclohepta-1,3,5-triene

1999 ◽  
Vol 64 (11) ◽  
pp. 1770-1779 ◽  
Author(s):  
Herbert Mayr ◽  
Karl-Heinz Müller
Keyword(s):  
Gibbs Energy ◽  
Ambient Temperature ◽  
Rate Constants ◽  
Second Order ◽  
Order Rate ◽  
Electrophilic Additions ◽  
Kinetics Of

The kinetics of the electrophilic additions of four diarylcarbenium ions (4a-4d) to tricarbonyl(η4-cyclohepta-1,3,5-triene)iron (1) have been studied photometrically. The second-order rate constants match the linear Gibbs energy relationship log k20 °C = s(E + N) and yield the nucleophilicity parameter N(1) = 3.69. It is concluded that electrophiles with E ≥ -9 will react with complex 1 at ambient temperature.

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