Turbulence Scale in Compound Channel

2021 ◽  
Author(s):  
Jyotirmoy Barman ◽  
Jyotismita Taye ◽  
Bimlesh Kumar
Keyword(s):  
Morphological Changes ◽  
Experimental Investigations ◽  
Compound Channel ◽  
Velocity Data ◽  
Bank Angle ◽  
Turbulence Scale ◽  
Compound Channels ◽  
Laboratory Flume ◽  
High Flood

<p>The study of turbulence in a compound channel would address the nature of sedmient transport and bank erosion activity. The study would also give insights of embankment and levee breaches at the time of high flood. Experimental investigations were conducted on two compound channels of 31<sup>0</sup> and 45<sup>0</sup> bank angle in the laboratory flume to study the turbulece scale. Velocity data were recorded with Nortek Velocimeter at seven different locations (3 locations at the upstream, 3 locations at the downstream and 1 location at the middle) of the compound channel. Turbulence scale like Taylor microscale (λ<sub>T</sub>) estimates the length scale of the inertial sub- range. The Taylor scale is calculated as:</p><p><img src="https://contentmanager.copernicus.org/fileStorageProxy.php?f=gepj.0042175ca60061700501161/sdaolpUECMynit/12UGE&app=m&a=0&c=2968072e536f82f8e38df248a26aa4a7&ct=x&pn=gepj.elif&d=1" alt="" width="199" height="148"></p><p>The Taylor microscale analysis showed dominance in the main channel for 45<sup>0</sup> bank angle as compared to 31<sup>0</sup> bank angle. In the location of slope midpoint and floodplain region of the compound channel, Taylor microscale was more dominant for 31<sup>0</sup> bank angle. Another important observation in both the compound channels (31<sup>0</sup> and 45<sup>0</sup> bank angle) is the dominance of Taylor microscale at the upstream section of the channel as compared to the downstream part of the channel. The results from the study would help us to get a better understanding of the role of turbulence in the morphological changes in a compound channel with different bank angles.</p>

scholarly journals Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jayarami Reddy Medapati ◽  
Deepthi Rapaka ◽  
Veera Raghavulu Bitra ◽  
Santhosh Kumar Ranajit ◽  
Girija Sankar Guntuku ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Morphological Changes ◽  
Serum Levels ◽  
Cb1 Receptors ◽  
Protective Effects ◽  
Renal Hypertrophy ◽  
Necrosis Factor Alpha

Abstract Background The endocannabinoid CB1 receptor is known to have protective effects in kidney disease. The aim of the present study is to evaluate the potential agonistic and antagonistic actions and to determine the renoprotective potential of CB1 receptors in diabetic nephropathy. The present work investigates the possible role of CB1 receptors in the pathogenesis of diabetes-induced nephropathy. Streptozotocin (STZ) (55 mg/kg, i.p., once) is administered to uninephrectomised rats for induction of experimental diabetes mellitus. The CB1 agonist (oleamide) and CB1 antagonist (AM6545) treatment were initiated in diabetic rats after 1 week of STZ administration and were given for 24 weeks. Results The progress in diabetic nephropathy is estimated biochemically by measuring serum creatinine (1.28±0.03) (p < 0.005), blood urea nitrogen (67.6± 2.10) (p < 0.001), urinary microprotein (74.62± 3.47) (p < 0.005) and urinary albuminuria (28.31±1.17) (p < 0.0001). Renal inflammation was assessed by estimating serum levels of tumor necrosis factor alpha (75.69±1.51) (p < 0.001) and transforming growth factor beta (8.73±0.31) (p < 0.001). Renal morphological changes were assessed by estimating renal hypertrophy (7.38± 0.26) (p < 0.005) and renal collagen content (10.42± 0.48) (p < 0.001). Conclusions From the above findings, it can be said that diabetes-induced nephropathy may be associated with overexpression of CB1 receptors and blockade of CB1 receptors might be beneficial in ameliorating the diabetes-induced nephropathy. Graphical abstract

Tissue Ki67 proliferative index expression and pathological changes in hemodialysis arteriovenous fistulae: Preliminary single-center results

2021 ◽  
pp. 112972982110154
Author(s):  
Raffaella Mauro ◽  
Cristina Rocchi ◽  
Francesco Vasuri ◽  
Alessia Pini ◽  
Anna Laura Croci Chiocchini ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Hot Spot ◽  
Wall Thickening ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Group A ◽  
The Mean ◽  
Set Up ◽  
Group B

Background: Arteriovenous fistula (AVF) for hemodialysis integrates outward remodeling with vessel wall thickening in response to drastic hemodynamic changes. Aim of this study is to determine the role of Ki67, a well-established proliferative marker, related to AVF, and its relationship with time-dependent histological morphologic changes. Materials and methods: All patients were enrolled in 1 year and stratified in two groups: (A) pre-dialysis patients submitted to first AVF and (B) patients submitted to revision of AVF. Morphological changes: neo-angiogenesis (NAG), myointimal thickening (MIT), inflammatory infiltrate (IT), and aneurysmatic fistula degeneration (AD). The time of AVF creation was recorded. A biopsy of native vein in Group A and of arterialized vein in Group B was submitted to histological and immunohistochemical (IHC) analysis. IHC for Ki67 was automatically performed in all specimens. Ki67 immunoreactivity was assessed as the mean number of positive cells on several high-power fields, counted in the hot spots. Results: A total of 138 patients were enrolled, 69 (50.0%) Group A and 69 (50.0%) Group B. No NAG or MIT were found in Group A. Seven (10.1%) Group A veins showed a mild MIT. Analyzing the Group B, a moderate-to-severe MIT was present in 35 (50.7%), IT in 19 (27.5%), NAG in 37 (53.6%); AD was present in 10 (14.5%). All AVF of Group B with the exception of one (1.4%) showed a positivity for Ki67, with a mean of 12.31 ± 13.79 positive cells/hot spot (range 0–65). Ki67-immunoreactive cells had a subendothelial localization in 23 (33.3%) cases, a myointimal localization in SMC in 35 (50.7%) cases. The number of positive cells was significantly correlated with subendothelial localization of Ki67 ( p = 0.001) and with NA ( p = 0.001). Conclusions: Native veins do not contain cycling cells. In contrast, vascular cell proliferation starts immediately after AVF creation and persists independently of the time the fistula is set up. The amount of proliferating cells is significantly associated with MIT and subendothelial localization of Ki67-immunoreactive cells, thus suggesting a role of Ki-67 index in predicting AVF failure.

scholarly journals The Neuroinflammatory Role of Pericytes in Epilepsy

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 759
Author(s):  
Gaku Yamanaka ◽  
Fuyuko Takata ◽  
Yasufumi Kataoka ◽  
Kanako Kanou ◽  
Shinichiro Morichi ◽  
...  
Keyword(s):  
Proinflammatory Cytokines ◽  
Current Knowledge ◽  
Morphological Changes ◽  
Cell Damage ◽  
Neurovascular Unit ◽  
Experimental Studies ◽  
Intractable Epilepsy ◽  
In Vivo Studies

Pericytes are a component of the blood–brain barrier (BBB) neurovascular unit, in which they play a crucial role in BBB integrity and are also implicated in neuroinflammation. The association between pericytes, BBB dysfunction, and the pathophysiology of epilepsy has been investigated, and links between epilepsy and pericytes have been identified. Here, we review current knowledge about the role of pericytes in epilepsy. Clinical evidence has shown an accumulation of pericytes with altered morphology in the cerebral vascular territories of patients with intractable epilepsy. In vitro, proinflammatory cytokines, including IL-1β, TNFα, and IL-6, cause morphological changes in human-derived pericytes, where IL-6 leads to cell damage. Experimental studies using epileptic animal models have shown that cerebrovascular pericytes undergo redistribution and remodeling, potentially contributing to BBB permeability. These series of pericyte-related modifications are promoted by proinflammatory cytokines, of which the most pronounced alterations are caused by IL-1β, a cytokine involved in the pathogenesis of epilepsy. Furthermore, the pericyte-glial scarring process in leaky capillaries was detected in the hippocampus during seizure progression. In addition, pericytes respond more sensitively to proinflammatory cytokines than microglia and can also activate microglia. Thus, pericytes may function as sensors of the inflammatory response. Finally, both in vitro and in vivo studies have highlighted the potential of pericytes as a therapeutic target for seizure disorders.

scholarly journals A Novel Model of Cancer-Induced Peripheral Neuropathy and the Role of TRPA1 in Pain Transduction

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Ahmad Maqboul ◽  
Bakheet Elsadek
Keyword(s):  
Immune Cells ◽  
Peripheral Nerves ◽  
Morphological Changes ◽  
Nerve Fibres ◽  
Cold Allodynia ◽  
Sciatic Nerves ◽  
Pain Transduction ◽  
Thermal Stimuli ◽  
Novel Model

Background. Models of cancer-induced neuropathy are designed by injecting cancer cells near the peripheral nerves. The interference of tissue-resident immune cells does not allow a direct contact with nerve fibres which affects the tumor microenvironment and the invasion process. Methods. Anaplastic tumor-1 (AT-1) cells were inoculated within the sciatic nerves (SNs) of male Copenhagen rats. Lumbar dorsal root ganglia (DRGs) and the SNs were collected on days 3, 7, 14, and 21. SN tissues were examined for morphological changes and DRG tissues for immunofluorescence, electrophoretic tendency, and mRNA quantification. Hypersensitivities to cold, mechanical, and thermal stimuli were determined. HC-030031, a selective TRPA1 antagonist, was used to treat cold allodynia. Results. Nociception thresholds were identified on day 6. Immunofluorescent micrographs showed overexpression of TRPA1 on days 7 and 14 and of CGRP on day 14 until day 21. Both TRPA1 and CGRP were coexpressed on the same cells. Immunoblots exhibited an increase in TRPA1 expression on day 14. TRPA1 mRNA underwent an increase on day 7 (normalized to 18S). Injection of HC-030031 transiently reversed the cold allodynia. Conclusion. A novel and a promising model of cancer-induced neuropathy was established, and the role of TRPA1 and CGRP in pain transduction was examined.

scholarly journals PRE- and POST-STORM LiDAR SURVEYS FOR ASSESSMENT OF IMPACT ON COASTAL EROSION

2019 ◽  
Vol XLII-3/W8 ◽  
pp. 261-266
Author(s):  
A.-L. Montreuil ◽  
M. Chen ◽  
A. Esquerré ◽  
R. Houthuys ◽  
R. Moelans ◽  
...  
Keyword(s):  
Management Practices ◽  
Morphological Changes ◽  
Storm Surges ◽  
High Water ◽  
Water Levels ◽  
Airborne Lidar ◽  
Coastline Change ◽  
The Mean ◽  
The Impact

<p><strong>Abstract.</strong> Sustainable management of the coastal resources requires a better understanding of the processes that drive coastline change. The coastline is a highly dynamic sea-terrestrial interface. It is affected by forcing factors such as water levels, waves, winds, and the highest and most severe changes occur during storm surges. Extreme storms are drivers responsible for rapid and sometimes dramatic changes of the coastline. The consequences of the impacts from these events entail a broad range of social, economic and natural resource considerations from threats to humans, infrastructure and habitats. This study investigates the impact of a severe storm on coastline response on a sandy multi-barred beach at the Belgian coast. Airborne LiDAR surveys acquired pre- and post-storm covering an area larger than 1 km<sup>2</sup> were analyzed and reproducible monitoring solutions adapted to assess beach morphological changes were applied. Results indicated that the coast retreated by a maximum of 14.7 m where the embryo dunes in front of the fixed dunes were vanished and the foredune undercut. Storm surge and wave attacks were probably the most energetic there. However, the response of the coastline proxies associated with the mean high water line (MHW) and dunetoe (DuneT) was spatially variable. Based on the extracted beach features, good correlations (r>0.73) were found between coastline, berm and inner intertidal bar morphology, while it was weak with the most seaward bars covered in the surveys. This highlights the role of the upper features on the beach to protect the coastline from storm erosion by reducing wave energy. The findings are of critical importance in improving our knowledge and forecasting of coastline response to storms, and also in its translation into management practices.</p>

scholarly journals Hepatic cryosurgery in experiment and clinic

2003 ◽  
Vol 2 (3) ◽  
pp. 56-61
Author(s):  
B. I. Alperovich ◽  
A. V. Potapov ◽  
V. N. Salo
Keyword(s):  
Low Temperature ◽  
Hepatic Resection ◽  
Morphological Changes ◽  
Hepatic Tissue ◽  
Experimental Investigations ◽  
Original Design ◽  
Hepatic Diseases ◽  
Electron And Light Microscopy ◽  
Design Characteristics ◽  
Sexually Mature

The aim of the investigation – to study changes in hepatic tissue resulting from the influence of low temperatures (– 195,8 °C) in experiments and clinic. Hepatic tissue investigations in experiment have been carried out using electron and light microscopy. During the surgery instruments of original design have been used – cryoscalpels and cryodestructors. Experiments have been carried out on 45 sexually mature dogs of both sexes. Animals have been separated into 3 groups. On the first group (consisting of 12 animals), in the acute experiment, there have been studied features of cryoscalpel design characteristics. In the second series of experiment (on 22 dogs) there have been made a comparative study of hepatic resection using cryoultrasonic scalpel and simple surgical scalpel by means of the study of hemorrhage quantity. In 11 cases there have been studied hepatic morphological changes affected by cryoeffect. By the effect of low temperature ice crystals are appeared in hepatic tissue intracellularly and outside the cell, they cause mechanical injury and rupture of hepatic tissue. In 45 days connective tissue elements are formed at the place of cryodestruction. The use of cryoultrasonic scalpel in hepatic resection in the experiment decreases the hemorrhage quantity by 1,26 times as compared to the traditional methods. Positive experimental investigations allowed to use the low temperature with success in clinical practice at 253 patients with different localized hepatic diseases with 13 lethal outcomes (5,1%). Thus, the used cryosurgical technique during surgical operations allows to decrease significantly the hemorrhage, to eliminate parenchymatous bleeding and to prevent relapses of disease both at growths and at parasitic hepatic affection.

scholarly journals Patient-individual cancer cell lines and tissue analysis delivers no evidence for a role of DNA virus infection on colorectal cancer oncogenesis

2019 ◽  
Author(s):  
Michael Gock ◽  
Marcel Kordt ◽  
Stephanie Matschos ◽  
Christina S. Mullins ◽  
Michael Linnebacher
Keyword(s):  
Cell Lines ◽  
Molecular Subtypes ◽  
Morphological Changes ◽  
Tissue Analysis ◽  
Oncogenic Viruses ◽  
Dna Viruses ◽  
Viral Particles ◽  
Specific Sequences

Abstract Background Several DNA viruses are highly suspicious to have oncogenic effects in humans. This study investigates the presence of potentially oncogenic viruses such as SV40, JCV, BKV and EBV in patient-derived colorectal carcinoma (CRC) cells typifying all molecular subtypes of CRC. Methods Sample material (gDNA and cDNA) of a total of 49 patient-individual CRC cell lines and corresponding primary material from 11 patients, including normal, tumor-derived and metastasis-derived tissue were analyzed for sequences of SV40, JVC, BKV and EBV using endpoint PCR. In addition, the susceptibility of CRC cells to JCV and BKV was examined using a long-term cultivation approach of patient-individual cells in the presence of viruses. Results No virus-specific sequences could be detected in all specimens. Likewise, no morphological changes were observed and no evidence for viral infection or integration could be provided after long term CRC cell cultivation in presence of viral particles. Conclusions In summary, the presented data suggest that there is no direct correlation between tumorigenesis and viral load and consequently no evidence for a functional role of the DNA viruses included into this analysis in CRC development.

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