scholarly journals AKTIVITAS LARVASIDA EKSTRAK ETANOL DAUN KEMUNING (Murraya paniculata JACK) TERHADAP LARVA Aedes aegypti

2020 ◽  
Vol 5 (2) ◽  
pp. 430
Author(s):  
Arifani Siswidiasari ◽  
Dewa Putu Agus Wahyu Erawan
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Preliminary Test ◽  
Negative Control ◽  
Probit Analysis ◽  
Yellow Leaf ◽  
Murraya Paniculata

Introduction: A research had been carried out to analyze the larvicidal activity of the ethanol extract of kemuning leaves (Murraya paniculata (L.) Jack.) against Aedes aegypti L. larvae. Method: The research began with extracting yellow leaves (Murraya paniculata (L.) Jack.) With a modified maceration method using 80% ethanol solvent until a thick extract is obtained, then a preliminary test is carried out to obtain LC5 and LC95 so that 5 concentrations of yellow leaf extract (Murraya paniculata (L.) Jack.) can be obtained which can be used as a real larvicide test. The actual larvicide test was carried out with 7 treatments, namely 1 negative control using Tween 80, 5 test treatments namely 1000 bpj yellow leaf extract, 3000 bpj, 5000 ppm, 7000 ppm and 9000 ppm and 1 positive control using Temephos 2 bpj. Observation of the test results was carried out after 24 hours by counting the number of larvae deaths in each treatment group. Result: The data obtained were analyzed by using probit analysis and obtained LC90 of yellow leaf extract (Murraya paniculata (L.) Jack.) which is 8330,291 bpj. The results showed that the yellow leaf extract (Murraya paniculata (L.) Jack.) was effective as a larvicidal activity with LC5 of 1000 bpj and LC95 of 9000 bpj.

scholarly journals Potensi Ekstrak Daun Marigold (Tagetes erecta L.) sebagai Larvasida terhadap Larva Aedes aegypti di Laboratorium

2018 ◽  
Vol 12 (2) ◽  
pp. 109-114
Author(s):  
Marini Marini ◽  
Vivin Mahdalena ◽  
Tanwirotun Ni'mah
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Larval Mortality ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Tagetes Erecta ◽  
Mosquito Vectors ◽  
Mosquito Repellent ◽  
Tagetes Erecta L

Abstract Control of mosquito vectors by using natural ingredients is being developed intensively. Marigold (Tagetes erecta) was widely used as a mosquito repellent plant. The results of research before had found that marigold leaf extract has a repulsive effect on Aedes aegypti mosquitoes. This study aimed to identify the potential of marigold leaf extract as larvacide against Ae. aegypti larvae in the laboratory. The material used in this research was extracts of marigold leaves. Larvacide test was carried out by dissolving of extract in water at the concentration of 2,000 ppm, 4,000 ppm, 6,000 ppm, 8,000 ppm, and 10,000 ppm. The larvae used was Ae. aegypti larvae as many as 25 larvae each five treatment of replications. Probit analysis was conducted on larval mortality after 24 hours of exposure. The results of the probit analysis showed that the estimated concentration of extracts that could result in larval mortality up to 95% (LC95) was 7,456 ppm. From the result we can conclude that ethanol extract of marigold leaves (T. erecta L. ) has the biolarvacidal activity against larvae. Abstrak Pengendalian vektor nyamuk dengan memanfaatkan bahan alam makin banyak dikembangkan. Marigold (T. erecta) merupakan tumbuhan yang banyak dimanfaatkan  sebagai tanaman pengusir nyamuk. Hasil penelitian sebelumnya menemukan bahwa ekstrak daun memiliki daya tolak terhadap nyamuk Ae. aegypti. Penelitian ini bertujuan untuk melihat potensi ekstrak daun marigold sebagai larvasida terhadap larva Ae. aegypti instar III di laboratorium. Bahan yang digunakan pada penelitian ini adalah ekstrak etanol daun marigold hasil ekstraksi dari penelitian sebelumnya. Uji larvasida dilakukan dengan melarutkan ekstrak dalam air pada konsentrasi 2.000 ppm, 4.000 ppm, 6.000 ppm, 8.000 ppm, dan 10.000 ppm. Larva yang digunakan yaitu larva Ae. aegypti sebanyak 25 larva tiap perlakuan dengan lima ulangan. Dilakukan analisis probit terhadap kematian larva setelah 24 jam paparan. Hasil analisa probit menunjukkan perkiraan konsentrasi ekstrak yang mampu mengakibatkan kematian larva hingga 95% (LC95 ) adalah 7.456 ppm. Ekstrak etanol daun marigold (T.erecta L.) memiliki potensi sebagai biolarvasida terhadap larva Ae. aegypti.  

scholarly journals Larvicidal Activity of Leaf Extract From Mauritiella armata (Aceraceae) on Aedes aegypti and Culex quinquefasciatus (Culicidae)

2022 ◽  
Vol 14 (2) ◽  
pp. 63
Author(s):  
Rosanna Lorrane Francisco dos Reis Matos ◽  
Núbia Nunes de Souza ◽  
Sandro Morais dos Santos ◽  
Aline Ferreira Rafael ◽  
Eduardo Robson Duarte ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Culex Quinquefasciatus ◽  
Leaf Extract ◽  
Mineral Water ◽  
Negative Control ◽  
Palm Tree ◽  
Negative Effects ◽  
Promising Alternative ◽  
Social Importance

The mosquitoes Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) are important vectors of several arboviruses, and are relevant public health problems. Conventional control, using chemical larvicides have selected resistant Culicidae populations and caused negative effects on the environment and human health. However, the use of plant extracts has represented a sustainable alternative for insect control. Popularly known as Xiriri, Mauritiella armata (Mart.) Burret (Aceraceae) is an abundant palm tree in Vereda ecosystems and has economic and social importance. In this study, the aim was to evaluate the larvicidal activity of the aqueous extract (AE) leaves of this plant on Ae. aegypti and Cx. quinquefasciatus larvae. The mortalities of larvae were analyzed after treatment with four concentrations of the extract, comparing with a negative control using mineral water. The AE promoted 100% efficacy against Ae. aegypti larvae at 7.9 mg/mL. The lethal concentration to promote 90% mortality of Cx. quinquefasciatus larvae was estimated at 30.57 mg/mL. After chromatographic analyses, flavonoids, catechin and carbohydrates were detected. AE from M. armata leaves presented high larvicidal activity against Ae. aegypti and Cx. quinquefasciatus, and represents a promising alternative to be used in vector control.

scholarly journals Antihyperglycemic activity of Centella asiatica (L.) Urb. leaf ethanol extract SNEDDS in zebrafish (Danio rerio)

2021 ◽  
Vol 19 (1) ◽  
pp. 184-188
Author(s):  
Farida Hayati ◽  
Lutfi Chabib ◽  
Faiza Dea Sekarraras ◽  
Wan Syarifah Faizah
Keyword(s):  
Glucose Solution ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Peg 400 ◽  
Good Preparation

Abstract This study aimed to identify the effectiveness of SNEDDS of Pegagan Leaf Ethanol Extract (PLE) to reduce fasting blood glucose (FBG) levels in zebrafish. Centella asiatica (L.) Urb. or pegagan is among the medicinal plants widely used to treat diabetes in Indonesia. Maceration was employed with 70% ethanol to obtain a viscous extract for the formulation of SNEDDS with Capryol 90, Tween 80, and PEG 400 (1:6:3). Antihyperglycemic testing was conducted on five groups, consisting of normal, positive control, negative control, P I treatment, and P II treatment. On Day 1, all except the normal group was induced with 300 mg alloxan and soaked in 2% glucose solution for 7 days. On day 8, the treatment consisted of 25 mg/2 L metformin for the positive control, 100 mg/2 L SNEDDS for P I, 200 mg/2 L SNEDDS for P II, and no treatment for the negative control. The SNEDDS characterization obtained 100.6 ± 3.12 nm particle size and −7.93 ± 0.66 mV zeta potential, indicating that the SNEDDS had fulfilled the requirements of good preparation. The antidiabetic activity test found a 69.90% decline in FBG levels in 100 mg/2 L SNEDDS and 72.20% in 200 mg/2 L SNEDDS.

scholarly journals LARVICIDAL ACTIVITY OF Caesalpinia ferrea MART. AND Lippia origanoides CHAM. AGAINST Aedes aegypti (LINNAEUS, 1762) (DIPTERA: CULICIDAE)

2020 ◽  
Vol 49 (1) ◽  
pp. 45-54
Author(s):  
Thiago Andre Santos de Andrade ◽  
Ivanise Maria de Santana ◽  
George Chaves Jimenez ◽  
Eulina Tereza Nery Farias ◽  
Lucia Oliveira de Macedo ◽  
...  
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Botanical Insecticide ◽  
Plant Metabolites ◽  
Important Public Health ◽  
Public Health Challenges ◽  
Ethanol Extracts

The control of Aedes aegypti has been considered one of the most important public health challenges worldwide. Chemical compounds have long been used for this purpose, but resistance to these molecules has also increased. Therefore, over the last few years several studies have focused on the development of alternative tools, particularly those based on plant metabolites. The purpose of this study was to assess the larvicidal activity of Caesalpinia ferrea and Lippia origanoides against Ae. aegypti. Larvae (L3) of Ae. aegypti Liverpool and Rockefeller strains, as well as of the Recife population were exposed to different concentrations  of C. ferrea (ranging from 13.1 to 105 mg/mL) and L. origanoides (ranging from 16.3 to 130 mg/mL), and the mortality rate was evaluated up to 48 hours after the beginning of the experiment. All tested groups and  ontrol group were quadruplicated. For C. ferrea, mortality ranged from 42.5% to 100% for Ae. aegypti Liverpool strain,from 67% to 100% for Ae. aegypti Rockfeller strain, and 57% to 100% for Ae. aegypti Recife population after 48 hours of larval exposure. For L. origanoides, the larvicidal activity ranged from 75% to 100% for Ae. aegypti Liverpool strain, from 61.5% to 100% for Ae. aegypti Rockfeller strain, and from 60.5% to 100% for Ae.aegypti Recife population. The hydro ethanol extract of C. ferrea and L. origanoides presented larvicidal activity against Ae. aegypti.KEY WORDS: Aedes aegypti; hydro ethanol extracts; Caesalpinia ferrea; Lippia sidoides; botanical insecticide; mosquitoes.

scholarly journals AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
I Nyoman Pugeg Aryantha ◽  
Wahyu Setyaji Dwiantara
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Beauveria Bassiana ◽  
Larvicidal Activity ◽  
Control Agent ◽  
Probit Analysis ◽  
Hexane Extract ◽  
Potato Dextrose Broth ◽  
Synthetic Pesticides ◽  
Biological Insect Control

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.   

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

2014 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Rahmawati Ekaputri ◽  
Sudarsono Sudarsono ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Fruit Extract ◽  
Vinca Rosea ◽  
Layer Chromatography

<p>Background: <em>Vinca rosea</em> is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of <em>Vinca rosea</em> was selected to investigation its larvicidal activity against the 3<sup>rd</sup> instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) <em>Aedes aegypti.</em>  Five concentrations of Vinca fruit extract were tested against the 3<sup>rd</sup> instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC<sub>50</sub> anf LC<sub>90</sub>) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC<sub>50</sub> and LC<sub>90</sub> values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.</p>

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

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