Phytochemical Assessment of Mimusops elengi Linn. Unripe fruits Methanol extracts using Modern Analytical Technique

2021 ◽  
pp. 3700-3704
Author(s):  
Bishwanath Mishra ◽  
Sujit Dash ◽  
Amaresh Chandra Sahoo ◽  
Prabhat Kumar Sahoo ◽  
Sazia Tabasum
Keyword(s):  
Methanol Extract ◽  
Pharmacological Action ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Molecular Formula ◽  
Analytical Technique ◽  
Modern Analytical Technique ◽  
Rf Values ◽  
Spectrometry Technique ◽  
Mimusops Elengi

Mimusops elengi Linn. is a plant with high medicinal and commercial value. Although several secondary metabolites have been reported from different species of this plant, there has been not much information available on the complete profile of phytochemical constituents in Mimusops elengi Linn. The HPTLC fingerprinting of methanol extract showed 6 peaks having maximum Rf values 0.10, 0.27, 0.34, 0.51, 0.72, and 0.80 at 254nm. The same extract showed 3 peaks having maximum Rf value 0.25, 0.29 and 0.49 at 366 nm. This study applies Gas chromatography-Mass spectrometry technique to determine the possible chemical components in the methanol extract of Mimusops elengi Linn. Unripened fruits reports for the first time most extensive profile of the plant. The determination and identification of bioactive chemical compounds is established and based on the peak area, retention time molecular weight, and molecular formula. GC-MS analysis of Mimusops elengi Linn. revealed the existence of the 2,2’ Methylenebis [3,4,6-trichloroanisole] which was found to be major component followed by Z-1,9-Hexadecadiene, Cyclopentaneundecanoic-acid, 4-Methyloctanoic acid and E-10-Dodecen-1-ol propionate. The results obtained may be helpful to the further study of pharmacological action for their promising utilization as therapeutic agents.

scholarly journals Antimicrobial Activity of Hand Lotion of Flower Mimusops elengi

2019 ◽  
Vol 7 (22) ◽  
pp. 3748-3756
Author(s):  
Bastian Arifin ◽  
Rosnani Nasution ◽  
Novi Desrianti ◽  
Marianne Marianne ◽  
Hira Helwati
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
Positive Control ◽  
Gas Chromatography Mass Spectrometry ◽  
Tropical Region ◽  
Organoleptic Properties ◽  
Flower Extract ◽  
Mimusops Elengi ◽  
Antibacterial And Antifungal

BACKGROUND: Aceh is a tropical region that is very many overgrown by various plants that have medicinal properties; one of them is M. elengi. M. elengi flower extract has the main content of triterpene and alcohol, that have antibacterial, antifungal, antioxidant and antineoplastic activity. Extraction of chemical compounds containing essential oils generally uses distillation, and get the small amounts of chemical compounds, while the maceration with n-hexane solvent, producing less active nonpolar compounds against S. aureus bacteria. AIM: Isolating the methanol extract from M. elengi flowers and test its antibacterial and antifungal activity. Furthermore, extracts with active concentrations are made into a lotion. METHODS: Methanol extract from M. elengi flower was characterised by gas chromatography-mass spectrometry, then tested for antibacterial and antifungal, then made into the lotion. The lotion was tested again for its antimicrobial activity, physical and organoleptic properties. RESULTS: The most abundant chemical compounds in an extract of M. elengi based on characterisation with GC-MS is Borneol L; (Bicyclo [2.2.1] heptane-2-ol, 1,7,7-trimethyl, as much as 82%). The methanol extract of M. elengi flower can inhibit the growth of S. aureus bacteria and C. albicans fungi, and also the lotion from methanol extract can inhibit the growth of S. aureus bacteria, but the M. elengi flower extract lotion cannot inhibit the growth of C. albicans fungi. The lotion inhibits the growth of S. aureus bacteria, from a concentration of methanol extract of 8% and 16%. Lotion with 16% methanol extract has 81.33% in activity power compared with positive control. The results of physical and organoleptic properties test, with the concentration of methanol extract of M. elengi 1, 2, 8, and 16%, have pH in the range of 6.6-8 (still in a safe range, 4.5-9 according to SNI 16-4399-1997). The lotion type is m/a, the spreading capacity of the lotion is 18.8 - 39.5 cm2. The power of adhesive at skin ranges from 1 minute 27 seconds to 3 minutes 7 seconds. The viscosity of the lotion ranges from 23,670-24,400 cP, this range is in the range based on SNI 16-4399-1996 (2000-50,000 cP), so the lotion is in a good category. The preferred lotion is at a concentration of 2%, in fragrance. CONCLUSION: Antibacterial activity of the lotion of methanol extract of M. elengi flower against S. aureus bacteria was the best at 16%, but could not inhibit the growth of C. albicans fungi. The most abundant compounds in methanol extract are Borneol L compounds; (Bicyclo [2.2.1] heptane-2-ol, 1,7,7-trimethyl. In general, the physical properties of this lotion meet the requirements of SNI16-4399-1996, SNI 16-4399-1997, and lotions which are preferred at a concentration of 2% in fragrance.

Uşak İli’nden Toplanan Taxus Baccata (L) Türünün Metanol Ekstresinin Yaprak, Dal ve Meyvesinde Biyolojik Aktivitenin Belirlenmesi

2019 ◽  
Vol 7 (10) ◽  
pp. 1533
Author(s):  
Yasemin Karafakıoglu
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Maximum Amount ◽  
Chemical Components ◽  
Taxus Baccata ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Future Studies ◽  
Different Parts

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

scholarly journals Characterization of the Volatile Profile of Cultivated and Wild-Type Italian Celery (Apium graveolens L.) Varieties by HS-SPME/GC-MS

2021 ◽  
Vol 11 (13) ◽  
pp. 5855
Author(s):  
Samantha Reale ◽  
Valter Di Cecco ◽  
Francesca Di Donato ◽  
Luciano Di Martino ◽  
Aurelio Manzi ◽  
...  
Keyword(s):  
Solid Phase ◽  
Principal Component ◽  
Apium Graveolens ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Chemical Components ◽  
Volatile Profile ◽  
Wild Type ◽  
Multivariate Statistical ◽  
Clear Differentiation

Celery (Apium graveolens L.) is a vegetable belonging to the Apiaceae family that is widely used for its distinct flavor and contains a variety of bioactive metabolites with healthy properties. Some celery ecotypes cultivated in specific territories of Italy have recently attracted the attention of consumers and scientists because of their peculiar sensorial and nutritional properties. In this work, the volatile profiles of white celery “Sedano Bianco di Sperlonga” Protected Geographical Indication (PGI) ecotype, black celery “Sedano Nero di Torricella Peligna” and wild-type celery were investigated using head-space solid-phase microextraction combined with gas-chromatography/mass spectrometry (HS-SPME/GC-MS) and compared to that of the common ribbed celery. Exploratory multivariate statistical analyses were conducted using principal component analysis (PCA) on HS-SPME/GC-MS patterns, separately collected from celery leaves and petioles, to assess similarity/dissimilarity in the flavor composition of the investigated varieties. PCA revealed a clear differentiation of wild-type celery from the cultivated varieties. Among the cultivated varieties, black celery “Sedano Nero di Torricella Peligna” exhibited a significantly different composition in volatile profile in both leaves and petioles compared to the white celery and the prevalent commercial variety. The chemical components of aroma, potentially useful for the classification of celery according to the variety/origin, were identified.

scholarly journals Analysis of electrocautery generated smoke by chromatographic-mass spectrometry

2016 ◽  
Vol 43 (2) ◽  
pp. 124-128 ◽  
Author(s):  
JEFFERSON KALIL ◽  
FRANCISCO B. T. PESSINE ◽  
CARLOS H. V. FIDELIS ◽  
FABIO H. MENEZES ◽  
PAULO CESAR RODRIGUES PALMA
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Qualitative And Quantitative Analysis ◽  
Porcine Tissue ◽  
Qualitative And Quantitative ◽  
Hyphenated Technique ◽  
Muscular Tissues ◽  
Chromatographic Mass ◽  
Liver Tissues

ABSTRACT Objective: to analyze the chemical components of the smoke from electrocautery from coagulating muscle and liver tissues of pigs. Methods: we collected smoke produced by electrocautery applied to porcine tissue in previously evacuated bottles, with qualitative and quantitative analysis of the compounds present through the hyphenated technique gas chromatography / mass spectrometry. Results: there was a majority of decanal aldehyde in the fumes from the subcutaneous, muscle and liver tissues. Fumes of subcutaneous and muscular tissues also showed the presence of hexanal and phenol. In the fumes of subcutaneous and liver tissues we also found toluene and limonene and, finally, nonanal smoke was present in the muscle and liver tissues. Conclusion: there is increasing evidence showing that smoke from electrocautery used in subcutaneous, muscle and liver tissue is harmful to human health. Thus, there is need to reduce exposure to it or wear masks with filters capable of retaining these particles.

scholarly journals Enhanced Antibacterial Efficiency of Cellulosic Fibers: Microencapsulation and Green Grafting Strategies

Coatings ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 980 ◽  
Author(s):  
Dorra Dridi ◽  
Aicha Bouaziz ◽  
Sondes Gargoubi ◽  
Abir Zouari ◽  
Fatma B’chir ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Cotton Fabrics ◽  
Gas Chromatography Mass Spectrometry ◽  
Wall Material ◽  
Chemical Components ◽  
Syzygium Aromaticum ◽  
Disk Diffusion Test ◽  
Cellulosic Fibers

We report an analysis of chemical components of essential oils from barks of Ceylon cinnamon and cloves of Syzygium aromaticum and an investigation of their antibacterial activity. The components of oils were determined by using Gas Chromatography/Mass Spectrometry (GC-MS) analysis, and the antimicrobial activity was assessed by the disk diffusion test. The synergic effect of essential oils mixture (cinnamon oil and clove oil) was evaluated. Antimicrobial properties were conferred to cellulosic fibers through microencapsulation using citric acid as a green binding agent. Essential oil mixture was encapsulated by coacervation using chitosan as a wall material and sodium hydroxide as a hardening agent. The diameter of the produced microcapsules varies between 12 and 48 μm. Attachment of the produced microcapsules onto cotton fabrics surface was confirmed by Attenuated Total Reflectance-Fourier Transformed Infrared (ATR-FTIR) spectroscopy, optical microscopy and Scanning Electron Microscopy (SEM) analysis. The results show that microcapsules were successfully attached on cotton fabric surfaces, imparting antibacterial activity without significantly affecting their properties. The finished cotton fabrics exhibited good mechanical properties and wettability.

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