Chemical Compounds and Antimicrobial Activities of Actinomycetes Isolates from BRIS Soil

2021 ◽  
pp. 4783-4788
Author(s):  
Ali Arkan Majhool ◽  
Hamidah Idris ◽  
Wan Mohd Nuzul Hakimi ◽  
Muhd Danish Daniel Abdullah
Keyword(s):  
Micrococcus Luteus ◽  
Chemical Compounds ◽  
Antimicrobial Activities ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Gram Negative Bacteria ◽  
16S Rrna Gene Sequences ◽  
Gram Positive Bacteria ◽  
Wide Range ◽  
Drug Leads

The existing study examined three actinomycetes isolated from Beach Ridges Interspersed with Swales (BRIS) soil where the morphological properties were examined and chemical compounds of their metabolite extracts were ana; ysed. Based on phylogenetic analysis of 16S rRNA gene sequences, the strains were identified as Streptomyces sp. AA13, Amycolatopsis sp. AA12 and Micromonosporasp. AA141. Antimicrobial activities of the extracts were tested against a panel of microorganisms that consist of Escherichia coli DSM 30083, Bacillus subtilis DSM 10, Pseudomonas fluorescens DSM 50090, Klebsiella pneumoniae DSM 30104, Micrococcus luteus DSM 20030, and Saccharomyces cerevisiae. Results revealed a wide range of antimicrobial activities produced by the isolates at different concentration of each extracts. The extracts were found to show stronger antimicrobial activities against gram-negative bacteria compared to gram-positive bacteria, while no activities were shown when tested against fungi. Thirty-nine compounds were detected both from Streptomycessp. AA13 and Amycolatopsissp. AA12, while thirty-eight compounds from Micromonosporasp. AA141 using GC-MS. This Study Demonstrate the ability of actinomycetes in producing variety type of compounds with antimicrobial activities that may be the potential candidates as drug leads.

scholarly journals SYNTHESIS OF ARYL (5-SUBSTITUTED BENZOFURAN-2-YL) CARBAMATE DERIVATIVES AS ANTIMICROBIAL AGENTS

2017 ◽  
Vol 10 (3) ◽  
pp. 377
Author(s):  
Shankar N. Budhwani ◽  
Shailendra Sharma ◽  
Navanath Kalyane
Keyword(s):  
1H Nmr ◽  
Spectroscopic Data ◽  
Mass Spectra ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Substituted Phenols ◽  
Pharmacological Activities ◽  
Gram Positive Bacteria ◽  
Wide Range

ABSTRACTObjectives: Benzofurans are very interesting heterocycles, which are available in nature and show a wide range of pharmacological activities, viz.,antifungal, antibacterial, antitumor, antimalarial and antioxidant activity.Methods: A convenient method for the preparation of aryl (5-substituted benzofuran-2-yl) carbamate derivatives 6a-6j has been developed. Thetarget compounds 1-(5-nitrobenzofuran-2-yl)-3-arylurea (6a-6e) and 1-(5-bromobenzofuran-2-yl)-3arylurea (6f-6j) have been prepared by reacting5-nitrobenzofuran-2-carbonyl azide 5a or 5-bromobenzofuran-2-carbonyl azide 5b with substituted phenols in reasonable overall yields. Allthe synthesized compounds were characterized using Fourier transform infrared (FTIR), 1H NMR and mass spectrometry and were subjected toantimicrobial screening against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), two Gram-negative bacteria (Escherichiacoli and Pseudomonas aeruginosa), and two fungi (Candida albicans and Aspergillus niger) using two-fold dilution method.Results and Discussion: All the values of FTIR, 1H NMR and mass spectra were found to be prominent. The results indicate that synthesized compound6i showed potent antimicrobial activity comparable to standard.Conclusion: The detailed synthesis, spectroscopic data, and antimicrobial activities of synthesized compounds were reported.Keywords: Benzofuran, Antibacterial activity, Antifungal activity.

scholarly journals Antimicrobial Activities of Mefloquine and a Series of Related Compounds

2000 ◽  
Vol 44 (4) ◽  
pp. 848-852 ◽  
Author(s):  
C. M. Kunin ◽  
W. Y. Ellis
Keyword(s):  
Staphylococcus Epidermidis ◽  
Antimicrobial Activities ◽  
Walter Reed Army Institute ◽  
Gram Negative Bacteria ◽  
Antimicrobial Drugs ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Resistant Strains ◽  
Vancomycin Resistant ◽  
Related Compounds

ABSTRACT Mefloquine was found to have bactericidal activity against methicillin- and fluoroquinolone-susceptible and -resistant strains ofStaphylococcus aureus and Staphylococcus epidermidis and gentamicin- and vancomycin-resistant strains ofEnterococcus faecalis and Enterococcus faecium. The MICs were 16 μg/ml, and the minimal bactericidal concentrations (MBCs) were 16 to 32 μg/ml. These concentrations cannot be achieved in serum. Mefloquine was active at a more achievable concentration against penicillin-susceptible and -resistant Streptococcus pneumoniae, with MICs of 0.2 to 1.5 μg/ml. Mefloquine was not active against gram-negative bacteria and yeasts. In an attempt to find more active derivatives, 400 mefloquine-related compounds were selected from the chemical inventory of The Walter Reed Army Institute of Research. We identified a series of compounds containing a piperidine methanol group attached to pyridine, quinoline, and benzylquinoline ring systems. These had activities similar to that of mefloquine against S. pneumoniae but were far more active against other gram-positive bacteria (MICs for staphylococci, 0.8 to 6.3 μg/ml). They had activities similar to that of amphotericin B againstCandida spp. and Cryptococcus neoformans. Combinations of the compounds with gentamicin and vancomycin were additive against staphylococci and pneumococci. The MIC and MBC of gentamicin were decreased by four- to eightfold when this drug was combined with limiting dilutions of the compounds. There was no antagonism with other antimicrobial drugs. The compounds were rapidly bactericidal. They appear to act by disrupting cell membranes. Combinations of the compounds with aminoglycoside antibiotics may have potential for therapeutic use.

scholarly journals Chemical Composition, Antimicrobial, Cytotoxic and Antiplasmodial Activities of Three Sponges from Buton Islands, Indonesia

2017 ◽  
Vol 22 (3) ◽  
pp. 147
Author(s):  
Masteria Yunovilsa Putra ◽  
Tri Aryono Hadi
Keyword(s):  
Fungal Pathogen ◽  
Brine Shrimp ◽  
Antimicrobial Activities ◽  
Marine Sponges ◽  
Sponge Species ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Gram Positive Bacteria ◽  
Ms Analysis ◽  
Inhibition Zones

GC-MS analysis of the crude extracts of three different species of Indonesian marine sponges has been carried out for identification of bioactive compounds. The GC-MS analysis from Haliclona (Gellius) sp., Lamellodysidea herbacea, and Spheciospongia inconstans revealed the presence of 23, 21, 19 various compounds, respectively and mainly sterols and fatty acids. All the sponge species has been evaluated for antimicrobial activities, cytotoxicity using brine shrimp lethality bioassay and heme polymerization inhibitory activity assay for antiplasmodial activity. In this study, all the sponge species showed antimicrobial activities against at leastone of the test strains. Among them, the extract of sponge Lamellodysidea herbacea displayed activity against two Gram-positive bacteria (S. aureus and B. subtilis) and the Gram-negative bacteria V. cholerae, with inhibition zones of 10.3, 9.2 and 9.5 mm, respectively. The sponge Haliclona (Gellius) sp., showed significant activity against fungal pathogen C. albicans. The sponge Haliclona (Gellius) sp., displayed the ability to inhibit heme polymerization indicating an anti-Plasmodium function and also showed potent cytotoxic activity against the brine shrimp Artemia sp. Keywords: GC-MS analysis, antimicrobial, sponges,bioactive

scholarly journals SKRENING BAKTERI TOLERAN PESTISIDA DENGAN BAHAN AKTIF KLORANTRANILIPROL ASAL TANAH PERTANIAN BATURITI TABANAN BALI

2017 ◽  
Vol 21 (1) ◽  
pp. 1
Author(s):  
NI KADEK WIWIK SINTA DEWI ◽  
IDA BAGUS GEDE DARMAYASA ◽  
I KETUT SUNDRA
Keyword(s):  
Large Scale ◽  
Chemical Compounds ◽  
Mineral Medium ◽  
Gram Negative Bacteria ◽  
Soil Mineral ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Numerous Problem ◽  
Stage 1

In Indonesia agriculture practice often used the large scale pesticide application such as insecticide, herbicide, and fungicide. The wide use of toxic pesticide has created numerous problem in increasing environtmental hazard to human and to other animals. Many of soil bacteria had important role to degrading chemical compounds into simpler compounds as a bioremediation agent. The aim of this study was to screen the Chlorantraniliprole tolerant bacteria using soil mineral medium with Prevathon pesticide addition, also teo identificate the species of bacteria. This research was conducted at Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Udayana University. The research was done in three analysis, (1) bacteria test on Prevathon pesticide addition to mineral medium treatment, (2) characterization of bacteria, (3) Identification of pesticide tolerant bacteria with BD BBL Crystal Enteric/Non FermenterID Kit. The results showed that mineral medium with the addition of pesticides Prevathon treatment able to provide a significant different effect on the enrichment stage 1, stage 2 and stage 3 (P<0.05), there was 5 isolates pesticide tolerant bacteria that isolated from Baturiti Tabanan cultivated soil that was BSP 1, BSP 2, BSP 3 known as gram negative bacteria, and BSP 4, BSP 5 known as Gram positive bacteria, pesticide tolerant bacteria identified as Serratia marcescens which is a Gram negative bacteria group and may cause pathogenic.

ANTIMICROBIAL ACTIVITY OF A MONOALKYL BENZENE SULPHONATE COMPLEX

1962 ◽  
Vol 8 (5) ◽  
pp. 621-628 ◽  
Author(s):  
W. A. Taber ◽  
B. B. Wiley
Keyword(s):  
Active Component ◽  
Fusarium Species ◽  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Fusarium Spp ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Household Detergent ◽  
Fungi Imperfecti

The antimicrobial activities of a branched, monoalkyl benzene sulphonate complex (ABS), the active component of a commercial liquid household detergent, and of the detergent have been investigated. Cultures of dermatophytes, Candida albicans, saprophytic phycomycetes, ascomycetes, fungi imperfecti, Gram-positive and Gram-negative bacteria were tested. Only the Fusarium species and the Gram-negative bacteria were not inhibited by a concentration of 0.1 ml of the detergent/50 ml medium. Microgram quantities of ABS inhibited the Gram-positive bacteria and the fungi except Fusarium spp. ABS was lethal in microgram quantities, the effect being detectable within 30 minutes. Inhibition of exogenous respiration of glucose by C. albicans began upon contact and was complete within 50 minutes. A linear and biodegradable ABS was more active than the branched form against C. albicans.

Review of the genus Phormidesmis (Cyanobacteria) based on environmental, morphological, and molecular data with description of a new genus Leptodesmis

Phytotaxa ◽  
2019 ◽  
Vol 395 (1) ◽  
pp. 1-16 ◽  
Author(s):  
LENKA RAABOVÁ ◽  
LUBOMIR KOVACIK ◽  
JOSEF ELSTER ◽  
OTAKAR STRUNECKÝ
Keyword(s):  
Molecular Data ◽  
Morphological Properties ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Filamentous Cyanobacteria ◽  
Wide Range ◽  
Significant Divergence ◽  
Taxonomic Methods ◽  
Morphological And Molecular Data ◽  
Definition Of

Very thin filamentous cyanobacteria are ubiquitous in a wide range of environments. For many years they were traditionally studied according to their morphological properties only. With the introduction of additional taxonomic methods (cytomorphological analyses, molecular sequencing, exact ecological studies, better data about phytogeographical distribution), traditional genera such as Leptolyngbya and Phormidium were found to be polyphyletic. Phormidesmis belonged to a newly formed genus that was supposed to explain the variability of such very thin simple filamentous cyanobacteria. However, even after definition of Phormidesmis based on distinct cytomorphological and phylogenetic traits, the variability within this genus remained unresolved. Here we analyzed 26 Phormidesmis strains to describe the variability within this genus, classified two new species (P. arctica and P. communis) and transferred Leptolyngbya nigrescens into P. nigrescens. A tabular review of Phormidesmis species is included. The diacritical features of all these species are: width up to 1–4 µm, barrel-shaped cells, which can be shorter or slightly longer than wide, with apparent constrictions at the cross-walls. Our study shows that Phormidesmis is a morphologically and genetically well-defined genus with a global distribution. A newly described genus Leptodesmis has significant morphological similarities both with Phormidesmis and Leptolyngbya, however with intermediate phylogenetic position with significant divergence in 16S rRNA gene. Leptodesmis is cryptic both to Phormidesmis and Leptolyngbya. In the initial part of the life cycle resembles Leptolyngbya, the appearance of older trichomes change to Phormidesmis like morphology.

scholarly journals Characterization, Antimicrobial Properties and Coatings Application of Gellan Gum Oxidized with Hydrogen Peroxide

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 31 ◽  
Author(s):  
Yushuang Lu ◽  
Xiaojian Zhao ◽  
Sheng Fang
Keyword(s):  
Hydrogen Peroxide ◽  
1H Nmr Spectroscopy ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Gellan Gum ◽  
Proton Nuclear Magnetic Resonance ◽  
Carboxyl Groups ◽  
Gram Negative Bacteria ◽  
Gram Positive Bacteria ◽  
H2o2 Oxidation

The effect of hydrogen peroxide (H2O2) oxidation on the physicochemical, gelation and antimicrobial properties of gellan gum was studied. The oxidized gellan gum (OGG) was characterized by measuring the carboxyl/carbonyl group contents, Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H-NMR) spectroscopy. The H2O2 oxidation resulted in a large increase in the carboxyl groups in gellan gum. The OGG lost gelation ability by oxidation even in the presence of metal ions. The antimicrobial activities of the OGG against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli), and fungal (Aspergillus niger) were tested. The OGG could inhibit the growth of both bacteria and fungal, and the activity was improved with an increase in the oxidation level. Finally, the application of the OGG as an active coatings material to extend the storage of apples was tested.

scholarly journals Inhibitory Spectra and Modes of Antimicrobial Action of Gallotannins from Mango Kernels (Mangifera indicaL.)

2011 ◽  
Vol 77 (7) ◽  
pp. 2215-2223 ◽  
Author(s):  
Christina Engels ◽  
Andreas Schieber ◽  
Michael G. Gänzle
Keyword(s):  
Staphylococcus Aureus ◽  
Wild Type Strain ◽  
Antimicrobial Activities ◽  
Resistance Mechanisms ◽  
Gram Negative Bacteria ◽  
Wild Type ◽  
Gram Positive Bacteria ◽  
Food Borne ◽  
Membrane Bound ◽  
Bacteria And Fungi

ABSTRACTThis study investigated the antimicrobial activities and modes of action of penta-, hexa-, hepta-, octa-, nona-, and deca-O-galloylglucose (gallotannins) isolated from mango kernels. The MICs and minimum bactericidal concentrations (MBCs) against food-borne bacteria and fungi were determined using a critical dilution assay. Gram-positive bacteria were generally more susceptible to gallotannins than were Gram-negative bacteria. The MICs of gallotannins againstBacillus subtilis,Bacillus cereus,Clostridium botulinum,Campylobacter jejuni,Listeria monocytogenes, andStaphylococcus aureuswere 0.2 g liter−1or less; enterotoxigenicEscherichia coliandSalmonella entericawere inhibited by 0.5 to 1 g liter−1, and lactic acid bacteria were resistant. The use of lipopolysaccharide mutants ofS. entericaindicated that the outer membrane confers resistance toward gallotannins. Supplementation of LB medium with iron eliminated the inhibitory activity of gallotannins againstStaphylococcus aureus, and siderophore-deficient mutants ofS. entericawere less resistant toward gallotannins than was the wild-type strain. Hepta-O-galloylglucose sensitizedLactobacillus plantarumTMW1.460 to hop extract, indicating inactivation of hop resistance mechanisms, e.g., the multidrug resistance (MDR) transporter HorA. Carbohydrate metabolism ofLactococcus lactisMG1363, a conditionally respiring organism, was influenced by hepta-O-galloylglucose when grown under aerobic conditions and in the presence of heme but not under anaerobic conditions, indicating that gallotannins influence the respiratory chain. In conclusion, the inhibitory activities of gallotannins are attributable to their strong affinity for iron and likely additionally relate to the inactivation of membrane-bound proteins.

scholarly journals Kribbella aluminosa sp. nov., isolated from a medieval alum slate mine

2007 ◽  
Vol 57 (9) ◽  
pp. 1943-1947 ◽  
Author(s):  
Marc René Carlsohn ◽  
Ingrid Groth ◽  
Cathrin Spröer ◽  
Barbara Schütze ◽  
Hans-Peter Saluz ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Fatty Acid Profiles ◽  
16S Rrna Gene Sequences ◽  
Wide Range ◽  
Low Levels ◽  
Type Strains

Three actinomycetes (strains HKI 0478T, HKI 0479 and HKI 0480) isolated from the surfaces of rocks in the Feengrotten medieval alum slate mine (Thuringia, Germany) were examined in a polyphasic taxonomic study. The following morphological and chemotaxonomic features supported their classification as members of the genus Kribbella: the presence of ll-diaminopimelic acid in the cell-wall peptidoglycan; glucose together with minor amounts of mannose and ribose as the whole-cell sugars; polar lipids comprising phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospho- and glycolipids; fatty acid profiles characterized by the predominance of anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 9-methyl; and the presence of MK-9(H4) as the main menaquinone. The isolates had almost identical 16S rRNA gene sequences (99.9–100 %) and were most closely related to the type strains of Kribbella jejuensis (98.9 % sequence similarity), Kribbella swartbergensis and Kribbella solani (both 98.8 %). A wide range of genotypic and phenotypic markers as well as the low levels of DNA–DNA relatedness between strain HKI 0478T and the type strains of K. jejuensis (41.3 %), K. swartbergensis (18.6 %) and K. solani (14.2 %) distinguished the novel strains from their closest phylogenetic neighbours. On the basis of these results, strain HKI 0478T represents a novel member of the genus Kribbella, for which the name Kribbella aluminosa sp. nov. is proposed. The type strain is HKI 0478T (=DSM 18824T =JCM 14599T).

scholarly journals KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO

2018 ◽  
Vol 17 (2) ◽  
pp. 105
Author(s):  
Agus Eko Prasetyo ◽  
Kikin Hamzah Mutaqin ◽  
Giyanto .
Keyword(s):  
Nested Pcr ◽  
In Silico ◽  
Bacterial Species ◽  
Rflp Analysis ◽  
Wilt Disease ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Gram Positive Bacteria ◽  
Pcr Method

Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.

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