Effects of cold stress, alprazolam and phytomedicine in combination with stress on blood glucose and haematogical parameter of the male albino rat

2020 ◽  
Vol 22 ◽  
pp. 37-44
Author(s):  
Piyasi Bhattacharjee ◽  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Cold Stress ◽  
Glucose Level ◽  
Withania Somnifera ◽  
Treated Group ◽  
Control Group ◽  
Root Extract ◽  
Albino Rat

The present study conducted to investigate the haematological changes and changes of blood glucose level in male albino rat due to cold stress. In this experiment normal 12:12 light dark phases were maintained for all the groups. Control group was kept at normal room temperature (22 ± 1). A (4°C), B in (0°C), C (4°C and 0.30 mg alprazolam / kg body weight /animal), D (0°C and 0.30 mg alprazolam/ kg body weight/ animal. E2 group was treated with (4°C and 1000 mg/kg body weight methanolic extract of Withania somnifera root extract /animal). F2 group was treated with (0°C and 1000 mg/kg body weight methanolic root extract of Withania somnifera / animal). The blood glucose level was significantly increased in stressed rats compared to the control animals. The results were also consistent with the exposure to the stress and chronic restraint stress. Action of Alprazolam over cold stress treated group significantly reduced the blood glucose level. Whereas methanolic root extract of Withania somnifera in low and high doses also showed significant effects to the control anxiety like effects on blood glucose level. Alprazolam + different stress treated groups in different experiment at conditions show significant changes in its haematological parameters in comparison to the stress treated group. Whereas herbal medicine (i.e., methanolic root extract of Withania somnifera) when applied to different stress treated group showed more significant result, compared to the Alprazolam+ different stress treated groups. The positive safe anti stress effects of the herbal plant medicine prove that the tribal medicines have the potentiality to act effectively and can be used as safe medicine for antistress purposes.

Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin

2011 ◽  
Vol 9 (1) ◽  
pp. 12-16
Author(s):  
RIZKY OCKTARINI ◽  
DIDING HERI PRASETYO ◽  
IPOP SJARIFAH
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
T Test ◽  
Herbal Extract ◽  
Control Group ◽  
Buffer Solution ◽  
Control Group Design ◽  
Post Test

Ocktarini R, Prasetyo DH, Sjarifah I. 2011. Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin. Biofarmasi 9: 12-16. This study was conducted to determine the effect of herbal extract of anting-anting (Acalypha australis L.) on the decrease of blood glucose level of Balb/C mice induced with Streptozotocin. This research was an experimental laboratory with pre and post-test control group design, conducted in Laboratory of Biochemistry and Histology, Faculty of Medicine, Sebelas Maret University, Surakarta. The test animals used were 16 male mice, about 4-6 weeks of age with approximately 20-30 grams induced with Streptozotocin in 0.02 M citric buffer solution, and a dose of 65 mg/kg body weight. Furthermore, the mice were divided into two groups (Metformin in a dose of 1.3 mg/mice/day and herbal extract of anting-anting 1000 mg/kg BB/day) randomly, each group consisted of 8 mice. All mice were observed for GDS level after 14 days treatment period. The data obtained was processed by using the Independent-Samples T-test statistical test (unpaired t-test) with SPSS for Microsoft Windows Release 18.0. Mean of decreased GDS levels on Metformin group vs herbal extract of anting-anting (145.87 mg/dl vs 144.62 mg/dl, p=0.965). The treatment of herbal extract of anting-anting in a dose of 1000 mg/kg body weight/day could decrease blood glucose level of Balb/C mice induced with Streptozotocin that equal with Metformin.

scholarly journals Effects of ginger juice on blood glucose in alloxan induced diabetes mellitus in rats

2015 ◽  
Vol 23 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Selima Sultana ◽  
Md Ismail Khan ◽  
Hasanur Rahman ◽  
Abu Sadat Mohammad Nurunnabi ◽  
Rokhsana Dil Afroz
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Zingiber Officinale ◽  
Control Group ◽  
Fasting Blood Glucose Level ◽  
Medical College ◽  
Group D

Objective: To find out the effects of ginger (Zingiber officinale) juice blood glucose in alloxan induced-diabetes mellitus in rats. Methods: This experimental study was done in the Department of Pharmacology & Therapeutics, Dhaka Medical College, Dhaka, in collaboration with the Departments of Pathology, Ibrahim Medical College, Dhaka between January and December 2009. This experimental animal study was divided into two parts, which were Experiment-1 and Experiment-2. Experiment 1 comprises of 12 rats and divided into 2 groups each group having 6 rats. Rats of group-A was non-diabetic normal control group and group-B was fed with ginger (Zingiber officinale) in a dose of 4ml/kg body weight orally through Ryle’s tube. Experiment-2 comprised of 12 rats divided into 2 groups each containing 6 rats labeled as group C, group D. Rats of groups C administered alloxan 150 mg/kg intraperitoneally on the 2nd day of the study. Rats of group D were administered alloxan 150mg /kg intraperitoneally and ginger (4ml/kg of body weight orally) on the 2nd day of the study. Results: The fasting blood glucose level at day 12 in the rats treated with ginger (Zingiber officinale) 4 ml /kg body weight orally daily for 12 days showed reduction in fasting blood glucose level as compared to control group, but not significant, which indicates that ginger has no effect in lowering blood glucose of normal rats. The fasting blood glucose levels at day 12 in the rats of group D (treated with ginger and alloxan) showed highly significant reduction in fasting blood glucose level as compared to diabetic control group (p<0.002). Conclusion: Consumption of ginger produced a significant antihyperglycemic effect in experimentally induced diabetic rats. DOI: http://dx.doi.org/10.3329/jdmc.v23i1.22687 J Dhaka Medical College, Vol. 23, No.1, April, 2014, Page 14-17

scholarly journals Effects of Ashwagandha (Withania somnifera) Root Extract On Some Serum Liver Marker Enzymes (AST, ALT) In Gentamicin Intoxicated Rats

2012 ◽  
Vol 7 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Nayma Sultana ◽  
Sadia Choudhury Shimmi ◽  
M Tanveer Hossain Parash ◽  
Jesmine Akhtar
Keyword(s):  
Body Weight ◽  
Withania Somnifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Treated Group ◽  
Control Group ◽  
Root Extract ◽  
Marker Enzymes ◽  
Group B ◽  
Experimental Group

Background: Liver is an essential metabolic organ. It can be damaged due to prolonged use and higher doses of drugs, exposure to some chemicals, toxins, or infectious agents. Herbal plants as ashwagandha (Withania somnifera) may have free radical scavenging activity thereby can be used for the prevention and treatment of liver damage.Objective: To observe the effect of ashwagandha (Withania somnifera) root extract on gentamicin induced changes of some liver marker enzymes e,g serum aspartate amino transferase (AST ) and alanine amino transferase (ALT) in Wistar albino rats.Methods: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st July 2010 to 30th June 2011. A total number of 35 Wistar albino rats, aged 90 to 120 days, weighing between 150 to 200 grams were selected for the study. After acclimatization for 14 days, they were divided into control group (Group A) and experimental group (Group B). Control group was again subdivided into group A1 (baseline control, consisted of 10 rats) and group A2 (gentamicin treated control group, consisted of 10 rats). Again, experimental group (Group B-ashwagandha pretreated and gentamicin treated group) consisted of 15 rats. All groups of animals received basal diet for 22 consecutive days. In addition to this, group A2 also received gentamicin subcutaneously (100mg /kg body weight/day) for the last eight (15th to 22nd day) consecutive days. Again, group B received ashwagandha root extract (500mg/kg body weight/day, orally) for 22 consecutive days and gentamicin subcutaneously (100mg/kg body weight /day) for last eight (15th to 22nd day) days. All the animals were sacrificed on 23rd day. Then blood and liver samples were collected. For assessment of liver function, serum AST, ALT and bilirubin levels were estimated. All these tests were done by standard Laboratory technique. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable.Results: The mean serum levels of AST and ALT were significantly (p<0.001) higher in gentamicin treated control group and in ashwagandha pretreated and gentamicin treated group in comparison to those of baseline control group.. Again, these levels were significantly (p<0.001) lower in ashwagandha pretreated and gentamicin treated group than those of gentamicin treated control group.Conclusion: Ashwagandha (Withania somnifera) root extract restored serum AST, ALT towards normal levels in gentamicin intoxicated rats which may be due to its free radical scavenging activity. Therefore it may have hepatoprotective effect. DOI: http://dx.doi.org/10.3329/jbsp.v7i1.11152 J Bangladesh Soc Physiol. 2012, June; 7(1): 1-7

Effects of Ashwagandha (Withania somnifera) Root Extract Against Gentamicin Induced Changes of Serum Electrolytes in Rats

2012 ◽  
Vol 7 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Sadia Choudhury Shimmi ◽  
Nasim Jahan ◽  
Nayma Sultana
Keyword(s):  
Body Weight ◽  
Withania Somnifera ◽  
Chloride Ion ◽  
Treated Group ◽  
Control Group ◽  
Root Extract ◽  
Serum Electrolytes ◽  
Kidney Weight ◽  
Significant Difference ◽  
Experimental Group

Background: Regulation of electrolytes and body fluids are essential for maintaining the body homeostasis. Kidney plays an important role for these regulations. Higher doses of drugs, toxins, infectious agents, chemicals etc. can causes kidney damage and ultimately electrolytes disturbances can be occurred. Ashwagandha (Withania somnifera) is an herbal plant may have some role on serum electrolytes balance.Objective: To observe the effects of ashwagandha (Withania somnifera) root on serum electrolytes against gentamicin induced nephrotoxicity in Wistar albino rats.Methods: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College SSMC), Dhaka from 1st July 2010 to 30th June 2011. A total number of 35 Wistar albino rats, age from 90 to 120 days, weighing between 150 to 200 grams were selected for the study. After acclimatization for 14 days, they were divided into control group and experimental group. Control group was again subdivided into baseline control, (10 rats) and gentamicin treated control group, (10 rats). Again, experimental group (gentamicin treated group after ashwagandha treatment) consisted of 15 rats. All groups of animals received basal diet for 22 consecutive days. In addition to this, gentamicin treated control group also received gentamicin subcutaneously (100mg /kg body weight/day) for the last eight (15th to 22nd day) consecutive days. Again, gentamicin treated group after ashwagandha treatment received ashwagandha root extract (500mg/kg body weight/day, orally) for 22 consecutive days and gentamicin subcutaneously (100mg/kg body weight /day) for last eight (15th to 22nd day) days. All the animals were sacrificed on 23rd day. Then blood samples were collected and kidney weight was measured. For assessment of kidney function, some serum electrolyte levels e,g. serum sodium, potassium and chloride ion levels were estimated by ion selective electrode (ISE) electrolyte auto analyzer method, by using Biolyte 2000 auto analyzer. However, body weight and kidney weight of the animals were measured to assess the nephrotoxicity in these groups of animals. All these tests were done in the laboratory of Department of Physiology and Biochemistry, SSMC. Statistical analysis was done by one way ANOVA and Bonferroni tests as applicable.Results: The serum sodium and chloride ion levels were almost similar in all the groups and the differences were not statistically significant. The mean serum levels of potassium ion were significantly (p<0.001) lower in gentamicin treated group and (p<0.05) in gentamicin treated group after ashwagandha treatment in comparison to that of baseline control group. But this level of gentamicin treated group after ashwagandha treatment was significantly (p<0.01) higher than that of gentamicin treated group. Initial body weight was almost similar and no significant difference of this value was observed among the groups. Whereas, the final body weight was significantly (p<0.001) lower in gentamicin treated control group and in gentamicin treated group after ashwagandha treatment than that of baseline  control group. Again this level of gentamicin treated group after ashwagandha treatment was significantly (p<0.05) higher in comparison to that of gentamicin treated control group. The kidney weight was significantly (p<0.01) higher in gentamicin treated control group when compared to that of baseline control and gentamicin treated group after ashwagandha treatment. Whereas, kidney weight of gentamicin treated group after ashwagandha treatment and of baseline control group was almost similar and showed no statistically significant difference of this value between this two groups.Conclusion: Ashwagandha (Withania somnifera) root extract may have some role in maintaining some of the serum electrolyte levels within normal limit, which indicates its nephroprotective effects against gentamicin induced toxicity. DOI: http://dx.doi.org/10.3329/jbsp.v7i1.11157 J Bangladesh Soc Physiol. 2012, June; 7(1): 29-35

scholarly journals The effect of Averrhoa bilimbi stem extract on the blood glucose level of white rats induced by alloxan

2017 ◽  
Vol 14 (2) ◽  
pp. 56-62
Author(s):  
CAHYANING GUSTI AGRIANI ◽  
KISRINI KISRINI ◽  
RUBEN DHARMAWAN
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Positive Control ◽  
Drug Group ◽  
Positive Control Group ◽  
Control Group ◽  
White Rats ◽  
Stem Extract

Agriani CN, Kisrini, Dharmawan R. 2016. The effect of Averrhoa bilimbi stem extract on the blood glucose level of white rats induced by alloxan. Biofarmasi 14: 56-62. The purpose of this research is to find out the effect of belimbing wuluh stem (Averrhoa bilimbi L.) extract due to the blood glucose level in white rats (Rattus norvegicus) induced by alloxan. This research is a laboratory experiment using experimental control group pre-post test design. Subjects of this research are 25 white male rats (Rattus norvegicus), 2 months of age and 200 g of body weight. Subjects were divided into five groups. All groups were induced by 25 mg/200 g body weight/day of alloxan for three days. On the day of fourth, blood sample was collected from orbital vein of white rats for the measurement of the first blood glucose level (T1). Furthermore, the positive control group received 12,6 mg/200 g body weight/day of metformin, then the first, second, and third group received of each 25, 50, and 100 mg/200 g body weight/day of belimbing wuluh stem extract. On the fifteenth day of treatment, blood samples were collected again from the orbital vein of white rats for the measurement of the second blood glucose level (T2). The measurement of blood glucose was using spectrophotometer with the Glucose GOD PAP method. Then the data were analyzed by using One way ANOVA. Blood glucose rate after induction has subtracted from blood glucose rate after drug given. After that the data would be change in to mean data that represents as: negative control group = 24,4; positive control group 103,4; first given drug group = 83,4; second given drug group = 102,4; and third given drug group = 102,2 (in mg/100 mL unit). The statistical analysis by using One way ANOVA shows a significant difference in blood glucose level among the five groups of treatment with p-value = 0,000. Post Hoc Test shows that the first, second, and third group have the same effectively as the positive control group to decrease the blood glucose level with p > 0,05.

scholarly journals Effect of Ashwagandha (Withania Somnifera) Root Extract Against Gentamicin Induced Changes of Serum Urea and Creatinine Levels in Rats

1970 ◽  
Vol 6 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Sadia Choudhury Shimmi ◽  
Nasim Jahan ◽  
Nayma Sultana
Keyword(s):  
Body Weight ◽  
Withania Somnifera ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Serum Urea ◽  
Wistar Albino Rats ◽  
Group B ◽  
Experimental Group

Background: Kidney is an important excretory organ. Its damage can be occurred due to prolonged use and higher doses of drugs, exposure to some chemicals, toxins, or infectious agents. Herbal plants as Ashwagandha (Withania somnifera) may have free radical scavenging activity thereby can be used for the prevention and treatment of kidney damage. Objective: To observe the nephroprotective effect of Ashwagandha (Withania somnifera) root against gentamicin induced nephrotoxicity in Wistar albino rats. Methods: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st July 2010 to 30th June 2011. A total number of 35 Wistar albino rats, age ranged from 90 to 120 days, weighing between 150 to 200 grams were included in this study. After acclimatization for 14 days, they were divided into control group (Group A) and experimental group (Group B). Control group was again subdivided into group A1 (baseline control, consisted of 10 rats) and group A2 (gentamicin treated control group, consisted of 10 rats). Again, experimental group (Group B- Ashwagandha pretreated and gentamicin treated group) consisted of 15 rats. All groups of animals received basal diet for 22 consecutive days. In addition to this, group A2 also received gentamicin subcutaneously (100mg /kg body weight/day) for the last eight (15th to 22nd day) consecutive days. Again, group B received ashwagandha root extract (500mg/kg body weight/ day; orally) for 22 consecutive days and gentamicin subcutaneously (100mg/kg body weight /day) for last eight (15th to 22nd day) days. All the animals were sacrificed on 23rd day. Then blood and kidney sample were collected. Estimation of serum urea, creatinine levels were done by using standard Laboratory kits. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable. Results: The mean serum urea, creatinine levels were significantly (p<0.001) higher in gentamicin treated control group in comparison to those of baseline control. Again, these levels were significantly (p<0.01) lower in ashwagandha pretreated and gentamicin treated group (experimental group) when compared to those of gentamicin treated group (control). Conclusion: Ashwagandha (Withania somnifera) root may have some nephroprotective effect against gentamicin induced nephrotoxicity. DOI: http://dx.doi.org/10.3329/jbsp.v6i2.9756 JBSP 2011 6(2): 84-89

scholarly journals The effect of daun sendok (Plantago major) extract on the blood glucose level of Balb/C mice induced by streptozotocin

2010 ◽  
Vol 8 (2) ◽  
pp. 66-71
Author(s):  
PUTRI SATRIANY ◽  
R. P. ANDRI PUTRANTO ◽  
IPOP SYARIFAH
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Simple Random Sampling ◽  
Control Group ◽  
Plantago Major ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

Satriany P, Putranto RPA, Syarifah I. 2010. The effect of daun sendok (Plantago major) extract on the blood glucose level of Balb/C mice induced by streptozotocin. Biofarmasi 8: 66-71. This research aimed to determine the effect of daun sendok (Plantago major L.) extract on the blood glucose level of Balb/C mice induced by streptozotocin. This study was a laboratory experimental pre and post-test control group design. The subjects used were 16 male mice. Then, mice were induced with streptozotocin by a dose of 65 mg/kg body weight intraperitoneally freshly dissolved in 0.02 M citrate saline buffer. Mice used were mice with blood glucose levels â‰¥200 mg/dL. Furthermore, mice were divided into 2 groups by a simple random sampling. Group 1 was given metformin in a dose of 1.3 mg/mice/day and group 2 was given daun sendok extract in a dose of 1000 mg/kg body weight/day. At the 2nd week, it was measured for the glucose levels from the blood of mice tail by a Blood Glucose Stick Meter (Gluco DrTM). The data obtained were statistically analyzed by independent samples t-test by using SPSS Programme for Microsoft Windows release 17.0. The significance was set at p<0.05. The difference of average blood glucose levels pre vs post experiment, metformin group was -145.87 mg/dL and daun sendok group was -85.00 mg/dL (p=0.024). The experiment results showed that daun sendok extract in a dose of 1000 mg/kg body weight/day could reduce the blood glucose levels in Balb/C mice induced by streptozotocin.

scholarly journals Pemberian ekstrak air buah sawo (Manilkara zapota L.) menurunkan kadar glukosa darah tikus (rattus norvegicus) diabetes mellitus

2016 ◽  
Vol 3 (3) ◽  
pp. 180
Author(s):  
Effatul Afifah
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

<p><strong>ABSTRACT</strong><br /><br /><em><strong>Background</strong>: Diabetes mellitus (DM) is a chronic metabolic problem disorder characterized by hyperglicemia which is caused by insulin deficiency produced by β-pancreas cells, thus causing abnormalities of carbohydrate, protein, and fat metabolism, and tend to cause complications. </em></p><p><em><strong>Objectives</strong>: To know the effect of sapodilla extract water on blood glucose level of diabetic induce mice. </em></p><p><em><strong>Methods</strong>: This was an experimental study with pre-post control group design. Sapodilla extract water (EABS) was fed to group of mice with alloxan diabetes induction. Twenty four DM induced mice were separated into 4 groups, e.g. control without and with medication of glibenclamide, EABS 1 (treated with 3.6 mL/200 g body weight), and EABS 2 (treated with 7.2 mL/200 g body weight). Mice were then measured for their blood glucose level at the day of 3, 14, and 30. </em></p><p><em><strong>Results</strong>: EABS 1 and EABS 2 decreased blood glucose levels at week of 1st, 2nd, and 3rd after induction. The greatest reduction was shown by EABS 2 at the 3rd week. EABS decreased blood glucose level of mice induced DM and signifi cantly shown at glibenclamide group, followed by EABS 2 and EABS 1.</em></p><p><em><strong>Conclusions</strong>: EABS reduced blood glucose levels of diabetic mice and signifi cantly shown for glibenclamide group, followed by EABS 2 and EABS 1.</em></p><p><strong>KEYWORDS</strong>:<em> diabetes mellitus, sapodilla extract water, blood glucose</em></p><p><br /><strong>ABSTRAK</strong></p><p><em><strong>Latar belakang</strong>: Diabetes mellitus (DM) merupakan penyakit gangguan metabolik kronis yang ditandai dengan hiperglikemia yang disebabkan oleh kekurangan insulin yang dihasilkan oleh sel β-pankreas sehingga menimbulkan kelainan metabolisme karbohidrat, protein dan lemak, dan cenderung menimbulkan komplikasi.</em></p><p><em><strong>Tujuan</strong>: Mengetahui efek pemberian ekstrak buah air sawo terhadap kadar glukosa darah tikus yang diinduksi DM.</em></p><p><em><strong>Metode</strong>: Penelitian ini merupakan penelitian eksperimental murni dengan rancangan pre-post test control group design dengan memberikan intervensi ekstrak buah air sawo (EABS) pada kelompok tikus yang diberikan induksi DM menggunakan aloksan. Sebanyak 24 tikus yang diinduksi DM dikelompokkan ke dalam 4 perlakuan, antara lain kontrol tanpa dan dengan pengobatan glienclamide, EABS 1 (diberi 3,6 mL/200 g berat badan), dan EABS 2 (7,2 mL/200 g berat badan). Tikus diukur kadar gula darahnya pada hari ke-3, 14, dan 30. </em></p><p><em><strong>Hasil</strong>: EABS mampu menurunkan kadar glukosa darah pada tikus, baik pada kelompok EABS 1 maupun EABS 2 pada minggu ke-1, ke-2, dan ke-3 setelah induksi. Hasil penurunan kadar glukosa yang paling besar terjadi pada kelompok EABS 2 pada minggu ke-3 perlakuan. EABS dapat menurunkan kadar glukosa darah tikus yang diinduksi DM dan paling signifi kan terjadi pada kelompok glibenclamide diikuti oleh EABS 2 dan EABS 1.</em></p><p><em><strong>Kesimpulan</strong>: EABS mampu menurunkan kadar glukosa darah pada tikus yang diinduksi alloxan.</em></p><p><strong>KATA KUNCI</strong><em>: diabetes mellitus, ekstrak air buah sawo, glukosa darah</em></p>

scholarly journals Uji efek air perasan albedo semangka kuning (Citrilus lanatus (Thunb.) Matsum. & Nakai) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Savitri Muhammad Nur ◽  
Henoch Awaloei ◽  
Jane Wuisan
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
White Layer ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract: Indonesian has various natural resources, second as largest after Brazil. One of the traditional medicine that has been used is watermelon. The part of watermelon that is known to be used as medicine is its white layer between the peel and the fruit, albedo. This study aimed to determine the effect of yellow watermelon albedo juice on blood glucose levels in wistar rats induced by alloxan. Subjects consisted of 18 male Wistar rats divided into 6 groups as follows: a negative control group, 3 groups of hyperglycemic rats induced with alloxan 120 mg/kg body weight; and 2 groups given only yellow watermelon albedo juice. Hyperglycemic rats were given yellow watermelon albedo with dose of 9 g/kg body weight and 4,5 g/kg body weight; positive control group was given novomix; and two other groups were given only yellow watermelon albedo juice. Blood glucose levels of each rat were evaluated on day 1, day 2, and day 3 before the treatment after 6 hours, 12 hours, 18 hours, 24 hours. The results showed that administration of yellow watermelon albedo juice 9 g/kg body weight of Wistar rats reduced the blood glucose level until 126.67 mg/dl, meanwhile the dose 4.5 g/kg body weight reduced the blood glucose level until 173.67 mg/dl. Conclusion: Administration of yellow watermelon albedo juice of 9 g/kg body weight and 4.5 g/kg body weight could reduce blood glucose levels of hyperglycemic Wistar rats induced by alloxan. Keywords: yellow watermelon albedo, hyperglycemia, blood glucose level Abstrak: Indonesia merupakan negara yang memiliki keanekaragaman hayati terkaya kedua didunia setelah Brazil. Salah satu obat tradisional yang digunakan ialah buah semangka. Bagian dari semangka yang digunakan ialah lapisan yang berwarna putih yakni antara kulit dan daging buah semangka disebut albedo. Penelitian ini bertujuan untuk menguji pengaruh pemberian air perasan albedo semangka kuning terhadap kadar glukosa darah pada tikus wistar yang diinduksi aloksan. Subjek penelitian yaitu 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok, yaitu: kelompok kontrol negatif; 3 kelompok tikus Wistar hiperglikemik yang diinduksi aloksan dengan dosis 120 mg/kg BB; dan 2 kelompok tikus Wistar yang hanya diberikan albedo semangka kuning. Kelompok tikus yang hiperglikemik diberi albedo semangka kuning dengan dosis 9 g/kg BB dan 4,5 g/kg BB; kelompok kontrol positif diberikan novomix; dan 2 kelompok hanya diberikan albedo semangka kuning. Pemeriksaan kadar glukosa darah dari semua kelompok tikus Wistar dilakukan pada hari ke-1, hari ke-2, dan hari ke-3 pada jam ke 0, 6, 12, 18, dan 24. Hasil penelitian memperlihatkan pemberian air perasan albedo semangka kuning dosis 9 g/kg BB dapat menurunkan kadar glukosa darah sebanyak 126,67 mg/dl sedangkan dosis 4,5 g/kg BB sebanyak 173,67 mg/dl. Simpulan: Air perasan albedo semangka kuning dengan dosis 9 g/kg BB dan 4,5 g/kg BB dapat menurunkan kadar glukosa darah tikus Wistar hiperglikemik yang diinduksi aloksan.Kata kunci: albedo semangka kuning, hipeglikemi, kadar glukosa darah

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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