The effect of Averrhoa bilimbi stem extract on the blood glucose level of white rats induced by alloxan

Agriani CN, Kisrini, Dharmawan R. 2016. The effect of Averrhoa bilimbi stem extract on the blood glucose level of white rats induced by alloxan. Biofarmasi 14: 56-62. The purpose of this research is to find out the effect of belimbing wuluh stem (Averrhoa bilimbi L.) extract due to the blood glucose level in white rats (Rattus norvegicus) induced by alloxan. This research is a laboratory experiment using experimental control group pre-post test design. Subjects of this research are 25 white male rats (Rattus norvegicus), 2 months of age and 200 g of body weight. Subjects were divided into five groups. All groups were induced by 25 mg/200 g body weight/day of alloxan for three days. On the day of fourth, blood sample was collected from orbital vein of white rats for the measurement of the first blood glucose level (T1). Furthermore, the positive control group received 12,6 mg/200 g body weight/day of metformin, then the first, second, and third group received of each 25, 50, and 100 mg/200 g body weight/day of belimbing wuluh stem extract. On the fifteenth day of treatment, blood samples were collected again from the orbital vein of white rats for the measurement of the second blood glucose level (T2). The measurement of blood glucose was using spectrophotometer with the Glucose GOD PAP method. Then the data were analyzed by using One way ANOVA. Blood glucose rate after induction has subtracted from blood glucose rate after drug given. After that the data would be change in to mean data that represents as: negative control group = 24,4; positive control group 103,4; first given drug group = 83,4; second given drug group = 102,4; and third given drug group = 102,2 (in mg/100 mL unit). The statistical analysis by using One way ANOVA shows a significant difference in blood glucose level among the five groups of treatment with p-value = 0,000. Post Hoc Test shows that the first, second, and third group have the same effectively as the positive control group to decrease the blood glucose level with p > 0,05.

Download Full-text

Effects of cold stress, alprazolam and phytomedicine in combination with stress on blood glucose and haematogical parameter of the male albino rat

INTERNATIONAL JOURNAL OF EXPERIMENTAL RESEARCH AND REVIEW ◽

10.52756/ijerr.2020.v22.005 ◽

2020 ◽

Vol 22 ◽

pp. 37-44

Author(s):

Piyasi Bhattacharjee ◽

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Cold Stress ◽

Glucose Level ◽

Withania Somnifera ◽

Treated Group ◽

Control Group ◽

Root Extract ◽

Albino Rat

The present study conducted to investigate the haematological changes and changes of blood glucose level in male albino rat due to cold stress. In this experiment normal 12:12 light dark phases were maintained for all the groups. Control group was kept at normal room temperature (22 ± 1). A (4°C), B in (0°C), C (4°C and 0.30 mg alprazolam / kg body weight /animal), D (0°C and 0.30 mg alprazolam/ kg body weight/ animal. E2 group was treated with (4°C and 1000 mg/kg body weight methanolic extract of Withania somnifera root extract /animal). F2 group was treated with (0°C and 1000 mg/kg body weight methanolic root extract of Withania somnifera / animal). The blood glucose level was significantly increased in stressed rats compared to the control animals. The results were also consistent with the exposure to the stress and chronic restraint stress. Action of Alprazolam over cold stress treated group significantly reduced the blood glucose level. Whereas methanolic root extract of Withania somnifera in low and high doses also showed significant effects to the control anxiety like effects on blood glucose level. Alprazolam + different stress treated groups in different experiment at conditions show significant changes in its haematological parameters in comparison to the stress treated group. Whereas herbal medicine (i.e., methanolic root extract of Withania somnifera) when applied to different stress treated group showed more significant result, compared to the Alprazolam+ different stress treated groups. The positive safe anti stress effects of the herbal plant medicine prove that the tribal medicines have the potentiality to act effectively and can be used as safe medicine for antistress purposes.

Download Full-text

Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f090103 ◽

2011 ◽

Vol 9 (1) ◽

pp. 12-16

Author(s):

RIZKY OCKTARINI ◽

DIDING HERI PRASETYO ◽

IPOP SJARIFAH

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

T Test ◽

Herbal Extract ◽

Control Group ◽

Buffer Solution ◽

Control Group Design ◽

Post Test

Ocktarini R, Prasetyo DH, Sjarifah I. 2011. Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin. Biofarmasi 9: 12-16. This study was conducted to determine the effect of herbal extract of anting-anting (Acalypha australis L.) on the decrease of blood glucose level of Balb/C mice induced with Streptozotocin. This research was an experimental laboratory with pre and post-test control group design, conducted in Laboratory of Biochemistry and Histology, Faculty of Medicine, Sebelas Maret University, Surakarta. The test animals used were 16 male mice, about 4-6 weeks of age with approximately 20-30 grams induced with Streptozotocin in 0.02 M citric buffer solution, and a dose of 65 mg/kg body weight. Furthermore, the mice were divided into two groups (Metformin in a dose of 1.3 mg/mice/day and herbal extract of anting-anting 1000 mg/kg BB/day) randomly, each group consisted of 8 mice. All mice were observed for GDS level after 14 days treatment period. The data obtained was processed by using the Independent-Samples T-test statistical test (unpaired t-test) with SPSS for Microsoft Windows Release 18.0. Mean of decreased GDS levels on Metformin group vs herbal extract of anting-anting (145.87 mg/dl vs 144.62 mg/dl, p=0.965). The treatment of herbal extract of anting-anting in a dose of 1000 mg/kg body weight/day could decrease blood glucose level of Balb/C mice induced with Streptozotocin that equal with Metformin.

Download Full-text

Antidiabetic activity of musa acuminata colla fruit peel (MACFP) ethanol extract in glucose-induced diabetic rats

MATEC Web of Conferences ◽

10.1051/matecconf/201819707007 ◽

2018 ◽

Vol 197 ◽

pp. 07007

Author(s):

Erza Genatrika ◽

Visda Nila Happy Laksari ◽

Tjiptasurasa Tjiptasurasa

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Ethanol Extract ◽

Positive Control ◽

Diabetic Rats ◽

Musa Acuminata ◽

Antidiabetic Activity ◽

Control Group ◽

Fruit Peel

Diabetes mellitus is a metabolic disorder disease characterized by increased blood glucose levels due to impaired insulin secretion or increased insulin resistance. The study was performed to investigate the blood glucose lowering effect of Musa acuminata Colla fruit peel (MACFP) ethanol extract in experimentally induced diabetic rats. 25 rats were divided into five groups, i.e negative control group (0.5% NaCMC), positive control group (glibenclamide), and treatment group of MACFP ethanol extract with dose 250, 375, and 500 mg/kg body weight (mg/kg BW). The blood sample was taken from the lateral vein of the tail, and then blood glucose level was observed on 0, 30, 60, 90, and 120 minutes. The results provide information that MACFP ethanol extract with dose 250, 375, and 500 mg/kg BW have the same effect with a positive control (glibenclamide) in lowering blood glucose level on diabetic rats (p>0,05). MACFP ethanol extract with dose 500 mg/kg BW had the highest percentage of decrease in blood glucose level (42,62%), followed by doses 375 mg/kg BW (37,26%) and 250 mg/kg BW (24,12%).

Download Full-text

EFEKTIVITAS BUBUK KOPI ROBUSTA FUNGSIONAL DIFORTIFIKASI BUBUK DAUN KERSEN TERHADAP PENURUNAN KADAR GULA DARAH MENCIT DIABETES

Pro Food ◽

10.29303/profood.v6i1.131 ◽

2020 ◽

Vol 6 (1) ◽

pp. 581

Author(s):

Imam Adriansyah ◽

Dody Handito ◽

Rucitra Widyasari

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Positive Control ◽

The Body ◽

Control Group ◽

Diabetic Mice ◽

Robusta Coffee ◽

Coffee Powder ◽

Muntingia Calabura

ABSTRACT The aim of this present study was to determine the effectiveness of fucntional Robusta coffee powder fortified by Muntingia calabura L. leaves to lower blood glucose level in diabetic mice according to the antioxidant activity and total phenolic content, and the effectivness of the chosen ratio of the antidiabetic functional coffee beverage to the body weight and blood glucose level in diabetic mice. This research was conducted in two stages. First, determining the best ratio of the antidiabetic functional coffee beverage using randomized complete design. Second, testing the best ratio to the speciment using the randomized post test-only control group design to perform the chosen ratio of the antidiabetic functional coffee beverage to diabetic mice in seven days treatment. The results showed that the best ratio of the antidiabetic functional coffee beverage was 25% robusta coffee powder and 75% Muntingia calabura L. leaves powder with 88.26% antioxidant activity and 1.05 mg GAE/g sample, and the chosen ratio of the antidiabetic functional coffee beverage proved the activity to reduce the blood glucose level in diabetic mice with the decrease level was 266 mg/dl or 45% effective to reduced the blood glucose level in diabetic mice. This blood glucose reduced activity was not significant to the positive control group given glibenclamid, but it was found significant to the negative control group that given aquades per oral. While the body weight of the diabetic mice given aquades only decreased twice higher than positive control group and antidiabetic functional coffee beverage group. Keywords: blood glucose level, diabetic, Muntingia calabura L. leaves, robusta coffee ABSTRAK Tujuan dari penelitian ini adalah untuk menentukan rasio terbaik antara bubuk kopi robusta dan bubuk daun kersen sebagai minuman fungsional yang difortifikasi bubuk daun kersen terhadap penurunan kadar gula darah mencit diabetes, yang ditinjau dari aktivitas antioksidan dan kadar fenolik total, serta pengaruh rasio terpilih terhadap berat badan dan penurunan kadar gula darah mencit diabetes. Penelitian ini dilakukan dalam dua tahap, yang pertama yaitu penentuan rasio terbaik antara bubuk kopi robusta dan bubuk daun kersen menggunakan metode rancangan acak lengkap (RAL) dengan faktor tunggal berupa rasio bubuk kopi robusta dan bubuk daun kersen. Kedua, yaitu pengujian rasio terbaik pada hewan percobaan menggunakan metode rancangan acak dengan tes akhir dan kelompok kontrol dengan parameter kadar gula darah dan berat badan mencit selama tujuh hari perlakuan. Hasil dari penelitian ini adalah rasio terbaik dari kopi fungsional antidiabetes diperoleh dari rasio 25% bubuk kopi robusta dan 75% bubuk daun kersen dengan aktivitas antioksidan sebesar 88,26% dan kadar fenolik total sebesar 1,05 mg GAE/g bahan. Berdasarkan uji in vivo, produk kopi fungsional antidiabetes mampu menurunkan kadar gula darah mencit diabetes sebesar 266 mg/dl atau sekitar 45%, tidak berbeda nyata dengan perlakuan kontrol positif yang diberikan obat glibenklamid dengan penurunan sebesar 268 mg/dl atau sekitar 47%, namun berbeda nyata dengan kelompok perlakuan kontrol negaif menggunakan aquades yang mengalami peningkatan kadar gula darah sebsar 4,2%. Sedangkan untuk parameter berat badan, bahwa penurunan berat badan mencit kelompok perlakuan kontrol negatif menggunakan aquades mengalami penurunan dua kali lebih besar dibandingkan kelompok kontrol positif dan kelompok produk kopi fungsional. Kata kunci: daun kersen, diabetes, kadar gula darah, kopi robusta

Download Full-text

Uji Aktivitas Antidiabetes Ekstrak Bonggol Nanas (Ananas comusus L.) pada Tikus yang Di induksi Aloksan

Medicra (Journal of Medical Laboratory Science/Technology) ◽

10.21070/medicra.v1i1.1473 ◽

2018 ◽

Vol 1 (1) ◽

pp. 36

Author(s):

Ayu Rochmawati ◽

Syahrul Ardiansyah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Blood Glucose Concentration ◽

Inflammatory Diseases ◽

Positive Control ◽

Negative Control ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Stem Extract

This research aims to knowing about decrease glucose levels in rat induced alloxan with the provision of pineapple stem extract (Ananas comusus L.). Previous studies have shown that bromelin can significantly reduce CD4+ lymphocytes, which are included in inflammatory diseases. One of the inflammatory diseases is diabetes mellitus. Blood glucose levels can be lowered by pineapple extract as it contains bromelin, and bromelin most widely found on the stem. The test animals used white rat strain wistar (Rattus norvegicus L.) with weight is 250-300 gram acclimated for seven days. The research was divided six treatment groups: positive control, negative control, P1 (25% concentration), P2 (50% concentration), P3 (75% concentration), P4 (100% concentration). After treatment for rat in category or sampel was administered pineapple stem extract for 14 days after that experiment status blood glucose level. The results showed that rat had increased (hyperglicemic) after alloxan induced,and also in blood glucose levels in all treatment. the biggest decrease was in P4 is 44 mgdl. The statistic of Kruskal-Wallis test showed that the decrease of rat blood glucose concentration at various concentration of pineapple extract influence signifificantly. According to the result that conclusion is the difference of pineapple extract concentration (25%, 50%, 75%, 100%) influence for decrease blood glucose levels and decreasing blood glucose level after 14 days, but in this time blood glucose not returned as normal conditions.

Download Full-text

Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3490 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Intan Fitri Aprila ◽

M. Nur Salim ◽

Razali Daud ◽

T. Armansyah ◽

Nuzul Asmilia ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Mus Musculus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water ◽

Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

Download Full-text

Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

Smart Medical Journal ◽

10.13057/smj.v1i1.24180 ◽

2018 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Eldaa Putik Bunga Melati ◽

Budiyanti Wiboworini ◽

Briandani Subariyanti

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Rhizopus Sp

Introductions : Controlling of blood glucose would be important to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus).Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicusgrouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/100gramBW, then randomly allocated into 5 group, positive control group (glibenclamide 0.05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0.025gram/100 gramBW/day; 0.050gram/100 gramBW/day; 0.100gram/100gramBW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measuredbefore Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).Results : there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0.001). Post Hoc Test showed a significant difference between all treatment groups and positive control group compared to negative control group (p = 0.001).Conclusion :Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic rats (Rattus norvegicus). Keyword : Tofu fermented, Rhizopus sp., blood glucose level, alloxan, glibenclamide

Download Full-text

Effects of ginger juice on blood glucose in alloxan induced diabetes mellitus in rats

Journal of Dhaka Medical College ◽

10.3329/jdmc.v23i1.22687 ◽

2015 ◽

Vol 23 (1) ◽

pp. 14-17 ◽

Cited By ~ 1

Author(s):

Selima Sultana ◽

Md Ismail Khan ◽

Hasanur Rahman ◽

Abu Sadat Mohammad Nurunnabi ◽

Rokhsana Dil Afroz

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Zingiber Officinale ◽

Control Group ◽

Fasting Blood Glucose Level ◽

Medical College ◽

Group D

Objective: To find out the effects of ginger (Zingiber officinale) juice blood glucose in alloxan induced-diabetes mellitus in rats. Methods: This experimental study was done in the Department of Pharmacology & Therapeutics, Dhaka Medical College, Dhaka, in collaboration with the Departments of Pathology, Ibrahim Medical College, Dhaka between January and December 2009. This experimental animal study was divided into two parts, which were Experiment-1 and Experiment-2. Experiment 1 comprises of 12 rats and divided into 2 groups each group having 6 rats. Rats of group-A was non-diabetic normal control group and group-B was fed with ginger (Zingiber officinale) in a dose of 4ml/kg body weight orally through Ryles tube. Experiment-2 comprised of 12 rats divided into 2 groups each containing 6 rats labeled as group C, group D. Rats of groups C administered alloxan 150 mg/kg intraperitoneally on the 2nd day of the study. Rats of group D were administered alloxan 150mg /kg intraperitoneally and ginger (4ml/kg of body weight orally) on the 2nd day of the study. Results: The fasting blood glucose level at day 12 in the rats treated with ginger (Zingiber officinale) 4 ml /kg body weight orally daily for 12 days showed reduction in fasting blood glucose level as compared to control group, but not significant, which indicates that ginger has no effect in lowering blood glucose of normal rats. The fasting blood glucose levels at day 12 in the rats of group D (treated with ginger and alloxan) showed highly significant reduction in fasting blood glucose level as compared to diabetic control group (p<0.002). Conclusion: Consumption of ginger produced a significant antihyperglycemic effect in experimentally induced diabetic rats. DOI: http://dx.doi.org/10.3329/jdmc.v23i1.22687 J Dhaka Medical College, Vol. 23, No.1, April, 2014, Page 14-17

Download Full-text

Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

Nusantara Bioscience ◽

10.13057/nusbiosci/n090312 ◽

2017 ◽

Vol 9 (3) ◽

pp. 312-317

Author(s):

KARTIAWATI ALIPIN ◽

EMA PURNAMA SARI ◽

MADIHAH MADIHAH ◽

TIA SETIAWATI ◽

NINING RATNINGSIH ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Positive Control ◽

Negative Control ◽

Kidney Damage ◽

Diabetic Rat ◽

Control Group ◽

Male Wistar Rats

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level â‰¥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

Download Full-text