scholarly journals High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico

2017 ◽  
Vol 66 (7) ◽  
pp. 972-980 ◽  
Author(s):  
Magdalena de Jesús Uribe-Beltrán ◽  
Yesmi Patricia Ahumada-Santos ◽  
Sylvia Páz Díaz-Camacho ◽  
Carlos Alberto Eslava-Campos ◽  
Jesús Ernesto Reyes-Valenzuela ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
High Prevalence

scholarly journals Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals

2021 ◽  
Vol 22 (11) ◽  
pp. 5905
Author(s):  
Olivia M. Grünzweil ◽  
Lauren Palmer ◽  
Adriana Cabal ◽  
Michael P. Szostak ◽  
Werner Ruppitsch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Marine Mammals ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Healthcare Sector ◽  
Whole Genome ◽  
E Coli ◽  
High Prevalence ◽  
Lactamase Gene ◽  
Sequence Types

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene blaCMY (n = 51) was the predominant β-lactamase gene. In addition, blaTEM-1 (n = 38), blaSHV-33 (n = 8), blaCTX-M-15 (n = 7), blaOXA-1 (n = 7), blaSHV-11 (n = 3), and blaDHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.

Isolation and Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Foods Sold at Retail Markets in China

2019 ◽  
Vol 83 (3) ◽  
pp. 460-466
Author(s):  
GUANGZHU YANG ◽  
SHUHONG ZHANG ◽  
YUANBIN HUANG ◽  
QINGHUA YE ◽  
JUMEI ZHANG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Shiga Toxin ◽  
Multidrug Resistant ◽  
Food Samples ◽  
High Genetic Diversity ◽  
Retail Markets ◽  
Republic Of China ◽  
Isolation And Characterization ◽  
High Prevalence

ABSTRACT Non-O157 Shiga toxin–producing Escherichia coli (STEC) strains are significant foodborne pathogens that can cause acute diarrhea in humans. This study was conducted to investigate the contamination by non-O157 STEC in different types of food sold at retail markets in the People's Republic of China and to characterize non-O157 STEC strains. From May 2012 to April 2014, 1,200 retail food samples were collected from markets in 24 cities in China. Forty-four non-O157 isolates were recovered from 43 STEC-positive samples. Of the isolates, 22 and 19 carried the stx1 and stx2 genes, respectively, and 3 harbored both stx1 and stx2. stx1a and stx2a were the most prevalent stx subtypes. Other virulence genes, ent, hlyA, astA, eae, espB, iha, subAB, and tia, were commonly detected. Diverse O serogroups were identified among these isolates. Multilocus sequence typing indicated the high genetic diversity of the isolates. Thirty-two sequence types (STs) were identified among the 44 isolates, with ST383 (9.09%), ST134 (6.82%), and ST91 (6.82%) the most prevalent. Nine new STs were found. The isolates had a high prevalence of resistance to cephalothin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, nalidixic acid, streptomycin, and chloramphenicol. Twenty isolates (45.45%) were resistant to at least three antibiotics. This study provides updated surveillance data for non-O157 STEC isolates from foods sold at retail markets. Virulent and multidrug-resistant non-O57 STEC strains were isolated from all types of food. Our findings highlight the need for increased monitoring of non-O157 STEC in retail foods. HIGHLIGHTS

scholarly journals High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran

2014 ◽  
Vol 5 (4) ◽  
pp. 193-198 ◽  
Author(s):  
Mohammad Kargar ◽  
Zahra Mohammadalipour ◽  
Abbas Doosti ◽  
Shahrokh Lorzadeh ◽  
Alireza Japoni-Nejad
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Diarrheagenic Escherichia Coli ◽  
High Prevalence ◽  
Class 1 ◽  
Southwest Of Iran

scholarly journals Antibacterial Activity of Essential Oil of <i>Aeollanthus pubescens</i> on Multidrug Resistant Strains of <i>Salmonella</i> and <i>Escherichia coli</i> Isolated from Laying Hens Farming in Benin

2019 ◽  
Vol 09 (09) ◽  
pp. 804-823
Author(s):  
Aguidissou Oscar Nestor ◽  
Boko Kadoeito Cyrille ◽  
Sessou Philippe ◽  
Yovo Mahudro ◽  
Komagbe S. Gwladys ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Laying Hens ◽  
Multidrug Resistant ◽  
Resistant Strains

scholarly journals Antibacterial Activity of Aqueous Extract of Momordica charantia and Terminalia catappa on Multidrug Resistant Invasive Escherichia coli isolated in Ready-to-Eat (RTE) Foods from Ekiti State

2019 ◽  
Vol 62 (3) ◽  
pp. 183-187
Author(s):  
Ajenifuja Oluwafemi Adeyemi ◽  
Ariyo David Oluwasegun
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Multidrug Resistant ◽  
Momordica Charantia ◽  
Microbial Load ◽  
Dilution Technique ◽  
Smoked Fish ◽  
Terminalia Catappa ◽  
Agar Dilution

  Antibacterial activity of aqueous extract of Momordica charantia and Terminalia catappa was investigated against multidrug resistant invasive Escherichia coli isolated from ready-to-eat foods such as sausage rolls, meat pie, egg roll, doughnut and smoked fish. From the samples tested, smoked fish was found to have the highest microbial load of 1.05x106cfu/mL (55%) followed by meat pie 7.5x105cfu/mL (37%); Sausage roll 7.0x105cfu/mL (36%); and egg roll with 6.5x105cfu/mL (31%). Doughnut was found to have the lowest microbial load of 58x105cfu/mL (20%). The minimum inhibitory concentration (MIC) of the extract determined by agar dilution technique showed that M. charantia and T. catappa had an MIC of 0.5 g/mL and the degree of antibacterial potency of the extracts determined using broth dilution technique showed that at concentration of 0.5 mg/mL, M. charantia and T. catappa had diameter of zone of inhibition of 10.4mm and 11.2 mm, respectively. The observed antibacterial effect of the T. catappa was more pronounced. This investigation depicts positive results by the use of T. catappa extract in drug development for the treatment of infections caused by E. coli isolated from ready-to-eat foods.  

scholarly journals High Prevalence of blaCTX-M-15 Gene among Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates Causing Extraintestinal Infections in Bangladesh

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 796
Author(s):  
Razib Mazumder ◽  
Ahmed Abdullah ◽  
Dilruba Ahmed ◽  
Arif Hussain
Keyword(s):  
Escherichia Coli ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Control Measures ◽  
E Coli ◽  
Infection Control Measures ◽  
Extended Spectrum ◽  
Virulence Potential ◽  
High Prevalence ◽  
High Virulence

The emergence of multidrug-resistant (MDR) Escherichia coli (E. coli) clonal lineages with high virulence potential is alarming. Lack of sufficient data on molecular epidemiology of such pathogens from countries with high infection burden, such as Bangladesh, hinders management and infection control measures. In this study, we assessed the population structure, virulence potential and antimicrobial susceptibility of clinical E. coli isolates from Dhaka, Bangladesh. A high prevalence of MDR (69%) and extended-spectrum β-lactamase production (ESBL) (51%) was found. Most E. coli isolates were susceptible to amikacin (95%), meropenem (94%) and nitrofurantoin (89%) antibiotics. A high prevalence of ST131 (22%) and ST95 (9%) followed by ST69 (4%) and ST73 (3%) was observed. Phylogroups B2 (46%), B1 (16%), D (10%) and F (9%) were prominent. blaCTX-M-15 (52%) and blaNDM-1 (5%) were the most prevalent ESBL and carbapenem resistance genes, respectively. Moreover, the predominant pathotype identified was extraintestinal pathogenic E. coli (ExPEC) (41%) followed by enteric pathogens (11%). In conclusion, our results suggest the transmission of clonal E. coli groups amidst diverse E. coli population that are associated with high virulence potential and MDR phenotype. This is of high concern and mandates more efforts towards molecular surveillance of antimicrobial resistance (AMR) in clinically significant pathogens.

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals Isolation, Identification And Screening Antibacterial Activity from Marine Sponge-Associated Fungi Against Multidrug-Resistant (MDR)Escherichia coli

2017 ◽  
Vol 55 ◽  
pp. 012028 ◽  
Author(s):  
Mada Triandala Sibero ◽  
Aninditia Sabdaningsih ◽  
Olvi Cristianawati ◽  
Handung Nuryadi ◽  
Ocky Karna Radjasa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Marine Sponge ◽  
Multidrug Resistant

scholarly journals High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shahana Ahmed ◽  
Tridip Das ◽  
Md Zohorul Islam ◽  
Ana Herrero-Fresno ◽  
Paritosh Kumar Biswas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Broiler Chickens ◽  
Multidrug Resistant ◽  
Genomic Diversity ◽  
Poultry Production ◽  
E Coli ◽  
Strain Collection ◽  
Antimicrobial Resistance Genes ◽  
High Prevalence

Abstract Colistin is a last-resort antimicrobial used for the treatment of human infections caused by multidrug-resistant Gram-negative bacteria. However, colistin is still widely used in intensive poultry production in Bangladesh. We aimed to investigate the dynamics and genetic diversity of colistin-resistant commensal Escherichia coli from broiler chickens. A total of 1200 E. coli strains were characterized from 20 broiler farms at three-time points along the production period. All strains were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured by repetitive extragenic palindromic (REP)-PCR fingerprinting. Genomic diversity and characterization were performed by whole genome sequencing (WGS). Twenty-five percent of the commensal E. coli strains harbored mcr-1 genes. Frequency of mcr-1 gene detection correlated positively (odds ratio 1.71; 95% CI 0.96–3.06; p = 0.068) with the use of colistin in poultry flocks. REP-PCR profiles and WGS analysis showed diverse E. coli population carrying multiple antimicrobial resistance genes. Phylogenetic comparison of mcr-1-bearing strains recovered from this study with a global strain collection revealed wide phylogenetic relationship. This study identified a high prevalence of mcr-1 gene among genetically diverse E. coli populations from broiler chickens in Bangladesh suggesting a massive horizontal spread of mcr-1 rather than by clonal expansion.

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