scholarly journals Fungal Contamination of Some Medicinal Plant Materials Sold at Omdurman Local Market, Khartoum State, Central Sudan

2021 ◽  
pp. 85-92
Author(s):  
Ehsan Musa Ewad EL-Kareem ◽  
Hatil Hashim EL-Kamali ◽  
Ahlam Salih Eltahir ◽  
Hanan Ibrahim Mudawi
Keyword(s):  
Medicinal Plant ◽  
Nigella Sativa ◽  
Herbal Medicines ◽  
Cyperus Rotundus ◽  
Lepidium Sativum ◽  
Local Market ◽  
Fungal Contamination ◽  
Foeniculum Vulgare ◽  
Plant Materials ◽  
Cymbopogon Schoenanthus

Plants have long been used as herbal medicines in many countries. However, microbial contamination of these medicines may affect human health. In the present study fifteen medicinal plants namely, Acacia nilotica ssp. nilotica, Trigonella foenum-greacum, Nigella sativa, Hyphaene thebaica, Nauclea latifola, Cyperus rotundus, Cymbopogon schoenanthus spp, proximus, Artemisia herba-alb, Cassia acutifolia, Solenostemma argel, Tamarindus indica, Ziziphus spina-christi, Lepidium sativum, Foeniculum vulgare and Coriandrum sativum were evaluated for their fungal contamination. The pour plate method was used to cultivate serially diluted portions of the medicinal plant material investigated. The colonies of fungi were identified morphologically according to form and pigment. They were observed microscopically through Lactophenol cotton bluestaining. The identified fungal isolates were consist of three fungal species, the most dominant fungi were Aspergillus nigar and Aspergillus flavus. Penicillium ssp is least one. The total fungal  counts ranged from 00x101  cfu/g (Cymbopogon schoenanthus spp.proximus, Trigonella foenum-greacum, Artemisia herba alb, Lepidium satinum , Nigella sativa Cassia acutifolia  and Naucle latifola) to 11x105cfu/g (Cyperus rotundus)..  The highest total fungal load was found in Cyperus rotundus The levels of contamination varied greatly between the commercially available plant samples investigated.

scholarly journals Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn

2020 ◽  
Vol 9 (1) ◽  
pp. 40-45
Author(s):  
O. V. Trineeva ◽  
A. A. Gudkova ◽  
M. A. Rudaya
Keyword(s):  
Medicinal Plant ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Plant Materials ◽  
Fatty Oil ◽  
Luminescent Analysis ◽  
Diagnostic Signs ◽  
Promising Source

Introduction. Sea buckthorn (Hippophae rhamnoides), the family Eleagnaceae – a promising source of biologically active substances, the traditional raw materials of which are fruits. The existing regulatory documentation (ND) lacks such an indicator of the authenticity and good quality of the fruit as «microscopy». Despite the available data on the anatomy and histology of the fruits of this type of medicinal plant material (MPM), the scientific literature does not describe the luminescence features of the tissues of sea buckthorn fruit. It is known that the luminescent features of tissues make it possible to identify the localization of biologically active structures, and also, in some cases, to conduct selective diagnosis of MPM.Aim. The aim of this work was to study the characteristics of the luminescence of the tissues of dried crushed fruits of sea buckthorn, and also to optimize the conditions for analysis.Materials and methods. The object of the study was the dried, crushed fruits of sea buckthorn of various species. The study of microdiagnostic signs was carried out according to the State Pharmacopoeia of the XIV RF GPA.1.5.3.0003.15 «Technique of microscopic and microchemical studies of medicinal plant materials and herbal medicines». A stereomicroscopic study was carried out on a Biomed-6 microscope. The luminescence of sea buckthorn fruit tissues was examined using a luminescent microscope of the Micromed-3 Lum brand.Results and discussion. The most pronounced yellow glow is observed for the tissue of the pulp and epidermis of the fetus, which is associated with the highest content of fatty oil in these structures. Groups of phenolic compounds contained along with oil give a greenish tint to objects, while condensed tannins give a brownish tint. The plates that make up the corymbose hairs do not exhibit bright luminescence; a weak glow is characteristic of the joints of the hair platelets. The walls of stellate hairs have a faint greenish glow. Fragments of the actual fruit («sac») are brownish in color, without their own luminescence, as well as the seed peel. The embryo has its own greenish luminescence due to the presence of both a fatty oil and a complex of storage substances.Conclusion. The luminescent analysis of sea buckthorn fruits was carried out for the first time. The choice of a method for preparing the studied MPM for microscopic examination is experimentally substantiated. The luminescent analysis revealed the peculiarities of the glow of the tissues of the fruits of sea buckthorn. The main microdiagnostic signs of crushed dried sea buckthorn fruits and their biometric characteristics are specified. The analysis of the obtained data will allow us to further develop the section «Microscopic signs» for inclusion in the PA project on MPM, widely cultivated and used by the domestic pharmaceutical industry for the production of herbal medicines.

scholarly journals In Vitro Antibacterial Activities of Various Ethanolic Medicinal Plant Extracts Against Some Human Pathogenic Bacteria

2020 ◽  
Vol 8 (6) ◽  
pp. 1272-1276
Author(s):  
Samir K. Ali ◽  
Ghorbat S. Ali ◽  
Berivan Abdulrahman Abdullah
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Nigella Sativa ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Foeniculum Vulgare ◽  
Medicinal Plant Extracts

The widespread use of antibiotics often causes increase in the bacterial drugs resistance and causes many side effects in humans. Medical plants have antimicrobial effects against most pathogenic bacteria and can serve as harmless replacement to antibiotics. The aim of this study was to assess the antibacterial effect of five medicinal plant prevailed in Kurdistan region namely; Cinnamon (Cinnamomum cassia (L.) D.Don), Nigella (Nigella sativa L.), Allium (Allium hirtifolium Boiss.), Carrot (Daucus carota L.), and Fennel (Foeniculum vulgare Mill.) against pathogenic Gram positive bacteria (Staphylococcus aureus), and pathogenic Gram negative bacteria (Salmonella typhi and Escherichia coli). The evaluation of antibacterial activity for these plant extracts was carried out using agar-well diffusion method. Results showed that minimum inhibitory concentration (MIC) of A. hirtifolium against tested bacterial isolates were (25 mg/mL), the lowest MIC values for S. aureus were (25 mg/mL) observed with C. cassia, N. sativa and F. vulgare ethanolic extracts and the lowest MIC of D. carota against bacterial isolates were (50 mg/mL), Also, it was observed that S. aureus was more sensitive than S. typhi and E. coli to plant extracts. The ethanol plant extracts had potential antibacterial activities. However, further studies are required to identify the active compounds which could be used for the preparation of new antimicrobial agents and control the bacterial infectious diseases.

Nigella Sativa: An immunity booster as pandemic treatment solution against SARS-CoV-2

Coronaviruses ◽  
2020 ◽  
Vol 01 ◽  
Author(s):  
Afzal Hussain ◽  
Ashfaq Hussain ◽  
Chandan Kumar Verma
Keyword(s):  
Medicinal Plant ◽  
Therapeutic Efficacy ◽  
Nigella Sativa ◽  
Folk Medicine ◽  
The World ◽  
Immune Booster

: Nigella sativa (Family Ranunculaceae) is a common medicinal plant all across the world. It is quite popular in different traditional medicinal systems such as Unani, Ayurveda, Tibb, and Siddha. Oil and Seeds have a long tradition of folk medicine utilized in different medicinal systems and food. The seeds of N. Sativa have indeed been widely applied in the treatment of many diseases, ailments, and also the immune booster. Our goal primarily concentrated on the therapeutic efficacy of Nigella sativa in combating the COVID-19 pandemic.

scholarly journals Biological activity from indigenous medicinal plants of Mauritius

2005 ◽  
Vol 77 (1) ◽  
pp. 41-51 ◽  
Author(s):  
A. Gurib-Fakim ◽  
H. Subratty ◽  
F. Narod ◽  
J. Govinden-Soulange ◽  
F. Mahomoodally
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Biological Properties ◽  
Pharmacological Properties ◽  
Plant Materials ◽  
In Vivo Screening ◽  
Medicinal Plant Extracts ◽  
Minor Ailments

The Mauritian population has a long tradition in the use of ethno-medicine, and the practice is still strong, especially in the treatment of minor ailments. Such interest stems from an existing culture, and many “tisanes” are still prepared from plant materials and sold in several markets around the island.This paper will focus on the various chemical/biological screening techniques currently being used to evaluate the biological properties of medicinal plant extracts. Particular emphasis will be put on extraction and various screening for biological/pharmacological properties. Due consideration will be given to the pharmacological approaches that utilize different animal models for the in vitro and in vivo screening of medicinal plant extracts.

Antiviral and Immunity-modulating Natural Herbs in the Prevention of COVID-19

2021 ◽  
pp. 81-94
Author(s):  
Sonali S Gadge
Keyword(s):  
Immune System ◽  
Medicinal Plants ◽  
Nigella Sativa ◽  
Curcuma Longa ◽  
Antiviral Drug ◽  
Herbal Medicines ◽  
Echinacea Purpurea ◽  
Black Pepper ◽  
Tinospora Cordifolia ◽  
Ocimum Sanctum

The COVID-19 has spread all over the world and there is no single drug or medicine for the prevention and the treatment of this deadly disease. One can get prevention from this disease by boosting the immune system. Persons with weak immune system e.g. children, elder patients or patients with preexisting diseases affect rapidly due to coronavirus. This novel coronavirus attacks mainly on the person immunity. For the centuries the herbal medicinal plants have many beneficial effects against deadly viruses. Herbal medicines provide a rich resource for new antiviral drug development. These natural agents interact with the life cycle of virus such as virus entry, replication, assembly and release. In this review, the importance, immunomodulator, antiviral and pharmacological activities of some medicinal plants, namely, Allium Sativum, Zingiber Officinalis, Cinnamomum zeylanicum, Curcuma longa, Ocimum sanctum, Glycyrrhiza glabra, Ginseng, Black pepper, Emblica officinalis, Withania somnifera, Tinospora cordifolia, Echinacea purpurea, Azadirachta indica, Piper betel, Eugenia caryophyllata, Cinchona calisaya, Nigella sativa, Cuminum cyminum are highlighted. These medicinal plants have many phytoconstituents like alkaloids, glycosides, terpenoids, flavonoids, lactones, polysaccharides, volatile oils, tannins, resins, etc which are helpful for boosting immunity and will help in the prevention and in the management of COVID-19. Several types of research attempted to use herbal medicines against COVID-19.

scholarly journals Quality Control of Herbal Medicines: From Traditional Techniques to State-of-the-art Approaches

Planta Medica ◽  
2021 ◽  
Author(s):  
Luiz C. Klein-Junior ◽  
Maira R. de Souza ◽  
Johan Viaene ◽  
Tania M. B. Bresolin ◽  
André L. de Gasper ◽  
...  
Keyword(s):  
Quality Control ◽  
Data Analysis ◽  
Plant Extracts ◽  
Herbal Medicines ◽  
Botanical Origin ◽  
Plant Materials ◽  
Holistic View ◽  
Marker Selection ◽  
Chromatographic Profile ◽  
Oriented Approach

AbstractHerbal medicines are important options for the treatment of several illnesses. Although their therapeutic applicability has been demonstrated throughout history, several concerns about their safety and efficacy are raised regularly. Quality control of articles of botanical origin, including plant materials, plant extracts, and herbal medicines, remains a challenge. Traditionally, qualitative (e.g., identification and chromatographic profile) and quantitative (e.g., content analyses) markers are applied for this purpose. The compound-oriented approach may stand alone in some cases (e.g., atropine in Atropa belladonna). However, for most plant materials, plant extracts, and herbal medicines, it is not possible to assure quality based only on the content or presence/absence of one (sometimes randomly selected) compound. In this sense, pattern-oriented approaches have been extensively studied, introducing the use of multivariate data analysis on chromatographic/spectroscopic fingerprints. The use of genetic methods for plant material/plant extract authentication has also been proposed. In this study, traditional approaches are reviewed, although the focus is on the applicability of fingerprints for quality control, highlighting the most used approaches, as well as demonstrating their usefulness. The literature review shows that a pattern-oriented approach may be successfully applied to the quality assessment of articles of botanical origin, while also providing directions for a compound-oriented approach and a rational marker selection. These observations indicate that it may be worth considering to include fingerprints and their data analysis in the regulatory framework for herbal medicines concerning quality control since this is the foundation of the holistic view that these complex products demand.

scholarly journals A Review of the Toxicity and Phytochemistry of Medicinal Plant Species Used by Herbalists in Treating People Living With HIV/AIDS in Uganda

2021 ◽  
Vol 12 ◽  
Author(s):  
G. Anywar ◽  
E. Kakudidi ◽  
R. Byamukama ◽  
J. Mukonzo ◽  
A. Schubert ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Plant Species ◽  
Plant Extracts ◽  
Herbal Medicines ◽  
Medicinal Plant Species ◽  
People Living With Hiv ◽  
Oral Toxicity ◽  
Cytotoxicity Studies ◽  
Living With Hiv ◽  
Hiv Aids

Introduction: Despite concerns about toxicity, potentially harmful effects and herb-drug interactions, the use of herbal medicines remains widely practiced by people living with HIV/AIDS (PLHIV) in Uganda.Objective: The objective of the paper was to comprehensively review the literature on the toxicity and chemical composition of commonly used medicinal plant species in treating PLHIV in Uganda.Methods: We reviewed relevant articles and books published over the last sixty years on ethnobotany, antiviral/anti-HIV activity, toxicity, phytochemistry of Vachellia hockii, Albizia coriaria, Bridelia micrantha, Cryptolepis sanguinolenta, Erythrina abyssinica, Gardenia ternifolia, Gymnosporia senegalensis, Psorospermum febrifugium, Securidaca longipendunculata, Warburgia ugandensis and Zanthoxylum chalybeum and their synonyms. We searched PubMed, Web of Science, Scopus, Science Direct and Google Scholar.Discussion: Most of the plant species reviewed apart from P. febrifugium, S. longipedunculata and C. sanguinolenta lacked detailed phytochemical analyses as well as the quantification and characterization of their constituents. Crude plant extracts were the most commonly used. However, purified/single component extracts from different plant parts were also used in some studies. The U87 human glioblastoma was the most commonly used cell line. Water, ethanol, methanol and DMSO were the commonest solvents used. In some instances, isolated purified compounds/extracts such as Cryptolepine and Psorospermin were used.Conclusion: Cytotoxicity varied with cell type, solvent and extract type used making it difficult for direct comparison of the plant species. Five of the eleven plant species namely, A. coriaria, C. sanguinolenta, G. ternifolia, P. febrifugium and Z. chalybeum had no cytotoxicity studies in animal models. For the remaining six plant species, the crude aqueous and ethanol extracts were mainly used in acute oral toxicity studies in mice. Herbalists reported only A. coriaria and W. ugandensis to cause toxic side effects in humans. However, selective cytotoxic plant extracts can potentially be beneficial as anticancer or anti-tumour drugs.

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