scholarly journals Larval susceptibility of Aedes Aegypti (L.) (Diptera: Culicidae) to extracts of Ilex Paraguariensis and Ilex Theezans

2016 ◽  
pp. 113-120 ◽  
Author(s):  
Ana Carla Knakiewicz ◽  
◽  
Junir Antonio Lutinski ◽  
Carin Guarda ◽  
Ariane Paris ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Ilex Paraguariensis ◽  
Larval Susceptibility

scholarly journals Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

2018 ◽  
Vol 19 (1) ◽  
pp. 31-36 ◽  
Author(s):  
HASMIWATI HASMIWATI ◽  
SELFI RENITA RUSJDI ◽  
EKA NOFITA
Keyword(s):  
Aedes Aegypti ◽  
Novel Mutation ◽  
Hemorrhagic Fever ◽  
Gene Encoding ◽  
Development Of Resistance ◽  
West Sumatra ◽  
Primary Vector ◽  
Endemic Areas ◽  
Larval Susceptibility

Hasmiwati, Rusjdi SR, Nofita E. 2018. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia. Biodiversitas 19: 31-36. Aedes aegypti is distributed widely in West Sumatra as a primary vector of Dengue hemorrhagic fever, especially in Padang City. Synthetic insecticide control is one currently used method to prevent mosquito-borne diseases. The extensive, long-term application of Temephos along with inappropriate dosages, have resulted in the development of resistance in Ae. aegypti populations. Mutation of the Ace-1 gene, encoding an acetyl cholinesterase, is one of the mechanisms that confer resistance to organophosphate (OP). The Temephos resistance status of Ae. aegypti in Padang city has not yet been studied. This study aimed to investigate the resistance status of Ae. aegypti and identify any possible mutation (s) of the Ace-1 gene in Padang city. Ae. aegypti samples were collected in five population in Padang city (Jati (JT), Gunung Pangilun (GP), Lubuk Minturun (LM), Korong Gadang (KG), and Bandar Buat (BB)). The larval susceptibility to Temephos was tested by larval bioassays with Temephos pestanal at 0.02 mg/L dosages. Larval susceptibility was determined by mortality percentage values. The relationship between Ace-1 genotypes and the resistant phenotype was analyzed by percentage of genotype frequency. Out of five populations, assessed by larval bioassays, JT and GP were resistant to Temephos; LM, KG, and BB were tolerant. A total of 50 individuals from larval bioassays were genotyped for Ace-1 gene. Our findings showed that Ace-1 was 495 bp in length. Mutation was not found in the G119S location but in the T506T location. Three alleles in T506T location were detected, including a wild type allele, TT (65.21%), and two mutant alleles, TA (26.08%), AA (8.69%). The use of Temephos showed that some Ae. aegypti populations were resistant, others were tolerant, but no population was vulnerable to Temephos. A novel mutation was detected as substitution in T506T location (ACT>ACA).

scholarly journals How long is long enough? Decreasing effects in Aedes aegypti larval mortality by plant extracts over time

2021 ◽  
Vol 56 (2) ◽  
pp. 338-345
Author(s):  
Gilberto Dinis Cozzer ◽  
Renan de Souza Rezende ◽  
Junir Antônio Lutinski ◽  
Walter Antônio Roman ◽  
Maria Assunta Busato ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Plant Extracts ◽  
Mosquito Control ◽  
Population Control ◽  
Larval Mortality ◽  
Residual Effect ◽  
Ilex Paraguariensis ◽  
Short Time Scale ◽  
Human Intoxication ◽  
Short Time

Aedes aegypti has overcome all kinds of mosquito control attempts over the last century. Strategies for population control resorts to the use of synthetic insecticides, which can lead to problems like human intoxication and environmental contamination. The effects of Bacillus thuringiensis var. israelensis (Bti), Ilex paraguariensis (yerba mate), and Ilex theezans (caúna herb) extracts against A. aegypti larvae were evaluated. The bioassays were conducted under controlled laboratory conditions of temperature (27 ± 3°C) and photoperiod (12 h). Hydroalcoholic extract of the leaves of I. theezans displayed better residual effect compared to the aqueous extract of I. paraguariensis fruits. The strongest residual effect of I. theezans was probably due to the presence of certain chemicals in its leaves, such as coumarins, hemolytic saponins, and cyanogenic glucosides, which were absent in I. paraguariensis. The results herein contributed to the prospection of natural insecticides and opened the possibility for subsequent studies on the use of plant extracts in field situations in a short-time scale.

scholarly journals How long is long enough? Decreasing on plant extract lethal effects, over time, affecting Aedes aegypti larval mortality

2019 ◽  
Author(s):  
Gilberto Dinis Cozzer ◽  
Renan de Souza Rezende ◽  
Junir Antônio Lutinski ◽  
Walter Antônio Roman Junior ◽  
Maria Assunta Busato ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Population Control ◽  
Larval Mortality ◽  
Food Resource ◽  
Residual Effect ◽  
Alternative Methods ◽  
Ilex Paraguariensis ◽  
Short Time Scale ◽  
Over Time

AbstractThe mosquito Aedes aegypti has overcome all kinds of human being mosquito control attempts over the last century. Strategies for vector population control resorts to the use of synthetic insecticides, which can lead to problems of intoxication in humans and environmental contamination. We evaluated the effects of Bacillus thuringiensis var. israelensis (Bti), Ilex paraguariensis (mate-herb) and Ilex theezans (caúna-herb) extracts against A. aegypti larvae mortality. The bioassays were conducted under controlled laboratory conditions of temperature (27±3°C) and photoperiod (12h). Hydroalcoholic I. theezans leaves extract displayed better residual effect compared to I. paraguariensis fruit aqueous extract. Variation in larval mortality was also observed in the exposure periods (low after a few weeks). Low mortality after a few weeks may mean increased the food for mosquito in a oppose effect over time. The residual effect of Bti was observed during the 56 days of the study duration (100% of mortality). The strongest residual effect of I. theezans was probably due to the presence of chemical on its leaves, such coumarins, hemolytic saponins and cyanogenic glucosides, absent in I. paraguariensis. On the other hand, alternative methods to vector control present risks in a long term scale by reversal of larvicide effect into food resource. Our results contributed to the prospection of natural insecticides and open the possibility for subsequent studies of the use of plant extracts in field situations in a short time scale.

scholarly journals Larvicidal activity of aqueous extracts of Ilex paraguariensis and Ilex theezans on Aedes aegypti (L.)

2020 ◽  
Vol 42 ◽  
pp. e3
Author(s):  
Ana Carla Knakiewicz ◽  
Junir Antonio Lutinski ◽  
Maria Assunta Busato ◽  
Walter Antônio Roman Junior ◽  
Daniel Albeny Simões
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Zika Virus ◽  
Ilex Paraguariensis ◽  
Mosquito Larvae ◽  
Yerba Mate ◽  
Aqueous Extracts ◽  
Plant Variation ◽  
Chikungunya Fever ◽  
Controlled Conditions

Aedes aegypti is the main vector of Dengue, Yellow Urban Fever, Chikungunya fever and Zika virus fever. The strategies for its control include synthetic products that cause damage to the environment and other organisms. This study evaluated the larvicidal activity of aqueous extracts of leaves and fruits of Ilex paraguariensis and Ilex theezans on Aedes aegypti mosquito larvae. The bioassays were conducted at the Ecological Entomology Laboratory, Unochapecó, under controlled conditions of temperature and photoperiod. The extracts of fresh and dried leaves of I. theezans showed greater larvicidal activity when compared to extracts of fruits of the same plant. Variation in larvicidal activity was also observed during exposure periods. The results suggest the use of extracts of these plants in the control of A. aegypti and the prospection of substances that can be used as an alternative to synthetic products. They point to the possibility of using yerba mate tissues that are not used commercially.

scholarly journals POTENCIAL LARVICIDA DE MELIA AZEDARACH L. E ILEX PARAGUARIENSIS ST. HIL. NO CONTROLE DE AEDES AEGYPTI (LINNAEUS, 1762) (DIPTERA: CULICIDAE)

2015 ◽  
Vol 37 (2) ◽  
Author(s):  
Maria Assunta Busato ◽  
Jaqueline Vitorello ◽  
Junir Antonio Lutinski ◽  
Jacir Dal Magro ◽  
Jaqueline Scapinello
Keyword(s):  
Aedes Aegypti ◽  
Melia Azedarach ◽  
Ilex Paraguariensis

GAMBARAN INDEKS LARVA AEDES AEGYPTI DI BUFFER WILAYAH KERJA BANDARA SEPINGGAN BALIKPAPAN

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Abdurrakhman abdurrakhman Abdurrakhman
Keyword(s):  
Aedes Aegypti ◽  
Buffer Zone ◽  
Chi Square ◽  
Cross Sectional ◽  
Breeding Sites ◽  
Dengue Disease ◽  
Vector Borne Diseases ◽  
Transportation Equipment ◽  
Chi Square Test ◽  
Larvae Density

ABSTRACT : The House index and Container Index in the buffer area of ​​the working area of ​​Balikpapan Sepinggan Airport is still above 1%, so the potential for the spread of dengue disease. Mobilization of people, goods and transportation equipment will increasingly affect the transmission of disease in ports and airports, especially for vector-borne diseases. This study aims to analyze the risk factors associated with larvae density of Aedes aegypti and describe the larvae index in the buffer zone of the Sepinggan Balikpapan Airport This study was a descriptive study with a cross sectional design. The sample in this study was 121 houses with a proportionate stratified random sampling, the research location was in the buffer zone of Sepinggan Balikpapan Airport in November 2018. The variables studied were houses with positive larvae containers, breeding sites and PSN behavior and larvasidation. The data was analyzed using the chi square test. There was a relationship between houses with larvae positive Aedes aegypti, behavior of Mosquito Nest Eradication (PSN) and larvasidation with larvae density of Aedes aegypti but not for breeding sites (p = 0.00 and 95% CI = 0.64), and   (p = 0.00 and 95% CI = 0.34). The description of several Aedes aegypti larvae index, namely House Index (HI) = 57.02%, Container Index (CI) = 24.36%, Bruteau Index (BI) = 148.76, and Flick Free Numbers (ABJ) = 42.98 %. Houses with larvae of Aedes aegypti larvae and PSN and larvasidation behavior were associated with larvae density of Aedes aegypti. The index of HI, CI and BI larvae is of high value so there is a risk of DBD transmission

Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran
Keyword(s):  
Early Intervention ◽  
Early Detection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Transmission Season ◽  
Aedes Mosquitoes ◽  
Alternative Approach ◽  
Human Sera ◽  
Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

Distribution of Aedes aegypti and Aedes albopictus in different eco zones of Thiruvananthapuram city with special reference to dengue viremia in humans

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 223-230
Author(s):  
S. Sunil Kumar ◽  
D.A. Evans ◽  
K. Muthulakshmi ◽  
T. DilipKumar ◽  
R. Heera Pillai ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Dengue Shock Syndrome ◽  
Single Step ◽  
Equal Distribution ◽  
Distribution Study ◽  
Urban Zone ◽  
High Prevalence ◽  
Vector Management ◽  
Efficient Vector

Mosquito index study of three ecologically different ecozones of the Thiruvananthapuram district, Kerala showed sharp difference on the proportionate distribution of Aedes aegypti and Aedes albopictus. Human dengue viremia (HDV) was very high in those ecozones where A.aegypti density was high and HDV was low where A.albopictus was high. In a coastal zone of Thiruvananthapuram city, A. aegypti was the most abundant vector and in a hilly, arid suburban zone, A.albopictus was the abundant vector. In the urban zone both species of mosquitoes showed equal distribution. Study on the circulating serotypes in the serum of HDV by Single step single tube Multiplex PCR showed all the four serotypes viz DENV1, DENV2, DENV3 and DENV4 in patients of Thiruvananthapuram city, which indicated the possibility of Dengue Shock Syndrome, unless there is efficient vector management. Among the four dengue serotypes, Type 1 was the most abundant virus. Abundance of microhabitats in Thiruvananthapuram city, which support A. aegypti may be the reason for high prevalence of dengue fever in the urban zone.

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