scholarly journals CONTRIBUIÇÃO AO ESTUDO FITOQUÍMICO E ANÁLITICO DO Nasturtium officinale R. Br., BRASSICACEAE

2002 ◽  
Vol 3 (2) ◽  
Author(s):  
J. L. S. CARVALHO ◽  
O. G. MIGUEL
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Analytical Study ◽  
Analytical Techniques ◽  
Phytochemical Study ◽  
Nasturtium Officinale ◽  
Aerial Parts ◽  
Traditional Process ◽  
Layer Chromatography ◽  
Brassicaceae Family

O presente trabalho, realizou o estudo sobre Nasturtium officinale R. Br.; pertencente a família Brassicaceae, cujas partes aéreas do vegetal foram submetidas a estudo botânico para obtenção das características anatômicas do mesmo. Aliado realizou-se o estudo fitoquímico utilizando como técnica analítica a cromatografia em camada delgada, cromatografia em coluna, cromatografia gasosa, cromatografia líquida de alta eficiência e espectrometria de ultra violeta. Foram desenvolvidas tecnologias extrativas de particionamento por processo tradicional em Soxhlet, nos quais pode-se verificar eficiência e reprodutibilidade para fins farmacotécnicos, farmacológicos e preparativos. De acordo coma literatura consultada, verificou- -se a presença de compostos fenólicos simples e heterosídicos (fenilpropoanóides e flavonóides), saponinas (esteróidais e terpênicas policíclicas) e compostos contendo enxofre (glucosinolatos). De acordo com análise comparativa dos processos extrativos (tradicional e Soxhlet), pode-se referir que o processo Soxhlet apresentou melhor rendimento e rapidez, alémde apresentar-se termoestável para a concentração e isolamento de grupos estruturais, para fins analíticos, farmacotécnicos e farmacológicos. A CONTRIBUTION TO PHYTOCHEMICAL AND ANALYTICAL STUDY OF Nasturtium officinale R. Br., BRASSICACEAE Abstract The present is concerned to a study about Nasturtium officinale R. Br.; Brassicaceae family, which aerial parts were submitted to o botanical study to obtain its anatomic characteristics. It was also conducted the phytochemical study and using as analytical techniques in thin layer chromatography, column chromatography, gaseous chromatography, high performance liquid chromatography and ultraviolet spectrometry. It was developed extractive technologies of partition by traditional process in Soxhlet, through which it was possible to verify efficiency and reproducibility to pharmacothechinical and pharmacological methods, and to preparative objectives too. According to the literature, it was verified the presence of simple and heterosidic phenolic compounds (phenylpropanoids and flavonoids), saponinas (steroidal and policyclic terpenic) and compounds containing sulfur (glucosinolates). According to the comparative analysis of the extractive processes (traditional and Soxhlet), it was possible to say that the Soxhlet process presented a better performance and speed, besides being thermo-stable to the concentration and isolation of the structural groups , to pharcothecnical, pharmacological and analytical objectives.

scholarly journals Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

2020 ◽  
Vol 29 (2) ◽  
pp. 27-36
Author(s):  
Alaa M. Abd ◽  
Enas J. Kadhim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Aqueous Methanol ◽  
Plant Materials ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Soxhlet Apparatus ◽  
Layer Chromatography

 The aim of this study was to study chemical constituents of aerial parts of Cardaria draba since no phytochemical investigation had been studied before in Iraq. Aerial parts of Cardaria draba were defatted by maceration in hexane for 72 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous Methanol 90% as a solvent extraction for 18 h, and fractionated with petroleum ether- chloroform (CHCl3)- ethylacetate- and n-butanol respectivly. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions were investigated by high performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for its phenolic acid and flavonoid contents. Flavonoids and phenolic acid derivative were isolated from the ethylacetate of leaf fraction and n-butanol after hydrolysis fraction of the aerial parts and identified by TLC, FTIR and HPLC. A various chromatographic and spectroscopic results shown the presence of luteolin, chlorogenic acid, caffeic acid, and resorcinol in aerial parts of C. draba.                                                                                                                              

scholarly journals ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF COMBINATION OF ANTI-ASTHMATIC DRUGS MONTELUKAST AND DOXOFYLLINE

2021 ◽  
Vol 11 (6) ◽  
pp. 86-91
Author(s):  
Sachin N. Kapase
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Method Development ◽  
Analytical Techniques ◽  
Ultra Performance Liquid Chromatography ◽  
Hplc Method ◽  
Uv Spectrophotometry ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Layer Chromatography

For qualitative and quantitative analysis, various analytical techniques are available such as Ultraviolet (UV) Spectrophotometry, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC). As per literature survey, there are some UV, HPLC, Ultra-Performance Liquid Chromatography (UPLC) and HPTLC analytical methods are developed for Montelukast and Doxofylline individually and in a combination with other drugs too, since yet there are no significant stability studies indicating HPLC method reported for Montelukast and Doxofylline combinations. In the current study, the HPLC method is developed and validated for simultaneous quantitative estimations of Montelukast and Doxofylline. These present techniques are more efficient and sensitive as compared to other analytical techniques.

scholarly journals Identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea

2019 ◽  
Vol 18 (2) ◽  
pp. 73-77
Author(s):  
L. M. Fedoseeva ◽  
Yu. I. Chistova
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Uv Light ◽  
Spectral Characteristics ◽  
Water System ◽  
Dry Extract ◽  
Caffeic Acid Derivatives ◽  
Layer Chromatography

The purpose of this work is to study of phenolic compounds in the dry extract of dandelion herb and large burdock leaf tea.Materials and methods . The separation and identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea by thin-layer chromatography and high-performance liquid chromatography with UV-detectionhas been carried out.Results . As a result of research, it has been established that during TLC the optimal system for the separation of phenolic compounds is the ethyl acetate – formic acid – water system (10:2:3). On the chromatogram four spots were found corresponding to the value of Rf and fluorescence in UV-light to flavonoids of the flavone group and phenolic acids (chlorogenic and caffeic acids). For further identification of phenolic compounds using HPLC, eight peaks were found, which in terms of retention time and spectral characteristics correspond to phenologlycosides, chlorogenic acid, caffeic acid derivatives, ferulic acid, umbelliferone.Conclusions . Thus, the dry extract of dandelion herb and large burdock leaf tea contains hydroxycinnamic acids and their derivatives, compounds of coumarin nature, phenologlycosides.

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