scholarly journals Asymptomatic Bacteriuria and Antibacterial Susceptibility Patterns in an Obstetric Population

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Şevki Çelen ◽  
Ayla Sargin Oruç ◽  
Rana Karayalçin ◽  
Sibel Saygan ◽  
Serpil Ünlü ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Urine Analysis ◽  
Asymptomatic Bacteriuria ◽  
High Sensitivity ◽  
Klebsiella Pneumonia ◽  
Low Birth Weight Infants ◽  
E Coli ◽  
Antibacterial Susceptibility ◽  
Obstetric Population ◽  
Associated Risk Factors

Introduction. Asymptomatic bacteriuria (ASB), occurring in 2–11% of pregnancies, is a major predisposition to the development of pyelonephritis, which is associated with obstetrical complications, such as preterm labor and low birth weight infants. The aim of this study was to determine the prevalence of ASB, the antibacterial susceptibilities of the isolated microorganisms and the associated risk factors in an outpatient clinical setting in Zekai Tahir Burak Women's Health Education and Research Hospital in Ankara, Turkey. Material and Methods. Between December 2009 and May 2010, pregnant women admitted to the antenatal outpatient clinic were included in this study. The results of a complete urine analysis, midstream urine culture and antibacterial susceptibility were evaluated. Results. Of the 2011 pregnant women included, 171 had ASB (8.5%). E. coli was the most frequently isolated microorganism (76.6%), followed by Klebsiella pneumonia (14.6%). Both microorganisms were highly sensitive to fosfomycin, sensivity being 99.2% for E. coli and 88% for Klebsiella pneumonia. Conclusions. In this certain geographical region, we found E. coli as the most common causative agent of ASB in the obstetric population and it is very sensitive to fosfomycin. We recommend fosfomycin for ASB in pregnant women due to its high sensitivity, ease of administration and safety for use in pregnancy.

scholarly journals Asymptomatic Bacteriuria Among Pregnant Women in Debre Markos, Ethiopia: Prevalence, Risk Factors, and Antimicrobial Susceptibility of Isolates

2021 ◽  
Author(s):  
Yihunalem Addis ◽  
Sirak Biset ◽  
Mucheye Gizachew
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Low Income ◽  
Asymptomatic Bacteriuria ◽  
Antibiotics Resistance ◽  
P Value ◽  
E Coli ◽  
Associated Risk Factors ◽  
Susceptibility Profiles

Abstract In low-income countries, asymptomatic bacteriuria (ASB) during pregnancy is a major cause for both maternal and foetal health risks. Rapid emergence of antimicrobial resistance also needs continuous monitoring of susceptibility profiles of uropathogens. We conducted a hospital-based cross-sectional study to deterimne prevalence, antimicrobial susceptibility, and associated risk factors of ASB among pregnant women. Socio-demographic and clinical data were collected by interview and extracted from women's medical records. Identification of bacteria from urine, and their susceptibility tests were done by using recommended methods. Logistic regression analyses were done by SPSS versions 20. The p-value <0.05 at 95% CI was considered as statistically significant. Of the 172 study participants, 24 (14%) had ASB. Among 24 isolates, 13 (54.2%) were gram-negative, and of these, E. coli (8; 61.5%) was predominant followed by K. pneumoniae (4; 30.8%). Previous UTI and antibiotic use were significantly associated risk factors for ASB. E .coli, S. areus and CoNS were resistant to tetracycline (87.5%), cotrimoxazole (83.3%), and gentamycin (80%), respectively. Prevalence of ASB was lower than many previous reports in the country. Commonly used antibiotic susceptibility profiles of isolates were known. Hence, hard work ought to assume to decline the consequence of ASB and antibiotics resistance.

scholarly journals Asymptomatic Bacteriuria among Pregnant Women Attending Tertiary Care Hospital in Lucknow, India

2021 ◽  
pp. 1-8
Author(s):  
Naimshree Sonkar ◽  
Malay Banerjee ◽  
Suman Gupta ◽  
Absar Ahmad
Keyword(s):  
Haemoglobin Level ◽  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Asymptomatic Bacteriuria ◽  
Care Hospital ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli

Introduction: Asymptomatic bacteriuria (ASB) is the presence of actively multiplying bacteria within the urinary tract with absence of any symptoms, resulting in adverse pregnancy outcomes. This research study was done in order to review prevalence, antimicrobial susceptibility profile, and factors associated with ASB occurring in female patients who are pregnant and being treated at a tertiary care hospital in Lucknow, India. Method and Materials: This is a cross-sectional study done among 216 pregnant women attending a hospital for antenatal check-ups. Clean catch midstream urine samples were collected and examined microscopically, and semi-quantitative culture was done on blood agar and MacConkey agar. Isolates were identified by colony morphology and biochemical tests, and antimicrobial susceptibility testing was done by using the Kirby-Bauer method. Results: Of the 216 pregnant women, 36 (16.7%) tested positive for ASB. The female gestational period, haemoglobin level, and BMI were significantly associated with ASB. Logistic regression also showed that higher haemoglobin level was less likely to ASB (AOR = 0.42, 95% confidence interval: 0.202–0.88, p = 0.021). The predominant and usual isolates were E. coli (n = 22, 61.1%), followed by Cons (n = 6, 16.7%), and S. aureus (3, 8.3%). All Gram-negative isolates were mostly sensitive to most of the drugs like piperacillin-tazobactam, cefepime, nitrofurantoin, and meropenem but were 100% resistant to ampicillin. Similarly, Gram-positive isolates were sensitive to ampicillin, vancomycin, linezolid, and nitrofurantoin but 100% resistant to co-trimoxazole. Conclusion: The present study shows the existence of ASB was 16.7% among women who are pregnant. Pregnancy duration, haemoglobin level, and BMI were significantly associated with ASB. The isolates identified more frequently were E. coli (61.16%), Cons (16.7%), and S. aureus (8.3%). All isolates which were Gram-negative were mostly sensitive to most of the drugs but were 100% resistant to ampicillin. Similarly, Gram-positive isolates were sensitive to most of the drugs but 100% resistant to co-trimoxazole.

scholarly journals The Rate of Asymptomatic Bacteriuria is Similar in Term and Preterm Delivery

2016 ◽  
Author(s):  
Riska Amelia
Keyword(s):  
Pregnant Women ◽  
Preterm Delivery ◽  
Preterm Labor ◽  
Obstet Gynecol ◽  
Urine Analysis ◽  
Urine Culture ◽  
Asymptomatic Bacteriuria ◽  
Significant Difference ◽  
Term Delivery ◽  
The Subject

Objectives: To show the prevalence of asymptomatic bacteriuria in pregnant women and to find its correlation with preterm labor. Methods: This research was performed in Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia. A total of 78 subjects joined this research. The first group consisted of 39 subjects that were having preterm delivery and the second group consisted of 39 subjects that were having term delivery. Urine analysis and culture were done in both groups. The subject was being considered to have asymptomatic bacteriuria if the urine culture showed a minimum of 100,000 bacteria/ml without any symptoms or signs of urinary tract infection. The number was then analyzed to reveal the correlation of asymptomatic bacteriuria with preterm delivery. Results: There were a significantly higher number of preterm deliveries in subjects whose age ranged between 15-19 year-old (28.20% vs 12.8%; p=0.033). While on the contrary, there was a significantly lower number of preterm delivery in subjects whose age ranged between 30-34 year-old (25.6% vs 5.12%; p=0.020).The incidence number of asymptomatic bacteriuria among all subjects is 21.79%. There was no significant difference of asymptomatic bacteriuria between preterm and a term delivery (23.07% vs 20.51%; p=0.784). Conclusions: There was no significant difference of asymptomatic bacteriuria between the subject with preterm delivery and term delivery, but the incidence of asymptomatic bacteriuria in pregnant women in this study was quite high, reaching eight times higher than those stated in other study. Thus, routine urine culture screening policy in early pregnancy still cannot be omitted, considering that asymptomatic bacteriuria can lead to pyelonephritis which can cause maternal and fetal morbidity. [Indones J Obstet Gynecol; 37-1: 8-11] Keywords: asymptomatic bacteriuria, urine analysis, urine culture, preterm delivery

scholarly journals Phylogenetic background of E. coli isolated from asymptomatic pregnant women from Kolkata, India

2015 ◽  
Vol 9 (07) ◽  
pp. 720-724 ◽  
Author(s):  
Mandira Mukherjee ◽  
Snehashis Koley ◽  
Sandip Kumar Mukherjee ◽  
Shreya Basu ◽  
Biplab Ghosh ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pregnant Women ◽  
Statistical Significance ◽  
Asymptomatic Bacteriuria ◽  
Antibiotic Sensitivity ◽  
Etiologic Agent ◽  
Esbl Production ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Resistance Patterns

Introduction: Asymptomatic bacteriuria (ABU) in pregnancy generates medical complications. E. coli is the common etiologic agent responsible for ABU-associated infections. This study aimed to identify the phylogenetic background and drug resistance in asymptomatic E. coli from a pregnant population. Methodology: E. coli was confirmed biochemically from culture-positive urine samples collected from asymptomatic pregnant women. Phylogenetic typing was done by polymerase chain reaction (PCR). The isolates were subjected to antibiotic susceptibility testing and extended-spectrum beta-lactamase (ESBL) production. Statistical significance was determined using SPSS 17.0 software. Results: Bacteriuria was observed in 113 (22.6%) of 500 asymptomatic pregnant females. E. coli was reported in 44 (38.9%) of 113 isolates. The mean age-wise distribution was 25.14 ± 4.63. Although pathogenic phylogroup B2 was predominant (54.5%), incidence of non-pathogenic phylogroup B1 (27.3%) was found to be statistically significant (p ≤ 0.001), and B1 and B2 were correlated with respect to total ABU population. Antibiotic sensitivity against ampicillin (34.1%), ceftazidime (50%), cefotaxime (47.7%), ciprofloxacin (47.7%), amikacin (86.4%), nitrofurantion (79.5%), and co-trimoxazole (36.4%) was observed. Multidrug resistance (MDR) and ESBL production was reported in 26 (59.1%) of 44 and 18 (69.2%) of the 26 MDR isolates, respectively. A significant distribution of phylogroup B1 (p = 0.03) with drug resistance was also observed. Conclusions: This is the first study that reported significant incidence of non-pathogenic phylogroup B1 in asymptomatic E. coli with high incidence of MDR isolated from pregnant women in Kolkata, India.  These varied resistance patterns present major therapeutic and infection control challenges during pregnancy.

scholarly journals Escherichia Coli From Urine Samples of Pregnant Women as an Indicator for Antimicrobial Resistance in the Community: a Field Study From Rural Burkina Faso.

2021 ◽  
Author(s):  
A.S. Post ◽  
I. Guiraud ◽  
M. Peeters ◽  
P. Lompo ◽  
S. Ombelet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Burkina Faso ◽  
Pregnant Women ◽  
Urine Analysis ◽  
Urine Samples ◽  
Clinical Isolates ◽  
Antibiotic Residues ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli

Abstract Introduction: In low- and middle-income countries, surveillance of antimicrobial resistance (AMR) is mostly hospital-based and, in view of poor access to clinical microbiology, biased to more resistant pathogens. We assessed AMR among Escherichia coli isolates obtained from urine cultures of pregnant women as an indicator for community AMR and compared the AMR results with those from E. coli isolates obtained from febrile patients in previously published clinical surveillance studies conducted within the same population in Nanoro, rural Burkina Faso.Results: Between October 2016 – September 2018, midstream urine samples collected as part of routine antenatal in Nanoro district were cultured by a dipslide method and screened for antibiotic residues. Among 6018 consenting women (median (IQR) age 25 (20 - 30)), 84 (1.4%) were excluded because of symptoms of urinary tract infection and 96 (1.6%) screened positive for antibiotic residues. Significant growth - defined as a monoculture of Enterobacterales at counts of ≥ 104 colony forming units/ml – was observed in 202 (3.4%) cultures; E. coli represented 155 (76.7%) of isolates. Among these E. coli isolates, resistance rates to ampicillin, cotrimoxazole and ciprofloxacin were respectively 65.8%, 64.4% 16.2%, compared to 89.5%, 89.5% and 62.5% among E. coli from historical clinical isolates (n = 48 of which 45 from blood cultures). Proportions of extended spectrum beta-lactamase producers and multidrug resistance were 3.2% and 5.2% among E. coli isolates from urine in pregnant women versus 35.4%, and 60.4% respectively among clinical isolates. Adding urine culture to the routine urine analysis (protein and glucose) of antenatal was feasible. The dipslide culture method was affordable and user-friendly and allowed on-site inoculation and easy transport; challenges were contamination (midstream urine sampling) and the semi-quantitative reading. Conclusions: The E. coli isolates obtained from healthy pregnant women had significantly lower AMR rates compared to clinical E. coli isolates, probably reflecting the lower antibiotic pressure in the pregnant women population. Provided confirmation of the present findings in other settings, E. coli from urine samples in pregnant women may be a potential indicator for benchmarking, comparing, and monitoring community AMR rates across populations over different countries and regions.

Pathogenic features of urinary Escherichia coli strains causing Asymptomatic Bacteriuria during Pregnancy

2020 ◽  
Author(s):  
Samane Mohebi ◽  
zahra Hashemizade ◽  
Mahtab Hadadi ◽  
Soudeh Kholdi ◽  
Kasra Javadi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Asymptomatic Bacteriuria ◽  
Urine Samples ◽  
Diffusion Method ◽  
Low Birth Weight Infants ◽  
Phylogenetic Groups ◽  
Disk Diffusion Method ◽  
Cross Sectional ◽  
E Coli

Abstract Background Asymptomatic bacteriuria is one of the common problems in pregnancy. Pyelonephritis, preterm labor and low birth weight infants have been associated with bacterial infection. Urinary tract infection (UTI) during pregnancy is frequently associated with complications. An observational cross-sectional study including investigated the prevalence of virulence genes, antimicrobial resistance, and its relationship with phylogenetic groups among E. coli strains isolated from pregnant women with asymptomatic bacteriuria who referred to Hafez hospital, Shiraz, Iran.Material and Methods A total of 300 urine samples were screened for Escherichia coli strains. Susceptibility testing was determined by the disk-diffusion method. The phylogenetic groups and 13 virulence genes were identified by PCR. ESBL and AmpC producing isolates were detected using phenotypic methods. PCR was used to identify the bla TEM , bla SHV and bla CTXM genes in ESBL and AmpC-positive isolates.Results Our results revealed that among 300 urine samples, 105 (35%) were positive for E. coli . The data showed that the highest and the lowest resistance rates were observed against nalidixic acid (82.1%), and imipenem (2.8%), respectively. The prevalence of ESBLs and AmpC-β-lactamase, in the E. coli isolates was 41% and 9.5% respectively. bla CTXM was the commonest genotype (93%). Phylogenetic group distribution was as follow: B1 2.8%, A 14.2%, B2 61.9%, and D 4.6%. Our result showed that most of the virulence genes belonged to group B2 and also several virulence genes such as hlyA , cnf-1 , and papGII genes were positively associated with group B2. Conclusion Among E. coli strains isolated from patients with UTIs, different features phylogroups, with special virulence factors, could cause severe infection. Awareness about the Virulence patterns distribution among Phylogenetic groups of UPEC could greatly aid in confine and prevent the development of lethal infection caused by these strains.

scholarly journals Burden of Asymptomatic Bacteriuria Amongst Nigerian Pregnant Women: A Protocol for Systematic Review and Meta-Analysis

2021 ◽  
Author(s):  
Daniel N Onwusulu ◽  
Helen Chioma Okoye ◽  
Emmanuel O Nna ◽  
Samuel Onuka ◽  
Amaka Obiageli Nnamani ◽  
...  
Keyword(s):  
Risk Factors ◽  
Systematic Review ◽  
Pregnant Women ◽  
Pregnancy Outcomes ◽  
Meta Analysis ◽  
Asymptomatic Bacteriuria ◽  
Cochrane Library ◽  
Categorical Variables ◽  
Pooled Prevalence ◽  
Associated Risk Factors

Abstract BackgroundAsymptomatic bacteriuria can be a cause of adverse pregnancy and neonatal outcomes if undetected and untreated. Pregnant women are usually routinely screened with urine cultures at antenatal booking. However, the exact burden of asymptomatic bacteriuria in Nigeria is unknown. Our protocol is aimed at determining the pooled prevalence of asymptomatic bacteriuria amongst Nigerian pregnant women as well as the associated risk factors and pregnancy outcomes.MethodsNine databases: PubMed, African Journal Online, Google Scholar, Cochrane Library, CINAHL, Embase, ResearchGate, Scopus, and Web of Science will be searched using a search strategy that is developed by combinations of MeSH terms, keywords, text words, and entry terms. Only observational studies published or retrievable in the English Language will be included. Studies must be conducted in Nigeria. The primary measurable outcome of this study is the prevalence of asymptomatic bacteriuria in pregnant women. Identified studies will be screened, selected, and deduplicated in DistillerSR. Data items will be extracted into predefined forms in the DistillerSR. Reports including Prisma flow chart, quality scores, risk of bias, and study outcomes will be generated in DistillerSR. Extracted data items will be exported into the Comprehensive Meta-analysis Software version 3 for quantitative analysis. Methodological, clinical, and statistical heterogeneity will be assessed for all the studies. Publication bias will be assessed using Funnel plots. There will be a subgroup analysis of pooled prevalence using categorical variables. The primary outcome will be expressed in pooled prevalence, standard error, variance, and 95% CI of variance. Quantitative risk factors and pregnancy outcomes will be used used for meta-regression. The reporting of the systematic review and meta-analysis will be according to PRISMA 2015 Statement.DiscussionThe pooled prevalence of asymptomatic bacteriuria in Nigeria will be examined in relation to associated risk factors and pregnancy outcomes. The study will be published in a peer-reviewed scientific journal.Trial Registration NumberThis protocol is registered with the Prospective Register of Systematic Reviews (PROSPERO) with registration number CRD42020213810

scholarly journals THE CURRENT STATE OF ANTIBIOTIC RESISTANCE AND THE COMPOSITION OF PATHOGENS OF URINARY TRACT INFECTIONS IN PREGNANT WOMEN

2018 ◽  
Vol 6 (2) ◽  
pp. 13-20 ◽  
Author(s):  
K. L. Lokshin ◽  
V. N. Shirshov ◽  
A. S. Popko ◽  
Yu. L. Demidko ◽  
N. D. Luchenkova
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Antibiotic Therapy ◽  
Pregnant Women ◽  
Urinary Tract Infections ◽  
Asymptomatic Bacteriuria ◽  
Resistance Rate ◽  
Acute Cystitis ◽  
E Coli ◽  
Tract Infections

Introduction.The main treatment component of asymptomatic bacteriuria, acute cystitis and pyelonephritis in pregnant women is antibiotic therapy, which in many patients is prescribed empirically. For successful selection of the drug, it is necessary to know both the structure of pathogens and the current profile of their antibiotic resistance.Purpose of research.The study of species composition and resistance to antibiotics of bacteria that cause urinary tract infections (UTIs) in pregnant women in the Moscow RegionMaterials and methods.The study included 104 pregnant women with uncomplicated UTIs who were observed and treated at the Lapino Clinical Hospital between 2016 and 2017. The material for bacteriological studies was the midstream portion of urine or urine collected by a catheter.Results.90 patients had asymptomatic bacteriuria, 10 had acute cystitis, and 4 had acute gestational pyelonephritis. The structure of the pathogens of UTI is presented: E. coli, Enterococcus faecalis, Klebsiella pneumoniae, Proteus spp., Staphylococcus spp., Streptococcus spp., Enterobacter cloacae. The most frequently detected pathogens were E. coli (67.3%) and E. faecalis (50%). Resistance rate of E.coli strains more than 20% was detected to ampicillin (36.4%), amoxicillin / clavulanate (23.2%), trimethoprim / sulfamethoxazole (27.4%), nalidixic acid (20.7%), cephalosporins 2 and 3 generation (respectively, 25.7% and 24.3%). Resistance rate more than 20% in Enterobacteriaceae family strains was detected to trimethoprim/sulfamethoxazole (24.4%), nalidixic acid (20.7%), cephalosporins 2 generations (21.7%). Antibiotic resistance of E.coli and other Enterobacteriaceae family taxons less than 10% was noted only with respect to carbapenems (0%) and fosfomycin (1.5% and 3.5%, respectively).Conclusions.It is expedient to use the obtained data on the composition and sensitivity profile of uropathogens in UTIs in pregnant women when choosing starting empirical antibiotic therapy. Disclosure: The study did not have sponsorship. The authors have declared no conflicts of interest.

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