scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF KIWI FRUIT

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
A Siddique ◽  
N Idrees ◽  
M Kashif ◽  
R Ahmad ◽  
A Ali ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Aspergillus Ochraceus ◽  
Aspergillus Versicolor ◽  
Kiwi Fruit ◽  
Natural Form ◽  
Processed Form ◽  
Antibacterial And Antioxidant Activity

The kiwi fruit has been drawing attention and a great deal of interest because of its health benefits. It is consumed in its natural form, while it is being presented in processed form by the food industry such as sweets, ice creams, frozen juice or pulp, and many other byproducts. The peel of kiwi fruit which is a byproduct of fruit is still under exploration, but it has raised much interest in this by-product because this has many bioactive molecule contents in it such as phenolic compounds. Kiwi fruit has shown antimicrobial activity apart from the antioxidant activity against many pathogenic bacteria i.e. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes as well as fungi like Penicillium funiculosum, Aspergillus niger, Aspergillus versicolor and Aspergillus ochraceus. With the presence of antibacterial, antifungal, and antioxidant activities in kiwi fruit, it may be used as a potential medicinal fruit.

Antibacterial and antioxidant activity of wild-growing Angelica species (Apiaceae) from Balkan Peninsula against human pathogenic bacteria

2020 ◽  
Vol 32 (5) ◽  
pp. 464-473
Author(s):  
Nemanja Stanković ◽  
Tatjana Mihajilov-Krstev ◽  
Bojan Zlatković ◽  
Vesna Stankov-Jovanović ◽  
Branislava Kocić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Balkan Peninsula ◽  
Human Pathogenic Bacteria ◽  
Antibacterial And Antioxidant Activity

scholarly journals Antibacterial and Antioxidant Activities of Derriobtusone A Isolated fromLonchocarpus obtusus

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mayron Alves Vasconcelos ◽  
Francisco Vassiliepe Sousa Arruda ◽  
Daniel Barroso de Alencar ◽  
Silvana Saker-Sampaio ◽  
Maria Rose Jane Ribeiro Albuquerque ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Test Results ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
E Coli ◽  
Planktonic Growth ◽  
Ferric Reducing Antioxidant Power

This study evaluated the effect of derriobtusone A, a flavonoid isolated fromLonchocarpus obtusus, on two important pathogenic bacteria,Staphylococcus aureusandEscherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, andβ-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth ofS. aureusat 250 and 500 μg/mL; however, it did not have the same activity onE. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) ofS. aureusbiofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass ofE. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibitingβ-carotene oxidation. The compound showed no lethality toArtemiasp. nauplii. In conclusion, derriobtusone A may be an effective molecule againstS. aureusand its biofilm, as well as a potential antioxidant compound with no toxicity.

scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF AMOMUM SUBULATUM ROXB. OF NEPAL

2017 ◽  
Vol 10 (4) ◽  
pp. 95
Author(s):  
Bimala Subba ◽  
Tank Raj Seling ◽  
Ram Chandra Kandel ◽  
Ganga Prasad Phuyal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Drug ◽  
Traditional Use ◽  
Low Altitude ◽  
Amomum Subulatum ◽  
Antimicrobial And Antioxidant Activity ◽  
Antibacterial And Antioxidant Activity

Objective: The present study was designed to study and compare the antibacterial and antioxidant activity of methanol extracts of Amomum subulatum Roxb. seeds collected from two different regions of Nepal.Methods: Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl assay and Antibacterial activity was measured by agar well-diffusion method.Results: Results showed that both sample extracts had interesting antibacterial activity against all test microorganisms and had remarkable radical scavenging potential to be used as an antioxidant. Sample B (i.e., collected from high altitude Taplejung district) exerted comparative quit higher antimicrobial and antioxidant activity than the Sample A (i.e., is collected from low altitude Panchthar district). However, the standard antibacterial drug Tetracycline exhibits superior activity than the extracts.Conclusions: The results suggest that both samples (i.e., seed extract of A. subulatum Roxb.) possess almost similar moderate antibacterial and antioxidant activity in comparison to standards which justifies the traditional use of this plant.Keywords: Extract, Cardamom, Activity, Medicinal plants, Seeds, Bacillus pumilus.

scholarly journals Evaluation of antibacterial and antioxidant activities of three types of benzoin resin

2018 ◽  
Vol 9 (4) ◽  
pp. 408-411
Author(s):  
Zineb Hacini ◽  
Fatima Khedja ◽  
Ibrahim Habib ◽  
Zaouia Kendour ◽  
Zineb Debba
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Ic50 Values ◽  
Therapeutic Properties ◽  
Resin Extract ◽  
And Staphylococcus Aureus

The benzoin resin is used extensively in traditional medicine for its many reported therapeutic properties. The essential oils of three different types of benzoin resin were extracted using the traditional method in this study. The yield of essential oils of the white, red and gray types of resin was 1.01, 0.92 and 0.54%, respectively. The obtained extracts were tested against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. The tests showed that essential oil of gray type resin is effective against both Escherichia coli (14 mm) and Staphylococcus aureus (11 mm). The antioxidant activity has been also evaluated to compare the efficiency of different type of resin with DPPH· assay. In the DPPH· system, the antioxidant activity of the red resin extract (0.01 μg/mL) was superior to that of the white (27.32 μg/mL) and gray (42.90 μg/mL) extracts, with IC50 values, respectively.

scholarly journals Evaluation of the Antibacterial and Antioxidant Activity of Mimosa rubicaulis and Reinwardtia indica

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Roshani Gurung ◽  
Sundar Adhikari ◽  
Kalpana Parajuli
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Antibacterial Activity ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Ic50 Value ◽  
Antibacterial And Antioxidant Activity

Many plants have the property of wound healing, but most of the people are using costly allopathic medicine for the wound. This might be due to lack of awareness about the traditional uses and lack of scientific study of ethnomedicinal plants. So, this study aimed to carry out the antibacterial and antioxidant activity of two medicinal plants which are used traditionally for wound healing activity, i.e.,Mimosa rubicaulis and Reinwardtia indica. Different parts of Mimosa rubicaulis (root, stem, and leaves) and Reinwardtia indica (flower and leaves) were used for the study. Extractions were done by maceration using ethanol as solvent. Antibacterial activity was carried out by the well diffusion method, and antioxidant activities were screened by DPPH radical scavenging and NO scavenging assays. Extract of M. rubicaulis has shown a weak zone of inhibition towards S. aureus and P. vulgaris whereas R. indica has shown no zone of inhibition towards selected bacterial strains. Leaf, root, and stem extracts of M. rubicaulis have shown potent antioxidant activity, i.e., IC50 value of 9.8 µg/ml, 10.19 µg/ml, and, 13.64 µg/ml, respectively. Similarly, leaf extract of R. indica exhibited antioxidant activity with an IC50 value of 19.73 μg/ml. The percentage inhibition of NO radicals of root and stem of M. rubicaulis and leaves of R. indica was 31%, 21%, and 22%, respectively. Out of these two plant species, M. rubicaulis had shown antibacterial activity towards selected microorganism, but antioxidant activity was shown by both plant species. These properties on above mentioned two plant species might help for the development of a marketed formulation for antibacterial and wound-healing agent since wound healing is promoted by antibacterial and antioxidant activities.

scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF A SPECIES OF ASPERGILLUS: AN ENDOPHYTIC FUNGUS OF SCHIMA WALLICHII (DC.) KORTH. LEAVES

2017 ◽  
Vol 10 (9) ◽  
pp. 361
Author(s):  
Sunrit Basu Sarbadhikary ◽  
Narayan Chandra Mandal
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Mode Of Action ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Pathogenic Fungi ◽  
Structural Features ◽  
Fungal Endophyte ◽  
Schima Wallichii

  Objective: The main goals of this study were to check the antimicrobial and antioxidant potentials of an endophytic fungal strain isolated from the leaves of Schima wallichii (DC.) Korth.Methods: The antibacterial and antifungal activities of the isolated fungal endophyte Visva-Bharati endophyte fungal (VBEF2) were checked by disc diffusion and agar well diffusion methods, respectively, against six pathogenic bacteria and four pathogenic fungi. The minimum inhibitory concentration (MIC) values and the mode of action of VBEF2 against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were determined following colony-forming units (CFU) counting method. Antioxidant activity of the isolate was studied following 2, 2-diphenyl-2- picrilhydrazyl (DPPH) reduction assay.Results: The cell-free supernatants (CFS) of VBEF2 exhibited excellent antibacterial activity against all the bacteria used. The ethyl acetate extract of the endophyte was found to have the MIC of 50 μg/ml and 150 μg/ml against S. aureus and E. coli, respectively. It showed bactericidal mode of action against both of them. The CFS of the strain VBEF2 also showed excellent activities against two animal and two plant pathogenic fungi by producing zones of inhibition in the range of 10-20 mm. In the DPPH scavenging antioxidant assay, the ethyl acetate extract of VBEF2 was found with a low IC50 value of 19.01 μg/ml. The strain VBEF2 was identified as a species of Aspergillus based on its colony morphology and structural features observed under a compound light microscope.Conclusion: The strain VBEF2 can be implemented in various fields of pharmaceutical industry as it showed multidimensional beneficial attributes such as excellent antimicrobial and antioxidant activity.

scholarly journals Antibacterial and Antioxidant Activity of Extracts from Selected Probiotic Bacteria

2015 ◽  
Vol 4 (5) ◽  
pp. 122 ◽  
Author(s):  
Richard Nyanzi ◽  
Daniel S. S. Shuping ◽  
Piet J. Jooste ◽  
Jacobus N. Eloff
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Molecular Techniques ◽  
Methanol Extracts ◽  
Extract Concentration ◽  
Freeze Dried ◽  
Probiotic Strains ◽  
Dpph Scavenging ◽  
First Time ◽  
Antibacterial And Antioxidant Activity

<p>Probiotic extracts can potentially be used as bio-preservatives and in reduction of oxidative stress. The study investigated the antibacterial and antioxidant activity of methanol extracts from freeze-dried cells of probiotic <em>Lactobacillus</em> strains identified using molecular techniques. The quantitative microplate method, which employed <em>p</em>-iodonitrotetrazolium (INT) and the method by Brand-Williams et al. (1995) were employed to investigate quantitatively the antibacterial and the antioxidant activity, respectively, of probiotic extracts. The MIC values extracts from most probiotic strains, tested against indicator bacterial pathogens, were in the range of 1.25 – 5 mg/mL while that of <em>Lb. casei</em> strain B and <em>Lc. lactis</em> subsp <em>lactis</em> strain X was at least 20 mg/mL after 24 h of incubation at 37°C. At the highest extract concentration of 20 mg/mL used in the study, <em>Lb. acidophilus</em>, <em>Lb. rhamnosus</em> and <em>Lb. casei</em> strains had 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities of 77.9 - 86.1%, 45.7 - 86.4% and 36.9 – 45.8% respectively. Quantitative antibacterial and antioxidant activities of methanol extracts from freeze-dried cells of probiotic <em>Lactobacillus</em> strains was determined for the first time.</p>

scholarly journals Expression and Characterization of Rice-produced Recombinant Porcine Lactoferrin and its Antioxidant Activities

2020 ◽  
Vol 14 (1) ◽  
pp. 94-106
Author(s):  
Kuan-Chih Lee ◽  
Kun-Ting Hsieh ◽  
Ray-Bin Chen ◽  
Wei-Chih Lin ◽  
Chang-Sheng Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Signal Peptide ◽  
Food Industry ◽  
Animal Feed ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Positive Effects ◽  
Glycosylation Sites

Background: Lactoferrin (LF) exhibits multiple beneficial biological activities and thus has been used as a health food and additive. To broaden its application in the food industry, the porcine LF (pLF) gene has been engineered into rice to produce recombinant LF (rpLF) for use as a food additive. The iron-binding and antimicrobial activities of rpLF and its positive effects on early weaned piglets have been previously evaluated, yet several features, such as the signal peptide removal, glycosylation sites and antioxidant activity of rpLF, have not been fully characterized. Objective: In this work, the rice-produced rpLF was purified and its biochemical structure and antioxidant activities characterized. Methods: HPLC, Western blot, PAS/VVL/PNA staining, Edman degradation assay, MALDI-TOF, LC-MS/MS and antioxidant activity assays were performed. Results: The results showed that this purified rpLF is a mature form of LF; its signal peptide was correctly removed, and two N-glycosylation sites located at N365 and N472 were identified. The molecular mass heterogeneity of rpLF could be eliminated by treatment with PNGase glycosidase, suggesting that different degrees of N-glycosylation occur in rpLF. A series of assays including the iron chelating activity, reducing power assay, lipid peroxidase activity and radical-scavenging activity showed that the antioxidant activity of rice-produced rpLF was equivalent to that of bovine LF. Conclusion: Rice-produced rpLF was correctly processed post-translationally and displayed antioxidant activity equivalent to that of bovine LF; thus, rice-produced rpLF can be recognized as a plant-based antioxidant to be used as a functional additive in animal feed and for the food industry.

scholarly journals Antibacterial and Antioxidant Activity of Newly Keratinolytic Bacteria, Azotobacter chroococcum B4

2020 ◽  
Vol 13 (2) ◽  
pp. 123-127
Author(s):  
Jendri Mamangkey ◽  
Dwi Suryanto ◽  
Erman Munir ◽  
Apon Zaenal Mustopa
Keyword(s):  
Antioxidant Activity ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dpph Radical Scavenging ◽  
Antibacterial And Antioxidant Activity ◽  
Antibacterial Potential

Keratinolyticbacteria of A. chrocoocum B4 was evaluated for its potential of antibacterial and antioxidant activity. Kirby-Bauer method was used to know antibacterial potential of B4 againtsStaphylococcus aureus, S. epidermidis, Bacillus cereus, Bacillus pumilus, Bacillus subtilis, Listeria monocytogenes, Proteus sp., enteropathogenicEscherichia coli, Enterobactersakazakii, and Salmonella enterica. Antioxydant test was done using DPPH radical scavenging activity assay with ascorbic acid as a controll. In this study, hydrolysate of pellet, dialysis, and fraction 25 of B4 keratinase purification of previous study was used for antibacterial and antioxidant test. The result showed that B4 hydrolysatesinhibited Gram positive pathogenic bacteria such as Staphylococcus aureus and Listeria monocytogenes, and Gram negative Enterobacter sakazakii. All hydrolysates showed to have antioxidant properties in which fraction 25 showed higher compared to that of others. This study showed poultry waste-derived keratinase of B4 might be useful as supplementary protein, antibacterial, and antioxidant in the animal feed formulations.

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