scholarly journals CHANGES OF THE CYTOKINE PROFILE DEPENDING ON THE SEVERITY OF ENDOGENOUS INTOXICATION IN MECHANICAL JAUNDICE

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
T Aripova ◽  
N Aripova ◽  
S Matmuradov ◽  
J Babadzhanov
Keyword(s):  
Breast Cancer ◽  
Surgical Treatment ◽  
Obstructive Jaundice ◽  
Proinflammatory Cytokines ◽  
Inflammatory Process ◽  
Malignant Tumors ◽  
Cytokine Profile ◽  
Second Stage ◽  
Endogenous Intoxication ◽  
Tnf Α

Aim. Study of the reaction of the cytokine pofil to the severity of the course of endogenous intoxication in patients with obstructive jaundice.Material and methods. The study involved 84 patients with obstructive jaundice (MJ) caused by malignant tumors of the biliopancreatoduodenal zone (BPDZ). There were 55 men (65.5%), women - 29 (34.5%). The average age of the patients was 59.1 ± 2.3 years. The severity of endogenous intoxication and the cytokine profile of patients with breast cancer were assessed by determining the concentration of medium molecular peptides (MMP) and proinflammatory cytokines TNF-α and IL-6 in blood plasma and bile.Conclusion. In patients with breast cancer of tumor genesis, there is a multiple increase in the concentration of proinflammatory cytokines in blood serum and bile. There is also a manifold increase in apoptosis indices of blood lymphocytes and the content of MMP. Adequate and timely decompression of the biliary tract in breast cancer ensures the elimination of factors that cause endogenous intoxication and an increase in the inflammatory process, which makes it possible to prepare the patient for the second stage of radical surgical treatment after of the aplication of a percutaneous-transhepatic cholangiostomy.

scholarly journals Vacuum-assisted aspiration biopsy in mammology practice. Analysis of 3-year experience

2020 ◽  
Vol 16 (2) ◽  
pp. 19-24
Author(s):  
S. S. Skurikhin ◽  
Yu. V. Suvorova
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Surgical Treatment ◽  
Ultrasound Guidance ◽  
Malignant Tumors ◽  
Aspiration Biopsy ◽  
Practice Analysis ◽  
High Diagnostic Accuracy ◽  
Ultrasound Monitoring ◽  
Residual Tissue

Objective: to analyze the 3-year experience of vacuum-assisted aspiration biopsy under ultrasound guidance in patients with focal mammary neoplasms in categories BIRADS 2–5.Materials and methods. The totals of 121 interventions were performed in 121 patients. In 9 (7.4 %) cases there were more than 1 node in the mammary gland.Results. In 97.7 % cases the removed formations were benign. The breast cancer was detected in 5 (2.3 %) cases; there were cases of malignant tumors in each category according to the BIRADS scale. The complications were registered in 15 (12.3 %) cases and did not require surgical treatment. In 77 (63.3 %) cases the results of ultrasound monitoring were obtained in 6 months after the removal of formations, residual tissue was detected in 6 (4.9 %) patients.Conclusions. The vacuum-assisted aspiration biopsy, thus, has a high diagnostic accuracy; the number of completely removed benign formations allows us to consider the method as an alternative to sectoral resection. It is worth saying that a high percentage of complications is associated with the stage of mastering the method; all complications were resolved conservatively.

scholarly journals Features of a necrotic and inflammatory process in different forms of nonalcoholic fatty liver disease

2017 ◽  
Vol 89 (2) ◽  
pp. 52-58 ◽  
Author(s):  
I V Kurbatova ◽  
O P Dudanova
Keyword(s):  
Gene Expression ◽  
Liver Disease ◽  
Fatty Liver ◽  
Proinflammatory Cytokines ◽  
Fatty Liver Disease ◽  
Inflammatory Process ◽  
Caspase 3 ◽  
Control Group ◽  
Nonalcoholic Fatty Liver ◽  
Tnf Α

Aim. To identify the features of development of a necrotic and inflammatory process in different forms of nonalcoholic fatty liver disease (NAFLD), by comparatively analyzing a full set of clinical and laboratory parameters, including the cytokine status and the expression level of enzyme genes controlling the apoptosis of peripheral leukocytes. Subjects and methods 86 patients with NAFLD, including 8 (9.3%) with hepatic steatosis (HS), 70 (81.4%) with nonalcoholic steatohepatitis (NASH), 40, 19, and 11 with mild, moderate, and high disease activity, respectively, and 8 (9.3%) with liver cirrhosis (LC), were examined. A control group consisted of 34 healthy donors. Clinical and biochemical blood indices, cytokine profile, and the level of caspase gene transcripts in the peripheral blood leukocytes (PBL) were estimated. Results. As compared to the controls, the patients with HS had higher tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) levels and lower caspase 3, 6, and 8 mRNA in PBL. The concentration of IL-10 in NASH was higher than that in steatosis and positively correlated with the level of proinflammatory cytokines. The levels of TNF-α and IL6 were higher in the patients with NASH than in the controls. Those of C-reactive protein, γ-globulin, IL-6, and cytokeratin-18 fragment increased with the progression of NASH. In the latter, the transcriptional activity of caspase-3 gene decreased relative to the reference value and negatively correlated with the level of proinflammatory cytokines. In the patients with LC, the gene expression profile of caspases in PBL was similar to that in the control group; the level of IL-6 was higher than that in steatosis and NASH, that of IL-1β was higher than in HS and positively correlated the concentration of IL-6 and the activity of alanine aminotransferase and aspartate aminotransferase. Conclusion. The features of a necrotic and inflammatory process were identified in different forms of NAFLD. When the latter progressed, the cytokine profile and gene expression levels of caspases in PBL altered along with a change in the general clinical picture.

scholarly journals Palliative surgical treatment for liver metastases arising from breast cancer

2020 ◽  
Author(s):  
Katsuhisa Enomoto
Keyword(s):  
Breast Cancer ◽  
Surgical Treatment ◽  
Obstructive Jaundice ◽  
Liver Metastases ◽  
Intrahepatic Bile Duct ◽  
Left Breast ◽  
Biliary Stent ◽  
Biliary Stenting ◽  
Multiple Liver Metastases

Introduction: Patients with liver metastases arising from breast cancer presenting with jaundice have poor prognoses; most patients are not treated aggressively. However, we report an improvement in the quality of life (QOL) of the patient by inserting a biliary stent as palliative surgical treatment.Case presentation: The patient was a 63-year-old woman. She had left breast cancer and had undergone total mastectomy and axillar lymph node dissection (Bt+Ax) approximately 20 years ago. Thereafter, chemotherapy and hormonal therapy were continued for approximately 5 years. Sixteen years after the surgery, the patient presented with hepatic failure; furthermore, total bilirubin (T-Bil) levels had increased to 5.5 mg/dl. Imaging revealed multiple liver metastases and dilatation of the intrahepatic bile duct. A biliary stent was placed, and treatment for obstructive jaundice was administered. After 3 months, the patient was able to maintain QOL without any increase in T-Bil levels.Conclusion: Palliative surgical treatment via biliary stenting for the onset of obstructive jaundice due to liver metastases arising from breast cancer can be useful for maintaining patient QOL.

scholarly journals Clinical and experimental justifi cation for the use of high-frequency electrocoagulator EK-300M1 for the surgical treatment of breast tumors in female dogs

2020 ◽  
pp. 129-139
Author(s):  
S. Rublenko ◽  
A. Yaremchuk ◽  
V. Vlasenko
Keyword(s):  
Breast Cancer ◽  
Surgical Treatment ◽  
Postoperative Period ◽  
High Frequency ◽  
Breast Tumors ◽  
Early Postoperative Period ◽  
Surgical Removal ◽  
Duration Of Treatment ◽  
Objective Criterion ◽  
Endogenous Intoxication

Modern medical practice shows that the only eff ective treatment for breast cancer in dogs is surgery; current developments in chemotherapy and radiation therapy are ineff ective and too expensive. To identify the types of breast tumors and their anatomical distribution, it is necessary to have a reliable histological diagnosis. In most cases, it is used because the main treatment for breast cancer in dogs is their surgical removal. Undoubtedly decisive for this method of treatment are the phenomena of intoxication and endotoxicosis. Endotoxicosis in the early postoperative period consists of iatrogenic, tumor with tumors isolated and based on a small number of observations. However, the results of intoxication and the consequences of massive surgical tissue damage are often a determining factor for the outcome of treatment. Unfortunately, reports of studies of MSM in animals obtained by us indicate that almost all animals with breast tumors have endogenous intoxication, which we detected by the level of MSM and MDA in blood plasma. In our opinion, determination of the level of these markers of endotoxicosis before surgery and in the postoperative period can be an objective criterion for the risk of surgical treatment. Monitoring the level of MSM in the remote postoperative period, in combination with other biochemical studies can be a criterion for assessing the general clinical condition of the animal for the development of metastases. Probably lower levels of endogenous intoxication and clinically confi rmed reduction in the duration of treatment are a clear confi rmation of the eff ectiveness of high-frequency electrocoagulator EK-300M1. Dogs with breast tumors had high levels of MSM and MDA, which is a refl ection of the level of endogenous intoxication and may be an objective criterion for the risk of surgical treatment and postoperative recovery. Progressive reduction of these indicators in animals of the experimental group proves the eff ectiveness of the proposed treatment tactics. Key words: tumors, electrocoagulator EK-300M1, mastectomy, female dogs, oncology, endogenous intoxication, wound healing.

scholarly journals The role of cytokines in the pathogenesis of infectious complications in surgical treatment of obstructive jaundice of gallstone origin

2021 ◽  
Vol 20 (3) ◽  
pp. 105-111
Author(s):  
O. V. Smirnova ◽  
N. G. Elmanova
Keyword(s):  
Surgical Treatment ◽  
Obstructive Jaundice ◽  
Blood Serum ◽  
Proinflammatory Cytokines ◽  
Postoperative Period ◽  
Infectious Complications ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
High Concentrations

Aim. To study the content of cytokines in the blood serum of patients with obstructive jaundice of gallstone origin before and after surgical treatment, depending on the development of postoperative complications.Materials and methods. The treatment group consisted of 70 patients with the diagnosis of obstructive jaundice of gallstone origin, verified following a comprehensive clinical and instrumental examination. In 54 patients, the postoperative period was uncomplicated, and in 16 patients, various infectious complications in the postoperative period were revealed. The control group consisted of 125 healthy volunteers. The concentration of six cytokines (interleukin (IL)-2, IL-4, IL-18, IL-10, tumor necrosis factor alpha (TNFα), and interferon gamma (INF γ)) was determined using reagent kits manufactured by Vector-Best LLC (Novosibirsk, Russian Federation) by enzyme-linked immunosorbent assay on the Thermo Scientific analyzer (BioMerieux, France).Results. We identified significantly high concentrations of proinflammatory cytokines in the blood serum of patients with obstructive jaundice of gallstone origin upon admission, compared with the data obtained in the study of blood serum in the control group and in patients with obstructive jaundice after surgery. In the postoperative period in patients with obstructive jaundice without complications, the proinflammatory cytokines are significantly reduced and IL-4 is increased, whereas with the development of infectious complications, the level of proinflammatory cytokines is significantly elevated.Conclusion. In the pathogenesis of obstructive jaundice, a local inflammatory process plays an essential role. This is confirmed by statistically significant changes in the studied cytokines. The established increase in the concentration of IL-4, which has anti-inflammatory activity, indicates its importance in the mechanisms underlying the absence of infectious complications in the postoperative period of obstructive jaundice of gallstone origin. The revealed increase in the levels of IL-18, TNFα, and INFγ in the blood serum of patients suggests their role in the pathogenesis of infectious complications in the postoperative period of obstructive jaundice of gallstone origin.

scholarly journals Mucoxin (Acetogenin) Reduces Proinflammatory Cytokines in Breast Cancer

2019 ◽  
Vol 12 (1) ◽  
pp. 479-483
Author(s):  
Muhartono Muhartono ◽  
Subeki Subeki ◽  
Rizki Hanriko
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Proinflammatory Cytokines ◽  
Breast Cancer Cells ◽  
Anticancer Agent ◽  
Cancer Cell Line ◽  
Tnf Α ◽  
Induced Apoptosis ◽  
All Treatment ◽  
Mcf 7

Mucoxin is a potential compound used as an anticancer agent. Mucoxin induced apoptosis and inhibit proliferation in T47D breast cancer cells line. This study aims to determine the effect of mucoxin on proinflammatory cytokines in breast cancer. Proinflammatorycytokines play important role in the development and metastasis of cancer cells. Breast cancer cell line MCF-7 were grouped into five groups referred to mucoxin doses assays, they are 0 ng/mL; 0,1ng/mL; 0,5 ng/mL; 1 ng/mL; 5 ng/mL with three replication of each. Mucoxin was given for 48 hours. The levels of IL 6 and TNF-α assayed using ELISA methods. The results showed mucoxin decreases IL 6 levels in all treatment doses, but was not significant. Mucoxin also decreases TNF-α levels, with a significant reduction occurring at doses of 1 ng/mL and 5 ng/mL. It is suggested that mucoxin has potent to inhibit proinflammatory cytokines that play a role in the development and metastasis of breast cancer.

scholarly journals The role of proinflammatory cytokines in the development of anthracycline-induced heart failure

2020 ◽  
Vol 35 (2) ◽  
pp. 66-74
Author(s):  
A. T. Teplyakov ◽  
S. N. Shilov ◽  
A. A. Popova ◽  
E. N. Berezikova ◽  
E. V. Grakova ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Heart Failure ◽  
Proinflammatory Cytokines ◽  
Cardiac Injury ◽  
Cardiovascular Complications ◽  
Anthracycline Antibiotics ◽  
Anthracycline Therapy ◽  
Tnf Α ◽  
Group 1

Objective. To study the pathogenetic and prognostic role of cytokines (tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)) in the development of anthracycline-induced chronic heart failure (CHF).Material and Methods. A total of 176 women with breast cancer who received anthracycline antibiotics as a part of polychemotherapy regimens were examined. Upon examination, the patients in remission were divided into two groups within 12 months after the completion of chemotherapy: patients with the development of cardiotoxic remodeling (group 1, n = 52) and women with preserved cardiac function (group 2, n = 124). All patients received echocardiography study before, during, and after chemotherapy. Biochemical blood tests were done to determine the levels of TNF-α and IL-1β before chemotherapy, immediately after it, and 12 months after chemotherapy completion. Determination of polymorphisms of the TNF-α (–308G/A, rs1800629) and IL-1β genes (+3953, rs1143634) was carried out by polymerization chain reaction.Results. A higher level of TNF-α and IL-1β in group 1 was associated with the development of heart failure 12 months after the end of chemotherapy. The level of TNF-α over 7.5 pg/mL after the completion of chemotherapy allowed to predict the development of cardiovascular complications in women receiving anthracycline therapy with sensitivity of 44.2% and specificity of 75.8% (AUS = 0.600; 95% CI = 0.524–0.673; p = 0.035). The study did not reveal any significant differences in the frequency distribution for genotypes of 308G/A polymorphism (rs1800629) of the TNF-α gene and +3953 (rs1143634) polymorphism of the IL-1β gene in the studied groups.Conclusion. Patients with breast cancer who developed anthracycline-induced heart failure 12 months after the end of chemotherapy had the increased levels of TNF-α and IL-1β suggesting the pathogenetic role of proinflammatory cytokines in the development of cardiac injury during anthracycline therapy. 

scholarly journals Comparative Investigation of Frankincense Nutraceuticals: Correlation of Boswellic and Lupeolic Acid Contents with Cytokine Release Inhibition and Toxicity against Triple-Negative Breast Cancer Cells

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2341 ◽  
Author(s):  
Schmiech ◽  
Lang ◽  
Ulrich ◽  
Werner ◽  
Rashan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Proinflammatory Cytokines ◽  
Triple Negative ◽  
Mononuclear Cells ◽  
Breast Cancer Cell Lines ◽  
Peripheral Blood Mononuclear ◽  
Tnf Α

For centuries, frankincense extracts have been commonly used in traditional medicine, and more recently, in complementary medicine. Therefore, frankincense constituents such as boswellic and lupeolic acids are of considerable therapeutic interest. Sixteen frankincense nutraceuticals were characterized by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS), revealing major differences in boswellic and lupeolic acid compositions and total contents, which varied from 0.4% to 35.7%. Frankincense nutraceuticals significantly inhibited the release of proinflammatory cytokines, such as TNF-α, IL-6, and IL-8, by LPS-stimulated peripheral blood mononuclear cells (PBMC) and whole blood. Moreover, boswellic and lupeolic acid contents correlated with TNF-α, IL-1β, IL-6, IL-8, and IL-10 inhibition. The nutraceuticals also exhibited toxicity against the human triple-negative breast cancer cell lines MDA-MB-231, MDA-MB-453, and CAL-51 in vitro. Nutraceuticals with total contents of boswellic and lupeolic acids >30% were the most active ones against MDA-MB-231 with a half maximal inhibitory concentration (IC50) ≤ 7.0 µg/mL. Moreover, a frankincense nutraceutical inhibited tumor growth and induced apoptosis in vivo in breast cancer xenografts grown on the chick chorioallantoic membrane (CAM). Among eight different boswellic and lupeolic acids tested, β-ABA exhibited the highest cytotoxicity against MDA-MB-231 with an IC50 = 5.9 µM, inhibited growth of cancer xenografts in vivo, and released proinflammatory cytokines. Its content in nutraceuticals correlated strongly with TNF-, IL-6, and IL-8 release inhibition.

scholarly journals Role of EspA and Intimin in Expression of Proinflammatory Cytokines from Enterocytes and Lymphocytes by Rabbit Enteropathogenic Escherichia coli-Infected Rabbits

2005 ◽  
Vol 73 (1) ◽  
pp. 103-113 ◽  
Author(s):  
Karina Ramirez ◽  
Rocio Huerta ◽  
Eric Oswald ◽  
Carlos Garcia-Tovar ◽  
Jose Manuel Hernandez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Proinflammatory Cytokines ◽  
Mucosal Immune Response ◽  
Cytokine Profile ◽  
Mucosal Inflammation ◽  
Watery Diarrhea ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Spleen Lymphocytes

ABSTRACT Enteropathogenic Escherichia coli (EPEC) produces attaching and effacing (A/E) lesions and watery diarrhea, both of which are intimin and EspA dependent. In this work, we explored the mucosal immune response by detecting cytokine induction in rabbits with diarrhea caused by rabbit EPEC (REPEC). Orally inoculated rabbits exhibited weight loss and mucosal inflammation, developed watery diarrhea, and died (day 7). At day 6 postinoculation, animals were analyzed for the induction of proinflammatory cytokines in enterocytes. The role of lymphocyte-dependent immunity was determined through the expression of proinflammatory cytokines by lymphocytes from Peyer's patches (PP) and the spleen. EspA and intimin mutants were used to explore the role of A/E lesions in the expression of these cytokines. REPEC-infected rabbit enterocytes showed increased interleukin 1β (IL-1β), IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) mRNA expression, but that of anti-inflammatory IL-10 was increased only slightly. In contrast, intimin mutant-infected rabbits were unable to produce this proinflammatory cytokine profile but did produce a remarkable increase in IL-10 expression. Bacteria lacking EspA increased the expression of IL-8 and TNF-α, but that of IL-10 was increased only slightly. PP lymphocytes also produced proinflammatory cytokines, which were dependent on EspA (except for TNF-α) and intimin, while IL-10 was induced by EspA and intimin mutants. In contrast, spleen lymphocytes (systemic compartment) were unable to produce IL-1β and TNF-α. These data show the importance of the proinflammatory cytokines secreted by enterocytes and those expressed locally by PP lymphocytes, which can activate effector mechanisms at the epithelium. Furthermore, this cytokine profile, including IL-6 and IL-1β, which may be involved in the diarrhea produced by EPEC, depends on intimin.

Effects of Aerobic Exercise on Cytokine Expression in a Breast Cancer Mouse Model

2020 ◽  
Author(s):  
Bangsub LEE ◽  
Wooyoung CHUNG
Keyword(s):  
Breast Cancer ◽  
Physical Activity ◽  
Mrna Expression ◽  
Inflammatory Process ◽  
Moderate Intensity ◽  
Moderate Intensity Exercise ◽  
Necrosis Factor Alpha ◽  
Expression Levels ◽  
Tnf Α ◽  
Mrna Expression Levels

Abstract Background: Regular physical activity lowers or prevents the risk of heart disease, diabetes, some cancers, the development of hypertension, and death from these diseases through a reduction in inflammation. Cytokines, such as interleukin (IL)-6, IL-18, tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) are major markers representing the inflammatory process. This study aimed to investigate cytokine mRNA expression levels of IL-6, IL-18, TNF-α, and CRP in hepatocytes from breast cancer xenograft mice with or without moderate exercise. Methods: Each of the 5 mice at SP Korea Company, Seoul, Korea in 2015 were randomly divided into 3 groups: control (CTL), breast cancer (BC), and breast cancer exercise (BCEX). The inflammatory markers were analyzed in 10-week-old female Balb/C nude mice hepatocytes (n = 15; CTL = 5, BC = 5, BCEX = 5). Moderate intensity physical activity in mice was performed on a treadmill at an intensity of 18 m/min for 12 weeks, at 30 min for 5 days per week. Results: IL-6, IL-18, TNF-α, and CRP mRNA expression levels of the BCEX group were significantly decreased compared to those of the BC group (P < 0.05), with no difference to the CTL group. Conclusion: There might be a reduced inflammatory process via a reduction in TNF-α, IL-6, IL-18, and CRP expression in breast cancer mice that were subjected to moderate intensity exercise.

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