scholarly journals KHẢO SÁT HOẠT TÍNH ỨC CHẾ QUÁ TRÌNH PEROXIDE HÓA LIPID CỦA CÁC CHỦNG VI NẤM NỘI SINH PHÂN LẬP TỪ CÂY TRANG Kandelia candel Ở RỪNG NGẬP MẶN CẦN GIỜ

2020 ◽  
Vol 17 (12) ◽  
pp. 2273
Author(s):  
Trần Thị Minh Định ◽  
Nguyễn Ngọc Phương ◽  
Nguyễn Thị Hằng ◽  
Dương Thúc Huy

 Cây Trang rừng ngập mặn đã được chứng minh giàu các hợp chất phenol và flavonoid nên có hoạt tính kháng oxy hóa mạnh. Chính vì vậy, vi nấm nội sinh phân lập từ cây Trang có tiềm năng tạo ra chất kháng oxy hóa có hoạt tính mạnh. Trong nghiên cứu này, chúng tôi phân lập vi nấm nội sinh từ lá và rễ cây Trang, khảo sát một số đặc điểm sinh học cơ bản của chúng và khảo sát hoạt tính kháng oxy hóa của cao ethyl acetate chiết xuất từ 4 chủng vi nấm nội sinh này bằng phương pháp khảo sát năng lực khử và khảo sát hoạt tính ức chế quá trình peroxide hóa lipid. Kết quả thu được 4 chủng vi nấm nội sinh từ lá và rễ cây Trang, được bảo quản trong bộ sưu tập giống của Phòng Thí nghiệm Sinh hóa – Vi sinh, Khoa Sinh học, Trường Đại học Sư phạm Thành phố Hồ Chí Minh với mã số UP-15, UP-16, UP-17, UP-18. Cao ethyl acetate của 4 chủng nấm đều thể hiện năng lực khử. Cao ethyl acetate của chủng UP-15 thể hiện hoạt tính ức chế peroxide hóa lipid với giá trị IC50 396 μg/ml. 

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
M Diab-Assaf ◽  
RI Taleb ◽  
W Shebaby ◽  
A Mansour ◽  
CJ Moussa ◽  
...  

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
RM Marçal ◽  
DM Ptak ◽  
RR Krempser ◽  
MR Krempser ◽  
SV Floresta ◽  
...  

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.


1963 ◽  
Vol 43 (3) ◽  
pp. 345-360 ◽  
Author(s):  
Stanley Kushinsky ◽  
Jane (Wu) Tang

ABSTRACT A convenient and mild procedure is described in this paper whereby free and conjugated oestrogens may be extracted from urine. The extracts containing approximately 90 per cent of the oestrogens are devoid of most of the extraneous material and may be reduced in volume to less than 1/15 of that of the urine. The procedure consists of the following steps: (1) a 10 per cent (v/v) solution of a high molecular weight secondary amine (Amberlite LA-2, Rohm and Haas) in ethyl acetate is washed with formic acid and water, (2) the oestrogens in urine (acidified to pH 2 or 3 with H2SO4) are extracted with the LA-2 solution, (3) the oestrogen fraction is back-extracted from the organic solution with dilute aqueous ammonia.


2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.


Author(s):  
Sabreen A Kamal ◽  
Ishraq A Salih ◽  
Hawraa Jawad Kadhim ◽  
Zainab A Tolaifeh

Red rose or roselle (beauty rose ) is natively known as red tea belong to Malvaceae, it is flowers use traditionally for antihypertensive hepato protective, anticancer,antidiabetic,antibacterial, cytotoxicity and antidiarreal, By preparing red tea from it's flower. In this study, we extract chemical compounds by using two solvent which are Ethanol, Ethyl acetate. so we can extract Anthocyanin which is responsible for red colour of flower with many chemical compounds. then study the effect of these extracts on 5 genera from Enterobacteriacaea which can cause diarrheae (Shigella, Salmonella, Escherichia coli, Proteus and Klebsiella ) by preparing 3 concentrations for each solvent (250, 500, 750 ) mg/ml, and control then compare with two antibiotic (Azereonam 30 mg/ml and Bacitracin 10 mg/ml ) these extracts revealed obvious inhibition zone in bacterial growth.


2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.


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