scholarly journals Seasonal Variation of Chlorophyll and Carotenoids in Leaves of Hancornia speciosa in Three Central Areas of the Cerrado of the State of Tocantins, Brazil

2018 ◽  
Vol 10 (3) ◽  
pp. 344
Author(s):  
Rafael J. de Oliveira ◽  
José Expedito C. da Silva ◽  
Davi B. das Chagas

This study aimed to evaluate the seasonal variation in concentrations of chlorophyll a, b, total chlorophyll and carotenoids in leaves of Hancornia speciosa, Gomes, during the periods of the year, relating them to the main phenological events, periods (rainy, dry and transitions) and populations evaluated. The survey was performed in three sites and the spatio-temporal analysis divided into four periods (rainy, rainy-drytransition, dry and dry-rainy transition), with 10 replicates (matrix plants). The data were collected in average intervals of 33 days from October 2014 to April 2017. The extraction and calculation of the chlorophyll and carotenoid contents of the leaves were expressed in mg/g DM, according to the equations of Arnon (1949) and Lichtenthaler (1987). There was a significant difference between the periods and sites analyzed for all pigments and their relationships. There was a greater amount of chlorophyll a than chlorophyll b; this difference was greater in the dry period. In the rainy period, we found a greater amount of total chlorophyll, carotenoids and total chlorophyll/carotenoid ratio. The behavior for the species follows that already observed for deciduous plants, closely related to water availability.

2016 ◽  
Vol 8 (3) ◽  
pp. 347-353
Author(s):  
Fisayo S. AROYEHUN ◽  
Amos M. MAKINDE ◽  
Musibau O. ISA

The present study compared the extraction of chlorophylls from selected forest mosses (Hyophila involuta and Thuidium gratum) and derived savanna moss (Archidium ohioense) using dimethylsulphoxide (DMSO) and 80% acetone. The mosses were collected from natural populations found in the Central Campus of the Obafemi Awolowo University, Ile-Ife, Nigeria. The chlorophyll extractions process followed standard methods and the absorbance of the extracts were read on spectrophotometer at wavelengths of 645 nm and 663 nm respectively. The data obtained were later subjected to appropriate statistical analysis. The results showed that DMSO was a better chlorophyll extractant for mosses than 80% acetone. Although there were significant differences in the chlorophyll a, chlorophyll a/b ratio and total chlorophyll accumulation within all three species using DMSO and 80% acetone as extractant (P < 0.05), there was no significant difference in the chlorophyll b accumulation of all the three species (P > 0.05).


Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.


1995 ◽  
Vol 27 (2) ◽  
pp. 155-160
Author(s):  
M. A. Sanchez-Hoyos ◽  
E. Manrique

AbstractThalli of Ramalina capitata were sprayed with deionized water, 10 mM solutions of KNO3 or NH4Cl, or maintained without any water supply, for 10 days under controlled conditions. The lichens without a water supply showed an increase n antheraxanthin, lutein and β-carotene. The samples treated with deionized water showed a significant increase in violaxathin, antheraxanthin and β-carotene. The lack of a significant difference in zeaxanthin could indicate the absence of de-epoxidation of violaxanthin to zeaxanthin via antheraxanthin. Those samples treated with nitrate solution showed a significant increase in all pigments except for antheraxanthin. In contrast, ammonium treatment did not induce changes compared to water alone, except that antheraxanthin decreased. The lichens treated with nitrate exhibited a significant increase in both chlorophyll a and chlorophyll b, but ammonium affected only chlorophyll b.


2020 ◽  
pp. 1876-1882
Author(s):  
Evander Alves Ferreira ◽  
Marcia Vitória Santos ◽  
Leandro Diego da Silva ◽  
Priscila Júnia Rodrigues da Cruz ◽  
Raul Ribeiro Silveira ◽  
...  

The aim of this work was to study the effect of sub-doses of herbicide nicosulfuron on the levels of chlorophyll and growth of marandu-grass in an forest-livestock integration system. The treatments consisted of eucalyptus consortium with sorghum (BRS 655 hybrid) and forage Urochloa brizantha cv. Marandu (marandu-grass) at 12×2 and 12×3 m spacings in agroforestry systems, in addition to sorghum + marandu-grass and marandu-grass in monoculture. The doses of nicosulfuron applied at the marandu-grass were 0, 15 and 30 g ha-1. At 30 and 120 days after the application of nicosulfuron, chlorophyll a and chlorophyll b were measured usinf a portable chlorophyll meter, with six measurements per plant. Afterwards, the total chlorophyll content and chlorophyll a/b were calculated. We also measured the mass of the total dry matter of marandu-grass and growth rate of the culture. Marandu-grass plants cultivated in monoculture showed higher quantities of chlorophyll and the highest growth rates. The levels of chlorophyll a, b and chlorophyll a/b and the growth rate of marandu grass were similar between systems integrated with eucalyptus (12x2 and 12x3 m). The increase of herbicide dose negatively influenced on chlorophyll content in marandu-grass in all treatments evaluated. The use of nicosulfuron in doses of 25 and 50 g L-1 reduced the growth rate of marandu grass only when grown in monoculture


2018 ◽  
Vol 20 (1) ◽  
pp. 61-67
Author(s):  
ANNA FAUZIAH ◽  
DIETRIECH G BENGEN ◽  
MUJIZAT KAWAROE ◽  
HEFNI EFFENDI ◽  
MAJARIANA KRISANTI

Fauziah A, Bengen DG, Kawaroe M, Effendi H, Krisanti M. 2019. Spatio-temporal distribution of microalgae producing chlorophyll and carotenoid pigments in Bali Strait, Indonesia. Biodiversitas 20: 61-67. The Bali Strait waters become a place for various development activities that connect Java Island and Bali Island so that it is thought to have an impact in the life of microalgae that play an important role in the wealth of their water resources. This study aims to explore the spatiotemporal distribution of marine microalgae, which has the potential to produce chlorophyll and carotenoid pigments, as well as their relationship with the environmental characteristics of the Bali Strait waters. The research was conducted at the 5 stations in the morning, midday and afternoon. The data obtained were analyzed using Principal component analysis (PCA) and Correspondence analysis (CA). The results showed that chlorophyll-a, chlorophyll-b and carotenoid were mostly contained by the species Chaetoceros gracilis, Tripos lunula at station 5 (Pang-pang Bay) in the morning, midday and afternoon, and contained by the species Fragilariopsis cylindrus, Thalassiothrix fravenfeldii at station 3 (Ketapang-Gilimanuk ferriage) in the midday and afternoon. Spatially, salinity, ammonia, nitrate, and chlorophyll-a have significantly different values between stations, while temporally the content of chlorophyll-b and carotenoid are influenced by time (in the morning, midday and afternoon).


2013 ◽  
Vol 13 (3) ◽  
pp. 148-153 ◽  
Author(s):  
Gisele Aparecida da Silva Doratti dos Santos ◽  
Edmilson Bianchini ◽  
Nelio Roberto dos Reis

Considering the seasonal variation of fruits availability in seasonal semideciduous forests, this study analyzed whether the richness of fruit species exploited by Alouatta clamitans is higher in the rainy period rather than the dry one. Plant species consumed were investigated by visualization and feces analysis, from July 2005 to June 2006, in a seasonal semideciduous forest fragment in Paraná state, Brazil. Ten species of fruits were consumed during the rainy period and 13 during the dry period: three were consumed exclusively during the rainy period; six exclusively during the dry period; and seven in months that covered both dry and rainy periods. The number of fruit species consumed during the rainy period was considerably lower than the expected richness. Moreover, among fruit species consumed during the rainy period, only four bear fruits mainly in this period of the year, the other species bear fruits in great part of it. Therefore, the selectivity of A. clamitans was mainly directed to species capable of providing fruits along the year, prioritizing these species even when other fruit sources are available.


2018 ◽  
Vol 9 (2) ◽  
pp. 282-286
Author(s):  
Daniel Baron ◽  
Gustavo Francisco Rosalin Saraiva ◽  
Talita Silveira Amador ◽  
João Domingos Rodrigues ◽  
Rumy Goto ◽  
...  

The grafting of two cucumber scions (Cucumis sativus L.)—Taiko hybrid and Tsuyoi hybrid—onto a Tropical hybrid pumpkin rootstock (Cucurbita moschata L.) was studied. The study was conducted in a greenhouse. Plants grafted using the tongue approach grafting technique were compared with ungrafted plants. We evaluated the initial establishment of grafting through anatomical cuts on the region of union graft and examining leaves’ chlorophyll index (SPAD) and their levels of chlorophyll a, chlorophyll b and total chlorophyll. The experimental design utilized randomized blocks and the data were subjected to variance analysis (ANOVA) and the Tukey Test at 5% probability. The results indicate that Taiko cucumber scions are best grafted onto Tropical pumpkin rootstock, since these exhibits early grafting establishment. Moreover, when compared to grafting using Tsuyoi cucumber scions, Taiko cucumber scions present higher SPAD index values and levels of chlorophyll a and total chlorophyll. 


2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Rasoul Khodavirdivand Keshtiban ◽  
Hassan Soltanloo ◽  
Seyedeh Sanaz Ramazanpour ◽  
Vahid Shariati ◽  
Vahid Shariati

Investigation of wheat response to salinity stress can help to better understand the effective defense mechanisms of salinity stress tolerance. For this purpose, biochemical and physiological traits related to salinity tolerance in wheat cultivars were evaluated at Gorgan University of Agricultural Sciences and Natural Resources in 2019. Experimental factors, included two wheat crop cultivars (Sarc and Chinese spring as tolerant and susceptible wheat cultivars, respectively) and sampling time series (zero or control, 24, 48, 72, and 96 h) were examined in a factorial experiment based on a completely randomized design with three replications. Salinity stress was applied with sodium chloride at a concentration of 250 mM to uniform 10-day-old seedlings at the two-leaf stage, followed by sampling of shoot tissue. The studied traits were hydrogen peroxide (H2O2 ), chlorophyll a, chlorophyll b, total chlorophyll, chlorophyllase, carotenoids, proline, and total carbohydrates. Results of analysis of variance (ANOVA) indicated significant effects of genotype, time, and interaction of genotype × time (except H2O2 and total carbohydrates) on all the studied traits. Results of interaction of genotype × time showed although the trend of changes in the studied traits, depending on the type of cultivar and the sampling time were different, but generally, the susceptible Chinese spring cultivar contained higher levels of chlorophyllase and carotenoids than the control time at the end of sampling time and also higher H2O2 levels than the Sarc tolerant cultivar, while the Sarc tolerant cultivar, on the other hand, contained higher levels of chlorophyll a, chlorophyll b, total chlorophyll, and proline than the control time at the end of sampling time and also greater total carbohydrates than the susceptible Chinese spring cultivar. The results confirm the higher capacity of the antioxidant defense system of Sarc tolerant cultivar than the susceptible Chinese spring cultivar. Therefore


Author(s):  
Agnieszka Żurawik ◽  
Dorota Jadczak ◽  
Nikolay Panayotov ◽  
Piotr Żurawik

Due to its nutritional, health-promoting and taste-related values, new cultivars are introduced every year. The aim of the study was to assess the biological value of Polish and Bulgarian cultivars of pepper grown in moderate climate conditions and collected at different degrees of maturity. Ascorbic acid, carotenoids, polyphenols, chlorophyll a, chlorophyll b, total chlorophyll and antioxidant activity (DPPH) were determined in air-dried fruit. The investigation included five Polish cultivars: Roberta, Marta Polka, Etiuda, Trapez, and Cyklon and five Bulgarian cultivars: Bulgarski Ratung, Sivriya, Kurtovska Kapiya, Delikates, and Dzuliunska Shipka. Its design involved randomised sub-blocks, with three replications comprising nine plants each. The area of a single plot was 1.44 m<sup>2 </sup>(1.2 m × 1.2 m). The study confirmed the strong antioxidant properties of pepper grown in the field, without cover, and under temperate climate conditions. The vegetable is rich in vitamin C, polyphenols, carotenoids, chlorophyll pigments, and shows high antioxidant activity. However, the biological value of pepper is cultivar-dependent and is also determined by the fruit colouration degree. The coloured fruits are richer in vitamin C and carotenoids than the green ones, and when matured, they have greater antioxidant capacity. Green fruits contain more chlorophyll a, chlorophyll b and total chlorophyll than the coloured ones. The fruits of cv. Etiuda were the richest in vitamin C, of cv. Sivriya in polyphenols, of cv. Dzuliunska Shipka in carotenoids, and of cv. Trapez in total chlorophyll. Cv. Sivriya showed the strongest antioxidant properties.  


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